Detection of hepatitis A virus by hybridization with single-stranded RNA probes.

Abstract:

:An improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were at least eightfold more sensitive than the 32P-labeled double-stranded cDNA counterparts, whereas biotin-labeled ssRNA probes showed a sensitivity comparable with that of the 32P-labeled double-stranded cDNA counterparts. Hybridization of HAV with the ssRNA probes at high stringency revealed specific reactions with a high signal-to-noise ratio. The differential hybridization reactions seen with probes of positive and negative sense (compared with HAV genomic RNA) were used to detect HAV in clinical and field samples. A positive/negative ratio was introduced as an indicator that permitted a semiquantitative expression of a positive HAV reaction. Good agreement of this indicator was observed with normal stool samples and with HAV-seeded samples. By using this system, HAV was detected in estuarine and freshwater samples collected from a sewage-polluted bayou in Houston and a saltwater tributary of Galveston Bay.

journal_name

Appl Environ Microbiol

authors

Jiang X,Estes MK,Metcalf TG

doi

10.1128/AEM.53.10.2487-2495.1987

subject

Has Abstract

pub_date

1987-10-01 00:00:00

pages

2487-95

issue

10

eissn

0099-2240

issn

1098-5336

journal_volume

53

pub_type

杂志文章
  • Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure.

    abstract::A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.5.1467-1472.1993

    authors: Lee C,Langlois BE,Dawson KA

    更新日期:1993-05-01 00:00:00

  • Binding of Dissolved Strontium by Micrococcus luteus.

    abstract::Resting cells of Micrococcus luteus have been shown to remove strontium (Sr) from dilute aqueous solutions of SrCl(2) at pH 7. Loadings of 25 mg of Sr per g of cell dry weight were achieved by cells exposed to a solution containing 50 ppm (mg/liter) of Sr. Sr binding occurred in the absence of nutrients and did not re...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.12.3649-3656.1990

    authors: Faison BD,Cancel CA,Lewis SN,Adler HI

    更新日期:1990-12-01 00:00:00

  • Metabolic engineering for L-glutamine overproduction by using DNA gyrase mutations in Escherichia coli.

    abstract::An L-glutamine-overproducing mutant of an Escherichia coli K-12-derived strain was selected from randomly mutagenized cells in the course of L-alanyl-L-glutamine strain development. Genome-wide mutation analysis unveiled a novel mechanism for L-glutamine overproduction in this mutant. Three mutations were identified t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03994-12

    authors: Hayashi M,Tabata K

    更新日期:2013-05-01 00:00:00

  • Nonphotosynthetic pigmented bacteria in a potable water treatment and distribution system.

    abstract::The occurrence of pigmented bacteria in potable water, from raw source water through treatment to distribution water, including dead-end locations, was compared at sample sites in a large municipal water system. Media used to enumerate heterotrophic bacteria and differentiate pigmented colonies were standard method pl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.4.912-921.1989

    authors: Reasoner DJ,Blannon JC,Geldreich EE,Barnick J

    更新日期:1989-04-01 00:00:00

  • Functional Analysis of a Novel β-(1,3)-Glucanase from Corallococcus sp. Strain EGB Containing a Fascin-Like Module.

    abstract::A novel β-(1,3)-glucanase gene designated lamC, cloned from Corallococcus sp. strain EGB, contains a fascin-like module and a glycoside hydrolase family 16 (GH16) catalytic module. LamC displays broad hydrolytic activity toward various polysaccharides. Analysis of the hydrolytic products revealed that LamC is an exo-a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01016-17

    authors: Zhou J,Li Z,Wu J,Li L,Li D,Ye X,Luo X,Huang Y,Cui Z,Cao H

    更新日期:2017-08-01 00:00:00

  • Visual evidence of horizontal gene transfer between plants and bacteria in the phytosphere of transplastomic tobacco.

    abstract::Plant surfaces, colonized by numerous and diverse bacterial species, are often considered hot spots for horizontal gene transfer (HGT) between plants and bacteria. Plant DNA released during the degradation of plant tissues can persist and remain biologically active for significant periods of time, suggesting that soil...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02632-08

    authors: Pontiroli A,Rizzi A,Simonet P,Daffonchio D,Vogel TM,Monier JM

    更新日期:2009-05-01 00:00:00

  • Effects of pH and trace minerals on long-term starvation of Leuconostoc mesenteroides.

    abstract::Laboratory experiments have definitively shown that exopolymer-producing bacteria have the potential to modify the flow of fluids in oil reservoirs to enhance oil production. Once injected into the reservoir, they will be subjected to a wide range of pH values and to starvation resulting from nutrient depletion. For s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.3.976-981.2000

    authors: Kim DS,Thomas S,Fogler HS

    更新日期:2000-03-01 00:00:00

  • Isolation and characterization of Pediococcus halophilus from salted anchovies (Engraulis anchoita).

