Construction and Optimization of a Heterologous Pathway for Protocatechuate Catabolism in Escherichia coli Enables Bioconversion of Model Aromatic Compounds.

abstract：:Previously, we successfully cloned a d-cycloserine (d-CS) biosynthetic gene cluster consisting of 10 open reading frames (designated dcsA to dcsJ) from d-CS-producing Streptomyces lavendulae ATCC 11924. In this study, we put four d-CS biosynthetic genes (dcsC, dcsD, dcsE, and dcsG) in tandem under the control of the T...

journal_title：Applied and environmental microbiology

authors： Kumagai T,Ozawa T,Tanimoto M,Noda M,Matoba Y,Sugiyama M

更新日期：2015-11-01 00:00:00

abstract：:A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup...

journal_title：Applied and environmental microbiology

authors： De Beer D,Schramm A,Santegoeds CM,Kuhl M

更新日期：1997-03-01 00:00:00

abstract：UNLABELLED:We previously isolated the transaminase KES23458 from Pseudomonas sp. strain AAC as a promising biocatalyst for the production of 12-aminododecanoic acid, a constituent building block of nylon-12. Here, we report the subsequent characterization of this transaminase. It exhibits activity with a broad substrat...

journal_title：Applied and environmental microbiology

authors： Wilding M,Peat TS,Newman J,Scott C

更新日期：2016-06-13 00:00:00

abstract：:Magnetotactic bacteria comprise a phylogenetically diverse group that is capable of synthesizing intracellular magnetic particles. Although various morphotypes of magnetotactic bacteria have been observed in the environment, bacterial strains available in pure culture are currently limited to a few genera due to diffi...

journal_title：Applied and environmental microbiology

authors： Arakaki A,Shibusawa M,Hosokawa M,Matsunaga T

更新日期：2010-03-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...

journal_title：Applied and environmental microbiology

authors： Gill AO,Holley RA

更新日期：2004-10-01 00:00:00

abstract：:Escherichia coli KO11 was previously constructed to produce ethanol from acid hydrolysates of hemicellulose (pentoses and hexoses) by the chromosomal integration of Zymomonas mobilis genes encoding pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adhB). Klebsiella oxytoca P2 was constructed in an analogous fash...

journal_title：Applied and environmental microbiology

authors： Moniruzzaman M,Lai X,York SW,Ingram LO

更新日期：1997-12-01 00:00:00

abstract：:Hot and cold aqueous extracts were prepared from 22 commonly ingested fruits, vegetables, and seeds. When tested by agar diffusion, extracts from 13 and 10 of the foods formed precipitin bands with samples of normal rabbit serum and human saliva, respectively; extracts from four of the foods also reacted with antigen ...

journal_title：Applied and environmental microbiology

authors： Gibbons RJ,Dankers I

更新日期：1981-04-01 00:00:00

abstract：:Yarrowia lipolytica produces brown extracellular pigments that correlate with tyrosine catabolism. During tyrosine depletion, the yeast accumulated homogentisic acid, p-hydroxyphenylethanol, and p-hydroxyphenylacetic acid in the medium. Homogentisic acid accumulated under all aeration conditions tested, but its concen...

journal_title：Applied and environmental microbiology

authors： Carreira A,Ferreira LM,Loureiro V

更新日期：2001-08-01 00:00:00

abstract：:Algal-bacterial mats which grow in the effluent channels of alkaline hot springs provided an environment suitable for studying natural thermophilic methane producing bacteria. Methane was rapidly produced in cores taken from the meat and appeared to be an end product of decomposition of the algal-bacterial organic mat...

journal_title：Applied and environmental microbiology

authors： Ward DM

更新日期：1978-06-01 00:00:00

abstract：:Citrus canker, caused by Xanthomonas citri pv. citri, is a serious disease of citrus plants worldwide. Earlier phylogenetic studies using housekeeping genes revealed that X. citri pv. citri is related to many other pathovars, which can be collectively referred as Xanthomonas citri pathovars (XCPs). From the present st...

journal_title：Applied and environmental microbiology

authors： Bansal K,Midha S,Kumar S,Patil PB

更新日期：2017-04-17 00:00:00

abstract：:A mathematical model was developed to estimate the number of logarithmic cycles (LDec) of lipopolysaccharide concentration destroyed by a dry-heat sterilization process. The LDec values calculated from the mathematical model agreed well with those obtained from the destruction of lipopolysaccharide by a dry-heat treat...

journal_title：Applied and environmental microbiology

authors： Tsuji K,Lewis AR

更新日期：1978-11-01 00:00:00

abstract：:Although their exact function remains enigmatic, bifidobacteria are among the first colonizers of the newborn infant gut and further develop into abundant communities, notably in response to diet. Therefore, the transcriptional responses of bifidobacteria in rapidly processed fecal samples from young infants that were...

journal_title：Applied and environmental microbiology

authors： Klaassens ES,Boesten RJ,Haarman M,Knol J,Schuren FH,Vaughan EE,de Vos WM

