Analysis of structural and physiological profiles to assess the effects of Cu on biofilm microbial communities.

abstract：:Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal ...

journal_title：Applied and environmental microbiology

authors： Smith RA,Couche GA

更新日期：1991-01-01 00:00:00

abstract：:Sakacin G is a 37-amino-acid-residue-long class IIa bacteriocin produced by Lactobacillus sake 2512, which is encoded by the duplicated structural genes skgA1 and skgA2. Sakacin G appears to be unique and seems to be an intermediate between pediocin-like bacteriocins, according to its double-disulfide bridges required...

journal_title：Applied and environmental microbiology

authors： Simon L,Fremaux C,Cenatiempo Y,Berjeaud JM

更新日期：2002-12-01 00:00:00

abstract：:The rhizobial bacterium Bradyrhizobium japonicum functions as a nitrogen-fixing symbiont of the soybean plant (Glycine max). Plants are capable of producing an oxidative burst, a rapid proliferation of reactive oxygen species (ROS), as a defense mechanism against pathogenic and symbiotic bacteria. Therefore, B. japoni...

journal_title：Applied and environmental microbiology

authors： Donati AJ,Jeon JM,Sangurdekar D,So JS,Chang WS

更新日期：2011-06-01 00:00:00

abstract：:A point mutation (E115K) resulting in slower growth of Escherichia coli DH5alpha and XL1-Blue in minimal media was identified in the purB gene, coding for adenylosuccinate lyase (ASL), through complementation with an E. coli K-12 genomic library and serial subcultures. Chromosomal modification reversing the mutation t...

journal_title：Applied and environmental microbiology

authors： Jung SC,Smith CL,Lee KS,Hong ME,Kweon DH,Stephanopoulos G,Jin YS

更新日期：2010-09-01 00:00:00

abstract：:The model organism for polyhydroxybutyrate (PHB) biosynthesis, Ralstonia eutropha H16, possesses multiple isoenzymes of granules coating phasins as well as of PHB depolymerases, which degrade accumulated PHB under conditions of carbon limitation. In this study, recombinant Escherichia coli BL21(DE3) strains were used ...

journal_title：Applied and environmental microbiology

authors： Eggers J,Steinbüchel A

更新日期：2014-12-01 00:00:00

abstract：:A multilocus enzyme electrophoresis analysis of 96 strains of the salmonellae distinguished 80 electrophoretic types (ETs) and placed them in eight groups, seven of which correspond precisely to the seven taxonomic groups (I, II, IIIa, IIIb, IV, V, and VI) previously defined on the basis of biotype and genomic DNA hyb...

journal_title：Applied and environmental microbiology

authors： Boyd EF,Wang FS,Whittam TS,Selander RK

更新日期：1996-03-01 00:00:00

abstract：:Standard plate count (SPC) bacteria were isolated from a drinking-water treatment facility and from the river supplying the facility. All isolates were identified and tested for their resistance to six antibiotics to determine if drug-resistant bacteria were selected for as a consequence of water treatment. Among the ...

journal_title：Applied and environmental microbiology

authors： Armstrong JL,Calomiris JJ,Seidler RJ

更新日期：1982-08-01 00:00:00

abstract：:The genus Bradyrhizobium has been considered to be a taxonomically difficult group. In this study, phylogenetics and evolutionary genetics analyses were used to investigate divergence levels among Bradyrhizobium strains nodulating soybeans in China. Eleven genospecies were identified by sequence analysis of three phyl...

journal_title：Applied and environmental microbiology

authors： Zhang XX,Guo HJ,Wang R,Sui XH,Zhang YM,Wang ET,Tian CF,Chen WX

更新日期：2014-05-01 00:00:00

abstract：:A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and...

journal_title：Applied and environmental microbiology

authors： Aurell H,Catala P,Farge P,Wallet F,Le Brun M,Helbig JH,Jarraud S,Lebaron P

