Molecular characterization of a gene encoding extracellular serine protease isolated from a subtilisin inhibitor-deficient mutant of Streptomyces albogriseolus S-3253.

abstract：:The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered...

journal_title：Applied and environmental microbiology

authors： Aslam M,Nattress F,Greer G,Yost C,Gill C,McMullen L

更新日期：2003-05-01 00:00:00

abstract：:The presence or absence of molecular oxygen has been shown to play a crucial role in the degradability of haloaromatic compounds. In the present study, it was shown that anaerobic phototrophic 3-chlorobenzoate (3CBA) metabolism by Rhodopseudomonas palustris DCP3 is oxygen tolerant up to a concentration of 3 microM O2....

journal_title：Applied and environmental microbiology

authors： Krooneman J,van den Akker S,Pedro Gomes TM,Forney LJ,Gottschal JC

更新日期：1999-01-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...

journal_title：Applied and environmental microbiology

authors： Zdor RE,Pueppke SG

更新日期：1988-08-01 00:00:00

abstract：:Pseudomonas pseudoalcaligenes JS52 grows on nitrobenzene via partial reduction of the nitro group and enzymatic rearrangement of the resultant hydroxylamine. Cells and cell extracts of nitrobenzene-grown JS52 catalyzed the transient formation of 4-hydroxylamino-2,6-dinitrotoluene (4HADNT), 4-amino-2,6-dinitrotoluene (...

journal_title：Applied and environmental microbiology

authors： Fiorella PD,Spain JC

更新日期：1997-05-01 00:00:00

abstract：:Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization. ...

journal_title：Applied and environmental microbiology

authors： Francis DW,Spaulding PL,Lovett J

更新日期：1980-07-01 00:00:00

abstract：:The siderophore synthetic system in Shewanella species is able to synthesize dozens of macrocyclic siderophores in vitro with synthetic precursors. In vivo, however, although three siderophores are produced naturally in Shewanella algae B516, which carries a lysine decarboxylase (AvbA) specific for siderophore synthes...

journal_title：Applied and environmental microbiology

authors： Wang S,Liang H,Liu L,Jiang X,Wu S,Gao H

更新日期：2020-03-18 00:00:00

abstract：:Plasmid constructs pNW1 through pNW6 containing a controllable xylE gene (for catechol 2,3-dioxygenase) were introduced into Streptomyces lividans strains to provide a selectable marker system. xylE functions in S. lividans under the control of bacteriophage lambda promoters lambda pL and lambda pR. Thermoregulated ex...

journal_title：Applied and environmental microbiology

authors： Wipat A,Wellington EM,Saunders VA

更新日期：1991-11-01 00:00:00

abstract：:Pectinolytic enzymes play an important role in cocoa fermentation. In this study, we characterized three extracellular pectate lyases (Pels) produced by bacilli isolated from fermenting cocoa beans. These enzymes, named Pel-22, Pel-66, and Pel-90, were synthesized by Bacillus pumilus BS22, Bacillus subtilis BS66, and ...

journal_title：Applied and environmental microbiology

authors： Ouattara HG,Reverchon S,Niamke SL,Nasser W

更新日期：2010-08-01 00:00:00

abstract：:The rapid degradation of marine infrastructure at the low tide level due to accelerated low-water corrosion (ALWC) is a problem encountered worldwide. Despite this, there is limited understanding of the microbial communities involved in this process. We obtained samples of the orange-colored tubercles commonly associa...

journal_title：Applied and environmental microbiology

authors： Phan HC,Wade SA,Blackall LL

更新日期：2020-06-17 00:00:00

abstract：:In this study, the changes in the global proteome of Salmonella in response to desiccation and thermal treatment were investigated by using an iTRAQ multiplex technique. A Salmonella enterica serovar Typhimurium strain was dried, equilibrated at high (1.0) and low (0.11) water activity (aw), and thermally treated at 7...

journal_title：Applied and environmental microbiology

authors： Maserati A,Lourenco A,Diez-Gonzalez F,Fink RC

更新日期：2018-08-31 00:00:00

abstract：:Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...

journal_title：Applied and environmental microbiology

authors： Kozhar O,Larsen MM,Grünwald NJ,Peever TL

更新日期：2020-04-17 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

journal_title：Applied and environmental microbiology

authors： George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

更新日期：2018-02-14 00:00:00

abstract：:Solid media on a base of B-12 or CB medium with agarose or agarose of low melting temperature were developed for the cultivation of Microcystis species. The media with 0.4% gel showed the highest number of CFU, and increasing the gel concentration resulted in a reduction of the number of CFU. There was no difference i...

journal_title：Applied and environmental microbiology

authors： Shirai M,Matumaru K,Ohotake A,Takamura Y,Aida T,Nakano M

更新日期：1989-10-01 00:00:00

abstract：:This study investigated the differences in microbial community abundance, composition and diversity throughout the depth profiles in soils collected from corn and soybean fields in lowa, USA using 16S rRNA amplicon sequencing. The results revealed decreased richness and diversity in microbial communities at increasing...

