Characterization of Pseudomonas spp. associated with spoilage of gilt-head sea bream stored under various conditions.

Abstract:

:The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20 degrees C) and packaging conditions (air and a modified atmosphere of 40% CO(2)-30% N(2)-30% O(2)). Pseudomonas lundensis was the predominant species, followed by Pseudomonas fluorescens, while Pseudomonas fragi and Pseudomonas putida were detected less frequently. Fluorescent Pseudomonas strains dominated under air conditions, while proteolytic and less lipolytic strains dominated under modified-atmosphere packaging. Different storage conditions appear to govern the selection of pseudomonads in gilt-head sea bream fish.

journal_name

Appl Environ Microbiol

authors

Tryfinopoulou P,Tsakalidou E,Nychas GJ

doi

10.1128/aem.68.1.65-72.2002

keywords:

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

65-72

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

杂志文章
  • Influence of incubation temperature on the microbial reductive dechlorination of 2,3,4,6-tetrachlorobiphenyl in two freshwater sediments.

    abstract::Volumn 62, no. 11, p. 4174, Abstract, lines 12 and 13: "para dechlorination was restricted from 18 to 34(deg)C" should read "para dechlorination was restricted to temperatures from 18 to 34(deg)C." [This corrects the article on p. 4174 in vol. 62.]. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:

    authors: Wu Q,Bedard DL,Wiegel J

    更新日期:1997-02-01 00:00:00

  • In situ analysis of denitrifying toluene- and m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column.

    abstract::A diesel fuel-contaminated aquifer was bioremediated in situ by the injection of oxidants (O2 and NO3-) and nutrients in order to stimulate microbial activity. After 3.5 years of remediation, an aquifer sample was excavated and the material was used (i) to isolate bacterial strains able to grow on selected hydrocarbon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.6.2136-2141.1997

    authors: Hess A,Zarda B,Hahn D,Häner A,Stax D,Höhener P,Zeyer J

    更新日期:1997-06-01 00:00:00

  • The presence of icaADBC is detrimental to the colonization of human skin by Staphylococcus epidermidis.

    abstract::Previous studies have demonstrated that Staphylococcus epidermidis isolates colonizing the skin of healthy humans do not typically encode icaADBC, the genes responsible for the production of polysaccharide intercellular adhesin or biofilms. It was therefore hypothesized that the presence of icaADBC was deleterious to ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01017-08

    authors: Rogers KL,Rupp ME,Fey PD

    更新日期:2008-10-01 00:00:00

  • Autoradiographic Studies of [methyl-H]Thymidine Incorporation in a Cyanobacterium (Microcystis wesenbergii)-Bacterium Association and in Selected Algae and Bacteria.

    abstract::The present investigation showed by means of autoradiography that the cyanobacterium Microcystis wesenbergii did not incorporate [H]thymidine at nanomolar concentrations, whereas its associated heterotrophic bacteria appearing in the gelatinous cover of the cyanobacterium became labeled. Several other tested cyaobacte...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.1.232-233.1985

    authors: Bern L

    更新日期:1985-01-01 00:00:00

  • Global transcriptional response of Nitrosomonas europaea to chloroform and chloromethane.

    abstract::Upon exposure of Nitrosomonas europaea to chloroform (7 microM, 1 h), transcripts for 175 of 2,460 genes were found at higher levels in treated cells than in untreated cells and transcripts for 501 genes were found at lower levels. With chloromethane (3.2 mM, 1 h), transcripts for 67 genes were at higher levels and tr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02831-06

    authors: Gvakharia BO,Permina EA,Gelfand MS,Bottomley PJ,Sayavedra-Soto LA,Arp DJ

    更新日期:2007-05-01 00:00:00

  • A phosphonate-induced gene which promotes Penicillium-mediated bioconversion of cis-propenylphosphonic acid to fosfomycin.

    abstract::Penicillium decumbens is able to epoxidize cis-propenylphosphonic acid (cPA) to produce the antibiotic fosfomycin [FOM; also referred to as phosphonomycin and (-)-cis-1,2-epoxypropylphosphonic acid], a bioconversion of considerable commercial significance. We sought to improve the efficiency of the process by overexpr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.3.1036-1044.1999

    authors: Watanabe M,Sumida N,Murakami S,Anzai H,Thompson CJ,Tateno Y,Murakami T

    更新日期:1999-03-01 00:00:00

  • Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil.

    abstract::The root-colonizing bacterium Pseudomonas fluorescens CHA0 was used to construct an oxygen-responsive biosensor. An anaerobically inducible promoter of Pseudomonas aeruginosa, which depends on the FNR (fumarate and nitrate reductase regulation)-like transcriptional regulator ANR (anaerobic regulation of arginine deimi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4085-4093.1999

    authors: Højberg O,Schnider U,Winteler HV,Sørensen J,Haas D

    更新日期:1999-09-01 00:00:00

  • Detection and characterization of cyanobacterial nifH genes.

