Metabolic engineering for L-glutamine overproduction by using DNA gyrase mutations in Escherichia coli.

abstract：:A total of 25 marine caulobacters were isolated from littoral marine sources. Several aspects of their physiology and morphology were examined, as well as their suitability for genetic manipulation in laboratory cultivation. Caulobacters were readily isolated from all sources, including samples from areas containing p...

journal_title：Applied and environmental microbiology

authors： Anast N,Smit J

更新日期：1988-03-01 00:00:00

abstract：:A small spirillum, designated 5175, was isolated from an anaerobic enrichment culture for Desulfuromonas in which the major medium constituents were acetate and elemental sulfur. The organisms grew only under anaerobic or microaerophilic conditions. Elemental sulfur was formed anaerobically in a malate-sulfide medium,...

journal_title：Applied and environmental microbiology

authors： Wolfe RS,Penning N

更新日期：1977-02-01 00:00:00

abstract：:The ability of Streptococcus species to selectively adhere to the oral epithelial cells of newborns was studied in vitro. On day 1 of life, mucosal cells from normal infants demonstrated selective attraction for the natural distribution of streptococci that would soon colonize these surfaces. Streptococcus salivarius ...

journal_title：Applied and environmental microbiology

authors： Long SS,Swenson RM

更新日期：1976-10-01 00:00:00

abstract：:The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for ...

journal_title：Applied and environmental microbiology

authors： Dupray E,Cormier M

更新日期：1983-11-01 00:00:00

abstract：:Cultures of the newly isolated bacterial strains AD20, AD25, and AD27, identified as strains of Ancylobacter aquaticus, were capable of growth on 1,2-dichloroethane (DCE) as the sole carbon and energy source. These strains, as well as two other new DCE utilizers, were facultative methylotrophs and were also able to gr...

journal_title：Applied and environmental microbiology

authors： van den Wijngaard AJ,van der Kamp KW,van der Ploeg J,Pries F,Kazemier B,Janssen DB

更新日期：1992-03-01 00:00:00

abstract：:Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular parasite of aquatic amoebae and human macrophages. A key factor for L. pneumophila in intracellular infection is its type II protein secretion system (Lsp). In order to more completely define Lsp output, we recently performed a proteomic a...

journal_title：Applied and environmental microbiology

authors： Rossier O,Dao J,Cianciotto NP

更新日期：2008-02-01 00:00:00

abstract：:This report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, Bacillus anthracis. Bacillus cultures were prepared ...

journal_title：Applied and environmental microbiology

authors： Lasch P,Beyer W,Nattermann H,Stämmler M,Siegbrecht E,Grunow R,Naumann D

更新日期：2009-11-01 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:Glucose catabolite repression of tetralin catabolic genes in Rhodococcus sp. strain TFB was shown to be exerted by a protein homologous to transcriptional regulators of the cyclic AMP receptor (CRP)-FNR family. The protein was detected bound to putative CRP-like boxes localized at the promoters of the thnA1 and thnS g...

journal_title：Applied and environmental microbiology

authors： Tomás-Gallardo L,Santero E,Floriano B

更新日期：2012-08-01 00:00:00

abstract：:Vibrio alginolyticus is one of the most abundant microorganisms in marine environments and is also an opportunistic pathogen mediating high-mortality vibriosis in marine animals. Alternative sigma factors play essential roles in bacterial pathogens in the adaptation to environmental changes during infection and the ad...

journal_title：Applied and environmental microbiology

authors： Gu D,Zhang J,Hao Y,Xu R,Zhang Y,Ma Y,Wang Q

更新日期：2019-07-01 00:00:00

abstract：:Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

journal_title：Applied and environmental microbiology

authors： Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

更新日期：2003-02-01 00:00:00

abstract：:Two hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bact...

journal_title：Applied and environmental microbiology

authors： Tiwari SK,Sutyak Noll K,Cavera VL,Chikindas ML

更新日期：2015-03-01 00:00:00

abstract：:A total of 442 Listeria isolates, including 234 Listeria seeligeri, 80 L. monocytogenes, 74 L. welshimeri, 50 L. innocua, and 4 L. marthii isolates, were obtained from 1,805 soil, water, and other environmental samples collected over 2 years from four urban areas and four areas representing natural environments. Liste...

journal_title：Applied and environmental microbiology

authors： Sauders BD,Overdevest J,Fortes E,Windham K,Schukken Y,Lembo A,Wiedmann M

更新日期：2012-06-01 00:00:00

abstract：:Hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. Although these systems are common, little is known about the microbial populations that inhabit them or how...

