Determination of the efficacy of two building decontamination strategies by surface sampling with culture and quantitative PCR analysis.

Abstract:

:The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and workplace settings. An experimental room supplied with materials used in office furnishings (i.e., wood laminate, painted metal, and vinyl tile) was used with controlled dry aerosol releases of endospores of Bacillus atrophaeus ("Bacillus subtilis subsp. niger," also referred to as BG), a Bacillus anthracis surrogate. Studies were performed using two test products, a foam decontaminant and chlorine dioxide gas. Surface samples were collected pre- and posttreatment with three sampling methods and analyzed by culture and quantitative PCR (QPCR). Additional aerosol releases with environmental background present on the surface materials were also conducted to determine if there was any interference with decontamination or sample analysis. Culture results indicated that 10(5) to 10(6) CFU per sample were present on surfaces before decontamination. After decontamination with the foam, no culturable B. atrophaeus spores were detected. After decontamination with chlorine dioxide gas, no culturable B. atrophaeus was detected in 24 of 27 samples (89%). However, QPCR analysis showed that B. atrophaeus DNA was still present after decontamination with both methods. Environmental background material had no apparent effect on decontamination, but inhibition of the QPCR assay was observed. These results demonstrate the effectiveness of two decontamination methods and illustrate the utility of surface sampling and QPCR analysis for the evaluation of decontamination strategies.

journal_name

Appl Environ Microbiol

authors

Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

doi

10.1128/AEM.70.8.4740-4747.2004

keywords:

subject

Has Abstract

pub_date

2004-08-01 00:00:00

pages

4740-7

issue

8

eissn

0099-2240

issn

1098-5336

pii

70/8/4740

journal_volume

70

pub_type

杂志文章
  • Dispersion and transport of Cryptosporidium Oocysts from fecal pats under simulated rainfall events.

    abstract::The dispersion and initial transport of Cryptosporidium oocysts from fecal pats were investigated during artificial rainfall events on intact soil blocks (1,500 by 900 by 300 mm). Rainfall events of 55 mm h(-1) for 30 min and 25 mm h(-1) for 180 min were applied to soil plots with artificial fecal pats seeded with app...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.2.1151-1159.2004

    authors: Davies CM,Ferguson CM,Kaucner C,Krogh M,Altavilla N,Deere DA,Ashbolt NJ

    更新日期:2004-02-01 00:00:00

  • Effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by Enterobacter aerogenes.

    abstract::Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. Newly isolated Enterobacter aerogenes strain AJ110637, a rapid carbon source assimilator under weakly acidic (pH 5.0) conditions, was selected as a platform for succinate production. Our previo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03213-14

    authors: Tajima Y,Yamamoto Y,Fukui K,Nishio Y,Hashiguchi K,Usuda Y,Sode K

    更新日期:2015-02-01 00:00:00

  • Structure of an endo-beta-1,4-glucanase gene from Clostridium acetobutylicum P262 showing homology with endoglucanase genes from Bacillus spp.

    abstract::The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.5.1289-1292.1988

    authors: Zappe H,Jones WA,Jones DT,Woods DR

    更新日期:1988-05-01 00:00:00

  • Isolation of Vibrio cholerae serotype O1 from the eastern oyster, Crassostrea virginica.

    abstract::Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.2.559-560.1981

    authors: Hood MA,Ness GE,Rodrick GE

    更新日期:1981-02-01 00:00:00

  • Timed-release capsule method for coliform enumeration.

    abstract::Wax-coated capsules containing selective ingredients (brilliant green and oxgall) were added at the time of inoculation of most-probable-number media (modified lactose broths). The inhibitory ingredients gradually diffused from the capsules into the nonselective media, imparting selectivity to the media. Concentration...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.5.716-722.1976

    authors: Lanz WW,Hartman PA

    更新日期:1976-11-01 00:00:00

  • Characteristics of a new enantioselective thermostable dipeptidase from Brevibacillus borstelensis BCS-1 and its application to synthesis of a D-amino-acid-containing dipeptide.