更新日期：2009-05-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00

abstract：:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-so...

journal_title：Applied and environmental microbiology

authors： Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

更新日期：1992-08-01 00:00:00

abstract：:Strain VeGlc2, an anaerobic ultramicrobacterium belonging to the Verrucomicrobiales lineage of bacterial descent, fermented glucose to acetate, propionate, succinate, and CO2. The distribution of radiolabel in the fermentation end products produced from position-labelled glucose and in vitro measurements of enzyme act...

journal_title：Applied and environmental microbiology

authors： Janssen PH

更新日期：1998-12-01 00:00:00

abstract：:Epimerization of sugar nucleotides is central to the structural diversification of monosaccharide building blocks for cellular biosynthesis. Epimerase applicability to carbohydrate synthesis can be limited, however, by the high degree of substrate specificity exhibited by most sugar nucleotide epimerases. Here, we dis...

journal_title：Applied and environmental microbiology

authors： Rapp C,van Overtveldt S,Beerens K,Weber H,Desmet T,Nidetzky B

更新日期：2020-12-04 00:00:00

abstract：:Perchlorate is a known health hazard for humans, fish, and other species. Therefore, it is important to assess the response of an ecosystem exposed to perchlorate contamination. The data reported here show that a liquid chromatography-mass spectrometry-based proteomics approach for the detection of perchlorate-reducin...

journal_title：Applied and environmental microbiology

authors： Bansal R,Crawford RL,Paszczynski AJ

更新日期：2011-02-01 00:00:00

abstract：:Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscil...

journal_title：Applied and environmental microbiology

authors： Hallez R,Letesson JJ,Vandenhaute J,De Bolle X

更新日期：2007-02-01 00:00:00

abstract：:Volume 61, no. 12, p. 4505, Introduction, column 1, line 13, and column 2, line 5: reference 19, which refers to a toxicity test based on the inhibition of (beta)-glucuronidase, not (beta)-galactosidase, in E. coli, should not be cited. [This corrects the article on p. 4505 in vol. 61.]. ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1996-02-01 00:00:00

abstract：:After the first isolation of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in 2003, this MRSA variant quickly became the predominant MRSA obtained from humans as part of the Dutch national MRSA surveillance. Previous studies have suggested that human-to-human transmission of LA-MRSA, compa...

journal_title：Applied and environmental microbiology

authors： Bosch T,Verkade E,van Luit M,Landman F,Kluytmans J,Schouls LM

更新日期：2015-01-01 00:00:00

abstract：:A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

journal_title：Applied and environmental microbiology

authors： Visscher PT,Taylor BF

更新日期：1994-12-01 00:00:00

abstract：:Spent sulfite liquor (SSL) is a waste effluent from sulfite pulping that contains monomeric sugars which can be fermented to ethanol. However, fermentative yeasts used for the fermentation of the sugars in SSL are adversely affected by the inhibitory substances in this complex feedstock. To overcome this limitation, e...

journal_title：Applied and environmental microbiology

authors： Pinel D,D'Aoust F,del Cardayre SB,Bajwa PK,Lee H,Martin VJ

更新日期：2011-07-01 00:00:00

abstract：:The consumption of fresh tomatoes has been linked to numerous food-borne outbreaks involving various serovars of Salmonella enterica. Recent advances in our understanding of plant-microbe interactions have shown that human enteric pathogenic bacteria, including S. enterica, are adapted to survive in the plant environm...

journal_title：Applied and environmental microbiology

authors： Zheng J,Allard S,Reynolds S,Millner P,Arce G,Blodgett RJ,Brown EW

更新日期：2013-04-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic r...

journal_title：Applied and environmental microbiology

authors： Scholz BK,Jakobek JL,Lindgren PB

更新日期：1994-04-01 00:00:00

abstract：:The interactive effect of phosphate-solubilizing bacteria and arbuscular mycorrhizal (AM) fungi on plant use of soil P sources of low bioavailability (endogenous or added as rock phosphate [RP] material) was evaluated by using soil microcosms which integrated (sup32)P isotopic dilution techniques. The microbial inocul...

journal_title：Applied and environmental microbiology

authors： Toro M,Azcon R,Barea J

更新日期：1997-11-01 00:00:00

abstract：:An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

journal_title：Applied and environmental microbiology

authors： Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

更新日期：2018-05-17 00:00:00

abstract：:There is a growing interest in the use of microbial fermentation for the generation of high-demand, high-purity chemicals using cheap feedstocks in an environmentally friendly manner. One example explored here is the production of isoprene (C5H8), a hemiterpene, which is primarily polymerized to polyisoprene in synthe...

journal_title：Applied and environmental microbiology

authors： Diner BA,Fan J,Scotcher MC,Wells DH,Whited GM

更新日期：2017-12-15 00:00:00

abstract：:The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...

journal_title：Applied and environmental microbiology

authors： Alvarez AJ,Buttner MP,Stetzenbach LD

更新日期：1995-10-01 00:00:00