Novel Bacillus thuringiensis insecticidal crystal protein with a silent activity against coleopteran larvae.

Abstract:

:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-solubilized crystals are toxic to any of several Lepidoptera and Coleoptera species tested. In contrast, a trypsin-treated solution containing the 72-kDa tryptic core fragment of the protoxin is highly toxic to Colorado potato beetle larvae. The crystal protein-encoding gene was cloned and sequenced. The inferred amino acid sequence of the putative toxic fragment has 37, 32, and 33% homology to the CryIIIA, CryIIIB, and CryIIID toxins, respectively. Interestingly, the 501 C-terminal amino acids show 41 to 48% amino acid identity with corresponding C-terminal amino acid sequences of other crystal proteins. Because of the toxicity of the fragment to the Colorado potato beetle and because of the distinct similarities of the toxic fragment with the other CryIII proteins, this gene was given a new subclass name (cryIIIC) within the CryIII class of coleopteran-active crystal proteins. CryIIIC represents the first example of a crystal protein with a silent activity towards coleopteran insect larvae. Natural CryIIIC crystals are not toxic. Toxicity is revealed only after an in vitro solubilization and activation step.

journal_name

Appl Environ Microbiol

authors

Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

doi

10.1128/AEM.58.8.2536-2542.1992

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

2536-42

issue

8

eissn

0099-2240

issn

1098-5336

journal_volume

58

pub_type

杂志文章
  • Spoilage of chicken skin at 2 degrees C: electron microscopic study.

    abstract::Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.2.492-503.1981

    authors: Thomas CJ,McMeekin TA

    更新日期:1981-02-01 00:00:00

  • Transmission of Escherichia coli O157:H7 from contaminated manure and irrigation water to lettuce plant tissue and its subsequent internalization.

    abstract::The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.1.397-400.2002

    authors: Solomon EB,Yaron S,Matthews KR

    更新日期:2002-01-01 00:00:00

  • Demethylation of dimethylsulfoniopropionate to 3-mercaptopropionate by an aerobic marine bacterium.

    abstract::A bacterium, strain BIS-6, that grew aerobically on dimethylsulfoniopropionate (DMSP) was isolated from an intertidal mud sample. Strain BIS-6 quantitatively demethylated DMSP and 3-methiolpropionate to 3-mercaptopropionate. Strain BIS-6 was a versatile methylotroph growing on the osmolytes DMSP and glycine betaine an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.12.4617-4619.1994

    authors: Visscher PT,Taylor BF

    更新日期:1994-12-01 00:00:00

  • Comparison of Pulsed-Field Gel Electrophoresis DNA Fingerprints of Field Isolates of the Entomopathogen Bacillus popilliae.

    abstract::Eight strains of the entomopathogen Bacillus popilliae were analyzed by pulsed-field gel electrophoresis, and genomic size estimates of ;sim2,600 to 3,500 kb were obtained. The type strain, ATCC 14706, had a genomic size of 3,395 kb. For the six New Zealand isolates, the degree of similarity in the pulsed-field gel el...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.6.2446-2449.1995

    authors: Macdonald R,Kalmakoff J

    更新日期:1995-06-01 00:00:00

  • Absence of livestock-associated methicillin-resistant staphylococcus aureus clonal complex CC398 as a nasal colonizer of pigs raised in an alternative system.

    abstract::Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 isolated from pigs raised in conventional farms was previously reported. Here we report a study on 25 farms adhering to an alternative system. LA-MRSA ST398 was not detected in nasal swabs from 178 pigs or from 89 humans working and livin...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07260-11

    authors: Cuny C,Friedrich AW,Witte W

    更新日期:2012-02-01 00:00:00

  • Association of azospirillum with grass roots.

    abstract::The association between grass roots and Azospirillum brasilense Sp 7 was investigated by the Fahraeus slide technique, using nitrogen-free medium. Young inoculated roots of pearl millet and guinea grass produced more mucilaginous sheath (mucigel), root hairs, and lateral roots than did uninoculated sterile controls. T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.1.219-226.1980

    authors: Umali-Garcia M,Hubbell DH,Gaskins MH,Dazzo FB

    更新日期:1980-01-01 00:00:00

  • Transformation of Dibenzo-p-Dioxin by Pseudomonas sp. Strain HH69.