    abstract::The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.3.880-887.1994

    authors: Ben-Porath J,Zehr JP

    更新日期:1994-03-01 00:00:00

  • Indirect Immunodetection of Fungal Fragments by Field Emission Scanning Electron Microscopy.

    abstract::Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse healt...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00929-15

    authors: Afanou KA,Straumfors A,Skogstad A,Nayak AP,Skaar I,Hjeljord L,Tronsmo A,Eduard W,Green BJ

    更新日期:2015-09-01 00:00:00

  • Biodiversity of exopolysaccharides produced by Streptococcus thermophilus strains is reflected in their production and their molecular and functional characteristics.

    abstract::Twenty-six lactic acid bacterium strains isolated from European dairy products were identified as Streptococcus thermophilus and characterized by bacterial growth and exopolysaccharide (EPS)-producing capacity in milk and enriched milk medium. In addition, the acidification rates of the different strains were compared...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.2.900-912.2004

    authors: Vaningelgem F,Zamfir M,Mozzi F,Adriany T,Vancanneyt M,Swings J,De Vuyst L

    更新日期:2004-02-01 00:00:00

  • Induction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.

    abstract::Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nucle...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5504-5509.1999

    authors: Quirasco M,López-Munguía A,Remaud-Simeon M,Monsan P,Farrés A

    更新日期:1999-12-01 00:00:00

  • Method for detection of microorganisms that produce gaseous nitrogen oxides.

    abstract::A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.4.776-780.1986

    authors: Jenneman GE,Montgomery AD,McInerney MJ

    更新日期:1986-04-01 00:00:00

  • Clostridium acetobutylicum Protoplast Formation and Regeneration.

    abstract::Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.3.719-721.1982

    authors: Allcock ER,Reid SJ,Jones DT,Woods DR

    更新日期:1982-03-01 00:00:00

  • Effect of 3-Phenylpropanoic Acid on Capsule and Cellulases of Ruminococcus albus 8.

    abstract::The morphology and cellulases of Ruminococcus albus 8 were markedly affected by the inclusion of 3-phenylpropanoic acid (PPA) in a defined growth medium. PPA-grown bacteria produced substantial quantities of cell-bound cellulase, as well as a very high-molecular-weight extracellular enzyme and lesser amounts of two lo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.1.218-223.1984

    authors: Stack RJ,Hungate RE

    更新日期:1984-07-01 00:00:00

  • Bacterial growth stimulation with exogenous siderophore and synthetic N-acyl homoserine lactone autoinducers under iron-limited and low-nutrient conditions.

    abstract::The growth of marine bacteria under iron-limited conditions was investigated. Neither siderophore production nor bacterial growth was detected for Pelagiobacter sp. strain V0110 when Fe(III) was present in the culture medium at a concentration of <1.0 microM. However, the growth of V0110 was strongly stimulated by the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.7.2797-2803.2000

    authors: Guan LL,Onuki H,Kamino K

    更新日期:2000-07-01 00:00:00

  • Quantification of nitrogen reductase and nitrite reductase genes in soil of thinned and clear-cut Douglas-fir stands by using real-time PCR.

    abstract::The abundance of nifH, nirS, and nirK gene fragments involved in nitrogen (N) fixation and denitrification in thinned second-growth Douglas-fir (Pseudotsuga menziesii subsp. menziesii [Mirb.] Franco) forest soil was investigated by using quantitative real-time PCR. Prokaryotic N cycling is an important aspect of N ava...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02188-09

    authors: Levy-Booth DJ,Winder RS

    更新日期:2010-11-01 00:00:00

  • Detection of hepatitis A virus by hybridization with single-stranded RNA probes.

    abstract::An improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were at least eightfold ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2487-2495.1987

    authors: Jiang X,Estes MK,Metcalf TG

    更新日期:1987-10-01 00:00:00

  • Identification of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of flight mass spectrometry and artificial neural networks.

    abstract::This report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, Bacillus anthracis. Bacillus cultures were prepared ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00857-09

    authors: Lasch P,Beyer W,Nattermann H,Stämmler M,Siegbrecht E,Grunow R,Naumann D

    更新日期:2009-11-01 00:00:00

  • Isolation and properties of fungi that lyse blue-green algae.

    abstract::Of 70 pure microbial cultures isolated from aquatic habitats, soil, and air according to the ability to lyse live blue-green algae, 62 were fungi representing the genera Acremonium, Emericellopsis, and Verticillium. Algal-lysing fungi were isolated from all habitat types sampled. The remaining isolates comprised four ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.5.962-969.1978

    authors: Redhead K,Wright SJ

    更新日期:1978-05-01 00:00:00

  • Treponema saccharophilum sp. nov., a large pectinolytic spirochete from the bovine rumen.

    abstract::A large, obligately anaerobic spirochete (strain PB) was isolated from bovine rumen fluid by a procedure involving rifampin as a selective agent. The helical cells measured 0.6 to 0.7 micron by 12 to 20 micron and possessed approximately 16 periplasmic flagella inserted near each end of the protoplasmic cylinder. The ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.2.212-219.1985

    authors: Paster BJ,Canale-Parola E

    更新日期:1985-08-01 00:00:00

  • Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor.