    abstract::The presence of bacteria in salted anchovies during and at the end of the curing process was investigated. Attempts to isolate bacteria under aerobic or anaerobic conditions led to the isolation of only bacteria of the genus Pediococcus which were identified as Pediococcus halophilus. The isolates correspond to a rath...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.3.664-666.1985

    authors: Villar M,de Ruiz Holgado AP,Sanchez JJ,Trucco RE,Oliver G

    更新日期:1985-03-01 00:00:00

  • shift from acetoclastic to H2-dependent methanogenesis in a west Siberian peat bog at low pH values and isolation of an acidophilic Methanobacterium strain.

    abstract::Methane production and archaeal community composition were studied in samples from an acidic peat bog incubated at different temperatures and pH values. H(2)-dependent methanogenesis increased strongly at the lowest pH, 3.8, and Methanobacteriaceae became important except for Methanomicrobiaceae and Methanosarcinaceae...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02413-06

    authors: Kotsyurbenko OR,Friedrich MW,Simankova MV,Nozhevnikova AN,Golyshin PN,Timmis KN,Conrad R

    更新日期:2007-04-01 00:00:00

  • Mechanisms of microbial movement in subsurface materials.

    abstract::The biological factors important in the penetration of Escherichia coli through anaerobic, nutrient-saturated, Ottawa sand-packed cores were studied under static conditions. In cores saturated with galactose-peptone medium, motile strains of E. coli penetrated four times faster than mutants defective only in flagellar...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.9.2280-2286.1989

    authors: Reynolds PJ,Sharma P,Jenneman GE,McInerney MJ

    更新日期:1989-09-01 00:00:00

  • High-Level Heterologous Production of D-Cycloserine by Escherichia coli.

    abstract::Previously, we successfully cloned a d-cycloserine (d-CS) biosynthetic gene cluster consisting of 10 open reading frames (designated dcsA to dcsJ) from d-CS-producing Streptomyces lavendulae ATCC 11924. In this study, we put four d-CS biosynthetic genes (dcsC, dcsD, dcsE, and dcsG) in tandem under the control of the T...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02187-15

    authors: Kumagai T,Ozawa T,Tanimoto M,Noda M,Matoba Y,Sugiyama M

    更新日期:2015-11-01 00:00:00

  • Methodology of protistan discovery: from rRNA detection to quality scanning electron microscope images.

    abstract::Each year, thousands of new protistan 18S rRNA sequences are detected in environmental samples. Many of these sequences are molecular signatures of new protistan species, classes, and/or kingdoms that have never been seen before. The main goal of this study was to enable visualization of these novel organisms and to c...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.11.6856-6863.2003

    authors: Stoeck T,Fowle WH,Epstein SS

    更新日期:2003-11-01 00:00:00

  • Characterization of the 4-hydroxybenzoyl-coenzyme A thioesterase from Arthrobacter sp. strain SU.

    abstract::The Arthrobacter sp. strain SU 4-chlorobenzoate (4-CBA) dehalogenation pathway converts 4-CBA to 4-hydroxybenzoate (4-HBA). The pathway operon contains the genes fcbA, fcbB, and fcbC (A. Schmitz, K. H. Gartemann, J. Fiedler, E. Grund, and R. Eichenlaub, Appl. Environ. Microbiol. 58:4068-4071, 1992). Genes fcbA and fcb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.5.2707-2711.2003

    authors: Zhuang Z,Gartemann KH,Eichenlaub R,Dunaway-Mariano D

    更新日期:2003-05-01 00:00:00

  • Prevalence of bacteria of division TM7 in human subgingival plaque and their association with disease.

    abstract::Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the prese...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1687-1694.2003

    authors: Brinig MM,Lepp PW,Ouverney CC,Armitage GC,Relman DA

    更新日期:2003-03-01 00:00:00

  • Physiological adaptations involved in alkane assimilation at a low temperature by Rhodococcus sp. strain Q15.

    abstract::We examined physiological adaptations which allow the psychrotroph Rhodococcus sp. strain Q15 to assimilate alkanes at a low temperature (alkanes are contaminants which are generally insoluble and/or solid at low temperatures). During growth at 5 degrees C on hexadecane or diesel fuel, strain Q15 produced a cell surfa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.7.2961-2968.1999

    authors: Whyte LG,Slagman SJ,Pietrantonio F,Bourbonnière L,Koval SF,Lawrence JR,Inniss WE,Greer CW

    更新日期:1999-07-01 00:00:00

  • Sucrose synthesis in the nitrogen-fixing Cyanobacterium Anabaena sp. strain PCC 7120 is controlled by the two-component response regulator OrrA.

    abstract::The filamentous, nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 accumulates sucrose as a compatible solute against salt stress. Sucrose-phosphate synthase activity, which is responsible for the sucrose synthesis, is increased by salt stress, but the mechanism underlying the regulation of sucrose synthesis...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01501-14

    authors: Ehira S,Kimura S,Miyazaki S,Ohmori M

    更新日期:2014-09-01 00:00:00

  • Demethylation of Veratrole by Cytochrome P-450 in Streptomyces setonii.

