Repetitive, marker-free, site-specific integration as a novel tool for multiple chromosomal integration of DNA.

abstract：:Bacterial diversity in a deep-sea sediment was investigated by constructing actinobacterium-specific 16S ribosomal DNA (rDNA) clone libraries from sediment sections taken 5 to 12, 15 to 18, and 43 to 46 cm below the sea floor at a depth of 3,814 m. Clones were placed into operational taxonomic unit (OTU) groups with >...

journal_title：Applied and environmental microbiology

authors： Stach JE,Maldonado LA,Masson DG,Ward AC,Goodfellow M,Bull AT

更新日期：2003-10-01 00:00:00

abstract：:Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic r...

journal_title：Applied and environmental microbiology

authors： Scholz BK,Jakobek JL,Lindgren PB

更新日期：1994-04-01 00:00:00

abstract：:A bacterial strain, designated KSM-7358, that can use alpha-cedrene for growth was isolated. The strain was identified as a member of the genus Rhodococcus and catalyzed the novel allylic oxidation of alpha-cedrene regiospecifically to produce (R)-10-hydroxycedrene (sec-cedrenol) with a very high yield. alpha-Curcumen...

journal_title：Applied and environmental microbiology

authors： Takigawa H,Kubota H,Sonohara H,Okuda M,Tanaka S,Fujikura Y,Ito S

更新日期：1993-05-01 00:00:00

abstract：:Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

journal_title：Applied and environmental microbiology

authors： Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

更新日期：2006-06-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:Hydrolytic extracellular enzymes degrading host tissues potentially play a role in bacterial pathogenesis. Flavobacterium psychrophilum is an important bacterial pathogen of salmonid fish reared in freshwater throughout the world. Diversity among isolates has been described at the phenotypic, serological, and genomic ...

journal_title：Applied and environmental microbiology

authors： Rochat T,Pérez-Pascual D,Nilsen H,Carpentier M,Bridel S,Bernardet JF,Duchaud E

更新日期：2019-03-06 00:00:00

abstract：:Concerns regarding the depletion of the world's reserves of oil and global climate change have promoted an intensification of research and development toward the production of biofuels and other alternative sources of energy during the last years. There is currently much interest in developing the technology for third...

journal_title：Applied and environmental microbiology

authors： Ortiz-Marquez JC,Do Nascimento M,Dublan Mde L,Curatti L

更新日期：2012-04-01 00:00:00

abstract：:A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes. ...

journal_title：Applied and environmental microbiology

authors： Herman LM,De Block JH,Moermans RJ

更新日期：1995-02-01 00:00:00

abstract：:The comparative adherence of seven strains of Staphylococcus epidermidis to plastic surfaces was measured by an agar overlay technique in which adherent organisms were counted by their ability to form colonies under an agar overlay. The degree of adherence to plastics decreased in the order polystyrene, polyvinyl chlo...

journal_title：Applied and environmental microbiology

authors： Mackenzie AM,Rivera-Calderon RL

更新日期：1985-11-01 00:00:00

abstract：:Vegetative mycelia and spores of the investigated high- and low-producer strains of Streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. The release of antibiotic from the spores was easier when the spores were germinating. This phenomenon is conside...

journal_title：Applied and environmental microbiology

authors： Szabó I,Benedek A,Barabás G

更新日期：1985-08-01 00:00:00

abstract：:We report a method which combines epifluorescence microscopy and microautoradiography to determine both the total number of microorganisms in natural water populations and those individual organisms active in the uptake of specific substrates. After incubation with H-labeled substrate, the sample is filtered and, whil...

journal_title：Applied and environmental microbiology

authors： Tabor PS,Neihof RA

更新日期：1982-10-01 00:00:00

abstract：:Most of the edible mushrooms cannot be cultivated or have low yield under industrial conditions, partially due to the lack of knowledge on how basidioma (fruiting body) development is regulated. From winter mushroom (Flammulina velutipes), one of the most popular industrially cultivated mushrooms, a transcription fact...

journal_title：Applied and environmental microbiology

authors： Wu T,Hu C,Xie B,Zhang L,Yan S,Wang W,Tao Y,Li S

更新日期：2019-11-27 00:00:00

abstract：:Recent outbreaks of food-borne illness associated with the consumption of produce have increased concern over wildlife reservoirs of food-borne pathogens. Wild rodents are ubiquitous, and those living close to agricultural farms may pose a food safety risk should they shed zoonotic microorganisms in their feces near o...

journal_title：Applied and environmental microbiology

authors： Kilonzo C,Li X,Vivas EJ,Jay-Russell MT,Fernandez KL,Atwill ER

更新日期：2013-10-01 00:00:00

abstract：:Minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. These pathogens must attach to the produce in order to be transmitted. Cut surfaces of produce that expose cell walls are particularly vulnerable. Little is known about the roles that different structural...

journal_title：Applied and environmental microbiology

authors： Tan MS,Rahman S,Dykes GA

更新日期：2015-11-13 00:00:00

abstract：:Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

journal_title：Applied and environmental microbiology

authors： Elhanafi D,Dutta V,Kathariou S

更新日期：2010-12-01 00:00:00

abstract：:The lactose operon from Lactobacillus casei is regulated by very tight glucose repression and substrate induction mechanisms, which made it a tempting candidate system for the expression of foreign genes or metabolic engineering. An integrative vector was constructed, allowing stable gene insertion in the chromosomal ...

