Demethylation of Veratrole by Cytochrome P-450 in Streptomyces setonii.

Abstract:

:The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the beta-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratrole-containing media supplemented with 2,2'-dipyridyl to inhibit cleavage of the aromatic ring, S. setonii accumulated catechol, which was detected by both liquid chromatography and gas chromatography. Reduced cell extracts from veratrole-grown cultures, but not sodium succinate-grown cultures, produced a carbon monoxide difference spectrum with a peak at 450 nm that indicated the presence of soluble cytochrome P-450. Addition of veratrole or guaiacol to oxidized cell extracts from veratrole-grown cultures produced difference spectra that indicated that these compounds were substrates for cytochrome P-450. My results suggest that S. setonii produces a cytochrome P-450 that is involved in the demethylation of veratrole and guaiacol to catechol, which is then catabolized by the beta-ketoadipate pathway.

journal_name

Appl Environ Microbiol

authors

Sutherland JB

doi

10.1128/AEM.52.1.98-100.1986

keywords:

subject

Has Abstract

pub_date

1986-07-01 00:00:00

pages

98-100

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

52

pub_type

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