Abstract:
:Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields and productivities. Traditionally, fungal breeding has been carried out by an empirical trial and error process. In this study, we used a different approach by mapping quantitative trait loci (QTLs) controlling culture production and quality within the framework of the genetic linkage map of P. ostreatus. Ten production traits and four quality traits were studied and mapped. The production QTLs identified explain nearly one-half of the production variation. More interestingly, a single QTL mapping to the highly polymorphic chromosome VII appears to be involved in control of all the productivity traits studied. Quality QTLs appear to be scattered across the genome and to have less effect on the variation of the corresponding traits. Moreover, some of the new hybrid strains constructed in the course of our experiments had production or quality values higher than those of the parents or other commercial strains. This approach opens the possibility of marker-assisted selection and breeding of new industrial strains of this fungus.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Larraya LM,Alfonso M,Pisabarro AG,Ramírez Ldoi
10.1128/aem.69.6.3617-3625.2003keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
3617-25issue
6eissn
0099-2240issn
1098-5336journal_volume
69pub_type
杂志文章abstract::For PCR detection of Coxiella burnetii in various clinical specimens we developed a sample preparation method in which silica binding of DNA was used. This method was found to be fast, easily performed with large numbers of samples, and equally sensitive for all of the specimens tested (livers, spleens, placentas, hea...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.11.4234-4237.1998
更新日期:1998-11-01 00:00:00
abstract::A total of 286 isolates of Pseudomonas maltophilia was collected from sheep exhibiting brown or yellowish fleece rot and from fly-strike lesions. Enzyme activities for 10 of the isolates were examined by plate tests and with the API ZYM system and compared with the enzymatic profile of a human type strain of P. maltop...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.51.2.346-348.1986
更新日期:1986-02-01 00:00:00
abstract::The strain Burkholderia cepacia G4 aerobically mineralized trichloroethene (TCE) to CO(2) over a time period of approximately 20 h. Three biodegradation experiments were conducted with different bacterial optical densities at 540 nm (OD(540)s) in order to test whether isotope fractionation was consistent. The resultin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.4.1728-1734.2002
更新日期:2002-04-01 00:00:00
abstract::The geochemical cycling of cobalt (Co) has often been considered to be controlled by the scavenging and oxidation of Co(II) on the surface of manganese [Mn(III,IV)] oxides or manganates. Because Mn(II) oxidation in the environment is often catalyzed by bacteria, we have investigated the ability of Mn(II)-oxidizing bac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.8.2949-2957.1994
更新日期:1994-08-01 00:00:00
abstract::The discovery of a letter intentionally filled with dried Bacillus anthracis spores in the office of a United States senator prompted the collection and quarantine of all mail in congressional buildings. This mail was subsequently searched for additional intentionally contaminated letters. A microbiological sampling s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00940-06
更新日期:2006-08-01 00:00:00
abstract::Campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in Sydney, Australia. Three of four flocks examined carried C. jejuni. Eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.5.1219-1220.1982
更新日期:1982-05-01 00:00:00
abstract::Treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-Cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. Hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of wh...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.3.555-560.1982
更新日期:1982-09-01 00:00:00
abstract::Bacteria using toxic chemicals, such as detergents, as growth substrates face the challenge of exposing themselves to cell-damaging effects that require protection mechanisms, which demand energy delivered from catabolism of the toxic compound. Thus, adaptations are necessary for ensuring the rapid onset of substrate ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01352-19
更新日期:2019-11-14 00:00:00
abstract::Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.2.459-462.1983
更新日期:1983-08-01 00:00:00
abstract::A field study was conducted to determine the microbial community structures of streambed sediments across diverse geographic and climatic areas. Sediment samples were collected from three adjacent headwater forest streams within three biomes, eastern deciduous (Pennsylvania), southeastern coniferous (New Jersey), and ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01809-07
更新日期:2008-05-01 00:00:00
abstract::Although the high level of competence for natural transformation of Acinetobacter sp. strain BD413 has been the subject of numerous studies, only two competence genes, comC and comP, have been identified to date. By chromosomal walking analysis we found two overlapping open reading frames, designated comE and comF, st...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.10.4568-4574.1999
更新日期:1999-10-01 00:00:00
abstract::To understand the continuous problems that Escherichia coli O157:H7 causes as food pathogen, this study assessed global gene regulation in bacteria growing on meat. Since FlhD/FlhC of E. coli K-12 laboratory strains was previously established as a major control point in transducing signals from the environment to seve...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00069-11
