Kinetic Analyses of Desulfurization of Dibenzothiophene by Rhodococcus erythropolis in Continuous Cultures.

abstract：:Escherichia coli strains harboring deletion mutations of the insecticidal protoxin gene of Bacillus thuringiensis subsp. berliner 1715 were constructed. Although these strains did not produce intact protoxin, cell extracts from one of the mutants were extremely toxic to tobacco hornworm (Manduca sexta) larvae, indicat...

journal_title：Applied and environmental microbiology

authors： Wabiko H,Held GA,Bulla LA Jr

更新日期：1985-03-01 00:00:00

abstract：:The transfer of naturally occurring conjugative plasmids from the indigenous microflora to a genetically modified population of bacteria colonizing the phytospheres of plants has been observed. The marked strain (Pseudomonas fluorescens SBW25EeZY6KX) was introduced as a seed dressing to sugar beets (Beta vulgaris var....

journal_title：Applied and environmental microbiology

authors： Lilley AK,Bailey MJ

更新日期：1997-04-01 00:00:00

abstract：:Minute amounts of oxygen were supplied to a continuous cultivation of Lactococcus lactis subsp. cremoris MG1363 grown on a defined glucose-limited medium at a dilution rate of 0.1 h(-1). More than 80% of the carbon supplied with glucose ended up in fermentation products other than lactate. Addition of even minute amou...

journal_title：Applied and environmental microbiology

authors： Jensen NB,Melchiorsen CR,Jokumsen KV,Villadsen J

更新日期：2001-06-01 00:00:00

abstract：:Low-molecular-weight (LMW) RNA molecules were analyzed to characterize rhizobial isolates that nodulate the common bean growing in Spain. Since LMW RNA profiles, determined by staircase electrophoresis, varied across the rhizobial species nodulating beans, we demonstrated that bean isolates recovered from Spanish soil...

journal_title：Applied and environmental microbiology

authors： Velázquez E,Martínez-Romero E,Rodríguez-Navarro DN,Trujillo ME,Daza A,Mateos PF,Martínez-Molina E,van Berkum P

更新日期：2001-02-01 00:00:00

abstract：:Pseudomonas fluorescens isolates 13525 and 2-79 were grown in Luria broth and low-nutrient medium (LNM). Pililike fibrils were very rarely produced in Luria broth but were abundantly produced in LNM. In LNM the pili were peritrichously distributed and had diameters ranging from 3 to 8 nm. Pili were purified from strai...

journal_title：Applied and environmental microbiology

authors： Vesper SJ

更新日期：1987-07-01 00:00:00

abstract：:The insertion of a heterologous gene into commensal bacteria is a common technique to develop a delivery agent for vaccination and therapies, but the pleiotropic effects of genetic modifications need to be investigated before its use in practical applications. Although supplemental properties provided by the expressio...

journal_title：Applied and environmental microbiology

authors： Kajikawa A,Igimi S

更新日期：2009-05-01 00:00:00

abstract：:The effects of limiting concentrations of ammonium on the metabolic activity of Nitrosomonas europaea, an obligate ammonia-oxidizing soil bacterium, were investigated. Cells were harvested during late logarithmic growth and were incubated for 24 h in growth medium containing 0, 15, or 50 mM ammonium. The changes in ni...

journal_title：Applied and environmental microbiology

authors： Stein LY,Arp DJ

更新日期：1998-04-01 00:00:00

abstract：:We evaluated a ready-to-use real-time quantitative Legionella pneumophila PCR assay system by testing 136 hot-water-system samples collected from 55 sites as well as 49 cooling tower samples collected from 20 different sites, in parallel with the standard culture method. The PCR assay was reproducible and suitable for...

journal_title：Applied and environmental microbiology

authors： Yaradou DF,Hallier-Soulier S,Moreau S,Poty F,Hillion Y,Reyrolle M,André J,Festoc G,Delabre K,Vandenesch F,Etienne J,Jarraud S

更新日期：2007-03-01 00:00:00

abstract：:Xenorhabdus nematophila engages in a mutualistic association with the nematode Steinernema carpocapsae. The nematode invades and traverses the gut of susceptible insects. X. nematophila is released in the insect blood (hemolymph), where it suppresses host immune responses and functions as a pathogen. X. nematophila pr...