更新日期：2004-03-01 00:00:00

abstract：:The temperate bacteriophage BK5-T was isolated from Streptococcus cremoris BK5 by induction with mitomycin C. Electron microscopy revealed that BK5-T DNA consists of linear molecules, ranging in size from 39.7 to 46 kilobase pairs. Restriction analysis of self-ligated BK5-T DNA showed that the ends of the DNA were not...

journal_title：Applied and environmental microbiology

authors： Lakshmidevi G,Davidson BE,Hillier AJ

更新日期：1988-04-01 00:00:00

abstract：:Previous studies have demonstrated that reduced humic substances (HS) can be reoxidized by anaerobic bacteria such as Geobacter, Geothrix, and Wolinella species with a suitable electron acceptor; however, little is known of the importance of this metabolism in the environment. Recently we investigated this metabolism ...

journal_title：Applied and environmental microbiology

authors： Coates JD,Cole KA,Chakraborty R,O'Connor SM,Achenbach LA

更新日期：2002-05-01 00:00:00

abstract：:The ability of staphylococcal strains isolated from different anatomical sites in 133 healthy goats to produce toxic shock syndrome toxin 1 (TSST-1) and the presence of antibodies to this toxin in serum and milk were studied. The enzyme-linked immunosorbent assay method was used to detect both the toxin and the presen...

journal_title：Applied and environmental microbiology

authors： Valle J,Vadillo S,Piriz S,Gomez-Lucia E

更新日期：1991-03-01 00:00:00

abstract：:Rhizobiophage V, isolated from soil in the vicinity of soybean roots, was strongly lytic on Bradyrhizobium japonicum 123B (USDA 123) but only mildly lytic on strain L4-4, a chemically induced small-colony mutant of 123. Numerous bacteriophage-resistant variants were isolated from L4-4 infected with phage V; two were s...

journal_title：Applied and environmental microbiology

authors： Abebe HM,Sadowsky MJ,Kinkle BK,Schmidt EL

更新日期：1992-10-01 00:00:00

abstract：:Cellulase and xylanase cDNAs were isolated from a cDNA library of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 constructed in Escherichia coli. The cellulase cDNA (celB) was 1.8 kb long with an open reading frame (ORF) coding for a polypeptide of 471 amino acids, and the xylanase cDNA (xynA) was 1.2 kb...

journal_title：Applied and environmental microbiology

authors： Li XL,Chen H,Ljungdahl LG

更新日期：1997-02-01 00:00:00

abstract：:Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...

journal_title：Applied and environmental microbiology

authors： Rhodes AN,Fulthorpe RR,Tiedje JM

更新日期：2013-11-01 00:00:00

abstract：:We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalito...

journal_title：Applied and environmental microbiology

authors： Lee SY,Lee SS,Lyoo YS,Park HM

更新日期：2011-12-01 00:00:00

abstract：:Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early e...

journal_title：Applied and environmental microbiology

authors： Calzada J,Zamarro MT,Alcón A,Santos VE,Díaz E,García JL,Garcia-Ochoa F

更新日期：2009-02-01 00:00:00

abstract：:White rot fungi can oxidize high-molecular-weight polycyclic aromatic hydrocarbons (PAH) rapidly to polar metabolites, but only limited mineralization takes place. The objectives of this study were to determine if the polar metabolites can be readily mineralized by indigenous microflora from several inoculum sources, ...

journal_title：Applied and environmental microbiology

authors： Kotterman MJ,Vis EH,Field JA

更新日期：1998-08-01 00:00:00

abstract：:Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ...

journal_title：Applied and environmental microbiology

authors： Miller EN,Jarboe LR,Yomano LP,York SW,Shanmugam KT,Ingram LO

更新日期：2009-07-01 00:00:00

abstract：:Although Salmonella enterica can produce life-threatening colitis in horses, certain serotypes are more commonly associated with clinical disease. Our aim was to evaluate the proportional morbidity attributed to different serotypes, as well as the phenotypic and genotypic antimicrobial resistance (AMR) of Salmonella i...

