Reduction of tumor necrosis factor alpha-inducing capacity of recombinant Lactobacillus casei via expression of Salmonella OmpC.

abstract：:Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

journal_title：Applied and environmental microbiology

authors： Bernhard AE,Field KG

更新日期：2000-10-01 00:00:00

abstract：:Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other ty...

journal_title：Applied and environmental microbiology

authors： Rezzonico F,Défago G,Moënne-Loccoz Y

更新日期：2004-09-01 00:00:00

abstract：:Bacterial isolates from frogs were phenotypically identified as Ochrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity with Brucella inopinata. Further analysis of recA, omp2a, omp2b, bcsp31, and IS711 and multilocus sequence analysis (MLSA) verified a close relationship with Brucella, suggesting th...

journal_title：Applied and environmental microbiology

authors： Eisenberg T,Hamann HP,Kaim U,Schlez K,Seeger H,Schauerte N,Melzer F,Tomaso H,Scholz HC,Koylass MS,Whatmore AM,Zschöck M

更新日期：2012-05-01 00:00:00

abstract：:Six microorganisms that produced acetoin or diacetyl or both from glucose were tested for the production of 2,3-butylene glycol from lactose. Bacillus polymyxa and Streptococcus faecalis gave positive results and were tested in unmodified wheys. Cottage cheese whey was unsatisfactory, but B. polymyxa produced large am...

journal_title：Applied and environmental microbiology

authors： Speckman RA,Collins EB

更新日期：1982-05-01 00:00:00

abstract：:Three particulate methane monooxygenase PCR primer sets (A189-A682, A189-A650, and A189-mb661) were investigated for their ability to assess methanotroph diversity in soils from three sites, i.e., heath, oak, and sitka, each of which was capable of oxidizing atmospheric concentrations of methane. Each PCR primer set w...

journal_title：Applied and environmental microbiology

authors： Bourne DG,McDonald IR,Murrell JC

更新日期：2001-09-01 00:00:00

abstract：:Among the Frankia strains capable of establishing a nitrogen-fixing symbiosis with actinorrhizal plants, in planta sporangial formation is not universal and has led to the distinction between spore-positive (Sp+) and spore-negative (Sp-) nodules. Numerous Frankia strains have been isolated in pure culture from Sp+ nod...

journal_title：Applied and environmental microbiology

authors： Simonet P,Bosco M,Chapelon C,Moiroud A,Normand P

更新日期：1994-04-01 00:00:00

abstract：:This investigation introduces the use of an immunoglobulin A mouse myeloma protein for the detection of Salmonella spp. in milk. The immunoglobulin A protein M467 reacts with flagellin from a wide variety of serotypes. Two assays were developed which used an enzyme-linked immunosorbent assay (ELISA) and M467. Alkaline...

journal_title：Applied and environmental microbiology

authors： Smith AM,Jones C

更新日期：1983-10-01 00:00:00

abstract：:A methanotroph (strain 68-1), originally isolated from a trichloroethylene (TCE)-contaminated aquifer, was identified as the type I methanotroph Methylomonas methanica on the basis of intracytoplasmic membrane ultrastructure, phospholipid fatty acid profile, and 16S rRNA signature probe hybridization. Strain 68-1 was ...

journal_title：Applied and environmental microbiology

authors： Koh SC,Bowman JP,Sayler GS

更新日期：1993-04-01 00:00:00

abstract：:Indirect pathogenicity (IP), the commensal protection of antibiotic-sensitive pathogens by resistant microorganisms of low intrinsic virulence, can prevent the eradication of polymicrobial infections. The contributions of antibiotic resistance mechanisms and biofilm structure to IP within polymicrobial biofilms were i...

journal_title：Applied and environmental microbiology

authors： O'Connell HA,Kottkamp GS,Eppelbaum JL,Stubblefield BA,Gilbert SE,Gilbert ES

更新日期：2006-07-01 00:00:00

abstract：:A membrane filter technique has been developed for the enumeration of bifidobacteria in natural aquatic environments. The technique is quantitative, selective, and differential. The medium (YN-6) contains: yeast extract, 2.0 g; agar, 1.5 g; polypeptone peptone, 1.0 g; vitamin-free Casamino Acids, 0.8 g; sodium chlorid...