journal_title：Applied and environmental microbiology

authors： Singh S,Reese JM,Casanova-Torres AM,Goodrich-Blair H,Forst S

更新日期：2014-07-01 00:00:00

abstract：:Different Rhizobium and Bradyrhizobium strains were screened for their ability to produce melanin. Pigment producers (Mel) were found among strains of R. leguminosarum biovars viceae, trifolii, and phaseoli, R. meliloti, and R. fredii; none of 19 Bradyrhizobium strains examined gave a positive response. Melanin produc...

journal_title：Applied and environmental microbiology

authors： Cubo MT,Buendia-Claveria AM,Beringer JE,Ruiz-Sainz JE

更新日期：1988-07-01 00:00:00

abstract：:Streptococcus agalactiae (group B Streptococcus [GBS]) is a cause of severe infections, particularly during the newborn period. While methods exist for generating chromosomal mutations in GBS, they are cumbersome and inefficient and present significant challenges if the goal is to study subtle mutations, such as singl...

journal_title：Applied and environmental microbiology

authors： Hooven TA,Bonakdar M,Chamby AB,Ratner AJ

更新日期：2019-03-22 00:00:00

abstract：:Laboratory-scale soil microcosms containing different soils were permeated with CH(inf4) for up to 6 months to investigate their capacity to develop a methanotrophic community. Methane emissions were monitored continuously until steady states were established. The porous, coarse sand soil developed the greatest methan...

journal_title：Applied and environmental microbiology

authors： Kightley D,Nedwell DB,Cooper M

更新日期：1995-02-01 00:00:00

abstract：:A toluene-degrading sulfate-reducing bacterium, strain Tol2, was isolated from marine sediment under strictly anoxic conditions. Toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mM. To provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic...

journal_title：Applied and environmental microbiology

authors： Rabus R,Nordhaus R,Ludwig W,Widdel F

更新日期：1993-05-01 00:00:00

abstract：:The ABC transporter (TliDEF) from Pseudomonas fluorescens SIK W1, which mediated the secretion of a thermostable lipase (TliA) into the extracellular space in Escherichia coli, was engineered using directed evolution (error-prone PCR) to improve its secretion efficiency. TliD mutants with increased secretion efficienc...

journal_title：Applied and environmental microbiology

authors： Eom GT,Song JK,Ahn JH,Seo YS,Rhee JS

更新日期：2005-07-01 00:00:00

abstract：:Medium-chain-length (mcl) poly(3-hydroxyalkanoates) (PHAs) are storage polymers that are produced from various substrates and accumulate in Pseudomonas strains belonging to rRNA homology group I. In experiments aimed at increasing PHA production in Pseudomonas strains, we generated an mcl PHA-overproducing mutant of P...

journal_title：Applied and environmental microbiology

authors： Klinke S,Dauner M,Scott G,Kessler B,Witholt B

更新日期：2000-03-01 00:00:00

abstract：:Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

journal_title：Applied and environmental microbiology

authors： Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

更新日期：2007-10-01 00:00:00

abstract：:We examined the potential use of natural-abundance stable carbon isotope ratios of lipids for determining substrate usage by sulfate-reducing bacteria (SRB). Four SRB were grown under autotrophic, mixotrophic, or heterotrophic growth conditions, and the delta13C values of their individual fatty acids (FA) were determi...

journal_title：Applied and environmental microbiology

authors： Londry KL,Jahnke LL,Des Marais DJ

更新日期：2004-02-01 00:00:00

abstract：:Fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. We examined 72 Fusarium isolates from a native North American tallgrass prairie and found that Gibberella inter...

journal_title：Applied and environmental microbiology

authors： Leslie JF,Zeller KA,Logrieco A,Mulè G,Moretti A,Ritieni A

更新日期：2004-04-01 00:00:00

abstract：:Eighteen strains of Agrobacterium tumefaciens isolated from crown galls were tested for agrocin production. Of six agrocin-producing strains, one (D286) produced a broad-host-range agrocin active against strains carrying nopaline, octopine, and agropine type Ti plasmids. Sensitivity to agrocin D286 was found to map in...