    abstract::Dibenzo-p-dioxin was oxidatively cleaved by the dibenzofuran-degrading bacterium Pseudomonas sp. strain HH69 to produce minor amounts of 1-hydroxydibenzo-p-dioxin and catechol, while a 2-phenoxy derivative of muconic acid was formed as the major product. Upon acidic methylation, the latter yielded the dimethylester of...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.1157-1159.1990

    authors: Harms H,Wittich RM,Sinnwell V,Meyer H,Fortnagel P,Francke W

    更新日期:1990-04-01 00:00:00

  • Improved specimen coating technique for scanning electron microscope observation of decomposer microorganisms.

    abstract::Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.2.313-315.1976

    authors: Draggan S

    更新日期:1976-02-01 00:00:00

  • Response of Vibrio cholerae to Low-Temperature Shifts: CspV Regulation of Type VI Secretion, Biofilm Formation, and Association with Zooplankton.

    abstract:UNLABELLED:The ability to sense and adapt to temperature fluctuation is critical to the aquatic survival, transmission, and infectivity of Vibrio cholerae, the causative agent of the disease cholera. Little information is available on the physiological changes that occur when V. cholerae experiences temperature shifts....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00807-16

    authors: Townsley L,Sison Mangus MP,Mehic S,Yildiz FH

    更新日期:2016-06-30 00:00:00

  • The weak acid preservative sorbic acid inhibits conidial germination and mycelial growth of Aspergillus niger through intracellular acidification.

    abstract::The growth of the filamentous fungus Aspergillus niger, a common food spoilage organism, is inhibited by the weak acid preservative sorbic acid (trans-trans-2,4-hexadienoic acid). Conidia inoculated at 10(5)/ml of medium showed a sorbic acid MIC of 4.5 mM at pH 4.0, whereas the MIC for the amount of mycelia at 24 h de...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.6.3506-3511.2004

    authors: Plumridge A,Hesse SJ,Watson AJ,Lowe KC,Stratford M,Archer DB

    更新日期:2004-06-01 00:00:00

  • Fecal bacteroidales diversity and decay in response to variations in temperature and salinity.

    abstract::Bacteroidales are attractive as water quality indicators because of their potential to discern sources of fecal pollution, and it is presumed that these bacteria do not multiply outside their host organisms. The persistence of a fecal Bacteroidales marker was monitored over 14 days in river water microcosms that varie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01473-10

    authors: Schulz CJ,Childers GW

    更新日期:2011-04-01 00:00:00

  • Involvement of plasmids in total degradation of chlorinated biphenyls.

    abstract::Acinetobacter sp. strain P6 has previously been reported to utilize biphenyl (BP) and chlorinated BPs, with accumulation of corresponding chlorobenzoic acids. Arthrobacter sp. strain M5 was isolated as a contaminant in the culture of Acinetobacter sp. strain P6 growing on 4-chlorobiphenyl and showed properties similar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.3.619-626.1982

    authors: Furukawa K,Chakrabarty AM

    更新日期:1982-09-01 00:00:00

  • Isolation and characterization of an anaerobic ruminal bacterium capable of degrading hydrolyzable tannins.

    abstract::An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic aci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.9.3293-3298.1995

    authors: Nelson KE,Pell AN,Schofield P,Zinder S

    更新日期:1995-09-01 00:00:00

  • Phylogenetic ecology of the freshwater Actinobacteria acI lineage.

    abstract::The acI lineage of freshwater Actinobacteria is a cosmopolitan and often numerically dominant member of lake bacterial communities. We conducted a survey of acI 16S rRNA genes and 16S-23S rRNA internal transcribed spacer regions from 18 Wisconsin lakes and used standard nonphylogenetic and phylogenetic statistical app...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00794-07

    authors: Newton RJ,Jones SE,Helmus MR,McMahon KD

    更新日期:2007-11-01 00:00:00

  • Single-Cell Protein Production by the Acid-Tolerant Fungus Scytalidium acidophilum from Acid Hydrolysates of Waste Paper.