journal_title：Applied and environmental microbiology

authors： Resnick IG,Levin MA

更新日期：1981-09-01 00:00:00

abstract：:Pseudomonas fluorescens 5R contains an NAH7-like plasmid (pKA1), and P. fluorescens 5R mutant 5RL contains a bioluminescent reporter plasmid (pUTK21) which was constructed by transposon mutagenesis. Polymerase chain reaction mapping confirmed the localization of lux transposon Tn4431 300 bp downstream from the start o...

journal_title：Applied and environmental microbiology

authors： Menn FM,Applegate BM,Sayler GS

更新日期：1993-06-01 00:00:00

abstract：:Sodium salicylate and ammonium sulfate were applied to leaf surfaces along with suspensions of the biological control agents Pseudomonas syringae Cit7(pNAH7), which catabolizes salicylate, and Cit7, which does not catabolize salicylate, to determine whether enhanced biological control of bacterial speck of tomato coul...

journal_title：Applied and environmental microbiology

authors： Ji P,Wilson M

更新日期：2003-02-01 00:00:00

abstract：:Several variations in the scintillation mixture and the filter paper arrangements for double-vial radiorespirometry were compared. Improved efficiencies (44%) and shorter response times were found by adding wetting agents and methanolic NaOH to the scintillation mixture in the filter paper. The scintillation chemicals...

journal_title：Applied and environmental microbiology

authors： McKinley VL,Federle TW,Vestal JR

更新日期：1983-01-01 00:00:00

abstract：:Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). This represents a new approach to increase furfural tolerance and may be usef...

journal_title：Applied and environmental microbiology

authors： Geddes RD,Wang X,Yomano LP,Miller EN,Zheng H,Shanmugam KT,Ingram LO

更新日期：2014-10-01 00:00:00

abstract：:Prior growth of seven enterohemorrhagic and one nonenterohemorrhagic strains of Escherichia coli in tryptic soy broth with (TSB+G) and without (TSB-G) 1% glucose was evaluated for its effect on acid tolerance. The final pHs of 18-h TSB+G and TSB-G cultures were 4.6 to 5.2 and 6.9 to 7.0, respectively. Cells were then ...

journal_title：Applied and environmental microbiology

authors： Buchanan RL,Edelson SG

更新日期：1996-11-01 00:00:00

abstract：:The effects of aflatoxin on egg production, fertility, hatchability, and progeny performance were investigated by feeding dietary aflatoxin at dose levels of 0,5, and 10 mug/g to mature broiler breeder hens for 4 weeks. Sixteen hens were used for each dietary dose level. Egg production decreased significantly during w...

journal_title：Applied and environmental microbiology

authors： Howarth B Jr,Wyatt RD

更新日期：1976-05-01 00:00:00

abstract：:We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and even...

journal_title：Applied and environmental microbiology

authors： Sakai S,Imachi H,Sekiguchi Y,Tseng IC,Ohashi A,Harada H,Kamagata Y

更新日期：2009-07-01 00:00:00

abstract：:Peramine is an insect-feeding deterrent produced by Epichloë species in symbiotic association with C3 grasses. The perA gene responsible for peramine synthesis encodes a two-module nonribosomal peptide synthetase. Alleles of perA are found in most Epichloë species; however, peramine is not produced by many perA-contai...

journal_title：Applied and environmental microbiology

authors： Berry D,Takach JE,Schardl CL,Charlton ND,Scott B,Young CA

更新日期：2015-04-01 00:00:00

abstract：:A number of chemical, microbial, and eukaryotic indicators have been proposed as indicators of fecal pollution sources in water bodies. No single one of the indicators tested to date has been able to determine the source of fecal pollution in water. However, the combined use of different indicators has been demonstrat...

