Saccharomyces cerevisiae genome shuffling through recursive population mating leads to improved tolerance to spent sulfite liquor.

Abstract:

:Spent sulfite liquor (SSL) is a waste effluent from sulfite pulping that contains monomeric sugars which can be fermented to ethanol. However, fermentative yeasts used for the fermentation of the sugars in SSL are adversely affected by the inhibitory substances in this complex feedstock. To overcome this limitation, evolutionary engineering of Saccharomyces cerevisiae was carried out using genome-shuffling technology based on large-scale population cross mating. Populations of UV-light-induced yeast mutants more tolerant than the wild type to hardwood spent sulfite liquor (HWSSL) were first isolated and then recursively mated and enriched for more-tolerant populations. After five rounds of genome shuffling, three strains were isolated that were able to grow on undiluted HWSSL and to support efficient ethanol production from the sugars therein for prolonged fermentation of HWSSL. Analyses showed that greater HWSSL tolerance is associated with improved viability in the presence of salt, sorbitol, peroxide, and acetic acid. Our results showed that evolutionary engineering through genome shuffling will yield robust yeasts capable of fermenting the sugars present in HWSSL, which is a complex substrate containing multiple sources of inhibitors. These strains may not be obtainable through classical evolutionary engineering and can serve as a model for further understanding of the mechanism behind simultaneous tolerance to multiple inhibitors.

journal_name

Appl Environ Microbiol

authors

Pinel D,D'Aoust F,del Cardayre SB,Bajwa PK,Lee H,Martin VJ

doi

10.1128/AEM.02769-10

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

4736-43

issue

14

eissn

0099-2240

issn

1098-5336

pii

AEM.02769-10

journal_volume

77

pub_type

杂志文章
  • Extensive reduction of cell viability and enhanced matrix production in Pseudomonas aeruginosa PAO1 flow biofilms treated with a D-amino acid mixture.

    abstract::Treatment of Pseudomonas aeruginosa PAO1 flow biofilms with a D-amino acid mixture caused significant reductions in cell biomass by 75% and cell viability by 71%. No biofilm disassembly occurred, and matrix production increased by 30%, thereby providing a thick protective cover for remaining viable or persister cells....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02911-12

    authors: Sanchez Z,Tani A,Kimbara K

    更新日期:2013-02-01 00:00:00

  • Novel Sampling Method for Assessing Human-Pathogen Interactions in the Natural Environment Using Boot Socks and Citizen Scientists, with Application to Campylobacter Seasonality.

    abstract::This paper introduces a novel method for sampling pathogens in natural environments. It uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. Wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00162-17

    authors: Jones NR,Millman C,van der Es M,Hukelova M,Forbes KJ,Glover C,Haldenby S,Hunter PR,Jackson K,O'Brien SJ,Rigby D,Strachan NJC,Williams N,Lake IR,ENIGMA Consortium.

    更新日期:2017-06-30 00:00:00

  • Integrative conjugative elements are widespread in field isolates of Mycoplasma species pathogenic for ruminants.

    abstract::Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03723-14

    authors: Tardy F,Mick V,Dordet-Frisoni E,Marenda MS,Sirand-Pugnet P,Blanchard A,Citti C

    更新日期:2015-03-01 00:00:00

  • Solute transport proteins and the outer membrane protein NmpC contribute to heat resistance of Escherichia coli AW1.7.

    abstract::This study aimed to elucidate determinants of heat resistance in Escherichia coli by comparing the composition of membrane lipids, as well as gene expression, in heat-resistant E. coli AW1.7 and heat-sensitive E. coli GGG10 with or without heat shock. The survival of E. coli AW1.7 at late exponential phase was 100-fol...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01930-10

    authors: Ruan L,Pleitner A,Gänzle MG,McMullen LM

    更新日期:2011-05-01 00:00:00

  • Lysis of Bacillus subtilis Cells by Glycerol and Sucrose Esters of Fatty Acids.

