Abstract:
:Clostridium thermohydrosulfuricum 39E produced a cell-bound cyclodextrin (CD)-degrading enzyme (cyclodextrinase). It was partially purified 205-fold (specific activity, 14.5 U/mg of protein) by solubilizing with Triton X-100, ammonium sulfate treatment, and DEAE-Sepharose CL-6B column chromatography. The enzyme activity was found to be stable at pH 5.5 and 60 degrees C and optimally active at pH 6.0 and 65 degrees C. The enzyme preparation hydrolyzed CDs, with alpha-CD greater than beta-CD greater than gamma-CD, and displayed a putative multiple attack pattern. The enzyme activity was inhibited by p-chloromercuribenzoate but not by N-bromosuccinimide.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Saha BC,Zeikus JGdoi
10.1128/AEM.56.9.2941-2943.1990subject
Has Abstractpub_date
1990-09-01 00:00:00pages
2941-3issue
9eissn
0099-2240issn
1098-5336journal_volume
56pub_type
杂志文章abstract::7 alpha-Dehydroxylation of cholic acid and chenodeoxycholic acid by whole cells of strain c-25, a Eubacterium lentum-like intestinal anaerobe, was studied. 7 alpha-Dehydroxylase activity was observed only in whole cells grown in the presence of the primary bile acid (cholic acid or chenodeoxycholic acid). Chenodeoxych...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.4.735-739.1984
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doi:10.1128/AEM.00239-15
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.10.2394-2396.1987
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pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
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更新日期:2003-07-01 00:00:00
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更新日期:2012-04-01 00:00:00
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.51.1.65-73.1986
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journal_title:Applied and environmental microbiology
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更新日期:2012-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2011-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.6.1476-1478.1982
更新日期:1982-12-01 00:00:00