Enhancement of recombinant hemoglobin production in Escherichia coli BL21(DE3) containing the Plesiomonas shigelloides heme transport system.

Abstract:

:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.

journal_name

Appl Environ Microbiol

authors

Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

doi

10.1128/AEM.01291-08

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

5854-6

issue

18

eissn

0099-2240

issn

1098-5336

pii

AEM.01291-08

journal_volume

74

pub_type

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