Diversity and seasonal variability of beta-Proteobacteria in biofilms of polluted rivers: analysis by temperature gradient gel electrophoresis and cloning.

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:The facultative anaerobe Thauera aromatica strain AR-1 uses 3,5-dihydroxybenzoate (3,5-DHB) as a sole carbon and energy source under anoxic conditions using an unusual oxidative strategy to overcome aromatic ring stability. A 25-kb gene cluster organized in four main operons encodes the anaerobic degradation pathway f...

journal_title：Applied and environmental microbiology

authors： Pacheco-Sánchez D,Molina-Fuentes Á,Marín P,Díaz-Romero A,Marqués S

更新日期：2019-01-09 00:00:00

abstract：:Spent sulfite liquor (SSL) is a waste effluent from sulfite pulping that contains monomeric sugars which can be fermented to ethanol. However, fermentative yeasts used for the fermentation of the sugars in SSL are adversely affected by the inhibitory substances in this complex feedstock. To overcome this limitation, e...

journal_title：Applied and environmental microbiology

authors： Pinel D,D'Aoust F,del Cardayre SB,Bajwa PK,Lee H,Martin VJ

更新日期：2011-07-01 00:00:00

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life ...

journal_title：Applied and environmental microbiology

authors： Graham ML,Springthorpe VS,Sattar SA

更新日期：1996-11-01 00:00:00

abstract：:The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

journal_title：Applied and environmental microbiology

authors： Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

更新日期：2011-04-01 00:00:00

abstract：:Four enteric viruses, poliovirus type 1, echovirus type 1, reovirus type 3, and simian adenovirus SV-11, were concentrated from seeded 1.3-liter volumes of raw, finished, and granular activated carbon-treated waters by adsorption to 47-mm-diameter (17 cm2), electropositive ( Virosorb 1MDS ) filters at pH 7.5 or electr...

journal_title：Applied and environmental microbiology

authors： Sobsey MD,Glass JS

更新日期：1984-05-01 00:00:00

abstract：:In this article, a new mechanism influencing the transport of microorganisms through unsaturated porous media is examined, and a new method for directly visualizing bacterial behavior within a porous medium under controlled chemical and flow conditions is introduced. Resting cells of hydrophilic and relatively hydroph...

journal_title：Applied and environmental microbiology

authors： Wan J,Wilson JL,Kieft TL

更新日期：1994-02-01 00:00:00

abstract：:During malolactic fermentation in wine by Oenococcus oeni, the degradation of citric acid was delayed compared to the degradation of malic acid. The maximum concentration of diacetyl, an intermediary compound in the citric acid metabolism with a buttery or nutty flavor, coincided with the exhaustion of malic acid in t...

journal_title：Applied and environmental microbiology

authors： Nielsen JC,Richelieu M

更新日期：1999-02-01 00:00:00

abstract：:Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectr...

journal_title：Applied and environmental microbiology

authors： Kawai Y,Ishii Y,Arakawa K,Uemura K,Saitoh B,Nishimura J,Kitazawa H,Yamazaki Y,Tateno Y,Itoh T,Saito T

更新日期：2004-05-01 00:00:00

abstract：:The effects of drying time during freeze-drying on the outermost cell surface of an encapsulated strain of Staphylococcus aureus S-7 (Smith, diffuse) were investigated, with special attention paid to capsule and slime production. To quantify capsule and slime production, capsule antigen production and cellular charact...

journal_title：Applied and environmental microbiology

authors： Ohtomo T,Yamada T,Yoshida K

更新日期：1988-10-01 00:00:00

abstract：:We report on the hydrogen production properties of the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142. This organism has a versatile metabolism and can grow in the presence or absence of combined nitrogen and can grow photosynthetically or mixotrophically and heterotrophically in the presenc...

journal_title：Applied and environmental microbiology

authors： Min H,Sherman LA

更新日期：2010-07-01 00:00:00

abstract：:Group I strains of Clostridium botulinum are known to degrade arginine by the arginine deiminase pathway. We have found that C. botulinum Okra B consumed a level of arginine (20 g/liter) higher than the basal requirement for growth (3 g/liter). Arginine was probably the preferred source of nitrogen for biosynthesis bu...

journal_title：Applied and environmental microbiology

authors： Patterson-Curtis SI,Johnson EA

更新日期：1992-07-01 00:00:00

abstract：:A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...

journal_title：Applied and environmental microbiology

authors： Ballesté E,Bonjoch X,Belanche LA,Blanch AR

更新日期：2010-03-01 00:00:00

abstract：:The erythromycin resistance gene ermB has been found in a variety of gram-positive bacteria. This gene has also been found in Bacteroides species but only in six recently isolated strains; thus, the gene seems to have entered this genus only recently. One of the six Bacteroides ermB-containing isolates, WH207, could t...

journal_title：Applied and environmental microbiology

authors： Gupta A,Vlamakis H,Shoemaker N,Salyers AA

更新日期：2003-11-01 00:00:00

abstract：:Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were ...