journal_title：Applied and environmental microbiology

authors： Gosalbes MJ,Esteban CD,Galán JL,Pérez-Martínez G

更新日期：2000-11-01 00:00:00

abstract：:Eleven strains of an undescribed species of Clavispora fermented D-xylose directly to ethanol under aerobic conditions. Strain UWO(PS)83-877-1 was grown in a medium containing 2% D-xylose and 0.5% yeast extract, and the following results were obtained: ethanol yield coefficient (ethanol/D-xylose), 0.29 g g (57.4% of t...

journal_title：Applied and environmental microbiology

authors： Nigam JN,Margaritis A,Lachance MA

更新日期：1985-10-01 00:00:00

abstract：:Clostridium thermohydrosulfuricum 39E produced a cell-bound cyclodextrin (CD)-degrading enzyme (cyclodextrinase). It was partially purified 205-fold (specific activity, 14.5 U/mg of protein) by solubilizing with Triton X-100, ammonium sulfate treatment, and DEAE-Sepharose CL-6B column chromatography. The enzyme activi...

journal_title：Applied and environmental microbiology

authors： Saha BC,Zeikus JG

更新日期：1990-09-01 00:00:00

abstract：:Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance of the plasmid. One is a minimum replicon including one gene, repT, coding a replication initiator, and the other encodes subunits of class I ribonucleotide redu...

journal_title：Applied and environmental microbiology

authors： Ohtani N,Tomita M,Itaya M

更新日期：2015-12-28 00:00:00

abstract：:The fate and impact of Pseudomonas aureofaciens TX-1 following application as a biocontrol agent for fungi in turfgrass were studied. The organism was applied with a modified irrigation system by using a preparation containing 1 x 10(6) P. aureofaciens TX-1 CFU ml(-1) about 100 times between May and August. We examine...

journal_title：Applied and environmental microbiology

authors： Sigler WV,Nakatsu CH,Reicher ZJ,Turco RF

更新日期：2001-08-01 00:00:00

abstract：:Sphingomonas sp. strain TTNP3 degrades 4(3',5'-dimethyl-3'-heptyl)-phenol and unidentified metabolites that were described previously. The chromatographic analyses of the synthesized reference compound and the metabolites led to their identification as 2(3',5'-dimethyl-3'-heptyl)-1,4-benzenediol. This finding indicate...

journal_title：Applied and environmental microbiology

authors： Corvini PF,Meesters RJ,Schäffer A,Schröder HF,Vinken R,Hollender J

更新日期：2004-11-01 00:00:00

abstract：:Entomopathogenic fungi can produce a series of chitinases, some of which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in insect fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified to homogeneity from liquid culture...

journal_title：Applied and environmental microbiology

authors： Fang W,Leng B,Xiao Y,Jin K,Ma J,Fan Y,Feng J,Yang X,Zhang Y,Pei Y

更新日期：2005-01-01 00:00:00

abstract：:An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

journal_title：Applied and environmental microbiology

authors： Hayashi M,Tabata K

更新日期：2013-05-01 00:00:00

abstract：:Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified...

journal_title：Applied and environmental microbiology

authors： Schoenborn L,Yates PS,Grinton BE,Hugenholtz P,Janssen PH

更新日期：2004-07-01 00:00:00

abstract：:The effects of silicic acid on the growth of Thermus thermophilus TMY, an extreme thermophile isolated from a siliceous deposit formed from geothermal water at a geothermal power plant in Japan, were examined at 75 degrees C. At concentrations higher than the solubility of amorphous silica (400 to 700 ppm SiO(2)), a s...

journal_title：Applied and environmental microbiology

authors： Doi K,Fujino Y,Inagaki F,Kawatsu R,Tahara M,Ohshima T,Okaue Y,Yokoyama T,Iwai S,Ogata S

更新日期：2009-04-01 00:00:00

abstract：:Preinfection development in Colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus Colletotrichum gloeosporioides f. sp. aeschynomene was shown to ...

journal_title：Applied and environmental microbiology

authors： Buhr TL,Dickman MB

更新日期：1997-06-01 00:00:00

abstract：:A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup...

journal_title：Applied and environmental microbiology

authors： De Beer D,Schramm A,Santegoeds CM,Kuhl M

更新日期：1997-03-01 00:00:00

abstract：:Bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2-hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). Pseudomonas cepacia P166 utilizes 4-chlorobiphenyl for growth and pro...

journal_title：Applied and environmental microbiology

authors： Arensdorf JJ,Focht DD

更新日期：1995-02-01 00:00:00

abstract：:A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

journal_title：Applied and environmental microbiology

authors： Tellenbach C,Grünig CR,Sieber TN

更新日期：2010-09-01 00:00:00

abstract：:The broad-host-range (BHR) conjugative plasmids have developed diverse adaptive mechanisms defining the range of their promiscuity. The BHR conjugative RA3 plasmid, the archetype of the IncU group, can transfer between, replicate in, and be maintained in representatives of Alpha-, Beta-, and Gammaproteobacteria Its st...

journal_title：Applied and environmental microbiology

authors： Lewicka E,Dolowy P,Godziszewska J,Litwin E,Ludwiczak M,Jagura-Burdzy G

更新日期：2020-08-03 00:00:00