Abstract:
:Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal B. thuringiensis pathotypes active against larvae of the orders Lepidoptera, Diptera, and Coleoptera. We propose that B. thuringiensis be considered part of the common leaf microflora of many plants.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Smith RA,Couche GAdoi
10.1128/AEM.57.1.311-315.1991keywords:
subject
Has Abstractpub_date
1991-01-01 00:00:00pages
311-5issue
1eissn
0099-2240issn
1098-5336journal_volume
57pub_type
杂志文章abstract::An anaerobic, cellulose-degrading, gram-negative rod and a gram-positive coccus, identified as Bacteroides succinogenes and Ruminococcus flavefaciens, respectively, were isolated from pig fecal samples. These organisms were isolated from cellulolytic most-probable-number dilutions which represented 4 or 6% of the viab...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.1.219-221.1984
更新日期:1984-01-01 00:00:00
abstract::Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 1...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.05226-11
更新日期:2011-09-01 00:00:00
abstract::The fungus Fusarium oxysporum produced a D-amino acid oxidase (EC 1. 4.3.3) in a medium containing glucose as the carbon and energy source and ammonium sulfate as the nitrogen source. The specific D-amino acid oxidase activity was increased up to 12.5-fold with various D-amino acids or their corresponding derivatives ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.8.3750-3753.1999
更新日期:1999-08-01 00:00:00
abstract::Bacteria able to utilize Aspon, Azodrin, Dasanit, diazinon, malathion, Orthene, parathion, Trithion, dimethoate, Dylox, methyl parathion, and Vapona as sole phosphorus sources were isolated from soil and sewage. Individual isolates used from 3 to 10 of these insecticides as sole phosphorus sources. The extent of growt...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.5.886-891.1979
更新日期:1979-05-01 00:00:00
abstract::It has recently been noted that a diversity of hyperthermophilic microorganisms have the ability to reduce Fe(III) with hydrogen as the electron donor, but the reduction of Fe(III) or other metals by these organisms has not been previously examined in detail. When Pyrobaculum islandicum was grown at 100 degrees C in a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.3.1050-1056.2000
更新日期:2000-03-01 00:00:00
abstract::A toxic substance contained in the blue-green alga Microcystis aeruginosa was purified and partially characterized. Toxic algal cells were collected from a highly eutrophic lake in Japan, and the toxin was purified by homogenization, ultrafiltration, gel filtration, and ion-exchange chromatography. The final preparati...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.4.819-822.1982
更新日期:1982-04-01 00:00:00
abstract::A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.4.967-971.1981
更新日期:1981-04-01 00:00:00
abstract::Cell size is a key ecological trait of soil microorganisms that determines a wide range of life history attributes, including the efficiency of nutrient acquisition. However, because of the methodological issues associated with determining cell sizes in situ, we have a limited understanding of how cell abundances vary...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02710-13
更新日期:2013-12-01 00:00:00
abstract::Methane is an undesirable end product of rumen fermentative activity because of associated environmental impacts and reduced host feed efficiency. Our study characterized the rumen microbial methanogenic community in beef cattle divergently selected for phenotypic residual feed intake (RFI) while offered a high-forage...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03131-13
更新日期:2014-01-01 00:00:00
abstract::This study reports on an emerging fungal disease of the edible crab, Cancer pagurus. Juvenile (prerecruit) crabs were found to be subject to this disease condition during the months of May to September at two intertidal sites in South Wales, United Kingdom. Histopathology revealed that the fungi overwhelm the host res...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02945-12
更新日期:2013-02-01 00:00:00
abstract::Magnetotactic bacteria (MTB) form intracellular chain-assembled nanocrystals of magnetite or greigite termed magnetosomes. The characterization of magnetosome crystals requires electron microscopy due to their nanoscopic sizes. However, electron microscopy does not provide phylogenetic information for MTB. We have dev...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00409-17
更新日期:2017-05-31 00:00:00