    abstract::The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the beta-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.1.98-100.1986

    authors: Sutherland JB

    更新日期:1986-07-01 00:00:00

  • Species diversity of and toxin production by Gibberella fujikuroi species complex strains isolated from native prairie grasses in Kansas.

    abstract::Fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. We examined 72 Fusarium isolates from a native North American tallgrass prairie and found that Gibberella inter...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.4.2254-2262.2004

    authors: Leslie JF,Zeller KA,Logrieco A,Mulè G,Moretti A,Ritieni A

    更新日期:2004-04-01 00:00:00

  • Genetic diversity and potential function of microbial symbionts associated with newly discovered species of Osedax polychaete worms.

    abstract::We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01986-06

    authors: Goffredi SK,Johnson SB,Vrijenhoek RC

    更新日期:2007-04-01 00:00:00

  • Development and characterization of lactose-positive pediococcus species for milk fermentation.

    abstract::Bacteriophages against Streptococcus thermophilus are a growing problem in the Italian cheese industry. One possible control method involves replacing S. thermophilus in mozzarella starter blends with lactic acid bacteria from a different genus or species. In this study, we evaluated lactose-positive pediococci for th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.3.936-941.1996

    authors: Caldwell SL,McMahon DJ,Oberg CJ,Broadbent JR

    更新日期:1996-03-01 00:00:00

  • Temperature-driven adaptation of the bacterial community in peat measured by using thymidine and leucine incorporation.

    abstract::The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1116-1122.2001

    authors: Ranneklev SB,Bååth E

    更新日期:2001-03-01 00:00:00

  • Effect of phenolic acids and phenolics from plant cell walls on rumenlike fermentation in consecutive batch culture.

    abstract::Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.5.1046-1050.1987

    authors: Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

    更新日期:1987-05-01 00:00:00

  • Characterization of the isophthalate degradation genes of Comamonas sp. strain E6.

    abstract::The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01270-09

    authors: Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

    更新日期:2010-01-01 00:00:00

  • Microbial Succession of Anaerobic Chitin Degradation in Freshwater Sediments.

    abstract::Chitin is massively produced by freshwater plankton species as a structural element of their exoskeleton or cell wall. At the same time, chitin does not accumulate in the predominantly anoxic sediments, underlining its importance as carbon and nitrogen sources for sedimentary microorganisms. We studied chitin degradat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00963-19

    authors: Wörner S,Pester M

    更新日期:2019-08-29 00:00:00

  • Evaluation of two library-independent microbial source tracking methods to identify sources of fecal contamination in French estuaries.

    abstract::In order to identify the origin of the fecal contamination observed in French estuaries, two library-independent microbial source tracking (MST) methods were selected: (i) Bacteroidales host-specific 16S rRNA gene markers and (ii) F-specific RNA bacteriophage genotyping. The specificity of the Bacteroidales markers wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03003-06

    authors: Gourmelon M,Caprais MP,Ségura R,Le Mennec C,Lozach S,Piriou JY,Rincé A

    更新日期:2007-08-01 00:00:00

  • Biochemical analyses of multiple endoxylanases from the rumen bacterium Ruminococcus albus 8 and their synergistic activities with accessory hemicellulose-degrading enzymes.

    abstract::Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00353-11

    authors: Moon YH,Iakiviak M,Bauer S,Mackie RI,Cann IK

    更新日期:2011-08-01 00:00:00

  • Viability of Rhizobium bacteroids.

    abstract::Bacteroids prepared from nodules of soybean and bean were tested for viability. Contrary to the prevailing view that bacteroids are nonviable, it was found that bacteroids averaged 90% viability, irrespective of Rhizobium strain, nodule age, or nodule environment. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.6.854-856.1977

    authors: Tsien HC,Cain PS,Schmidt EL

    更新日期:1977-12-01 00:00:00

  • A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS3 Family Insertion Sequence, ISBlo11.

    abstract::Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00824-18

    authors: Sakanaka M,Nakakawaji S,Nakajima S,Fukiya S,Abe A,Saburi W,Mori H,Yokota A

    更新日期:2018-08-17 00:00:00

  • Monocentric and polycentric anaerobic fungi produce structurally related cellulases and xylanases.

    abstract::Cellulase and xylanase cDNAs were isolated from a cDNA library of the polycentric anaerobic fungus Orpinomyces sp. strain PC-2 constructed in Escherichia coli. The cellulase cDNA (celB) was 1.8 kb long with an open reading frame (ORF) coding for a polypeptide of 471 amino acids, and the xylanase cDNA (xynA) was 1.2 kb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.2.628-635.1997

    authors: Li XL,Chen H,Ljungdahl LG

    更新日期:1997-02-01 00:00:00

  • Restriction endonuclease analysis to distinguish two closely related nuclear polyhedrosis viruses: Autographa californica MNPV and Trichoplusia ni MNPV.

    abstract::Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.6.1206-1210.1978

    authors: Miller LK,Dawes KP

    更新日期:1978-06-01 00:00:00