Production of glutaconic acid in a recombinant Escherichia coli strain.

abstract：:Pediocin PA-1 is an antimicrobial peptide (called bacteriocin) that shows inhibitory activity against the food-borne pathogen Listeria monocytogenes. To elucidate which residue(s) is responsible for this function, the antimicrobial activities of pediocin PA-1 mutants were evaluated and compared. Each of the 44 native ...

journal_title：Applied and environmental microbiology

authors： Tominaga T,Hatakeyama Y

更新日期：2006-02-01 00:00:00

abstract：:The production of biofuels from lignocellulose yields a substantial lignin by-product stream that currently has few applications. Biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to c...

journal_title：Applied and environmental microbiology

authors： Clarkson SM,Giannone RJ,Kridelbaugh DM,Elkins JG,Guss AM,Michener JK

更新日期：2017-08-31 00:00:00

abstract：:Aryl-alcohol oxidase (AAO) is an extracellular flavoprotein that supplies ligninolytic peroxidases with H2O2 during natural wood decay. With a broad substrate specificity and highly stereoselective reaction mechanism, AAO is an attractive candidate for studies into organic synthesis and synthetic biology, and yet the ...

journal_title：Applied and environmental microbiology

authors： Viña-Gonzalez J,Gonzalez-Perez D,Ferreira P,Martinez AT,Alcalde M

更新日期：2015-09-01 00:00:00

abstract：:Alfalfa sprouts and other seed sprouts have been implicated in numerous outbreaks of salmonellosis. The source of these epidemics appears to have been low-level contamination of seeds by Salmonella bacteria that developed into clinically significant populations during the seed germination process. To test the possibil...

journal_title：Applied and environmental microbiology

authors： Howard MB,Hutcheson SW

更新日期：2003-01-01 00:00:00

abstract：UNLABELLED:Lactobacillus rhamnosus GG is a lactic acid bacterium widely marketed by the food industry. Its genomic analysis led to the identification of a gene cluster encoding mucus-binding SpaCBA pili, which is located in a genomic island enriched in insertion sequence (IS) elements. In the present study, we analyzed...

journal_title：Applied and environmental microbiology

authors： Douillard FP,Ribbera A,Xiao K,Ritari J,Rasinkangas P,Paulin L,Palva A,Hao Y,de Vos WM

更新日期：2016-06-13 00:00:00

abstract：:In this study, the effect of individual lecithin phospholipids on the antimicrobial properties of eugenol against Escherichia coli C600 was investigated. We tested five major phospholipids common in soy or egg lecithin (1,2-dihexadecanoyl-sn-glycero-3-phosphocholine [DPPC], 1,2-dioctadecanoyl-sn-glycero-3-phosphocholi...

journal_title：Applied and environmental microbiology

authors： Zhang H,Dudley EG,Harte F

更新日期：2017-10-17 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests...

journal_title：Applied and environmental microbiology

authors： Tang M,Bideshi DK,Park HW,Federici BA

更新日期：2006-11-01 00:00:00

abstract：:Agromonas oligotrophica (Bradyrhizobium oligotrophicum) S58(T) is a nitrogen-fixing oligotrophic bacterium isolated from paddy field soil that is able to grow in extra-low-nutrient environments. Here, the complete genome sequence of S58 was determined. The S58 genome was found to comprise a circular chromosome of 8,26...

journal_title：Applied and environmental microbiology

authors： Okubo T,Fukushima S,Itakura M,Oshima K,Longtonglang A,Teaumroong N,Mitsui H,Hattori M,Hattori R,Hattori T,Minamisawa K

更新日期：2013-04-01 00:00:00

abstract：:Trehalose, a disaccharide accumulated by many microorganisms, acts as a protectant during periods of physiological stress, such as salinity and desiccation. Previous studies reported that the trehalose biosynthetic genes (otsA, treS, and treY) in Bradyrhizobium japonicum were induced by salinity and desiccation stress...