更新日期:2011-06-01 00:00:00
abstract::The LlaDII restriction/modification (R/M) system was found on the naturally occurring 8.9-kb plasmid pHW393 in Lactococcus lactis subsp. cremoris W39. A 2.4-kb PstI-EcoRI fragment inserted into the Escherichia coli-L. lactis shuttle vector pCI3340 conferred to L. lactis LM2301 and L. lactis SMQ86 resistance against re...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.7.2424-2431.1998
更新日期:1998-07-01 00:00:00
abstract::Pervasive environmental stressors on coral reefs are attributed with shifting the competitive balance in favor of alternative dominants, such as macroalgae. Previous studies have demonstrated that macroalgae compete with corals via a number of mechanisms, including the production of potent primary and secondary metabo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02391-16
更新日期:2016-12-15 00:00:00
abstract::A variant of Bacillus thuringiensis subsp. kurstaki containing a single, stable copy of a uniquely amplifiable DNA oligomer integrated into the genome for tracking the fate of biological agents in the environment was developed. The use of genetically tagged spores overcomes the ambiguity of discerning the test materia...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02006-12
更新日期:2012-12-01 00:00:00
abstract:UNLABELLED:Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 log...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01669-16
更新日期:2016-09-16 00:00:00
abstract::The kinetics of adhesion of Vibrio strains isolated from diseased fish to skin mucus of gilt-head sea bream was studied. A modified Langmuir adsorption isotherm was calculated, and the results obtained indicate that the strains tested (Vibrio alginolyticus DP1HE4 and Vibrio anguillarum-like DC12R8 and DC12R9) showed a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.10.3650-3654.1996
更新日期:1996-10-01 00:00:00
abstract::Very little is known about the interaction of bryophytes with bacteria. Therefore, we analyzed bacteria associated with three bryophyte species, Tortula ruralis, Aulacomnium palustre, and Sphagnum rubellum, which represent typical moss species of three nutrient-poor plant communities at the southern Baltic Sea coast i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.11.6569-6579.2004
更新日期:2004-11-01 00:00:00
abstract::The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components. Using a cohesin biomarker, we identified a cellulosomal enzyme which belongs to the glycosyl hydrolase family 5 and has a dom...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01400-13
更新日期:2013-10-01 00:00:00
abstract::The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.8.4740-4747.2004
更新日期:2004-08-01 00:00:00
abstract::The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.7.4052-4056.2005
更新日期:2005-07-01 00:00:00
abstract::We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex geno...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02973-06
更新日期:2007-08-01 00:00:00
abstract::We show that Rhodococcus chlorophenolicus PCP-I, a polychlorophenol degrader, also degrades various chlorine-substituted guaiacols (2-methoxyphenols) and syringols (2,6-dimethoxyphenols). The substrates investigated were tetrachloroguaiacol, 3,4,6- and 3,5,6-trichloroguaiacol, 3,5- and 3,6-dichloroguaiacol, trichloros...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.3.683-687.1988
更新日期:1988-03-01 00:00:00
abstract::Low concentrations of furfural are formed as a side product during the dilute acid hydrolysis of hemicellulose. Growth is inhibited by exposure to furfural but resumes after the complete reduction of furfural to the less toxic furfuryl alcohol. Growth-based selection was used to isolate a furfural-resistant mutant of ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00567-09
更新日期:2009-07-01 00:00:00
abstract:UNLABELLED:Mechanistic studies of many processes in Agrobacterium tumefaciens have been hampered by a lack of genetic tools for characterization of essential genes. In this study, we used a Tn7-based method for inducible control of transcription from an engineered site on the chromosome. We demonstrate that this method...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01392-16
更新日期:2016-07-29 00:00:00
abstract::The introduction of transgenic plants into agricultural ecosystems has raised the question of the ecological impact of these plants on nontarget organisms, such as soil bacteria. Although differences in both the genetic structure and the metabolic function of the microbial communities associated with some transgenic p...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.12.7310-7318.2003
更新日期:2003-12-01 00:00:00
abstract::Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.12.5329-5333.2000
更新日期:2000-12-01 00:00:00
abstract::Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.5.2054-2056.1999
更新日期:1999-05-01 00:00:00
abstract::Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01900-08
更新日期:2009-03-01 00:00:00
abstract::Although biofilms represent a common bacterial lifestyle in clinically and environmentally important habitats, there is scant information on the extent of gene transfer in these spatially structured populations. The objective of this study was to gain insight into factors that affect transfer of the promiscuous multid...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00090-11
更新日期:2011-08-01 00:00:00