    abstract::The bioconversion of waste paper to single-cell protein at pH <1 by Scytalidium acidophilum is described. Waste paper pretreated with 72% H(2)SO(4) at 4 degrees C was diluted with water to a pH of <0.1 and hydrolyzed. This yielded an adequate sugar-containing substrate for the growth of the fungus. A total of 97% of t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.3.643-647.1982

    authors: Ivarson KC,Morita H

    更新日期:1982-03-01 00:00:00

  • Relationship between nitrogen-fixing sulfate reducers and fermenters in salt marsh sediments and roots of Spartina alterniflora.

    abstract::A combination of inhibitors and carbon substrates was used to determine the relative contribution of sulfate-reducing bacteria (SRB) and fermenting bacteria to nitrogen fixation in a salt marsh sediment and on the roots of Spartina alterniflora. Because a lag period precedes acetylene-reducing activity (ARA) in amende...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.8.2031-2036.1988

    authors: Gandy EL,Yoch DC

    更新日期:1988-08-01 00:00:00

  • VIRTUS, a model of virus transport in unsaturated soils.

    abstract::As a result of the recently proposed mandatory groundwater disinfection requirements to inactivate viruses in potable water supplies, there has been increasing interest in virus fate and transport in the subsurface. Several models have been developed to predict the fate of viruses in groundwater, but few include trans...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1609-1616.1992

    authors: Yates MV,Ouyang Y

    更新日期:1992-05-01 00:00:00

  • Fermented Milk Containing Lactobacillus casei Strain Shirota Preserves the Diversity of the Gut Microbiota and Relieves Abdominal Dysfunction in Healthy Medical Students Exposed to Academic Stress.

    abstract:UNLABELLED:Stress-induced abdominal dysfunction is an attractive target for probiotics. To investigate the effects of the probiotic Lactobacillus casei strain Shirota on abdominal dysfunction, a double-blind, placebo-controlled trial was conducted with healthy medical students undertaking an authorized nationwide exami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,随机对照试验

    doi:10.1128/AEM.04134-15

    authors: Kato-Kataoka A,Nishida K,Takada M,Kawai M,Kikuchi-Hayakawa H,Suda K,Ishikawa H,Gondo Y,Shimizu K,Matsuki T,Kushiro A,Hoshi R,Watanabe O,Igarashi T,Miyazaki K,Kuwano Y,Rokutan K

    更新日期:2016-05-31 00:00:00

  • Cold-active serine alkaline protease from the psychrotrophic bacterium Shewanella strain ac10: gene cloning and enzyme purification and characterization.

    abstract::The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of 85,113. SapSh exhib...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.2.611-617.1999

    authors: Kulakova L,Galkin A,Kurihara T,Yoshimura T,Esaki N

    更新日期:1999-02-01 00:00:00

  • Comparative Genotypic and Phenotypic Analysis of Cronobacter Species Cultured from Four Powdered Infant Formula Production Facilities: Indication of Pathoadaptation along the Food Chain.

    abstract::Cronobacter species are opportunistic pathogens commonly found in the environment. Among the seven Cronobacter species, Cronobacter sakazakii sequence type 4 (ST-4) is predominantly associated with recorded cases of infantile meningitis. This study reports on a 26-month powdered infant formula (PIF) surveillance progr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00359-15

    authors: Yan Q,Wang J,Gangiredla J,Cao Y,Martins M,Gopinath GR,Stephan R,Lampel K,Tall BD,Fanning S

    更新日期:2015-07-01 00:00:00

  • Lecithin agar for detection of microbial phospholipases.

    abstract::Lecithin agar was developed on which phospholipase C produced turbid zones and phospholipase A produced clear zones. Reactions on lecithin agar agreed 74% of the time with reactions in egg yolk broth. On lecithin agar, interpretation was easier, phospholipase A was detectable, and opaque zones were visible 1 or 2 days...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.31.5.784-786.1976

    authors: Chrisope GL,Fox CW,Marshall RT

    更新日期:1976-05-01 00:00:00

  • Enteric Viruses in Surface Waters from Argentina: Molecular and Viable-Virus Detection.

    abstract::Water resources contaminated with wastewater are an important source for the dissemination of enteric viruses with an impact on the health of the population. The aim of the study was to assess the viral contamination of freshwater from a dam in Argentina by using infectious enterovirus detection, viral RNA amplificati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02327-17

    authors: Masachessi G,Pisano MB,Prez VE,Martínez LC,Michelena JF,Martínez-Wassaf M,Giordano MO,Isa MB,Pavan JV,Welter A,Nates SV,Ré V

