Abstract:
:A toluene-degrading sulfate-reducing bacterium, strain Tol2, was isolated from marine sediment under strictly anoxic conditions. Toluene was toxic if applied directly to the medium at concentrations higher than 0.5 mM. To provide toluene continuously at a nontoxic concentration, it was supplied in an inert hydrophobic carrier phase. The isolate had oval, sometimes motile cells (1.2 to 1.4 by 1.2 to 2.0 microns). The doubling time was 27 h. Toluene was completely oxidized to CO2, as demonstrated by measurement of the degradation balance. The presence of carbon monoxide dehydrogenase and formate dehydrogenase indicated a terminal oxidation of acetyl coenzyme A via the CO dehydrogenase pathway. The use of hypothetical intermediates of toluene degradation was tested in growth experiments and adaptation studies with dense cell suspensions. Results do not support a degradation of toluene via one of the cresols or methylbenzoates, benzyl alcohol, or phenylacetate as free intermediate. Benzyl alcohol did not serve as growth substrate; moreover, it was a strong, specific inhibitor of toluene degradation, whereas benzoate utilization was not affected by benzyl alcohol. Sequencing of 16S rRNA revealed a relationship to the metabolically dissimilar genus Desulfobacter and on a deeper level to the genus Desulfobacterium. The new genus and species Desulfobacula toluolica is proposed.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Rabus R,Nordhaus R,Ludwig W,Widdel Fdoi
10.1128/AEM.59.5.1444-1451.1993subject
Has Abstractpub_date
1993-05-01 00:00:00pages
1444-51issue
5eissn
0099-2240issn
1098-5336journal_volume
59pub_type
杂志文章abstract::Gene clusters for biosynthesis of the fungal polyketides hypothemycin and radicicol from Hypomyces subiculosus and Pochonia chlamydosporia, respectively, were sequenced. Both clusters encode a reducing polyketide synthase (PKS) and a nonreducing PKS like those in the zearalenone cluster of Gibberella zeae, plus enzyme...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00478-08
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03811-13
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pub_type: 杂志文章
doi:10.1128/aem.67.10.4901-4907.2001
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.60.3.1029-1032.1994
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.1.312-314.1981
更新日期:1981-01-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1981-10-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1979-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.1.246-251.1989
更新日期:1989-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02145-15
更新日期:2015-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.3.743-747.1983
更新日期:1983-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.10.3378-3384.1993
更新日期:1993-10-01 00:00:00
abstract::A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...
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pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.32.2.206-208.1976
更新日期:1976-08-01 00:00:00
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更新日期:2016-08-15 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.6.1548-1549.1985
更新日期:1985-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.2.750-753.1992
更新日期:1992-02-01 00:00:00
abstract::Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.40.6.1044-1048.1980
更新日期:1980-12-01 00:00:00
abstract::The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.1.65-72.2002
更新日期:2002-01-01 00:00:00
abstract::This study quantified the levels of airborne microorganisms in six swine farms with more than 10,000 pigs in subtropical Taiwan. We evaluated breeding, growing, and finishing stalls, which were primarily open-air buildings, as well as partially enclosed farrowing and nursery piggeries. Airborne culturable bacteria, gr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
abstract::Freshwater, sewage, and fecal samples from various sources were examined for Rhodococcus coprophilus, associated actinomycetes, Escherichia coli, and fecal streptococci. Rhodococcus coprophilus was isolated consistently from feces of farm animals, poultry reared in proximity to farm animals, freshwater, and wastewater...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.6.1037-1042.1981
更新日期:1981-12-01 00:00:00
abstract::Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01632-07
更新日期:2008-03-01 00:00:00
abstract::Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.1.255-257.1982
更新日期:1982-07-01 00:00:00