    abstract::A new thermostable dipeptidase gene was cloned from the thermophile Brevibacillus borstelensis BCS-1 by genetic complementation of the D-Glu auxotroph Escherichia coli WM335 on a plate containing D-Ala-D-Glu. Nucleotide sequence analysis revealed that the gene included an open reading frame coding for a 307-amino-acid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.3.1570-1575.2004

    authors: Baek DH,Song JJ,Kwon SJ,Park C,Jung CM,Sung MH

    更新日期:2004-03-01 00:00:00

  • Proteomic analysis of extracellular proteins from Aspergillus oryzae grown under submerged and solid-state culture conditions.

    abstract::Filamentous fungi are widely used for the production of homologous and heterologous proteins. Recently, there has been increasing interest in Aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture. To provide an overview of protein secretion by A. oryzae in solid-state cultur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.5.3448-3457.2006

    authors: Oda K,Kakizono D,Yamada O,Iefuji H,Akita O,Iwashita K

    更新日期:2006-05-01 00:00:00

  • Formation of trans fatty acids is not involved in growth-linked membrane adaptation of Pseudomonas putida.

    abstract::Fatty acid compositions in growing and resting cells of several strains of Pseudomonas putida (P8, NCTC 10936, and KT 2440) were studied, with a focus on alterations of the saturation degree, cis-trans isomerization, and cyclopropane formation. The fatty acid compositions of the strains were very similar under compara...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.4.1915-1922.2005

    authors: Härtig C,Loffhagen N,Harms H

    更新日期:2005-04-01 00:00:00

  • Novel Modifications of Nonribosomal Peptides from Brevibacillus laterosporus MG64 and Investigation of Their Mode of Action.

    abstract::Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01981-20

    authors: Li Z,de Vries RH,Chakraborty P,Song C,Zhao X,Scheffers DJ,Roelfes G,Kuipers OP

    更新日期:2020-11-24 00:00:00

  • Killing of Staphylococcus aureus via Magnetic Hyperthermia Mediated by Magnetotactic Bacteria.

    abstract::Staphylococcus aureus is a common hospital and household pathogen. Given the emergence of antibiotic-resistant derivatives of this pathogen resulting from the use of antibiotics as general treatment, development of alternative therapeutic strategies is urgently needed. Here, we assess the feasibility of killing S. aur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04103-15

    authors: Chen C,Chen L,Yi Y,Chen C,Wu LF,Song T

    更新日期:2016-02-12 00:00:00

  • Investigation of genotypes of Borrelia burgdorferi in Ixodes scapularis ticks collected during surveillance in Canada.

    abstract::The genetic diversity of Borrelia burgdorferi sensu stricto, the agent of Lyme disease in North America, has consequences for the performance of serological diagnostic tests and disease severity. To investigate B. burgdorferi diversity in Canada, where Lyme disease is emerging, bacterial DNA in 309 infected adult Ixod...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02636-10

    authors: Ogden NH,Margos G,Aanensen DM,Drebot MA,Feil EJ,Hanincová K,Schwartz I,Tyler S,Lindsay LR

    更新日期:2011-05-01 00:00:00

  • Effect of transport at ambient temperature on detection and isolation of Vibrio cholerae from environmental samples.

    abstract::It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae, the causative agent of pandemic cholera. The present study was aimed at understanding the effect of transporting enviro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.3.2185-2190.2006

    authors: Alam M,Sadique A,Nur-A-Hasan,Bhuiyan NA,Nair GB,Siddique AK,Sack DA,Ahsan S,Huq A,Sack RB,Colwell RR

    更新日期:2006-03-01 00:00:00

  • Metabolic engineering of Acinetobacter baylyi ADP1 for improved growth on gluconate and glucose.

    abstract::A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01837-14

    authors: Kannisto M,Aho T,Karp M,Santala V

    更新日期:2014-11-01 00:00:00

  • Shewanella decolorationis LDS1 Chromate Resistance.