journal_title：Applied and environmental microbiology

authors： Hendson M,Askjaer L,Thomson JA,van Montagu M

更新日期：1983-05-01 00:00:00

abstract：:Microcosms were prepared from aquifer material, spiked with monoaromatic hydrocarbons, and amended with oxygen, nitrate, and nitrous oxide. Benzene and alkylbenzenes were degraded to concentrations below 5 micrograms/liter within 7 days under aerobic conditions, whereas only the alkylbenzenes were degraded when either...

journal_title：Applied and environmental microbiology

authors： Hutchins SR

更新日期：1991-08-01 00:00:00

abstract：:Seventy-two Listeria monocytogenes isolates originating from 10 different fish products of 12 producers and 47 isolates from human listeriosis cases were typed by serotyping and multilocus enzyme electrophoresis. Seventy-five of these isolates were further subtyped by restriction analysis of genomic DNA with the enzym...

journal_title：Applied and environmental microbiology

authors： Boerlin P,Boerlin-Petzold F,Bannerman E,Bille J,Jemmi T

更新日期：1997-04-01 00:00:00

abstract：:When carbon sources are changed, Saccharomyces cerevisiae transcriptional patterns drastically change. To identify genes whose transcription can be used to quantitatively measure sugar concentrations, we searched genomic expression databases for a set of genes that are highly induced during the diauxic shift, and we u...

journal_title：Applied and environmental microbiology

authors： Chambers P,Issaka A,Palecek SP

更新日期：2004-01-01 00:00:00

abstract：:Three bacteria capable of utilizing bis(4-hydroxyphenyl)methane (bisphenol F [BPF]) as the sole carbon source were isolated from river water, and they all belonged to the family Sphingomonadaceae. One of the isolates, designated Sphingobium yanoikuyae strain FM-2, at an initial cell density of 0.01 (optical density at...

journal_title：Applied and environmental microbiology

authors： Inoue D,Hara S,Kashihara M,Murai Y,Danzl E,Sei K,Tsunoi S,Fujita M,Ike M

更新日期：2008-01-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:Bacterioplankton numbers, biomasses, and productivities, as well as chlorophyll a concentrations and phytoplankton productivities, were assayed from 1 March 1984 to 12 August 1985 through a 250-m-deep seawater column in Howe Sound, a temperate fjord-sound on the southern coast of British Columbia, Canada. Primary prod...

journal_title：Applied and environmental microbiology

authors： Albright LJ,McCrae SK

更新日期：1987-06-01 00:00:00

abstract：:Preinfection development in Colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus Colletotrichum gloeosporioides f. sp. aeschynomene was shown to ...

journal_title：Applied and environmental microbiology

authors： Buhr TL,Dickman MB

更新日期：1997-06-01 00:00:00

abstract：:The use of Lactococcus lactis (the most extensively characterized lactic acid bacterium) as a delivery organism for heterologous proteins is, in some cases, limited by low production levels and poor-quality products due to surface proteolysis. In this study, we combined in one L. lactis strain use of the nisin-inducib...

journal_title：Applied and environmental microbiology

authors： Miyoshi A,Poquet I,Azevedo V,Commissaire J,Bermudez-Humaran L,Domakova E,Le Loir Y,Oliveira SC,Gruss A,Langella P

更新日期：2002-06-01 00:00:00

abstract：:A rapid coliphage analysis technique for enumerating coliphages in natural waters has been evaluated by water quality laboratories located throughout the United States. Correlations were established between coliphages and coliforms in natural water systems. These correlations were highly significant. This relationship...

journal_title：Applied and environmental microbiology

authors： Wentsel RS,O'Neill PE,Kitchens JF

更新日期：1982-02-01 00:00:00

abstract：:Virus adsorption to soils is considered to be the most important factor in removing viruses after land treatment of wastewater. Most of the studies on virus adsorption to soils have utilized poliovirus as the model system. In the present study, comparative adsorption of a number of different types and strains of human...

journal_title：Applied and environmental microbiology

authors： Goyal SM,Gerba CP

更新日期：1979-08-01 00:00:00

abstract：:The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW expo...

journal_title：Applied and environmental microbiology

authors： Rougier C,Prorot A,Chazal P,Leveque P,Leprat P

更新日期：2014-08-01 00:00:00