Detection of tetracycline resistance determinants in pig isolates from three herds with different histories of antimicrobial agent exposure.

Abstract:

:A total of 114 gram-negative fecal isolates from domestic pigs in herds with different histories of antimicrobial agent exposure were screened for the presence of plasmid DNA and specific tetracycline resistance determinants. More than 84% of the isolates harbored plasmid DNA, which ranged in size from 2.1 to 186 kb. A total of 78 isolates (68.4%) were resistant to tetracycline at concentrations greater than 4 micrograms/ml. Plasmid DNAs from about 56% of the tetracycline-resistant isolates hybridized with DNA probes for class A, B, C, and D tetracycline resistance determinants. The class B determinant was the most common determinant (35% of the isolates), followed by the class C determinant (12%) and the class A determinant (1%). About 9% of the isolates contained two determinants on plasmids. None of the plasmids from isolates hybridized with the class D determinant probe. The class C determinant was the most prevalent determinant on plasmids in isolates from pigs not exposed to antimicrobial agents for more than 146 months, while the class B determinant was more prevalent on plasmids in isolates from pigs exposed to either subtherapeutic or therapeutic levels of antimicrobial agents. Most tetracycline resistance determinants were localized on plasmids which were more than 30 kb long. A great number of wild-type tetracycline-resistant Escherichia coli strains were found with the class E determinant on their chromosomes. This study revealed a high prevalence of tetracycline resistance determinants in the fecal flora of pig herds whether or not they were fed with antibiotics.

journal_name

Appl Environ Microbiol

authors

Lee C,Langlois BE,Dawson KA

doi

10.1128/AEM.59.5.1467-1472.1993

subject

Has Abstract

pub_date

1993-05-01 00:00:00

pages

1467-72

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

59

pub_type

杂志文章
  • Marine cyanophages demonstrate biogeographic patterns throughout the global ocean.

    abstract::Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02483-14

    authors: Huang S,Zhang S,Jiao N,Chen F

    更新日期:2015-01-01 00:00:00

  • Increased crystalline cellulose activity via combinations of amino acid changes in the family 9 catalytic domain and family 3c cellulose binding module of Thermobifida fusca Cel9A.

    abstract::Amino acid modifications of the Thermobifida fusca Cel9A-68 catalytic domain or carbohydrate binding module 3c (CBM3c) were combined to create enzymes with changed amino acids in both domains. Bacterial crystalline cellulose (BC) and swollen cellulose (SWC) assays of the expressed and purified enzymes showed that thre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02735-09

    authors: Li Y,Irwin DC,Wilson DB

    更新日期:2010-04-01 00:00:00

  • Evidence for interspecies gene transfer in the evolution of 2,4-dichlorophenoxyacetic acid degraders.

    abstract::Small-subunit ribosomal DNA (SSU rDNA) from 20 phenotypically distinct strains of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was partially sequenced, yielding 18 unique strains belonging to members of the alpha, beta, and gamma subgroups of the class Proteobacteria. To understand the origin of 2,4-D deg...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.4089-4092.1998

    authors: McGowan C,Fulthorpe R,Wright A,Tiedje JM

    更新日期:1998-10-01 00:00:00

  • Fungal toxicity of mobilized soil aluminum and manganese.

    abstract::Inhibition of Aspergillus flavus growth from spores was used as a simple bioassay for toxicity of Al and Mn mobilized by simulated acid precipitation. Al was identified as being toxic in soil leachates resulting from acid inputs of pH less than 2.7. Inhibition by Mn was not detectable. The addition of fluoride signifi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.46.3.758-761.1983

    authors: Firestone MK,Killham K,McColl JG

    更新日期:1983-09-01 00:00:00

  • Carbohydrate metabolism in mutants of the cyanobacterium Synechococcus elongatus PCC 7942 defective in glycogen synthesis.

    abstract::ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00397-08

    authors: Suzuki E,Ohkawa H,Moriya K,Matsubara T,Nagaike Y,Iwasaki I,Fujiwara S,Tsuzuki M,Nakamura Y

