SOD1, a new Kluyveromyces lactis helper gene for heterologous protein secretion.

abstract：:Iron (Fe) is one of the most important micronutrients for most life forms on earth. While abundant in soil, Fe bioavailability in oxic soil is very low. Under environmental conditions, bacteria need to acquire sufficient Fe to sustain growth while limiting the energy cost of siderophore synthesis. Biofilm formation mi...

journal_title：Applied and environmental microbiology

authors： Rizzi A,Leroux J,Charron-Lamoureux V,Roy S,Beauregard PB,Bellenger JP

更新日期：2020-10-28 00:00:00

abstract：:Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

journal_title：Applied and environmental microbiology

authors： Zhou K,George SM,Métris A,Li PL,Baranyi J

更新日期：2011-03-01 00:00:00

abstract：:We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture. ...

journal_title：Applied and environmental microbiology

authors： Hirata Y,Minami J,Koyama M,Matsushita O,Katayama S,Jin F,Maeta H,Okabe A

更新日期：1995-11-01 00:00:00

abstract：:Ethane oxidation was studied in ethane-grown resting cells (mycelia) of an Acremonium sp. and in cell-free preparations of such mycelia. From resting cell experiments evidence was found for a pathway of ethane oxidation via ethanol, acetaldehyde, and acetic acid. In vitro studies indicated that ethane-oxidizing activi...

journal_title：Applied and environmental microbiology

authors： Davies JS,Wellman AM,Zajic JE

更新日期：1976-07-01 00:00:00

abstract：:The abundance of quorum quenching (QQ) activity was evaluated in cultivable bacteria obtained from oceanic and estuarine seawater and compared with the frequency of QQ enzyme sequences in the available marine metagenomic collections. The possible role of the high QQ activity found among marine bacteria is discussed. ...

journal_title：Applied and environmental microbiology

authors： Romero M,Martin-Cuadrado AB,Otero A

更新日期：2012-09-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a...

journal_title：Applied and environmental microbiology

authors： Huq A,Colwell RR,Rahman R,Ali A,Chowdhury MA,Parveen S,Sack DA,Russek-Cohen E

更新日期：1990-08-01 00:00:00

abstract：:Agrochemicals, fecal indicator bacteria (FIB), and pathogens frequently contaminate water simultaneously. No significant direct effects of fertilizer, atrazine, malathion, and chlorothalonil on the survival of Escherichia coli, Enterococcus faecalis, Salmonella enterica, human polyomaviruses, and adenovirus were detec...

journal_title：Applied and environmental microbiology

authors： Staley ZR,Senkbeil JK,Rohr JR,Harwood VJ

更新日期：2012-11-01 00:00:00

abstract：:Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

journal_title：Applied and environmental microbiology

authors： Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

更新日期：2007-10-01 00:00:00

abstract：:The differential resistance of substratum-attached, detached, and planktonic cells of Salmonella enteritidis phage type 4 was studied by using several inimical processes and in vivo bioluminescence as a nondestructive, real-time reporter of metabolic activity. Bioluminescence in this strain was mediated by a construct...

journal_title：Applied and environmental microbiology

authors： Dhir VK,Dodd CE

更新日期：1995-05-01 00:00:00

abstract：:The thermophilic homoacetogenic bacterium Moorella sp. strain HUC22-1 ferments glyoxylate to acetate roughly according to the reaction 2 glyoxylate --> acetate + 2 CO(2). A batch culture with glyoxylate and yeast extract yielded 11.7 g per mol of cells per substrate, which was much higher than that obtained with H(2) ...

journal_title：Applied and environmental microbiology

authors： Sakai S,Inokuma K,Nakashimada Y,Nishio N

更新日期：2008-03-01 00:00:00

abstract：:One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...

journal_title：Applied and environmental microbiology

authors： Gonella E,Negri I,Marzorati M,Mandrioli M,Sacchi L,Pajoro M,Crotti E,Rizzi A,Clementi E,Tedeschi R,Bandi C,Alma A,Daffonchio D

更新日期：2011-02-01 00:00:00

abstract：:Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...

journal_title：Applied and environmental microbiology

authors： Fuchs SW,Jaskolla TW,Bochmann S,Kötter P,Wichelhaus T,Karas M,Stein T,Entian KD

更新日期：2011-03-01 00:00:00

abstract：:Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong an...

journal_title：Applied and environmental microbiology

authors： Zhang L,Sun C

更新日期：2018-08-31 00:00:00

abstract：:A selective procedure was used to isolate pectinolytic intestinal bacteria from human subjects. The three isolates with the greatest pectinolytic activity utilized pectin and a few related compounds as fermentable substrates for growth but did not utilize any other compound tested. Thus, their substrate utilization pa...

journal_title：Applied and environmental microbiology

authors： Jensen NS,Canale-Parola E

更新日期：1985-07-01 00:00:00

abstract：:Streptococcus thermophilus is one of the most widely used lactic acid bacteria in the dairy industry, in particular in yoghurt manufacture, where it is associated with Lactobacillus delbrueckii subsp. bulgaricus. This bacterial association, known as a proto-cooperation, is poorly documented at the molecular and regula...