journal_title：Applied and environmental microbiology

authors： McGarvey JA,Miller WG,Sanchez S,Stanker L

更新日期：2004-07-01 00:00:00

abstract：:Desulfitobacterium hafniense strain PCP-1 reductively dechlorinates pentachlorophenol (PCP) to 3-chlorophenol and a variety of halogenated aromatic compounds at the ortho, meta, and para positions. Several reductive dehalogenases (RDases) are thought to be involved in this cascade of dehalogenation. We partially purif...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Déziel E,Villemur R

更新日期：2010-11-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface ...

journal_title：Applied and environmental microbiology

authors： Johnson CN,Bowers JC,Griffitt KJ,Molina V,Clostio RW,Pei S,Laws E,Paranjpye RN,Strom MS,Chen A,Hasan NA,Huq A,Noriea NF 3rd,Grimes DJ,Colwell RR

更新日期：2012-10-01 00:00:00

abstract：:The growth kinetics of Flavobacterium sp. strain S12 specialized in the utilization of glycerol, and a number of oligo- and polysaccharides were determined in batch-culture experiments at 15 degrees C in pasteurized tap water supplied with very low amounts of substrates. Kss for the growth on maltotriose, maltotetraos...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Hijnen WA

更新日期：1985-04-01 00:00:00

abstract：:A monoclonal antibody designated 302 beta that is specific for the beta subunit of the oxygenase component (ISPTOL) of toluene dioxygenase from Pseudomonas putida F1 was used to prepare an immunoaffinity column. ISPTOL in cell extracts of Escherichia coli JM109(pDTG611) bound to the column, and an enzyme-linked immuno...

journal_title：Applied and environmental microbiology

authors： Lynch NA,Jiang H,Gibson DT

更新日期：1996-06-01 00:00:00

abstract：:Cryptosporidium is a genus of waterborne protozoan parasites that causes significant gastrointestinal disease in humans. These parasites can accumulate in environmental biofilms and be subsequently released to contaminate water supplies. Natural microbial assemblages were collected each season from an eastern Pennsylv...

journal_title：Applied and environmental microbiology

authors： Wolyniak EA,Hargreaves BR,Jellison KL

更新日期：2010-02-01 00:00:00

abstract：:Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical...

journal_title：Applied and environmental microbiology

authors： Monday SR,Keys C,Hanson P,Shen Y,Whittam TS,Feng P

更新日期：2006-04-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:Many Helicobacter pylori genetic studies would benefit from an ability to move DNA sequences easily between strains by transformation and homologous recombination, without needing to leave a conventional drug resistance determinant at the targeted locus. Presented here is a two-gene cassette that can be selected both ...

journal_title：Applied and environmental microbiology

authors： Dailidiene D,Dailide G,Kersulyte D,Berg DE

更新日期：2006-09-01 00:00:00

abstract：:In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica en...

journal_title：Applied and environmental microbiology

authors： Winkelmann N,Jaekel U,Meyer C,Serrano W,Rachel R,Rosselló-Mora R,Harder J

更新日期：2010-02-01 00:00:00

abstract：:A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reduc...

journal_title：Applied and environmental microbiology

authors： Nozawa-Inoue M,Jien M,Hamilton NS,Stewart V,Scow KM,Hristova KR

更新日期：2008-03-01 00:00:00

abstract：:Although the high level of competence for natural transformation of Acinetobacter sp. strain BD413 has been the subject of numerous studies, only two competence genes, comC and comP, have been identified to date. By chromosomal walking analysis we found two overlapping open reading frames, designated comE and comF, st...

journal_title：Applied and environmental microbiology

authors： Busch S,Rosenplänter C,Averhoff B

更新日期：1999-10-01 00:00:00

abstract：:PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4 degrees C in Dif...

journal_title：Applied and environmental microbiology

authors： Li X,Zhou X,Deng Z

更新日期：2004-07-01 00:00:00

abstract：:The cholera toxin genes of Vibrio cholerae are encoded by CTXΦ, a lysogenic bacteriophage. Infection with this phage plays a determinant role in toxigenicity conversion and the emergence of new clones of pathogenic V. cholerae. Multiple phage alleles, defined by sequence types of the repressor gene rstR, have been fou...

journal_title：Applied and environmental microbiology

authors： Wang H,Pang B,Xiong L,Wang D,Wang X,Zhang L,Kan B

更新日期：2015-10-01 00:00:00

abstract：:A model was constructed to simulate the results of experiments which investigated nitrification and denitrification in the freshwater sediment of Lake Vilhelmsborg, Denmark (K. Jensen, N. P. Sloth, N. Risgaard-Petersen, S. Rysgaard, and N. P. Revsbech, Appl. Environ. Microbiol. 60:2094-2100, 1994). The model output fa...

journal_title：Applied and environmental microbiology

authors： Blackburn TH,Blackburn ND,Jensen K,Risgaard-Petersen N

更新日期：1994-09-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

journal_title：Applied and environmental microbiology

authors： Nies L,Vogel TM

更新日期：1990-09-01 00:00:00