    更新日期:2010-05-01 00:00:00

  • Two types of endosymbiotic bacteria in the enigmatic marine worm Xenoturbella bocki.

    abstract::Two types of endosymbiotic bacteria were identified in the gastrodermis of the marine invertebrate Xenoturbella bocki (Xenoturbellida, Bilateria). While previously described Chlamydia-like endosymbionts were rare, Gammaproteobacteria distantly related to other endosymbionts and pathogens were abundant. The endosymbion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01092-09

    authors: Kjeldsen KU,Obst M,Nakano H,Funch P,Schramm A

    更新日期:2010-04-01 00:00:00

  • Temporal dynamics of the cecal gut microbiota of juvenile arctic ground squirrels: a strong litter effect across the first active season.

    abstract::Arctic ground squirrels (Urocitellus parryii) are active for a scant 3 to 5 months of the year. During the active season, adult squirrels compete for mates, reproduce, and fatten in preparation for hibernation, while juvenile squirrels, weaned in early July, must grow and acquire sufficient fat to survive their first ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00737-14

    authors: Stevenson TJ,Buck CL,Duddleston KN

    更新日期:2014-07-01 00:00:00

  • Autoinducer-2-producing protein LuxS, a novel salt- and chloride-induced protein in the moderately halophilic bacterium Halobacillus halophilus.

    abstract::The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01625-06

    authors: Sewald X,Saum SH,Palm P,Pfeiffer F,Oesterhelt D,Müller V

    更新日期:2007-01-01 00:00:00

  • Gene expression per gene dose, a specific measure of gene expression in aquatic microorganisms.

    abstract::A method for the measurement of specific levels of gene expression that combines target mRNA and target DNA quantitation has been developed. The use of target gene dose as a normalizing factor for mRNA provides an alternative to 16S or 23S rRNA, which are unsuitable for use in the environment because of their presence...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.2.451-457.1993

    authors: Pichard SL,Paul JH

    更新日期:1993-02-01 00:00:00

  • Bacterial community in copper sulfide ores inoculated and leached with solution from a commercial-scale copper leaching plant.

    abstract::Most copper bioleaching plants operate with a high concentration of sulfate salts caused by the continuous addition of sulfuric acid and the recycling of the leaching solution. Since the bacteria involved in bioleaching have been generally isolated at low sulfate concentrations, the bacterial population in ores leache...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.4.1344-1348.1997

    authors: Espejo RT,Romero J

    更新日期:1997-04-01 00:00:00

  • Fractionation of Stable Carbon Isotopes during Chemoautotrophic Growth of Sulfur-Oxidizing Bacteria.

    abstract::Laboratory-grown strains of chemoautotrophic Thiomicrospira sp. strain L-12 and Thiobacillus neapolitanus produced cell carbon that was 24.6 to 25.1 ppt (24.6 to 25.1 mg/g) lower in C isotope abundance than the ambient source of carbon dioxide and bicarbonate. This degree of C isotope depletion was comparable to that ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.8.1940-1943.1987

    authors: Ruby EG,Jannasch HW,Deuser WG

    更新日期:1987-08-01 00:00:00

  • Seasonal cholera caused by Vibrio cholerae serogroups O1 and O139 in the coastal aquatic environment of Bangladesh.

    abstract::Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00066-06

    authors: Alam M,Hasan NA,Sadique A,Bhuiyan NA,Ahmed KU,Nusrin S,Nair GB,Siddique AK,Sack RB,Sack DA,Huq A,Colwell RR

    更新日期:2006-06-01 00:00:00

  • An NAD(P)H-nicotine blue oxidoreductase is part of the nicotine regulon and may protect Arthrobacter nicotinovorans from oxidative stress during nicotine catabolism.

    abstract::An NAD(P)H-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of Arthrobacter nicotinovorans. Transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional acti...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02668-06

    authors: Mihasan M,Chiribau CB,Friedrich T,Artenie V,Brandsch R

    更新日期:2007-04-01 00:00:00

  • Oxygen Consumption by Desulfovibrio Strains with and without Polyglucose.

