Abstract:
:Bacterial growth occurs in noncarbonated natural mineral waters a few days after filling and storage at room temperature, a phenomenon known for more than 40 years. Using the full-cycle rRNA approach, we monitored the development of the planktonic bacterial community in a noncarbonated natural mineral water after bottling. Seven 16S rRNA gene libraries, comprising 108 clones in total, were constructed from water samples taken at various days after bottling and from two different bottle sizes. Sequence analyses identified 11 operational taxonomic units (OTUs), all but one affiliated with the betaproteobacterial order Burkholderiales (6 OTUs) or the class Alphaproteobacteria (4 OTUs). Fluorescence in situ hybridization (FISH) was applied in combination with DAPI (4',6'-diamidino-2-phenylindole) staining, viability staining, and microscopic counting to quantitatively monitor changes in bacterial community composition. A growth curve similar to that of a bacterium grown in a batch culture was recorded. In contrast to the current perception that Gammaproteobacteria are the most important bacterial components of natural mineral water in bottles, Betaproteobacteria dominated the growing bacterial community and accounted for 80 to 98% of all bacteria detected by FISH in the late-exponential and stationary-growth phases. Using previously published and newly designed genus-specific probes, members of the betaproteobacterial genera Hydrogenophaga, Aquabacterium, and Polaromonas were found to constitute a significant proportion of the bacterial flora (21 to 86% of all bacteria detected by FISH). For the first time, key genera responsible for bacterial growth in a natural mineral water were identified by applying molecular cultivation-independent techniques.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Loy A,Beisker W,Meier Hdoi
10.1128/AEM.71.7.3624-3632.2005keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
3624-32issue
7eissn
0099-2240issn
1098-5336pii
71/7/3624journal_volume
71pub_type
杂志文章abstract::Graded doses of pure ochratoxin A (0, 0.5, 1.0, 2.0, 4.0, and 8.0 microgram of toxin per g of feed) were incorporated into a commercial diet which was fed to chickens from hatching to 3 weeks of age, at which time the experiments were terminated. Liver glycogen levels were elevated significantly (P less than 0.05) by ...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.37.1.122-126.1979
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2612-2617.1990
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.31.4.602-604.1976
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journal_title:Applied and environmental microbiology
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更新日期:1981-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/AEM.43.6.1473-1480.1982
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.45.3.743-747.1983
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.4.820-822.1978
更新日期:1978-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1992-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1997-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.5.1723-1727.1996
更新日期:1996-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.5.1133-1137.1986
更新日期:1986-05-01 00:00:00