Abstract:
:The association between grass roots and Azospirillum brasilense Sp 7 was investigated by the Fahraeus slide technique, using nitrogen-free medium. Young inoculated roots of pearl millet and guinea grass produced more mucilaginous sheath (mucigel), root hairs, and lateral roots than did uninoculated sterile controls. The bacteria were found within the mucigel that accumulated on the root cap and along the root axes. Adherent bacteria were associated with granular material on root hairs and fibrillar material on undifferentiated epidermal cells. Significantly fewer numbers of azospirilla attached to millet root hairs when the roots were grown in culture medium supplemented with 5 mM potassium nitrate. Under these growth conditions, bacterial attachment to undifferentiated epidermal cells was unaffected. Aseptically collected root exudate from pearl millet contained substances which bound to azospirilla and promoted their adsorption to the root hairs. This activity was associated with nondialyzable and proteasesensitive substances in root exudate. Millet root hairs adsorbed azospirilla in significantly higher numbers than cells of Rhizobium, Pseudomonas, Azotobacter, Klebsiella, or Escherichia. Pectolytic activities, including pectin transeliminase and endopolygalacturonase, were detected in pure cultures of A. brasilense when this species was grown in a medium containing pectin. These studies describe colonization of grass root surfaces by A. brasilense and provide a possible explanation for the limited colonization of intercellular spaces of the outer root cortex.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Umali-Garcia M,Hubbell DH,Gaskins MH,Dazzo FBdoi
10.1128/AEM.39.1.219-226.1980keywords:
subject
Has Abstractpub_date
1980-01-01 00:00:00pages
219-26issue
1eissn
0099-2240issn
1098-5336journal_volume
39pub_type
杂志文章abstract::High concentrations of free metal ions in the environment can be detrimental to bacterial survival. However, bacteria utilize strategies, including the activation of stress response pathways and immobilizing chemical elements on their surface, to limit this toxicity. In this study, we characterized LA4131, the HtpX-li...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01097-13
更新日期:2013-08-01 00:00:00
abstract::Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth....
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.4.951-953.1986
更新日期:1986-10-01 00:00:00
abstract::An extracellular protein with strong absorption at 406 nm was purified from cell-free culture fluid of latex-grown Xanthomonas sp. strain 35Y. This protein was identical to the gene product of a recently characterized gene cloned from Xanthomonas sp., as revealed by determination of m/z values and sequencing of select...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.12.7388-7395.2004
更新日期:2004-12-01 00:00:00
abstract::Although only partially understood, multicellular behavior is relatively common in bacterial pathogens. Bacterial aggregates can resist various host defenses and colonize their environment more efficiently than planktonic cells. For the waterborne pathogen Legionella pneumophila, little is known about the roles of aut...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03254-13
更新日期:2014-02-01 00:00:00
abstract::Norovirus is the leading cause of food-related illness in the United States, and contamination of ready-to-eat items by food handlers poses a high risk for disease. This study reports the in vitro (suspension test) and in vivo (fingerpad protocol) assessments of a new ethanol-based hand sanitizer containing a synergis...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00487-08
更新日期:2008-08-01 00:00:00
abstract::Microcosms capable of reductive dechlorination of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) were constructed in glass bottles by seeding them with a polluted river sediment and incubating them anaerobically with an organic medium. All of the PCDD/F congeners detected were equally reduced without the ac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.8.4325-4334.2005
更新日期:2005-08-01 00:00:00
abstract::We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.3.683-687.1988
更新日期:1988-03-01 00:00:00
abstract::In contrast to extensive studies of phosphorus, widely considered the main nutrient limiting phytoplankton biomass in freshwater ecosystems, there have been few studies on the role of nitrogen in controlling phytoplankton populations. This situation may be due partly to the complexity in estimating its utilization and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.3.1465-1474.2003
更新日期:2003-03-01 00:00:00
abstract::Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of se...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02238-07
更新日期:2008-03-01 00:00:00
abstract::Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and A189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. Each PCR primer set w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.9.3802-3809.2001
更新日期:2001-09-01 00:00:00
abstract::Recently, a new metabolic link between fatty acid de novo biosynthesis and biosynthesis of poly(3-hydroxy-alkanoate) consisting of medium-chain-length constituents (C(6) to C(14)) (PHA(MCL)), catalyzed by the 3-hydroxydecanoyl-[acyl-carrier-protein]:CoA transacylase (PhaG), has been identified in Pseudomonas putida (B...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.5.2117-2124.2000
更新日期:2000-05-01 00:00:00
abstract::Arhodomonas sp. strain Seminole was isolated from a crude oil-impacted brine soil and shown to degrade benzene, toluene, phenol, 4-hydroxybenzoic acid (4-HBA), protocatechuic acid (PCA), and phenylacetic acid (PAA) as the sole sources of carbon at high salinity. Seminole is a member of the genus Arhodomonas in the cla...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01509-14
更新日期:2014-11-01 00:00:00