    abstract::The kinetics of oxygen reduction by Desulfovibrio salexigens Mast1 and the role of polyglucose in this activity were examined and compared with those of strains of D. desulfuricans and D. gigas. Oxidation rates were highest at air saturation (up to 40 nmol of O(2) min mg of protein) and declined with decreasing oxygen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.3.1034-1039.1998

    authors: van Niel EW,Gottschal JC

    更新日期:1998-03-01 00:00:00

  • Presence of human immunodeficiency virus nucleic acids in wastewater and their detection by polymerase chain reaction.

    abstract::The human immunodeficiency virus type 1 (HIV-1) released by infected individuals or present in human and hospital wastes can potentially cause contamination problems. The presence of HIV-1 was investigated in 16 environmental samples, including raw wastewater, sludge, final effluent, soil, and pond water, collected fr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.12.3984-3990.1992

    authors: Ansari SA,Farrah SR,Chaudhry GR

    更新日期:1992-12-01 00:00:00

  • Ammonia production by ruminal microorganisms and enumeration, isolation, and characterization of bacteria capable of growth on peptides and amino acids from the sheep rumen.

    abstract::Excessive NH(3) production in the rumen is a major nutritional inefficiency in ruminant animals. Experiments were undertaken to compare the rates of NH(3) production from different substrates in ruminal fluid in vitro and to assess the role of asaccharolytic bacteria in NH(3) production. Ruminal fluid was taken from f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.10.4925-4931.2002

    authors: Eschenlauer SC,McKain N,Walker ND,McEwan NR,Newbold CJ,Wallace RJ

    更新日期:2002-10-01 00:00:00

  • Mapping of genomic regions (quantitative trait loci) controlling production and quality in industrial cultures of the edible basidiomycete Pleurotus ostreatus.

    abstract::Industrial production of the edible basidiomycete Pleurotus ostreatus (oyster mushroom) is based on a solid fermentation process in which a limited number of selected strains are used. Optimization of industrial mushroom production depends on improving the culture process and breeding new strains with higher yields an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.6.3617-3625.2003

    authors: Larraya LM,Alfonso M,Pisabarro AG,Ramírez L

    更新日期:2003-06-01 00:00:00

  • Light as an energy source in continuous cultures of bacteriorhodopsin-containing halobacteria.

    abstract::The role of light as an energy source for slightly aereated cultures of halobacteria was studied, using continuous cultures with low nutrient concentrations and a low oxygen supply. A series of experiments were carried out with non-illuminated and differently illuminated cultures and with different oxygen transfer rat...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.45.3.868-871.1983

    authors: Rodriguez-Valera F,Nieto JJ,Ruiz-Berraquero F

    更新日期:1983-03-01 00:00:00

  • Evidence from Serpula lacrymans that 2,5-dimethoxyhydroquinone Is a lignocellulolytic agent of divergent brown rot basidiomycetes.

    abstract::Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major cause of failure in wooden structures. Recent work indicates that distinct lineages of brown rot fungi have arisen independently from ligninolytic white rot ancestors via loss of lignocellulolytic en...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03880-12

    authors: Korripally P,Timokhin VI,Houtman CJ,Mozuch MD,Hammel KE

    更新日期:2013-04-01 00:00:00

  • Cloning of a phenol oxidase gene from Acremonium murorum and its expression in Aspergillus awamori.

    abstract::Fungal multicopper oxidases have many potential industrial applications, since they perform reactions under mild conditions. We isolated a phenol oxidase from the fungus Acremonium murorum var. murorum that was capable of decolorizing plant chromophores (such as anthocyanins). This enzyme is of interest in laundry-cle...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2610-2616.2001

    authors: Gouka RJ,van der Heiden M,Swarthoff T,Verrips CT

    更新日期:2001-06-01 00:00:00

  • 3-Hydroxypyridine Dehydrogenase HpdA Is Encoded by a Novel Four-Component Gene Cluster and Catalyzes the First Step of 3-Hydroxypyridine Catabolism in Ensifer adhaerens HP1.