    abstract::Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This meth...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.2.1692-1695.2006

    authors: Strovas TJ,Dragavon JM,Hankins TJ,Callis JB,Burgess LW,Lidstrom ME

    更新日期:2006-02-01 00:00:00

  • Comparative sensitivity of the echovirus type 25 JV-4 prototype strain and two recent isolates to glutaraldehyde at low concentrations.

    abstract::The sensitivity of two recently isolated antigenic variants of echovirus type 25 (Montpellier 76-1262 and Thionville 86-222) to glutaraldehyde (GTA) at low concentrations was compared with that of the JV-4 prototype strain. The purified viruses were treated under the same conditions with GTA at concentrations ranging ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.2.387-392.1994

    authors: Chambon M,Bailly JL,Peigue-Lafeuille H

    更新日期:1994-02-01 00:00:00

  • Chimeric lactase capable of spontaneous and strong immobilization on cellulose and development of a continuous-flow system for lactose hydrolysis at high temperatures.

    abstract::Recombinant plasmids containing fusion proteins composed of two different modules were constructed and expressed in Escherichia coli. The modules encoded the lactase LacA (LacZ) from the thermophilic bacterium Thermoanaerobacter ethanolicus and the cellulase CelD, a cellulose-binding module (CBM) from Anaerocellum the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01517-10

    authors: Velikodvorskaya GA,Tikhonova TV,Gurvits ID,Karyagina AS,Lavrova NV,Sergienko OV,Tashlitskii VN,Lunina NA,Lunin VG

    更新日期:2010-12-01 00:00:00

  • Fate of liberated viral RNA in wastewater determined by PCR.

    abstract::The use of a PCR method to investigate the fate of liberated Q(beta) coliphage RNA in wastewater suggests that a positive PCR result indicates the recent presence of potentially viable virus in the sample and therefore increases the public health significance of the result. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.7.2932-2933.1997

    authors: Limsawat S,Ohgaki S

    更新日期:1997-07-01 00:00:00

  • Antibacterial activity of Lactobacillus sake isolated from meat.

    abstract::A total of 221 strains of Lactobacillus isolated from meat and meat products were screened for antagonistic activities under conditions that eliminated the effects of organic acids and hydrogen peroxide. Nineteen strains of Lactobacillus sake, three strains of Lactobacillus plantarum, and one strain of Lactobacillus c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.1901-1906.1989

    authors: Schillinger U,Lücke FK

    更新日期:1989-08-01 00:00:00

  • Dense autotrophic cultures of Alcaligenes eutrophus.

    abstract::Alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled H2-O2-CO2 atmosphere. It was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. As a result...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.4.592-597.1976

    authors: Repaske R,Mayer R

    更新日期:1976-10-01 00:00:00

  • Suicide Inactivation of Catechol 2,3-Dioxygenase from Pseudomonas putida mt-2 by 3-Halocatechols.

    abstract::The inactivation of catechol 2,3-dioxygenase from Pseudomonas putida mt-2 by 3-chloro- and 3-fluorocatechol and the iron-chelating agent Tiron (catechol-3,5-disulfonate) was studied. Whereas inactivation by Tiron is an oxygen-independent and mostly reversible process, inactivation by the 3-halocatechols was only obser...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.3.500-505.1984

    authors: Bartels I,Knackmuss HJ,Reineke W

    更新日期:1984-03-01 00:00:00

  • Abundant and diverse fungal microbiota in the murine intestine.

    abstract::Enteric microbiota play a variety of roles in intestinal health and disease. While bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. This study utilized a culture-independent method termed oligonucleotide fingerprinting of rRNA genes (OFRG) to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.1.793-801.2006

    authors: Scupham AJ,Presley LL,Wei B,Bent E,Griffith N,McPherson M,Zhu F,Oluwadara O,Rao N,Braun J,Borneman J

    更新日期:2006-01-01 00:00:00

  • Anaerobic biodegradation of cyanide under methanogenic conditions.

    abstract::Upflow, anaerobic, fixed-bed, activated charcoal biotreatment columns capable of operating at free cyanide concentrations of greater than 100 mg liter-1 with a hydraulic retention time of less than 48 h were developed. Methanogenesis was maintained under a variety of feed medium conditions which included ethanol, phen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.6.1656-1662.1991

    authors: Fallon RD,Cooper DA,Speece R,Henson M

    更新日期:1991-06-01 00:00:00

  • Activation of cycasin to a mutagen for Saccharomyces cerevisiae by rat intestinal flora.

    abstract::Genetic test systems involving microorganisms and liver enzyme preparations may be insufficient to detect compounds that require breakdown by enzymes provided by the microbial flora of the intestinal tract. A method is described for providing such activation and for simultaneously testing the potential genetic activit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.2.651-657.1983

    authors: Mayer VW,Goin CJ

    更新日期:1983-02-01 00:00:00