    abstract::The lytic action of glycerol and sucrose esters of fatty acids with different carbon chain lengths on the exponentially growing cells of Bacillus subtilis 168 was investigated. Of each series of esters, glycerol dodecanoate and sucrose hexadecanoate were the most active. Lysis at 1 h after the addition of 0.1 mM glyce...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.3.505-508.1987

    authors: Tsuchido T,Ahn YH,Takano M

    更新日期:1987-03-01 00:00:00

  • Genetic diversity and fitness of Fusarium graminearum populations from rice in Korea.

    abstract::Fusarium graminearum is an important fungal pathogen of cereal crops and produces mycotoxins, such as the trichothecenes nivalenol and deoxynivalenol. This species may be subdivided into a series of genetic lineages or phylogenetic species. We identified strains of F. graminearum from the Republic of Korea to lineage,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02287-08

    authors: Lee J,Chang IY,Kim H,Yun SH,Leslie JF,Lee YW

    更新日期:2009-05-01 00:00:00

  • Stable carbon isotope fractionation by sulfate-reducing bacteria.

    abstract::Biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. Accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.5.2942-2949.2003

    authors: Londry KL,Des Marais DJ

    更新日期:2003-05-01 00:00:00

  • Molecular characterization of methanotrophic isolates from freshwater lake sediment.

    abstract::Profiles of dissolved O(2) and methane with increasing depth were generated for Lake Washington sediment, which suggested the zone of methane oxidation is limited to the top 0.8 cm of the sediment. Methane oxidation potentials were measured for 0.5-cm layers down to 1.5 cm and found to be relatively constant at 270 to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.12.5259-5266.2000

    authors: Auman AJ,Stolyar S,Costello AM,Lidstrom ME

    更新日期:2000-12-01 00:00:00

  • A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control.

    abstract::We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.9008-9012.2005

    authors: Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

    更新日期:2005-12-01 00:00:00

  • The Cellulases Endoglucanase I and Cellobiohydrolase II of Trichoderma reesei Act Synergistically To Solubilize Native Cotton Cellulose but Not To Decrease Its Molecular Size.

    abstract::Degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) was investigated by analyzing the insoluble cellulose fragments remaining after enzymatic hydrolysis. Changes in the molecular-size distribution of cellulose after attack by EGI, alone and in combination with C...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.8.2883-2887.1996

    authors: Kleman-Leyer KM,Siika-Aho M,Teeri TT,Kirk TK

    更新日期:1996-08-01 00:00:00

  • Hydroxylation and dechlorination of chlorinated guaiacols and syringols by Rhodococcus chlorophenolicus.

    abstract::We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.3.683-687.1988

    authors: Häggblom MM,Apajalahti JH,Salkinoja-Salonen MS

    更新日期:1988-03-01 00:00:00

  • Cocultivation of Legionella pneumophila and free-living amoebae.

    abstract::Studies of the interaction of Legionella pneumophila with free-living amoebae showed that Naegleria lovaniensis and Acanthamoeba royreba could use L. pneumophila as a sole food source. However, growth of the amoebae on nonnutrient agar plates seeded with L. pneumophila was slower than growth on nonnutrient agar plates...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.4.954-959.1982

    authors: Tyndall RL,Domingue EL

    更新日期:1982-10-01 00:00:00

  • 13C incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza.

    abstract::The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1omega5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant specie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.5.2592-2599.2005

    authors: Olsson PA,van Aarle IM,Gavito ME,Bengtson P,Bengtsson G

    更新日期:2005-05-01 00:00:00

  • Bacterial survival in a dilute environment.

    abstract::Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.6.1331-1336.1981

    authors: Sjogren RE,Gibson MJ

    更新日期:1981-06-01 00:00:00

  • Adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and polarized thin films of gold.

