当前位置： SCI文献检索 > APPLIED AND ENVIRONMENTAL MICROBIOLOGY期刊下所有文献 > Hydrogen production by the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under conditions of continuous light.

Hydrogen production by the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under conditions of continuous light.

Abstract：

:We report on the hydrogen production properties of the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142. This organism has a versatile metabolism and can grow in the presence or absence of combined nitrogen and can grow photosynthetically or mixotrophically and heterotrophically in the presence of glycerol. The strain produces a bidirectional hydrogenase (encoded by the hox genes), an uptake hydrogenase (hupLS), and nitrogenase (nifHDK). We demonstrated hydrogen production by both the hydrogenase and the nitrogenase under appropriate metabolic conditions. The highest rates of hydrogen production were produced under nitrogen-fixing conditions when cells were grown and incubated under continuous light conditions, in either the presence or absence of glycerol. Under such nitrogen-fixing conditions, we have achieved rates of 300 micromol H(2)/mg chloramphenicol (Chl)/hr during the first 24 h of incubation. The levels of H(2) measured were dependent upon the incubation conditions, such as sparging with argon, which generated anaerobic conditions. We demonstrated that the same conditions led to high levels of H(2) production and N(2) fixation, indicating that low-oxygen conditions favor nitrogenase activity for both processes. The levels of hydrogen produced by the hydrogenase are much lower, typically 5 to 10 micromol H(2)/mg Chl/hr. Hydrogenase activity was dependent upon electron transport through photosystem II (PS II), whereas nitrogenase activity was more dependent on PS I, as well as on respiration. Although cells do not double under the incubation conditions when sparged with argon to provide a low-oxygen environment, the cells are metabolically active, and hydrogen production can be inhibited by the addition of chloramphenicol to inhibit protein synthesis.

journal_name

Appl Environ Microbiol

journal_title

Applied and environmental microbiology

authors

Min H,Sherman LA

doi

10.1128/AEM.00146-10

subject

Has Abstract

pub_date

2010-07-01 00:00:00

pages

4293-301

issue

eissn

0099-2240

issn

1098-5336

pii

AEM.00146-10

journal_volume

pub_type

杂志文章

在线工具

Hydrogen production by the unicellular, diazotrophic cyanobacterium Cyanothece sp. strain ATCC 51142 under conditions of continuous light.