journal_title：Applied and environmental microbiology

authors： Herve-Jimenez L,Guillouard I,Guedon E,Boudebbouze S,Hols P,Monnet V,Maguin E,Rul F

更新日期：2009-04-01 00:00:00

abstract：:The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...

journal_title：Applied and environmental microbiology

authors： Martinez-Medina M,Garcia-Gil J,Barnich N,Wieler LH,Ewers C

更新日期：2011-08-15 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:Bacterial communities associated with marine algae are often dominated by members of the Roseobacter clade, and in the present study, we describe Roseobacter phenotypes that may provide this group of bacteria with selective advantages when colonizing this niche. Nine of 14 members of the Roseobacter clade, of which ha...

journal_title：Applied and environmental microbiology

authors： Bruhn JB,Gram L,Belas R

更新日期：2007-01-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:Two Baeyer-Villiger monooxygenases (BVMOs), designated BoBVMO and AmBVMO, were discovered from Bradyrhizobium oligotrophicum and Aeromicrobium marinum, respectively. Both monooxygenases displayed novel features for catalyzing the asymmetric sulfoxidation of bulky and pharmaceutically relevant thioethers. Evolutionary ...

journal_title：Applied and environmental microbiology

authors： Zhang Y,Liu F,Xu N,Wu YQ,Zheng YC,Zhao Q,Lin G,Yu HL,Xu JH

更新日期：2018-07-02 00:00:00

abstract：:Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE,Rodrick GE

更新日期：1981-02-01 00:00:00

abstract：:The kinetics of concentration-dependent toluene metabolism were examined by evaluating each term in the second-order rate equation. Marine and freshwater pseudomonads were used. Uptake for Pseudomonas sp. strain T2 was characterized by a completely saturatable system with small transport constant (K(t) = 44 mug/liter)...

journal_title：Applied and environmental microbiology

authors： Robertson BR,Button DK

更新日期：1987-09-01 00:00:00

abstract：:Iron is an essential micronutrient for life. In mammals, dietary iron is absorbed primarily in the small intestine. Currently, the impacts of dietary iron on the taxonomic structure and function of the gut microbiome and reciprocal effects on the animal host are not well understood. Here, we establish a mouse model of...

journal_title：Applied and environmental microbiology

authors： Coe GL,Pinkham NV,Celis AI,Johnson C,DuBois JL,Walk ST

更新日期：2021-01-15 00:00:00

abstract：:The N-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent I) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. The study included concentration, contact time, pH, temperature, and water quality as controlled variables. The species of bacteria teste...

journal_title：Applied and environmental microbiology

authors： Williams DE,Worley SD,Wheatley WB,Swango LJ

更新日期：1985-03-01 00:00:00

abstract：:Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields an...

journal_title：Applied and environmental microbiology

authors： Larraya LM,Alfonso M,Pisabarro AG,Ramírez L

更新日期：2003-06-01 00:00:00

abstract：:A radiometric method for the detection of Salmonella in foods has been developed which is based on Salmonella poly H agglutinating serum preventing Salmonella from producing 14CO2 from [14C]dulcitol. The method will detect the presence or absence of Salmonella in a product within 30 h compared to 4 to 5 days by routin...

journal_title：Applied and environmental microbiology

authors： Stewart BJ,Eyles MJ,Murrell WG

更新日期：1980-08-01 00:00:00

abstract：:We analyzed the regional distribution of bulk heterotrophic prokaryotic activity (leucine incorporation) and selected single-cell parameters (cell viability and nucleic acid content) as parameters for microbial functioning, as well as bacterial and archaeal community structure in the epipelagic (0 to 200 m) and mesope...

journal_title：Applied and environmental microbiology

authors： Baltar F,Arístegui J,Gasol JM,Herndl GJ

更新日期：2012-05-01 00:00:00

abstract：:Populations of feral pigs (Sus scrofa) may serve as an environmental reservoir of Cryptosporidium parvum oocysts and Giardia sp. cysts for source water. We conducted a cross-sectional study to determine the prevalence of and associated demographic and environmental risk factors for the shedding of C. parvum oocysts an...

journal_title：Applied and environmental microbiology

authors： Atwill ER,Sweitzer RA,Pereira MG,Gardner IA,Van Vuren D,Boyce WM

更新日期：1997-10-01 00:00:00

abstract：:Multiple anthropogenic disturbances to bacterial diversity have been investigated in coastal ecosystems, in which temporal variability in the bacterioplankton community has been considered a ubiquitous process. However, far less is known about the temporal dynamics of a bacterioplankton community responding to polluti...

journal_title：Applied and environmental microbiology

authors： Wang K,Zhang D,Xiong J,Chen X,Zheng J,Hu C,Yang Y,Zhu J

更新日期：2015-01-01 00:00:00