journal_title：Applied and environmental microbiology

authors： Hao J,Chai YN,Lopes LD,Ordóñez RA,Wright EE,Archontoulis S,Schachtman DP

更新日期：2020-12-11 00:00:00

abstract：:In Lactococcus lactis, which is widely used as a starter in the cheese industry, the first step of aromatic and branched-chain amino acid degradation is a transamination which is catalyzed by two major aminotransferases. We have previously purified and characterized biochemically and genetically the aromatic aminotran...

journal_title：Applied and environmental microbiology

authors： Yvon M,Chambellon E,Bolotin A,Roudot-Algaron F

更新日期：2000-02-01 00:00:00

abstract：:Compared to freshwater and the open ocean, less is known about bacterioplankton community structure and spatiotemporal dynamics in estuaries, particularly those with long residence times. The Chesapeake Bay is the largest estuary in the United States, but despite its ecological and economic significance, little is kno...

journal_title：Applied and environmental microbiology

authors： Kan J,Suzuki MT,Wang K,Evans SE,Chen F

更新日期：2007-11-01 00:00:00

abstract：:Lactase (beta-d-galactosidase) was produced by Candida pseudotropicalis grown in deproteinized whey. Maximum enzyme production in 2% whey was obtained by supplementation with 0.15% yeast extract, 0.1% (NH(4))(2)SO(4), and 0.05% KH(2)PO(4) (wt/vol). Highest enzyme values (4.35 U/mg of cells and 68 U/ml) were obtained w...

journal_title：Applied and environmental microbiology

authors： de Bales SA,Castillo FJ

更新日期：1979-06-01 00:00:00

abstract：:Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of ...

journal_title：Applied and environmental microbiology

authors： Higgins VJ,Braidwood M,Bell P,Bissinger P,Dawes IW,Attfield PV

更新日期：1999-02-01 00:00:00

abstract：:Recombinant DNA clones carrying high-copy or low-copy sequences from Aspergillus nidulans and Neurospora crassa were used to identify restriction fragment length polymorphisms (RFLPs) diagnostic for members of the A. flavus group: A. flavus, A. parasiticus, and A. nomius. These fungi were resolved into three distinct ...

journal_title：Applied and environmental microbiology

authors： Moody SF,Tyler BM

更新日期：1990-08-01 00:00:00

abstract：:Vibrio fischeri colonizes the squid Euprymna scolopes in a mutualistic symbiosis. Hatchling squid lack these bacterial symbionts, and V. fischeri strains must compete to occupy this privileged niche. We cloned a V. fischeri gene, designated pilA, that contributes to colonization competitiveness and encodes a protein s...

journal_title：Applied and environmental microbiology

authors： Stabb EV,Ruby EG

更新日期：2003-02-01 00:00:00

abstract：:A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

journal_title：Applied and environmental microbiology

authors： Kannisto M,Aho T,Karp M,Santala V

更新日期：2014-11-01 00:00:00

abstract：:Mortierella isabellina ATCC 38063 is a zygomycete capable of hydroxylating fish-toxic resin acids which occur in certain pulp mill effluents to nontoxic metabolites. Addition of dehydroabietic acid (1) (80 mg/liter) to a freshly inoculated culture of M. isabellina in dextrose-yeast extract broth resulted in precursor ...

journal_title：Applied and environmental microbiology

authors： Kutney JP,Choi LS,Hewitt GM,Salisbury PJ,Singh M

更新日期：1985-01-01 00:00:00

abstract：:The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594. Results confirmed that the DNA sequence of the 359-bp...

journal_title：Applied and environmental microbiology

authors： Ben-Porath J,Zehr JP

更新日期：1994-03-01 00:00:00

abstract：:Investigations concerning the role of distribution system biofilms on water quality were conducted at a drinking water utility in New Jersey. The utility experienced long-term bacteriological problems in the distribution system, while treatment plant effluents were uniformly negative for coliform bacteria. Results of ...

journal_title：Applied and environmental microbiology

authors： LeChevallier MW,Babcock TM,Lee RG

更新日期：1987-12-01 00:00:00

abstract：:Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active ...

journal_title：Applied and environmental microbiology

authors： Memmott SD,Ha YS,Dickman MB

更新日期：2002-04-01 00:00:00

abstract：:Our purpose was to develop a rapid, inexpensive method of diagnosing the source of fecal pollution in water. In previous research, we identified Bacteroides-Prevotella ribosomal DNA (rDNA) PCR markers based on analysis. These markers length heterogeneity PCR and terminal restriction fragment length polymorphism distin...

journal_title：Applied and environmental microbiology

authors： Bernhard AE,Field KG

更新日期：2000-10-01 00:00:00

abstract：:The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was pre...

journal_title：Applied and environmental microbiology

authors： Kainulainen V,Reunanen J,Hiippala K,Guglielmetti S,Vesterlund S,Palva A,Satokari R

更新日期：2013-11-01 00:00:00

abstract：:Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

journal_title：Applied and environmental microbiology

authors： Camper AK,McFeters GA

更新日期：1979-03-01 00:00:00