Abstract:
:Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was evaluated in terms of the MIC and the 50% effective dose (ED(50)) for growth. Peptide cytotoxicity against human red blood cells and peptide stability toward protease degradation were also determined. Pep3 and several analogues inhibited growth of the three pathogens and had a bactericidal effect at low micromolar concentrations (ED(50) of 1.3 to 7.3 microM). One of the analogues consisting of a replacement of both Trp and Val with Lys and Phe, respectively, resulted in a peptide with improved bactericidal activity and minimized cytotoxicity and susceptibility to protease degradation compared to Pep3. The best analogues can be considered as potential lead compounds for the development of new antimicrobial agents for use in plant protection either as components of pesticides or expressed in transgenic plants.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají Edoi
10.1128/AEM.72.5.3302-3308.2006subject
Has Abstractpub_date
2006-05-01 00:00:00pages
3302-8issue
5eissn
0099-2240issn
1098-5336pii
72/5/3302journal_volume
72pub_type
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