Abstract:
:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-solubilized crystals are toxic to any of several Lepidoptera and Coleoptera species tested. In contrast, a trypsin-treated solution containing the 72-kDa tryptic core fragment of the protoxin is highly toxic to Colorado potato beetle larvae. The crystal protein-encoding gene was cloned and sequenced. The inferred amino acid sequence of the putative toxic fragment has 37, 32, and 33% homology to the CryIIIA, CryIIIB, and CryIIID toxins, respectively. Interestingly, the 501 C-terminal amino acids show 41 to 48% amino acid identity with corresponding C-terminal amino acid sequences of other crystal proteins. Because of the toxicity of the fragment to the Colorado potato beetle and because of the distinct similarities of the toxic fragment with the other CryIII proteins, this gene was given a new subclass name (cryIIIC) within the CryIII class of coleopteran-active crystal proteins. CryIIIC represents the first example of a crystal protein with a silent activity towards coleopteran insect larvae. Natural CryIIIC crystals are not toxic. Toxicity is revealed only after an in vitro solubilization and activation step.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen Mdoi
10.1128/AEM.58.8.2536-2542.1992keywords:
subject
Has Abstractpub_date
1992-08-01 00:00:00pages
2536-42issue
8eissn
0099-2240issn
1098-5336journal_volume
58pub_type
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1995-09-01 00:00:00
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更新日期:1982-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.54.8.2031-2036.1988
更新日期:1988-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-05-01 00:00:00
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pub_type: 杂志文章,随机对照试验
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pub_type: 杂志文章
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更新日期:1976-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2018-02-14 00:00:00
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pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1977-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.32.6.777-780.1976
更新日期:1976-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.3.652-656.1981
更新日期:1981-03-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2017-08-17 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.9.2021-2025.1987
更新日期:1987-09-01 00:00:00