journal_title：Applied and environmental microbiology

authors： Ballesté E,Bonjoch X,Belanche LA,Blanch AR

更新日期：2010-03-01 00:00:00

abstract：:Leghorn hens over 50 weeks of age were assigned to two treatment groups designated as either unmolted controls or molted. A forced molt was induced by a 9-day feed withdrawal, and each hen was challenged orally with 10(5) Salmonella enteritidis organisms on day 4 of feed withdrawal. On days 4 and 9 of molt, the number...

journal_title：Applied and environmental microbiology

authors： Durant JA,Corrier DE,Byrd JA,Stanker LH,Ricke SC

更新日期：1999-05-01 00:00:00

abstract：:Tn5 insertion mutants of a soil isolate, Pseudomonas fluorescens Pf0-1, were selected for decreased ability to adhere to quartz sand in a column assay. Three adhesion-deficient mutants that differed in the location of the Tn5 insertion in the chromosome were isolated and compared with the wild-type strain. One mutant,...

journal_title：Applied and environmental microbiology

authors： Deflaun MF,Marshall BM,Kulle EP,Levy SB

更新日期：1994-07-01 00:00:00

abstract：:Homologous recombination plays an important role in the structuring of genetic variation of many bacteria; however, its importance in adaptive evolution is not well established. We investigated the association of intersubspecific homologous recombination (IHR) with the shift to a novel host (mulberry) by the plant-pat...

journal_title：Applied and environmental microbiology

authors： Nunney L,Schuenzel EL,Scally M,Bromley RE,Stouthamer R

更新日期：2014-05-01 00:00:00

abstract：:A novel Bacillus thuringiensis crystal protein with a silent activity against the Colorado potato beetle is described. The crystal proteins are produced as bipyramidal crystals. These crystals contain a protein of 129 kDa with a trypsin-resistant core fragment of 72 kDa. Neither a spore-crystal mixture nor in vitro-so...

journal_title：Applied and environmental microbiology

authors： Lambert B,Höfte H,Annys K,Jansens S,Soetaert P,Peferoen M

更新日期：1992-08-01 00:00:00

abstract：:Listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 L. monocytogenes mutants was screened, using a micro...

journal_title：Applied and environmental microbiology

authors： Hingston PA,Piercey MJ,Truelstrup Hansen L

更新日期：2015-08-15 00:00:00

abstract：:Sputter coating of leaf litter microbe samples provides scanning electron microscope images with greater information content than either vacuum evaporation of thin metal coatings or tissue conductance. ...

journal_title：Applied and environmental microbiology

authors： Draggan S

更新日期：1976-02-01 00:00:00

abstract：:Plasmodium falciparum, in addition to scavenging essential fatty acids from its intra- and intercellular environments, possesses a functional complement of type II fatty acid synthase (FAS) enzymes targeted to the apicoplast organelle. Recent evidence suggests that products of the plasmodial FAS II system may be criti...

journal_title：Applied and environmental microbiology

authors： Du Y,Gisselberg JE,Johnson JD,Lee PJ,Prigge ST,Bachmann BO

更新日期：2010-06-01 00:00:00

abstract：:The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

journal_title：Applied and environmental microbiology

authors： Li J,Hu X,Yan J,Yuan Z

更新日期：2009-06-01 00:00:00

abstract：:Statistical models were used to predict the effects of tryptone, glucose, yeast extract (TGY) and Mn on biomass formation of the highly radioresistant bacterium Deinococcus radiodurans. Results suggested that glucose had marginal effect on biomass buildup, but Mn was a significant factor for biomass formation. Mn also...

journal_title：Applied and environmental microbiology

authors： Zhang YM,Wong TY,Chen LY,Lin CS,Liu JK

更新日期：2000-01-01 00:00:00

abstract：:The effect of the glutathione reductase (GshR) activity of Lactobacillus sanfranciscensis DSM20451(T) on the thiol levels in fermented sourdoughs was determined, and the oxygen tolerance of the strain was also determined. The gshR gene coding for a putative GshR was sequenced and inactivated by single-crossover integr...

journal_title：Applied and environmental microbiology

authors： Jänsch A,Korakli M,Vogel RF,Gänzle MG

更新日期：2007-07-01 00:00:00

abstract：:Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

journal_title：Applied and environmental microbiology

authors： Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

更新日期：1987-05-01 00:00:00