Comparison of pmoA PCR primer sets as tools for investigating methanotroph diversity in three Danish soils.

abstract：:A protein (PhaX) that interacted with poly(3-hydroxybutyrate) (PHB) depolymerase PhaZa1 and with PHB granule-associated phasin protein PhaP2 was identified by two-hybrid analysis. Deletion of phaX resulted in an increase in the level of polyphosphate (polyP) granule formation and in impairment of PHB utilization in nu...

journal_title：Applied and environmental microbiology

authors： Tumlirsch T,Sznajder A,Jendrossek D

更新日期：2015-12-01 00:00:00

abstract：:The genotyping of numerous isolates of Cryptosporidium parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (GP60) has been favored by ...

journal_title：Applied and environmental microbiology

authors： Widmer G,Lee Y

更新日期：2010-10-01 00:00:00

abstract：:A latent virus-like agent, which we designated zooxanthella filamentous virus 1 (ZFV1), was isolated from Symbiodinium sp. strain CCMP 2465 and characterized. Transmission electron microscopy and analytical flow cytometry revealed the presence of a new group of distinctive filamentous virus-like particles after exposu...

journal_title：Applied and environmental microbiology

authors： Lohr J,Munn CB,Wilson WH

更新日期：2007-05-01 00:00:00

abstract：:Gamma irradiation is a nonthermal processing technology that has been used for the preservation of a variety of food products. This technology has been shown to effectively inactivate bacterial pathogens. Currently, the FDA has approved doses of up to 4.0 kGy to control food-borne pathogens in fresh iceberg lettuce an...

journal_title：Applied and environmental microbiology

authors： Feng K,Divers E,Ma Y,Li J

更新日期：2011-05-01 00:00:00

abstract：:Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms in...

journal_title：Applied and environmental microbiology

authors： Wang IK,Lai HC,Yu CJ,Liang CC,Chang CT,Kuo HL,Yang YF,Lin CC,Lin HH,Liu YL,Chang YC,Wu YY,Chen CH,Li CY,Chuang FR,Huang CC,Lin CH,Lin HC

更新日期：2012-02-01 00:00:00

abstract：:The phosphotransferase system regulation domain (PRD)-containing activator, ManR, is required for glucose-controlled transcription of the mannose permease two (mpt) operon in Listeria innocua. His-871 in ManR PRD-II is needed for mpt repression in glucose-free media. His-506 in PRD-I is needed for mpt induction by glu...

journal_title：Applied and environmental microbiology

authors： Xue J,Miller KW

更新日期：2007-09-01 00:00:00

abstract：:When 7-aminocephalosporanic acid (7-ACA) was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms, pink yeast colonies appeared frequently, and these were identified as Rhodotorula glutinis. These intact R. glutinis cells converted (i) 7-ACA to deacetyl-7-ACA (7-...

journal_title：Applied and environmental microbiology

authors： Sakai Y,Abe T,Ohbayashi Y,Isaka K,Yamamoto K,Tani Y,Kato N

更新日期：1996-07-01 00:00:00

abstract：:Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to un...

journal_title：Applied and environmental microbiology

authors： Fischer M,Sawers RG

更新日期：2013-07-01 00:00:00

abstract：:The degradation of three polycyclic aromatic hydrocarbons (PAH), pyrene (PYR), benz[a]anthracene (BAA), and benzo[a]pyrene (BaP), by Mycobacterium sp. strain RJGII-135 was studied. The bacterium was isolated from an abandoned coal gasification site soil by analog enrichment techniques and found to mineralize [14C]PYR....

journal_title：Applied and environmental microbiology

authors： Schneider J,Grosser R,Jayasimhulu K,Xue W,Warshawsky D

更新日期：1996-01-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:The plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination with the gene for nitrate reductase (niaD) as the selectable marker. The frequency of cotransformation, as determined by GUS expression on plates conta...

journal_title：Applied and environmental microbiology

authors： Couteaudier Y,Daboussi MJ,Eparvier A,Langin T,Orcival J

更新日期：1993-06-01 00:00:00

abstract：:In May 1999, field surveys of Lyme disease spirochetes were conducted around the Tianshan Mountains in Xinjiang Uygur Autonomous Region in northwestern People's Republic of China. Ixodes persulcatus ticks were obtained in a Tianchi Lake valley with primary forest, while the tick fauna was poor in the semidesert or at ...

journal_title：Applied and environmental microbiology

authors： Takada N,Masuzawa T,Ishiguro F,Fujita H,Kudeken M,Mitani H,Fukunaga M,Tsuchiya K,Yano Y,Ma XH

更新日期：2001-11-01 00:00:00

abstract：:Atypical El Tor strains of Vibrio cholerae O1 harboring variant ctxB genes of cholera toxin (CT) have gradually become a major cause of recent cholera epidemics. Vibrio mimicus occasionally produces CT, encoded by ctxAB on CTXФ genome; toxin-coregulated pilus (TCP), a major intestinal colonization factor; and also the...

journal_title：Applied and environmental microbiology

authors： Neogi SB,Chowdhury N,Awasthi SP,Asakura M,Okuno K,Mahmud ZH,Islam MS,Hinenoya A,Nair GB,Yamasaki S

更新日期：2019-01-23 00:00:00

abstract：:Nineteen Tn5-induced mutants of Rhizobium fredii HH303 defective in acidic exopolysaccharide synthesis were isolated by screening for lack of Calcofluor fluorescence. They were grouped by complementation analysis by using Rhizobium meliloti cosmids carrying exo genes. All of the 19 mutants were symbiotically effective...