abstract::Fluctuations in ciliated protozoan concentrations were monitored in 40 individually fed crossbred heifers that were stepped up to an 85% concentrate diet either slowly (12 days) or rapidly (3 days), with or without monensin (30 ppm). Ruminal fluid was withdrawn from all animals by stomach tube at the start of the stud...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.10.3174-3178.1990
更新日期:1990-10-01 00:00:00
abstract::Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests that the history of humans consuming nisin is older than the tradition of consuming fermente...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.8.5051-5053.2004
更新日期:2004-08-01 00:00:00
abstract::Ensifer adhaerens is a soil bacterium that attaches to other bacteria and may cause lysis of these other bacteria. Based on the sequence of its small-subunit rRNA gene, E. adhaerens is related to Sinorhizobium spp. E. adhaerens ATCC 33499 did not nodulate Phaseolus vulgaris (bean) or Leucaena leucocephala, but with sy...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.7.3264-3268.2001
更新日期:2001-07-01 00:00:00
abstract::To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.5.1378-1385.1990
更新日期:1990-05-01 00:00:00
abstract::Patulin production by Penicillium griseofulvum was monitored with Sep-Pak cartridges and high-pressure liquid chromatography. Determination and quantification of this metabolite proved to be very simple, and our method saved time and a large amount of organic solvents. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.1.209-210.1986
更新日期:1986-01-01 00:00:00
abstract::We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and even...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02835-08
更新日期:2009-07-01 00:00:00
abstract::We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.6.2683-2689.2002
更新日期:2002-06-01 00:00:00
abstract::The biodegradation of polycyclic aromatic hydrocarbon pollutants is constrained, in part, by their solid physical state and very low water solubility. Searching for ways to overcome these limitations, we isolated from soil a bacterium capable of growing on pyrene as a sole source of carbon and energy. Acid-fast stain,...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.7.2311-2316.1996
更新日期:1996-07-01 00:00:00
abstract::A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is det...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.1.260-264.1983
更新日期:1983-01-01 00:00:00
abstract::The mycotoxin secalonic acid D was detected for the first time as a natural contaminant of corn dust obtained from grain storage elevators. Secalonic acid D amounts ranged from 0.3 to 4.5 ppm (0.3 to 4.5 micrograms/g), as determined by reversed-phase high-performance liquid chromatography. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.4.1007-1008.1982
更新日期:1982-10-01 00:00:00
abstract::Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.4.852-857.1985
更新日期:1985-04-01 00:00:00
abstract::This is the first description, to our knowledge, of the distribution of human polyomavirus and simian virus 40 (SV40) in urban sewage. Using a nested-PCR procedure, we report the detection of human polyomaviruses JC virus (JCV) and BK virus (BKV) but not SV40 in a high percentage of urban sewage samples obtained from ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.1.238-245.2000
更新日期:2000-01-01 00:00:00
abstract::The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.10.3639-3644.1995
更新日期:1995-10-01 00:00:00
abstract::The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.5.1234-1235.1983
更新日期:1983-11-01 00:00:00
abstract::The use of a PCR method to investigate the fate of liberated Q(beta) coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.7.2932-2933.1997
更新日期:1997-07-01 00:00:00
abstract::A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00537-07
更新日期:2007-10-01 00:00:00
abstract::Five cases of primary amoebic meningoencephalitis associated with swimming in freshwater lakes have been recorded in Florida over the past 14 years. The present study demonstrated that pathogenic Naegleria, the causative agent, is relatively widespread. Twelve of 26 lakes sampled only once yielded the amoeba. Populati...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.6.661-667.1977
更新日期:1977-12-01 00:00:00
abstract::The effects of ethyl picolinate on germination, outgrowth, and sporulation of Bacillus cereus T were studied in a synthetic medium containing glucose. Ethyl picolinate specifically inhibited at two stages, outgrowth and sporulation. The initiation of germination and cell division was not affected. The inhibition of ou...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.5.1105-1111.1977
更新日期:1977-05-01 00:00:00
abstract::Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00203-10
更新日期:2010-10-01 00:00:00