    abstract::The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.3188-3194.2001

    authors: Busalmen JP,de Sánchez SR

    更新日期:2001-07-01 00:00:00

  • Microbial activity at the sediment-water interface in halifax harbor, Canada.

    abstract::The sediment-water interface in Halifax Harbor supports a microbial population of 6.95 x 10 cells per g (dry weight). As determined by the standard technique of suspending subsamples in filtered seawater, the uptake of added glutamic acid by this population is 113.5 ng g (dry weight) h. An alternate technique was deve...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.6.1761-1766.1983

    authors: Novitsky JA

    更新日期:1983-06-01 00:00:00

  • Developmental and microbiological analysis of the inception of bioluminescent symbiosis in the marine fish Nuchequula nuchalis (Perciformes: Leiognathidae).

    abstract::Many marine fish harbor luminous bacteria as bioluminescent symbionts. Despite the diversity, abundance, and ecological importance of these fish and their apparent dependence on luminous bacteria for survival and reproduction, little is known about developmental and microbiological events surrounding the inception of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01619-08

    authors: Dunlap PV,Davis KM,Tomiyama S,Fujino M,Fukui A

    更新日期:2008-12-01 00:00:00

  • A novel insecticidal toxin from photorhabdus luminescens, toxin complex a (Tca), and its histopathological effects on the midgut of manduca sexta

    abstract::Photorhabdus luminescens is a bacterium which is mutualistic with entomophagous nematodes and which secretes high-molecular-weight toxin complexes following its release into the insect hemocoel upon nematode invasion. Thus, unlike other protein toxins from Bacillus thuringiensis (delta-endotoxins and Vip's), P. lumine...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.8.3036-3041.1998

    authors: Blackburn M,Golubeva E,Bowen D,Ffrench-Constant RH

    更新日期:1998-08-01 00:00:00

  • Inactivation of isocitrate lyase leads to increased production of medium-chain-length poly(3-hydroxyalkanoates) in Pseudomonas putida.

    abstract::Medium-chain-length (mcl) poly(3-hydroxyalkanoates) (PHAs) are storage polymers that are produced from various substrates and accumulate in Pseudomonas strains belonging to rRNA homology group I. In experiments aimed at increasing PHA production in Pseudomonas strains, we generated an mcl PHA-overproducing mutant of P...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.3.909-913.2000

    authors: Klinke S,Dauner M,Scott G,Kessler B,Witholt B

    更新日期:2000-03-01 00:00:00

  • Utilization of individual cellodextrins by three predominant ruminal cellulolytic bacteria.

    abstract::Growth of the ruminal bacteria Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and R. albus 7 followed Monod kinetics with respect to concentrations of individual pure cellodextrins (cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose). Under the conditions tested, R. flavefaciens FD-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.3.1084-1088.1996

    authors: Shi Y,Weimer PJ

    更新日期:1996-03-01 00:00:00

  • Detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis.

    abstract::Correlations between the biomass of phytoplankton and the biomass of bacteria and between the biomass of bacteria and the biomass of protozoans suggest that there is coupling between these compartments of the "microbial loop." To investigate this coupling on the species level, bacteria and protozoans from untreated la...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.6.2478-2484.1999

    authors: van Hannen EJ,Mooij W,van Agterveld MP,Gons HJ,Laanbroek HJ

    更新日期:1999-06-01 00:00:00

  • Starvation-induced thermal tolerance as a survival mechanism in a psychrophilic marine bacterium.

    abstract::Carbon-starved cultures of strain Ant-300, a psychrophilic marine vibrio isolated from the Antarctic Convergence, were compared with their nonstarved counterparts for resistance to heat. Specifically, starved and unstarved cells were exposed to 17 degrees C, which is 4 degrees C above the maximum growth temperature, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.8.2653-2656.1993

    authors: Preyer JM,Oliver JD

    更新日期:1993-08-01 00:00:00

  • Anaerobic degradation of propionate by a mesophilic acetogenic bacterium in coculture and triculture with different methanogens.