journal_title：Applied and environmental microbiology

authors： Sugawara M,Cytryn EJ,Sadowsky MJ

更新日期：2010-02-01 00:00:00

abstract：:Concentrations of T-2, HT-2, 3'-OH T-2, 3'-OH HT-2, T-2 triol, and T-2 tetraol toxins which inhibited [3H]thymidine uptake in mitogen-stimulated human peripheral lymphocytes by 50% were 1.5, 3.5, 4.0, 50, 150, and 150 ng/ml, respectively. The results suggested that the initial hydrolysis of T-2 toxin and the hydroxyla...

journal_title：Applied and environmental microbiology

authors： Forsell JH,Kateley JR,Yoshizawa T,Pestka JJ

更新日期：1985-06-01 00:00:00

abstract：:A straightforward method is described for screening methanotrophic colonies for soluble methane monooxygenase (sMMO) activity on solid media. Such activity results in the development of a colored complex between 1-naphthol, which is formed when sMMO reacts with naphthalene, and o-dianisidine (tetrazotized). Methanotro...

journal_title：Applied and environmental microbiology

authors： Graham DW,Korich DG,LeBlanc RP,Sinclair NA,Arnold RG

更新日期：1992-07-01 00:00:00

abstract：:The competition between selected or commercial killer strains of type K2 and sensitive commercial strains of Saccharomyces cerevisiae was studied under various conditions in sterile grape juice fermentations. The focus of this study was the effect of yeast inoculation levels and the role of assimilable nitrogen nutrit...

journal_title：Applied and environmental microbiology

authors： Medina K,Carrau FM,Gioia O,Bracesco N

更新日期：1997-07-01 00:00:00

abstract：:Two genes, accB and accE, that form part of the same operon, were cloned from Streptomyces coelicolor A3(2). AccB is homologous to the carboxyl transferase domain of several propionyl coezyme A (CoA) carboxylases and acyl-CoA carboxylases (ACCases) of actinomycete origin, while AccE shows no significant homology to an...

journal_title：Applied and environmental microbiology

authors： Rodríguez E,Banchio C,Diacovich L,Bibb MJ,Gramajo H

更新日期：2001-09-01 00:00:00

abstract：:Most probable numbers (MPNs) of methanogens in various salt marsh and estuarine sediments were determined with an anaerobic, habitat-simulating culture medium with 80% H(2) plus 20% CO(2) as substrate. Average MPNs for the short Spartina (SS) marsh sediments of Sapelo Island, Ga., were maximal at the 5- to 7-cm depth ...

journal_title：Applied and environmental microbiology

authors： Jones WJ,Paynter MJ

更新日期：1980-04-01 00:00:00

abstract：:Lactase (beta-d-galactosidase) was produced by Candida pseudotropicalis grown in deproteinized whey. Maximum enzyme production in 2% whey was obtained by supplementation with 0.15% yeast extract, 0.1% (NH(4))(2)SO(4), and 0.05% KH(2)PO(4) (wt/vol). Highest enzyme values (4.35 U/mg of cells and 68 U/ml) were obtained w...

journal_title：Applied and environmental microbiology

authors： de Bales SA,Castillo FJ

更新日期：1979-06-01 00:00:00

abstract：:Fresh produce is known to carry nonpathogenic epiphytic microorganisms. During agricultural production and harvesting, leafy greens can become contaminated with antibiotic-resistant pathogens or commensals from animal and human sources. As lettuce does not undergo any inactivation or preservation treatment during proc...

journal_title：Applied and environmental microbiology

authors： Holvoet K,Sampers I,Callens B,Dewulf J,Uyttendaele M

更新日期：2013-11-01 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:A screen of the Saccharomyces cerevisiae deletion strain set was performed to identify genes affecting hydrogen sulfide (H(2)S) production. Mutants were screened using two assays: colony color on BiGGY agar, which detects the basal level of sulfite reductase activity, and production of H(2)S in a synthetic juice mediu...

