Abstract:
:DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific probe hybridized to 12 of the B. thuringiensis strains examined. Most of the cryV-positive strains also hybridized to the cryI-specific probe, indicating that the cryV genes are closely related to cryI genes. Two cryV-type genes, cryV1 and cryV465, were cloned from B. thuringiensis subsp. kurstaki HD-1 and B. thuringiensis subsp. entomocidus BP465, respectively, and their nucleotide sequences were determined. The CryV1 protein was toxic to Plutella xylostella and Bombyx mori, whereas the CryV465 protein was toxic only to Plutella xylostella.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JIdoi
10.1128/AEM.61.6.2402-2407.1995subject
Has Abstractpub_date
1995-06-01 00:00:00pages
2402-7issue
6eissn
0099-2240issn
1098-5336journal_volume
61pub_type
杂志文章abstract::Both the wild type and an isogenic hydrogenase-negative mutant of Azorhizobium caulinodans growing ex planta on N(2) as the N source were studied in succinate-limited steady-state chemostat cultures under 0.2 to 3.0% dissolved O(2) tension. Production or consumption of O(2), H(2), and CO(2) was measured with an on-lin...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.60.6.1859-1866.1994
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pub_type: 杂志文章
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更新日期:1989-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.5.1980-1986.2000
更新日期:2000-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.4.1399-1407.1995
更新日期:1995-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.32.3.333-338.1976
更新日期:1976-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.2866-2872.2000
更新日期:2000-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.71.5.2592-2599.2005
更新日期:2005-05-01 00:00:00
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更新日期:1983-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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更新日期:2014-07-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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