Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials.

abstract：:This study examined the inactivation of human norovirus (HuNoV) GI.1 and GII.4 by chlorine under conditions mimicking sewage treatment. Using a porcine gastric mucin-magnetic bead (PGM-MB) assay, no statistically significant loss in HuNoV binding (inactivation) was observed for secondary effluent treatments of ≤25 ppm...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Fay JP,Calci K,Pouillot R,Woods J,Chen H,Niemira BA,Van Doren JM

更新日期：2017-11-16 00:00:00

abstract：:Of some 350 microorganisms screened, four strains of Pithomyces species were found to carry out regio-selective hydroxylation of patchoulol, a sesquiterpene, to 10-hydroxypatchoulol: Pithomyces sp. NRJ201, P. chartarum NRJ210, and, to a lesser extent, P. cynodontis ATCC 26150 and P. atro-olivaceus IFO 6651 were found ...

journal_title：Applied and environmental microbiology

authors： Suhara Y,Itoh S,Ogawa M,Yokose K,Sawada T,Sano T,Ninomiya R,Maruyama HB

更新日期：1981-08-01 00:00:00

abstract：:Campylobacter jejuni is a recognized and common gastrointestinal pathogen in most parts of the world. Human infections are often food borne, and the bacterium is frequent among poultry and other food animals. However, much less is known about the epidemiology of C. jejuni in the environment and what mechanisms the bac...

journal_title：Applied and environmental microbiology

authors： Axelsson-Olsson D,Svensson L,Olofsson J,Salomon P,Waldenström J,Ellström P,Olsen B

更新日期：2010-07-01 00:00:00

abstract：:Two Aeromonas hydrophila bacteriophages, Aeh1 and Aeh2, were isolated from sewage. Both phages showed binal symmetry. The dimensions of A. hydrophila phages Aeh1 and Aeh2 differed from those of the other Aeromonas phages. Also, phage Aeh2 was the largest Aeromonas phage studied to date. Phage Aeh1 formed small, clear ...

journal_title：Applied and environmental microbiology

authors： Chow MS,Rouf MA

更新日期：1983-05-01 00:00:00

abstract：:Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal ...

journal_title：Applied and environmental microbiology

authors： Smith RA,Couche GA

更新日期：1991-01-01 00:00:00

abstract：:Aminopolycarboxylic acids are ubiquitous in natural waters and wastewaters. They have the ability to form very stable water-soluble complexes with many metallic di- or trivalent ions. The iron complex nitrilotriacetic acid-Fe(III) (FeNTA) has been previously shown to increase drastically the rate of photo- and biodegr...

journal_title：Applied and environmental microbiology

authors： Bunescu A,Besse-Hoggan P,Sancelme M,Mailhot G,Delort AM

更新日期：2008-10-01 00:00:00

abstract：:A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for me...

journal_title：Applied and environmental microbiology

authors： Gallardo O,Fernández-Fernández M,Valls C,Valenzuela SV,Roncero MB,Vidal T,Díaz P,Pastor FI

更新日期：2010-09-01 00:00:00

abstract：:The microorganisms inhabiting a 91 degrees C hot spring in Yellowstone National Park were characterized by sequencing 5S rRNAs isolated from the mixed, natural microflora without cultivation. By comparisons of these sequences with reference sequences, the phylogenetic relationships of the hot spring organisms to bette...

journal_title：Applied and environmental microbiology

authors： Stahl DA,Lane DJ,Olsen GJ,Pace NR

更新日期：1985-06-01 00:00:00

abstract：:We investigated the effects of copper on the structure and physiology of freshwater biofilm microbial communities. For this purpose, biofilms that were grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 microM). De...

journal_title：Applied and environmental microbiology

authors： Massieux B,Boivin ME,Van Den Ende FP,Langenskiöld J,Marvan P,Barranguet C,Admiraal W,Laanbroek HJ,Zwart G

更新日期：2004-08-01 00:00:00

abstract：:Simultaneous production of penicillic acid and patulin by an atypical strain of Penicillium roqueforti isolated from cheddar cheese is reported. Mycotoxin production was confirmed by thin-layer and gas-liquid chromatography and by ultraviolet, infrared, and mass spectral analyses. Culture extracts were toxic to Bacill...

journal_title：Applied and environmental microbiology

authors： Olivigni FJ,Bullerman LB

更新日期：1978-02-01 00:00:00

abstract：:A latent virus-like agent, which we designated zooxanthella filamentous virus 1 (ZFV1), was isolated from Symbiodinium sp. strain CCMP 2465 and characterized. Transmission electron microscopy and analytical flow cytometry revealed the presence of a new group of distinctive filamentous virus-like particles after exposu...

journal_title：Applied and environmental microbiology

authors： Lohr J,Munn CB,Wilson WH

更新日期：2007-05-01 00:00:00

abstract：:To determine the potential for bacteriophage-mediated gene transfer in the marine environment, we established transduction systems by using marine phage host isolates. Plasmid pQSR50, which contains transposon Tn5 and encodes kanamycin and streptomycin resistance, was used in plasmid transduction assays. Both marine b...

journal_title：Applied and environmental microbiology

authors： Jiang SC,Paul JH

更新日期：1998-08-01 00:00:00

abstract：:The cry1-type genes of Bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. It is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. In the present study, an improved PCR-restriction frag...

journal_title：Applied and environmental microbiology

authors： Shu C,Liu D,Zhou Z,Cai J,Peng Q,Gao J,Song F,Zhang J

更新日期：2013-11-01 00:00:00

abstract：:Flux balance analysis and phenotypic data were used to provide clues to the relationships between the activities of gene products and the phenotypes resulting from the deletion of genes involved in respiratory function in Saccharomyces cerevisiae. The effect of partial or complete respiratory deficiency on the ethanol...