journal_title：Applied and environmental microbiology

authors： Kim CH,Tully RE,Keister DL

更新日期：1989-07-01 00:00:00

abstract：:Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is str...

journal_title：Applied and environmental microbiology

authors： Giebler J,Wick LY,Schloter M,Harms H,Chatzinotas A

更新日期：2013-05-01 00:00:00

abstract：:Epilithic bacteria were isolated nonselectively from riverbed stones and examined by gel zymography for their ability to produce alkylsulfatase (AS) enzymes and thus to metabolize alkyl sulfate surfactants such as sodium dodecyl sulfate. The percentages of AS+ isolates from stone epilithon at five sites from the sourc...

journal_title：Applied and environmental microbiology

authors： Anderson DJ,Day MJ,Russell NJ,White GF

更新日期：1988-02-01 00:00:00

abstract：:A proteinaceous substance with antibiotic-like activity, resembling that of a bacteriocin, was isolated from an industrial-scale acetone-butanol fermentation of Clostridium acetobutylicum. The substance, purified by acetone precipitation, diethylaminoethyl cellulose chromatography, and polyacrylamide gel electrophores...

journal_title：Applied and environmental microbiology

authors： Webster JR,Reid SJ,Jones DT,Woods DR

更新日期：1981-02-01 00:00:00

abstract：:The concentration of 2-aminoethylphosphonic acid has been measured in seven genera of rumen ciliate protozoa. Expressed as milligrams per gram of total nitrogen, 2-aminoethylphosphonic acid concentrations ranged from 17.2 in Ophryoscolex spp. to 72.4 in Eremoplastron spp. ...

journal_title：Applied and environmental microbiology

authors： Whitelaw FG,Bruce LA,Eadie JM,Shand WJ

更新日期：1983-10-01 00:00:00

abstract：:Nontoxigenic strains of Clostridium botulinum types C and D are converted to toxigenic strains by infection with specific Tox+ bacteriophages. The nucleic acids were extracted from five converting phages, c-st, c-468, c-203, c-d6f, and d-1873, and one nonconverting phage, c-n71, and treated with nucleases. The nucleic...

journal_title：Applied and environmental microbiology

authors： Fujii N,Oguma K,Yokosawa N,Kimura K,Tsuzuki K

更新日期：1988-01-01 00:00:00

abstract：:The phospholipid fatty acid (PLFA) pattern was analyzed in a forest humus and in an arable soil experimentally polluted with Cd, Cu, Ni, Pb, or Zn at different concentrations. In both soil types, there were gradual changes in the PLFA patterns for the different levels of metal contamination. The changes in the forest ...

journal_title：Applied and environmental microbiology

authors： Frostegård A,Tunlid A,Bååth E

更新日期：1993-11-01 00:00:00

abstract：:Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did...

journal_title：Applied and environmental microbiology

authors： Cooper JE,Bjourson AJ,Thompson JK

更新日期：1987-07-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Bacterial source tracking is used to apportion fecal pollution among putative sources. Within this context, library-independent markers are genetic or phenotypic traits that can be used to identify the host origin without a need for library-dependent classification functions. The objective of this project was to use m...

journal_title：Applied and environmental microbiology

authors： Soule M,Kuhn E,Loge F,Gay J,Call DR

更新日期：2006-03-01 00:00:00

abstract：:To simulate growth conditions experienced by microbiota at O(inf2)-limited interfaces of organic matter in compost, an experimental system capable of maintaining dual limitations of oxygen and carbon for extended periods, i.e., a pO(inf2)-auxostat, has been used. (sup15)N tracer studies on thermophilic (53(deg)C) deco...

journal_title：Applied and environmental microbiology

authors： Pel R,Oldenhuis R,Brand W,Vos A,Gottschal JC,Zwart KB

更新日期：1997-02-01 00:00:00

abstract：:[This corrects the article on p. 1743 in vol. 64, PMID: 9572946.]. ...

journal_title：Applied and environmental microbiology

authors： Kaucner C,Stinear T

更新日期：1998-11-01 00:00:00

abstract：:By taking advantage of MazF, an ACA codon-specific mRNA interferase, Escherichia coli cells can be converted into a bioreactor producing only a single protein of interest by using an ACA-less mRNA for the protein. In this single-protein production (SPP) system, we engineered MazF by replacing two tryptophan residues i...

journal_title：Applied and environmental microbiology

authors： Vaiphei ST,Mao L,Shimazu T,Park JH,Inouye M

更新日期：2010-09-01 00:00:00

abstract：:We have developed a program that can accurately analyze the dynamic properties of tethered bacterial cells. The program works especially well with cells that tend to give rise to unstable rotations, such as polar-flagellated bacteria. The program has two novel components. The first dynamically adjusts the center of th...

journal_title：Applied and environmental microbiology

authors： Qian C,Wong CC,Swarup S,Chiam KH

更新日期：2013-08-01 00:00:00

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), ...

journal_title：Applied and environmental microbiology

authors： Unnerstad H,Nilsson I,Ericsson H,Danielsson-Tham ML,Bille J,Bannerman E,Tham W

更新日期：1999-05-01 00:00:00

abstract：:The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...

journal_title：Applied and environmental microbiology

authors： Marciano-Cabral FM,Fulford DE

更新日期：1986-05-01 00:00:00