    abstract::3-Hydroxypyridine (3HP) is an important natural pyridine derivative. Ensifer adhaerens HP1 can utilize 3HP as its sole sources of carbon, nitrogen, and energy to grow, but the genes responsible for the degradation of 3HP remain unknown. In this study, we predicted that a gene cluster, designated 3hpd, might be respons...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01313-20

    authors: Wang H,Wang X,Ren H,Wang X,Lu Z

    更新日期:2020-09-17 00:00:00

  • Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes.

    abstract::The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.3998-4003.2000

    authors: Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

    更新日期:2000-09-01 00:00:00

  • Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates.

    abstract::Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.2924-2932.1988

    authors: Lageveen RG,Huisman GW,Preusting H,Ketelaar P,Eggink G,Witholt B

    更新日期:1988-12-01 00:00:00

  • Evidence for coexistence of distinct Escherichia coli populations in various aquatic environments and their survival in estuary water.

    abstract::Escherichia coli, a commensal bacterium from the intestinal tracts of humans and vertebrate animals, has been used as one of two bacterial indicators of fecal contamination, along with intestinal enterococci, to monitor the microbiological quality of water. However, water environments are now recognized as a secondary...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00698-13

    authors: Berthe T,Ratajczak M,Clermont O,Denamur E,Petit F

    更新日期:2013-08-01 00:00:00

  • Composition and structure of microbial communities from stromatolites of Hamelin Pool in Shark Bay, Western Australia.

    abstract::Stromatolites, organosedimentary structures formed by microbial activity, are found throughout the geological record and are important markers of biological history. More conspicuous in the past, stromatolites occur today in a few shallow marine environments, including Hamelin Pool in Shark Bay, Western Australia. Ham...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.8.4822-4832.2005

    authors: Papineau D,Walker JJ,Mojzsis SJ,Pace NR

    更新日期:2005-08-01 00:00:00

  • Rubber-degrading enzyme from a bacterial culture.

    abstract::Rubber-degrading activity was found in the extracellular culture medium of Xanthomonas sp. strain 35Y which was grown on natural rubber latex. Natural rubber in the latex state was degraded by the crude enzyme, and two fractions were separately observed by gel permeation chromatography of the reaction products. One fr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.1.269-274.1990

    authors: Tsuchii A,Takeda K

    更新日期:1990-01-01 00:00:00

  • Cloning of nod gene regions from mesquite rhizobia and bradyrhizobia and nucleotide sequence of the nodD gene from mesquite rhizobia.

    abstract::Nitrogen-fixing symbiosis between bacteria and the tree legume mesquite (Prosopis glandulosa) is important for the maintenance of many desert ecosystems. Genes essential for nodulation and for extending the host range to mesquite were isolated from cosmid libraries of Rhizobium (mesquite) sp. strain HW17b and Bradyrhi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.9.3422-3429.1995

    authors: Thomas PM,Golly KF,Virginia RA,Zyskind JW

    更新日期:1995-09-01 00:00:00

  • Interaction of Ammonia Monooxygenase from Nitrosomonas europaea with Alkanes, Alkenes, and Alkynes.

    abstract::Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.12.3187-3190.1988

    authors: Hyman MR,Murton IB,Arp DJ

    更新日期:1988-12-01 00:00:00

  • Characteristics of a Streptomyces coelicolor A3(2) extracellular protein targeting chitin and chitosan.

    abstract::Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1268-1273.2001

    authors: Saito A,Miyashita K,Biukovic G,Schrempf H

    更新日期:2001-03-01 00:00:00

  • Enhancing the secretion efficiency and thermostability of a Bacillus deramificans pullulanase mutant (D437H/D503Y) by N-terminal domain truncation.

    abstract::Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03714-14

    authors: Duan X,Wu J

    更新日期:2015-03-01 00:00:00