abstract::We report the identification of novel archaea living in close association with bacteria in the cold (approximately 10 degrees C) sulfurous marsh water of the Sippenauer Moor near Regensburg, Bavaria, Germany. These microorganisms form a characteristic, macroscopically visible structure, morphologically comparable to a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.5.2336-2344.2001
更新日期:2001-05-01 00:00:00
abstract::The unusual compound beta-aminoglutaric acid (beta-glutamate) has been identified by 13C nuclear magnetic resonance spectroscopy in soluble extracts of marine methanogenic bacteria. We examined several methanogen species representing nine genera and found that beta-glutamate occurred in methanococci and two methanogen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.5.1504-1508.1990
更新日期:1990-05-01 00:00:00
abstract::The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.3.880-887.1994
更新日期:1994-03-01 00:00:00
abstract::Ari1p from Saccharomyces cerevisiae, recently identified as an intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains fu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00542-10
更新日期:2010-08-01 00:00:00
abstract::Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.8.1996-2002.1988
更新日期:1988-08-01 00:00:00
abstract::Filamentous, gliding, sulfide-oxidizing bacteria of the genus Thioploca were found on sediments in profundal areas of Lake Biwa, a Japanese freshwater mesotrophic lake, and were characterized morphologically and phylogenetically. The Lake Biwa Thioploca resembled morphologically Thioploca ingrica, a brackish water spe...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.1.390-398.2003
更新日期:2003-01-01 00:00:00
abstract::Many but not all ammonia-oxidizing bacteria (AOB) produce urease (urea amidohydrolase, EC 3.5.1.5) and are capable of using urea for chemolithotrophic growth. We sequenced the urease operons from two AOB, the beta-proteobacterium Nitrosospira sp. strain NpAV and the gamma-proteobacterium Nitrosococcus oceani. In both ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.4.2342-2348.2004
更新日期:2004-04-01 00:00:00
abstract::The pathways for degradation of aromatic hydrocarbons are constantly modified by a variety of genetic mechanisms. Genetic studies carried out with Pseudomonas stutzeri OX1 suggested that the tou operon coding for toluene o-xylene monooxygenase (ToMO) was recently recruited into a preexisting pathway that already posse...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.8.4736-4743.2005
更新日期:2005-08-01 00:00:00
abstract::A novel strain of Halomonas (Tc-202), which has the capability of removing Tc(VII) from solid- and aqueous-phase material aerobically, was isolated from the marine environment. Tc-202 removed 55% of the total 99Tc in solutions at 15 degrees C by reducing Tc(VII) to Tc(V), but other Halomonas strains did not. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00819-06
更新日期:2006-12-01 00:00:00
abstract::Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.7.2104-2108.1991
更新日期:1991-07-01 00:00:00
abstract::We examined the bacterial communities of epilithic biofilms in 17 streams which represented a gradient ranging from relatively pristine streams to streams highly impacted by acid mine drainage (AMD). A combination of automated ribosomal intergenic spacer analysis with multivariate analysis and ordination provided a se...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00274-09
更新日期:2009-06-01 00:00:00
abstract::The deposition of manganese in a water distribution system with manganese-related "dirty water" problems was studied over a 1-year period. Four monitoring laboratories with Robbins biofilm sampling devices fitted to the water mains were used to correlate the relationship among manganese deposition, the level of mangan...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.3.628-639.1990
更新日期:1990-03-01 00:00:00
abstract::Microorganisms associated with the stems and roots of nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans [Glycine max (L.) Merril] were analyzed by ribosomal intergenic transcribed spacer analysis (RISA) and automated RISA (ARISA). RISA of stem samples detected no bands specific...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00833-08
更新日期:2008-09-01 00:00:00
abstract::Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03765-15
更新日期:2015-12-18 00:00:00
abstract::A new detection system, the magnetic immuno-polymerase chain reaction (PCR) assay (MIPA) has been developed to detect Listeria monocytogenes in food. This method separates Listeria cells from PCR-inhibitory factors present in enrichment broths containing food samples by using magnetic beads coated with specific monocl...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.5.1289-1293.1993
更新日期:1993-05-01 00:00:00
abstract::A method is described for generating and screening variants of the beta-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus sensitive to several environmental stresses, with potential application in the food industry. Chemical mutagenesis with hydroxylamine or methoxylamine was performed on the beta-galacto...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.4.1221-1226.1994
更新日期:1994-04-01 00:00:00
abstract::The addition of positively charged colloidal materials to the growth medium markedly increased the concentration of cellulase enzymes produced by Trichoderma reesei Rut-C30. Filter paper activities of up to 4 and 13 IU/ml have been achieved by the addition of colloidal materials, using 3% lactose and 3% cellulose, res...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.4.934-938.1985
更新日期:1985-04-01 00:00:00
abstract::A homokaryotic, clavine alkaloid-producing strain of ergot, Claviceps sp. strain SD 58, was used in an attempt to demonstrate parasexuality. Genetically marked auxotrophic strains were produced by mutation with N-methyl-N'-nitro-N-nitrosoguanidine. Protoplast fusion of pairs of unlike doubly auxotrophic strains and is...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.1.70-73.1987
更新日期:1987-01-01 00:00:00