    abstract::A mesophilic acetogenic bacterium (MPOB) oxidized propionate to acetate and CO(2) in cocultures with the formate- and hydrogen-utilizing methanogens Methanospirillum hungatei and Methanobacterium formicicum. Propionate oxidation did not occur in cocultures with two Methanobrevibacter strains, which grew only with hydr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.8.2834-2838.1994

    authors: Dong X,Plugge CM,Stams AJ

    更新日期:1994-08-01 00:00:00

  • Detection of Toxoplasma gondii oocysts in drinking water.

    abstract::The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gond...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.6.2278-2280.1998

    authors: Isaac-Renton J,Bowie WR,King A,Irwin GS,Ong CS,Fung CP,Shokeir MO,Dubey JP

    更新日期:1998-06-01 00:00:00

  • Complementation of the inability of Lactobacillus strains to utilize D-xylose with D-xylose catabolism-encoding genes of Lactobacillus pentosus.

    abstract::The inability of two Lactobacillus strains to ferment D-xylose was complemented by the introduction of Lactobacillus pentosus genes encoding D-xylose isomerase, D-xylulose kinase, and a D-xylose catabolism regulatory protein. This result opens the possibility of using D-xylose fermentation as a food-grade selection ma...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2764-2766.1991

    authors: Posno M,Heuvelmans PT,van Giezen MJ,Lokman BC,Leer RJ,Pouwels PH

    更新日期:1991-09-01 00:00:00

  • Genetic and functional characterization of cyclic lipopeptide white-line-inducing principle (WLIP) production by rice rhizosphere isolate Pseudomonas putida RW10S2.

    abstract::The secondary metabolite mediating the GacS-dependent growth-inhibitory effect exerted by the rice rhizosphere isolate Pseudomonas putida RW10S2 on phytopathogenic Xanthomonas species was identified as white-line-inducing principle (WLIP), a member of the viscosin group of cyclic lipononadepsipeptides. WLIP producers ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00335-12

    authors: Rokni-Zadeh H,Li W,Sanchez-Rodriguez A,Sinnaeve D,Rozenski J,Martins JC,De Mot R

    更新日期:2012-07-01 00:00:00

  • Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by Rhodococcus erythropolis I-19.

    abstract::Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.11.4967-4972.1999

    authors: Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

    更新日期:1999-11-01 00:00:00

  • Diversity and seasonal variability of beta-Proteobacteria in biofilms of polluted rivers: analysis by temperature gradient gel electrophoresis and cloning.

    abstract::The beta-subgroup of the Proteobacteria has been shown to be important in aquatic habitats and was investigated in depth here by molecular 16S rRNA techniques in biofilms of the Elbe River and its polluted tributary, the Spittelwasser River. The bacterial 16S rRNA genes were cloned from each site, screened for beta-pr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.8.4463-4473.2003

    authors: Brümmer IH,Felske A,Wagner-Döbler I

    更新日期:2003-08-01 00:00:00

  • Biphasic thermal inactivation kinetics in Salmonella enteritidis PT4.

    abstract::The thermal inactivation kinetics of Salmonella enteritidis PT4 between 49 and 60 degrees C were investigated. Using procedures designed to eliminate methodological artifacts, we found that the death kinetics deviated from the accepted model of first-order inactivation. When we used high-density stationary-phase popul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.2.459-464.1998

    authors: Humpheson L,Adams MR,Anderson WA,Cole MB

    更新日期:1998-02-01 00:00:00

  • Sequence heterogeneity in the two 16S rRNA genes of Phormium yellow leaf phytoplasma.

    abstract::Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes of PYL phytoplasma were amplified from infected flax by PCR and cloned, and the nucleotide sequences were determined. DNA sequencing and Southern hybridization analysis of genomic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.9.3133-3139.1996

    authors: Liefting LW,Andersen MT,Beever RE,Gardner RC,Forster RL

    更新日期:1996-09-01 00:00:00