journal_title：Applied and environmental microbiology

authors： Linderholm AL,Findleton CL,Kumar G,Hong Y,Bisson LF

更新日期：2008-03-01 00:00:00

abstract：:The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and MonoQ-Sepharose. T...

journal_title：Applied and environmental microbiology

authors： Wanker E,Huber A,Schwab H

更新日期：1995-05-01 00:00:00

abstract：:Norwalk virus and other human caliciviruses (noroviruses) are major agents of gastroenteritis, and water is a major route of their transmission. In an effort to control Norwalk virus in drinking water, Norwalk virus reduction by bench-scale ozone disinfection was determined using quantitative reverse transcription (RT...

journal_title：Applied and environmental microbiology

authors： Shin GA,Sobsey MD

更新日期：2003-07-01 00:00:00

abstract：:The presence of bacteria in salted anchovies during and at the end of the curing process was investigated. Attempts to isolate bacteria under aerobic or anaerobic conditions led to the isolation of only bacteria of the genus Pediococcus which were identified as Pediococcus halophilus. The isolates correspond to a rath...

journal_title：Applied and environmental microbiology

authors： Villar M,de Ruiz Holgado AP,Sanchez JJ,Trucco RE,Oliver G

更新日期：1985-03-01 00:00:00

abstract：:Recent functional genomics and genome-scale modeling approaches indicated that B(12) production in Lactobacillus reuteri could be improved by optimization of the medium. Here we show that a series of systematic single-amino-acid omissions could significantly modulate the production of B(12) from nearly undetectable le...

journal_title：Applied and environmental microbiology

authors： Santos F,Teusink B,Molenaar D,van Heck M,Wels M,Sieuwerts S,de Vos WM,Hugenholtz J

更新日期：2009-06-01 00:00:00

abstract：:The colonization of granular activated carbon columns by bacteria can have both beneficial and potentially detrimental consequences. Bacterial growth on the carbon surface can remove adsorbed organics and thus partially regenerate the carbon bed. However, growth can also increase the levels of bacteria in the column e...

journal_title：Applied and environmental microbiology

authors： Shimp RJ,Pfaender FK

更新日期：1982-08-01 00:00:00

abstract：:Yeasts able to grow on d-xylose were screened for the ability to hydrolyze xylan. Xylanase activity was found to be rare; a total of only 19 of more than 250 strains yielded a positive test result. The activity was localized largely in the genus Cryptococcus and in Pichia stipitis and its anamorph Candida shehatae. Th...

journal_title：Applied and environmental microbiology

authors： Lee H,Biely P,Latta RK,Barbosa MF,Schneider H

更新日期：1986-08-01 00:00:00

abstract：:The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...

journal_title：Applied and environmental microbiology

authors： Aranda-Olmedo I,Marín P,Ramos JL,Marqués S

更新日期：2006-11-01 00:00:00

abstract：:We developed a PCR detection method that selectively recognizes a single biological control agent and demonstrated that universally primed PCR (UP-PCR) can identify strain-specific markers. Antagonistic strains of Clonostachys rosea (syn. Gliocladium roseum) were screened by UP-PCR, and a strain-specific marker was id...

journal_title：Applied and environmental microbiology

authors： Bulat SA,Lübeck M,Alekhina IA,Jensen DF,Knudsen IM,Lübeck PS

更新日期：2000-11-01 00:00:00

abstract：:Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount...

journal_title：Applied and environmental microbiology

authors： Akeboshi H,Chiba Y,Kasahara Y,Takashiba M,Takaoka Y,Ohsawa M,Tajima Y,Kawashima I,Tsuji D,Itoh K,Sakuraba H,Jigami Y

更新日期：2007-08-01 00:00:00

abstract：:Lactic and acetic acids were determined to be slightly synergistic in their inhibitory interrelationship against Salmonella typhimurium with the use of a modified toxicological model. ...

journal_title：Applied and environmental microbiology

authors： Rubin HE

更新日期：1978-10-01 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00