journal_title：Applied and environmental microbiology

authors： Leon IM,Lawhon SD,Norman KN,Threadgill DS,Ohta N,Vinasco J,Scott HM

更新日期：2018-06-18 00:00:00

abstract：:To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers o...

journal_title：Applied and environmental microbiology

authors： Dudley DJ,Guentzel MN,Ibarra MJ,Moore BE,Sagik BP

更新日期：1980-01-01 00:00:00

abstract：:Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

journal_title：Applied and environmental microbiology

authors： Davies JS,Wellman AM,Zajic JE

更新日期：1976-07-01 00:00:00

abstract：:The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H2 plus CO2 and by increasing the CO concentration in small, 10% increments.T. kivui was finally abl...

journal_title：Applied and environmental microbiology

authors： Weghoff MC,Müller V

更新日期：2016-04-04 00:00:00

abstract：:Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was ...

journal_title：Applied and environmental microbiology

authors： Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají E

更新日期：2006-05-01 00:00:00

abstract：:The ability of Listeria monocytogenes to tolerate salt stress is of particular importance, as this pathogen is often exposed to such environments during both food processing and food preservation. In order to understand the survival mechanisms of L. monocytogenes, an initial approach using two-dimensional polyacrylami...

journal_title：Applied and environmental microbiology

authors： Duché O,Trémoulet F,Glaser P,Labadie J

更新日期：2002-04-01 00:00:00

abstract：:The capacity of polycyclic aromatic hydrocarbon (PAH)-utilizing bacteria to produce biosurfactants was investigated. Twenty-three bacteria isolated from a soil contaminated with petroleum wastes were able to form clearing zones on mineral salt agar plates sprayed with solutions of PAHs. Naphthalene and phenanthrene we...

journal_title：Applied and environmental microbiology

authors： Deziel E,Paquette G,Villemur R,Lepine F,Bisaillon J

更新日期：1996-06-01 00:00:00

abstract：:L-threonine can be made by the amino acid-producing bacterium Corynebacterium glutamicum. However, in the course of this process, some of the L-threonine is degraded to glycine. We detected an aldole cleavage activity of L-threonine in crude extracts with an activity of 2.2 nmol min(-1) (mg of protein)(-1). In order t...

journal_title：Applied and environmental microbiology

authors： Simic P,Willuhn J,Sahm H,Eggeling L

更新日期：2002-07-01 00:00:00

abstract：:The ability of Erwinia chrysanthemi to cope with environments of elevated osmolality is due in part to the transport and accumulation of osmoprotectants. In this study we have identified a high-affinity glycine betaine and choline transport system in E. chrysanthemi. By using a pool of Tn5-B21 ousA mutants, we isolate...

journal_title：Applied and environmental microbiology

authors： Choquet G,Jehan N,Pissavin C,Blanco C,Jebbar M

更新日期：2005-07-01 00:00:00

abstract：:A set of freshwater mesocosms (1.7 m3 each) was inoculated with large amounts of Escherichia coli, Pseudomonas putida, and their culture medium to substantially disturb the natural microbial community. To monitor microbial community dynamics, low-molecular-weight RNA (5S rRNA and tRNA) obtained directly from bacteriop...

journal_title：Applied and environmental microbiology

authors： Höfle MG

更新日期：1992-10-01 00:00:00

abstract：:We present a tool for repetitive, marker-free, site-specific integration in Lactococcus lactis, in which a nonreplicating plasmid vector (pKV6) carrying a phage attachment site (attP) can be integrated into a bacterial attachment site (attB). The novelty of the tool described here is the inclusion of a minimal bacteri...

journal_title：Applied and environmental microbiology

authors： Petersen KV,Martinussen J,Jensen PR,Solem C

更新日期：2013-06-01 00:00:00