    更新日期:2018-02-14 00:00:00

  • Characterization and role of the branched-chain aminotransferase (BcaT) isolated from Lactococcus lactis subsp. cremoris NCDO 763.

    abstract::In Lactococcus lactis, which is widely used as a starter in the cheese industry, the first step of aromatic and branched-chain amino acid degradation is a transamination which is catalyzed by two major aminotransferases. We have previously purified and characterized biochemically and genetically the aromatic aminotran...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.2.571-577.2000

    authors: Yvon M,Chambellon E,Bolotin A,Roudot-Algaron F

    更新日期:2000-02-01 00:00:00

  • Lead inhibition of enzyme synthesis in soil.

    abstract::Addition of 2 mg of Pb2+/g of soil concident with or after amendment with starch or maltose resulted in 75 and 50% decreases in net synthesis of amylase and alpha-glucosidase, respectively. Invertase synthesis in sucrose-amended soil was transiently reduced after Pb2+ addition. Amylase activity was several times less ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.2.262-268.1977

    authors: Cole MA

    更新日期:1977-02-01 00:00:00

  • Stability of the delta-endotoxin gene from Bacillus thuringiensis subsp. kurstaki in a recombinant strain of Clavibacter xyli subsp. cynodontis.

    abstract::Deletion of chromosomally inserted gene sequences from Clavibacter xyli subsp. cynodontis, a xylem-inhabiting endophyte, was studied in vitro and in planta. We found that nonreplicating plasmid pCG610, which conferred resistance to kanamycin and tetracycline and contained segments of C. xyli subsp. cynodontis genomic ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.12.3522-3528.1991

    authors: Turner JT,Lampel JS,Stearman RS,Sundin GW,Gunyuzlu P,Anderson JJ

    更新日期:1991-12-01 00:00:00

  • Viability of lyophilized cyanobacteria (blue-green algae).

    abstract::Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.6.777-780.1976

    authors: Corbett LL,Parker DL

    更新日期:1976-12-01 00:00:00

  • Effect of light on the cell cycle of a marine synechococcus strain.

    abstract::Light-dependent regulation of cell cycle progression in the marine cyanobacterium Synechococcus strain WH-8101 was demonstrated through the use of flow cytometry. Our results show that, similar to eucaryotic cells, marine Synechococcus spp. display two gaps in DNA synthesis, at the beginning and at the end of the cell...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.2.425-432.1989

    authors: Armbrust EV,Bowen JD,Olson RJ,Chisholm SW

    更新日期:1989-02-01 00:00:00

  • Enhancement of varicella-zoster virus replication in cultured human embryonic lung cells treated with the pesticide carbaryl.

    abstract::In studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (VZ) virus-infected human embryonic lung cells with the pesticide carbaryl. The replication of the cell-associated VZ virus was enhanced 2- to 13-fold as c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.3.652-656.1981

    authors: Abrahamsen LH,Jerkofsky M

    更新日期:1981-03-01 00:00:00

  • Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii.

    abstract::Improving access to the carbohydrate content of lignocellulose is key to reducing recalcitrance for microbial deconstruction and conversion to fuels and chemicals. Caldicellulosiruptor bescii completely solubilizes naked microcrystalline cellulose, yet this transformation is impeded within the context of the plant cel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00969-17

    authors: Zurawski JV,Khatibi PA,Akinosho HO,Straub CT,Compton SH,Conway JM,Lee LL,Ragauskas AJ,Davison BH,Adams MWW,Kelly RM

    更新日期:2017-08-17 00:00:00

  • Effect of hydrophobicity of utilization of peptides by ruminal bacteria in vitro.

    abstract::When mixed ruminal bacteria were incubated with a pancreatic casein hydrolysate and free amino acids of a similar composition, rates of ammonia production were much greater for peptides than for amino acids. The pancreatic digest of casein was then fractionated with 90% isopropyl alcohol. Hydrophobic peptides which di...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.9.2021-2025.1987

    authors: Chen G,Strobel HJ,Russell JB,Sniffen CJ

    更新日期:1987-09-01 00:00:00