    abstract::The genus Shewanella is well known for its genetic diversity, its outstanding respiratory capacity, and its high potential for bioremediation. Here, a novel strain isolated from sediments of the Indian Ocean was characterized. A 16S rRNA analysis indicated that it belongs to the species Shewanella decolorationis It wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00777-19

    authors: Lemaire ON,Honoré FA,Tempel S,Fortier EM,Leimkühler S,Méjean V,Iobbi-Nivol C

    更新日期:2019-08-29 00:00:00

  • The plasmid-encoded lactococcal envelope-associated proteinase is encoded by a chromosomal gene in Lactococcus lactis subsp. cremoris BC101.

    abstract::The plasmid-free strain Lactococcus lactis subsp. cremoris BC101 produced an extracellular proteinase physicochemically similar to the proteinase encoded by the plasmid-linked prtP gene of other lactococcal strains. The absence of detectable plasmids in strain BC101 indicated that the prtP proteinase gene may be chrom...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.2.750-753.1992

    authors: Nissen-Meyer J,Lillehaug D,Nes IF

    更新日期:1992-02-01 00:00:00

  • Enhancing nitrite inhibition of Clostridium botulinum with isoascorbate in perishable canned cured meat.

    abstract::Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.1.59-61.1978

    authors: Tompkin RB,Christiansen LN,Shaparis AB

    更新日期:1978-01-01 00:00:00

  • Detection of Toxoplasma gondii oocysts in drinking water.

    abstract::The world's largest outbreak of waterborne toxoplasmosis occurred in a municipality in the western Canadian province of British Columbia. When drinking water emerged as a possible source of infection during the outbreak investigation, a laboratory method was needed to attempt detection of the parasite, Toxoplasma gond...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.6.2278-2280.1998

    authors: Isaac-Renton J,Bowie WR,King A,Irwin GS,Ong CS,Fung CP,Shokeir MO,Dubey JP

    更新日期:1998-06-01 00:00:00

  • Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3.

    abstract::Genome sequencing of the thermophilic archaeon Pyrococcus horikoshii OT3 revealed a gene which had high sequence similarity to the gene encoding the carboxypeptidase of Sulfolobus solfataricus and also to that encoding the aminoacylase from Bacillus stearothermophilus. The gene from P. horikoshii comprises an open rea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.2.673-679.2001

    authors: Ishikawa K,Ishida H,Matsui I,Kawarabayasi Y,Kikuchi H

    更新日期:2001-02-01 00:00:00

  • Enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production.

    abstract::When mixed ruminal bacteria were inoculated into semicontinuous cultures (25% transfer every other day) containing lactate, dulcitol, pectin, or xylose and Trypticase (1 g/liter) as the sole nitrogen source, the specific activity of ammonia production increased. The greatest enrichment was observed with lactate and xy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.4.872-877.1988

    authors: Russell JB,Strobel HJ,Chen GJ

    更新日期:1988-04-01 00:00:00

  • Trichothecene Biosynthesis in Fusarium sporotrichioides: Origin of the Oxygen Atoms of T-2 Toxin.

    abstract::Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.3.493-497.1986

    authors: Desjardins AE,Plattner RD,Vanmiddlesworth F

    更新日期:1986-03-01 00:00:00

  • Vibrio cholerae O1 strain TSI-4 produces the exopolysaccharide materials that determine colony morphology, stress resistance, and biofilm formation.

    abstract::Vibrio cholerae O1 strain TSI-4 (El Tor, Ogawa) can shift to a rugose colony morphology from its normal translucent colony morphology in response to nutrient starvation. We have investigated differences between the rugose and translucent forms of V. cholerae O1 strain TSI-4. Electron microscopic examination of the rug...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.3648-3655.1998

    authors: Wai SN,Mizunoe Y,Takade A,Kawabata SI,Yoshida SI

    更新日期:1998-10-01 00:00:00

  • Novel method for purification of staphylococcal enterotoxin A.

    abstract::A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.7.1761-1765.1988

    authors: Reynolds D,Tranter HS,Sage R,Hambleton P

    更新日期:1988-07-01 00:00:00

  • Tracing of norovirus outbreak strains in mussels collected near sewage effluents.