journal_title：Applied and environmental microbiology

authors： Donegan K,Fieland V,Fowles N,Ganio L,Seidler R

更新日期：1992-04-01 00:00:00

abstract：:Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo Here, we established a proof-of-concept single-homology-arm linear DNA...

journal_title：Applied and environmental microbiology

authors： Lu S,Nie Y,Wang M,Xu HX,Ma DL,Liang JL,Wu XL

更新日期：2018-09-17 00:00:00

abstract：:In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

journal_title：Applied and environmental microbiology

authors： Rahkila R,Johansson P,Säde E,Björkroth J

更新日期：2011-02-01 00:00:00

abstract：:Two PCR primer pairs, based on the virD2 and ipt genes, detected a wide variety of pathogenic Agrobacterium strains. The endonuclease domain of VirD2 protein, which cleaves transferred DNA (T-DNA) border sequences, is highly conserved; primer oligonucleotides specific for the endonuclease portion of virD2 detected all...

journal_title：Applied and environmental microbiology

authors： Haas JH,Moore LW,Ream W,Manulis S

更新日期：1995-08-01 00:00:00

abstract：:Fatty acid compositions in growing and resting cells of several strains of Pseudomonas putida (P8, NCTC 10936, and KT 2440) were studied, with a focus on alterations of the saturation degree, cis-trans isomerization, and cyclopropane formation. The fatty acid compositions of the strains were very similar under compara...

journal_title：Applied and environmental microbiology

authors： Härtig C,Loffhagen N,Harms H

更新日期：2005-04-01 00:00:00

abstract：:The existence of anaerobic biodegradation of lignin was examined in mixed microflora. Egyptian soil samples, in which rapid mineralization of organic matter takes place in the presence of an important anaerobic microflora, were used to obtain the anaerobic enrichment cultures for this study. Specifically, 14CO2 or [14...

journal_title：Applied and environmental microbiology

authors： Odier E,Monties B

更新日期：1983-09-01 00:00:00

abstract：:Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection...

journal_title：Applied and environmental microbiology

authors： de Bruin A,de Groot A,de Heer L,Bok J,Wielinga PR,Hamans M,van Rotterdam BJ,Janse I

更新日期：2011-09-01 00:00:00

abstract：:Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

journal_title：Applied and environmental microbiology

authors： Hamdan H,Weller DM,Thomashow LS

更新日期：1991-11-01 00:00:00

abstract：:H(2) generated from renewable resources holds promise as an environmentally innocuous fuel that releases only energy and water when consumed. In biotechnology, photoautotrophic oxygenic diazotrophs could produce H(2) from water and sunlight using the cells' endogenous nitrogenases. However, nitrogenases have low turno...

journal_title：Applied and environmental microbiology

authors： Gärtner K,Lechno-Yossef S,Cornish AJ,Wolk CP,Hegg EL

更新日期：2012-12-01 00:00:00

abstract：:Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages pr...

journal_title：Applied and environmental microbiology

authors： Roach DR,Sjaarda DR,Castle AJ,Svircev AM

更新日期：2013-05-01 00:00:00

abstract：:Ninety-nine Campylobacter coli isolates were examined by bacterial restriction endonuclease DNA analysis (BRENDA) with HindIII. Isolates from poultry from the same environment had identical patterns, patterns of isolates carried by suckling piglets were generally the same as those of isolates recovered from their dams...

journal_title：Applied and environmental microbiology

authors： Kakoyiannis CK,Winter PJ,Marshall RB

更新日期：1984-09-01 00:00:00

abstract：:Organically raised swine had high fecal populations of chlortetracycline (CTC)-resistant (growing at 64 μg CTC/ml) Escherichia coli, Megasphaera elsdenii, and anaerobic bacteria. By comparison, CTC-resistant bacteria in feral swine feces were over 1,000-fold fewer and exhibited lower taxonomic diversity. ...

journal_title：Applied and environmental microbiology

authors： Stanton TB,Humphrey SB,Stoffregen WC

更新日期：2011-10-01 00:00:00

abstract：:The bacterial rhizosphere communities of three host plants of the pathogenic fungus Verticillium dahliae, field-grown strawberry (Fragaria ananassa Duch.), oilseed rape (Brassica napus L.), and potato (Solanum tuberosum L.), were analyzed. We aimed to determine the degree to which the rhizosphere effect is plant depen...

journal_title：Applied and environmental microbiology

authors： Smalla K,Wieland G,Buchner A,Zock A,Parzy J,Kaiser S,Roskot N,Heuer H,Berg G

更新日期：2001-10-01 00:00:00

abstract：:Laying hens were individually caged at 20 weeks of age and tested for fecal excretion of Campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. Peak rates of C. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in October and in late April to early...

journal_title：Applied and environmental microbiology

authors： Doyle MP

更新日期：1984-03-01 00:00:00

abstract：:Because Bacteroides spp. are obligate anaerobes that dominate the human fecal flora, and because some species may live only in the human intestine, these bacteria might be useful to distinguish human from nonhuman sources of fecal pollution. To test this hypothesis, PCR primers specific for 16S rRNA gene sequences of ...

journal_title：Applied and environmental microbiology

authors： Kreader CA

更新日期：1995-04-01 00:00:00