journal_title：Applied and environmental microbiology

authors： Dikicioglu D,Pir P,Onsan ZI,Ulgen KO,Kirdar B,Oliver SG

更新日期：2008-09-01 00:00:00

abstract：:Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

journal_title：Applied and environmental microbiology

authors： García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

更新日期：2003-12-01 00:00:00

abstract：:Ciprofloxacin (CIP), a fluoroquinolone antibacterial drug, is widely used in the treatment of serious infections in humans. Its degradation by basidiomycetous fungi was studied by monitoring 14CO2 production from [14C]CIP in liquid cultures. Sixteen species inhabiting wood, soil, humus, or animal dung produced up to 3...

journal_title：Applied and environmental microbiology

authors： Wetzstein HG,Stadler M,Tichy HV,Dalhoff A,Karl W

更新日期：1999-04-01 00:00:00

abstract：:The biological utilization of CO(2) and H(2) for the formation of short-chain fatty acids was studied by using a mixed culture of bacteria. Optimization of a medium was carried out in continuous culture to identify limiting factors which controlled growth and production of organic acids. The optimal pH for growth and ...

journal_title：Applied and environmental microbiology

authors： Goldberg I,Cooney CL

更新日期：1981-01-01 00:00:00

abstract：:Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells....

journal_title：Applied and environmental microbiology

authors： Sanchez Z,Tani A,Kimbara K

更新日期：2013-02-01 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Vector competence refers to the ability of an arthropod to acquire, maintain, and successfully transmit a microbial pathogen. Tick-borne relapsing fever (TBRF) spirochetes are globally distributed pathogens, and most species are transmitted by argasid ticks of the genus Ornithodoros. A defining characteristic in vecto...

journal_title：Applied and environmental microbiology

authors： Krishnavajhala A,Armstrong BA,Lopez JE

更新日期：2018-10-17 00:00:00

abstract：:Several microorganisms were examined for their abilities to convert S-nicotine into nornicotine. Five microorganisms including Microsporum gypseum (ATCC 11395) produced nornicotine and three unknown metabolites. M. gypseum efficiently reduced nicotine-1'-N-oxide to nicotine, but no nornicotine was obtained when the N-...

journal_title：Applied and environmental microbiology

authors： Sindelar RD,Rosazza JP,Barfknecht CF

更新日期：1979-11-01 00:00:00

abstract：:Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...

journal_title：Applied and environmental microbiology

authors： Whitman T,DeCiucies S,Hanley K,Enders A,Woolet J,Lehmann J

更新日期：2021-01-29 00:00:00

abstract：:LeLoir glycosyltransferases are important biocatalysts for the production of glycosidic bonds in natural products, chiral building blocks, and pharmaceuticals. Trehalose transferase (TreT) is of particular interest since it catalyzes the stereo- and enantioselective α,α-(1→1) coupling of a nucleotide sugar donor and m...

journal_title：Applied and environmental microbiology

authors： Mestrom L,Marsden SR,Dieters M,Achterberg P,Stolk L,Bento I,Hanefeld U,Hagedoorn PL

更新日期：2019-04-04 00:00:00

abstract：:Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proce...

journal_title：Applied and environmental microbiology

authors： Zelle RM,de Hulster E,van Winden WA,de Waard P,Dijkema C,Winkler AA,Geertman JM,van Dijken JP,Pronk JT,van Maris AJ

更新日期：2008-05-01 00:00:00

abstract：:A cup of coffee is the final product of a complex chain of operations. Wet postharvest processing of coffee is one of these operations, which involves a fermentation that inevitably has to be performed on-farm. During wet coffee processing, the interplay between microbial activities and endogenous bean metabolism resu...

journal_title：Applied and environmental microbiology

authors： Zhang SJ,De Bruyn F,Pothakos V,Torres J,Falconi C,Moccand C,Weckx S,De Vuyst L

更新日期：2019-03-06 00:00:00

abstract：:Alteromonas sp. strain O-7 secretes several proteins in addition to chitinolytic enzymes in response to chitin induction. In this paper, we report that one of these proteins, designated MprIII, is a metalloprotease involved in the chitin degradation system of the strain. The gene encoding MprIII was cloned in Escheric...

journal_title：Applied and environmental microbiology

authors： Miyamoto K,Nukui E,Hirose M,Nagai F,Sato T,Inamori Y,Tsujibo H

更新日期：2002-11-01 00:00:00

abstract：:Vibrio vulnificus is a human and animal pathogen that carries the highest death rate of any food-borne disease agent. It colonizes shellfish and forms biofilms on the surfaces of plankton, algae, fish, and eels. Greater understanding of biofilm formation by the organism could provide insight into approaches to decreas...

journal_title：Applied and environmental microbiology

authors： Nakhamchik A,Wilde C,Rowe-Magnus DA

更新日期：2008-07-01 00:00:00

abstract：UNLABELLED:Endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. One of the most widespread endosymbionts is Wolbachia pipientis, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. Although candidate proteins that co...

journal_title：Applied and environmental microbiology

authors： Christensen S,Pérez Dulzaides R,Hedrick VE,Momtaz AJ,Nakayasu ES,Paul LN,Serbus LR

更新日期：2016-08-15 00:00:00

abstract：:A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...

journal_title：Applied and environmental microbiology

authors： van de Guchte M,van der Vossen JM,Kok J,Venema G

更新日期：1989-01-01 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00