    abstract::Noroviruses from mussels collected near sewage effluents were compared with local patient outbreak strains. Sequence analyses of RNA polymerase-capsid-poly(A)-3' (3.1-kilobase) regions confirmed the 99.9% similarity between genotype I.1 strains from mussels and patient strains from recreational-bathing outbreaks, indi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02477-07

    authors: Nenonen NP,Hannoun C,Horal P,Hernroth B,Bergström T

    更新日期:2008-04-01 00:00:00

  • Studies on the production of fungal peroxidases in Aspergillus niger.

    abstract::To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-defi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.7.3016-3023.2000

    authors: Conesa A,van den Hondel CA,Punt PJ

    更新日期:2000-07-01 00:00:00

  • Exopolysaccharide-Deficient Mutants of Rhizobium fredii HH303 Which Are Symbiotically Effective.

    abstract::Nineteen Tn5-induced mutants of Rhizobium fredii HH303 defective in acidic exopolysaccharide synthesis were isolated by screening for lack of Calcofluor fluorescence. They were grouped by complementation analysis by using Rhizobium meliloti cosmids carrying exo genes. All of the 19 mutants were symbiotically effective...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.7.1852-1854.1989

    authors: Kim CH,Tully RE,Keister DL

    更新日期:1989-07-01 00:00:00

  • Polyphenolic extract from maple syrup potentiates antibiotic susceptibility and reduces biofilm formation of pathogenic bacteria.

    abstract::Phenolic compounds are believed to be promising candidates as complementary therapeutics. Maple syrup, prepared by concentrating the sap from the North American maple tree, is a rich source of natural and process-derived phenolic compounds. In this work, we report the antimicrobial activity of a phenolic-rich maple sy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00239-15

    authors: Maisuria VB,Hosseinidoust Z,Tufenkji N

    更新日期:2015-06-01 00:00:00

  • Novel psychropiezophilic Oceanospirillales species Profundimonas piezophila gen. nov., sp. nov., isolated from the deep-sea environment of the Puerto Rico trench.

    abstract::The diversity of deep-sea high-pressure-adapted (piezophilic) microbes in isolated monoculture remains low. In this study, a novel obligately psychropiezophilic bacterium was isolated from seawater collected from the Puerto Rico Trench at a depth of ∼6,000 m. This isolate, designated YC-1, grew best in a nutrient-rich...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02288-13

    authors: Cao Y,Chastain RA,Eloe EA,Nogi Y,Kato C,Bartlett DH

    更新日期:2014-01-01 00:00:00

  • Kinetic Analyses of Desulfurization of Dibenzothiophene by Rhodococcus erythropolis in Continuous Cultures.

    abstract::Rhodococcus erythropolis N1-36, a desulfurization strain, was grown in continuous culture at 10 different dilution rates with 50 (mu)M dibenzothiophene sulfone (DBTO(inf2)) as the growth-limiting nutrient. The steady-state biomass, concentrations of substrate (DBTO(inf2)) and product (monohydroxybiphenyl), saturation ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.8.3066-3068.1996

    authors: Wang P,Humphrey AE,Krawiec S

    更新日期:1996-08-01 00:00:00

  • Nitric Oxide Improves the Tolerance of Pleurotus ostreatus to Heat Stress by Inhibiting Mitochondrial Aconitase.

    abstract::Pleurotus ostreatus is widely cultivated in China. However, its cultivation is strongly affected by seasonal temperature changes, especially the high temperatures of summer. Nitric oxide (NO) was previously reported to alleviate oxidative damage to mycelia by regulating trehalose. In this study, we found that NO allev...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02303-19

    authors: Hou L,Zhao M,Huang C,Wu X,Zhang J

    更新日期:2020-02-18 00:00:00

  • Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms.

    abstract::Carbon dioxide fixation is carried out primarily through the Calvin-Benson-Bassham reductive pentose phosphate cycle, in which ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) is the key enzyme. The primary structure of the large subunit of form I RubisCO is well conserved; however, four distinct types, A, B...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.6.1913-1921.1996

    authors: Xu HH,Tabita FR

    更新日期:1996-06-01 00:00:00