A PCR assay To discriminate human and ruminant feces on the basis of host differences in Bacteroides-Prevotella genes encoding 16S rRNA.

abstract：:Although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. Progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. ...

journal_title：Applied and environmental microbiology

authors： Heller D,Helmerhorst EJ,Gower AC,Siqueira WL,Paster BJ,Oppenheim FG

更新日期：2016-01-08 00:00:00

abstract：:Natural denitrification rates and activities of denitrifying enzymes were measured in an agricultural soil which had a 20-year past history of low pH (pH ca. 4) due to fertilization with acid-generating ammonium salts. The soil adjacent to this site had been limed and had a pH of ca. 6.0. Natural denitrification rates...

journal_title：Applied and environmental microbiology

authors： Parkin TB,Sexstone AJ,Tiedje JM

更新日期：1985-05-01 00:00:00

abstract：:Cells in desiccated Salmonella enterica serovar Typhimurium rdar (red, dry, and rough) morphotype colonies were examined for culturability and infectivity after 30 months. Culturability decreased only 10-fold; however, cells were underrepresented on Salmonella selective media containing bile salts. These cells were mi...

journal_title：Applied and environmental microbiology

authors： Apel D,White AP,Grassl GA,Finlay BB,Surette MG

更新日期：2009-07-01 00:00:00

abstract：:A novel β-(1,3)-glucanase gene designated lamC, cloned from Corallococcus sp. strain EGB, contains a fascin-like module and a glycoside hydrolase family 16 (GH16) catalytic module. LamC displays broad hydrolytic activity toward various polysaccharides. Analysis of the hydrolytic products revealed that LamC is an exo-a...

journal_title：Applied and environmental microbiology

authors： Zhou J,Li Z,Wu J,Li L,Li D,Ye X,Luo X,Huang Y,Cui Z,Cao H

更新日期：2017-08-01 00:00:00

abstract：:Five cases of primary amoebic meningoencephalitis associated with swimming in freshwater lakes have been recorded in Florida over the past 14 years. The present study demonstrated that pathogenic Naegleria, the causative agent, is relatively widespread. Twelve of 26 lakes sampled only once yielded the amoeba. Populati...

journal_title：Applied and environmental microbiology

authors： Wellings FM,Amuso PT,Chang SL,Lewis AL

更新日期：1977-12-01 00:00:00

abstract：:Over 1,400 water samples were collected biweekly over 6 years from an intermittent stream protected and unprotected from pasturing cattle. The samples were monitored for host-specific Bacteroidales markers, Cryptosporidium species/genotypes, viruses and coliphages associated with humans or animals, and bacterial zoono...

journal_title：Applied and environmental microbiology

authors： Wilkes G,Brassard J,Edge TA,Gannon V,Jokinen CC,Jones TH,Marti R,Neumann NF,Ruecker NJ,Sunohara M,Topp E,Lapen DR

更新日期：2013-10-01 00:00:00

abstract：:We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, s...

journal_title：Applied and environmental microbiology

authors： Rollins DM,Coolbaugh JC,Walker RI,Weiss E

更新日期：1983-01-01 00:00:00

abstract：:Oysters collected in late winter, when they were free of Vibrio vulnificus, were exposed in the organism in the laboratory. The oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. These results suggest that V. vulnif...

journal_title：Applied and environmental microbiology

authors： Kelly MT,Dinuzzo A

更新日期：1985-12-01 00:00:00

abstract：:A membrane filter procedure has been developed for the rapid quantitation of C. perfringens in the aquatic environment. Background growth is inhibited by the use of D-cycloserine, polymyxin B sulfate, and incubation at 45 degrees C. Differential characteristics include the fermentation of sucrose, production of acid p...

journal_title：Applied and environmental microbiology

authors： Bisson JW,Cabelli VJ

更新日期：1979-01-01 00:00:00

abstract：:Zoothamnium niveum is a giant, colonial marine ciliate from sulfide-rich habitats obligatorily covered with chemoautotrophic, sulfide-oxidizing bacteria which appear as coccoid rods and rods with a series of intermediate shapes. Comparative 16S rRNA gene sequence analysis and fluorescence in situ hybridization showed ...

journal_title：Applied and environmental microbiology

authors： Rinke C,Schmitz-Esser S,Stoecker K,Nussbaumer AD,Molnár DA,Vanura K,Wagner M,Horn M,Ott JA,Bright M

更新日期：2006-03-01 00:00:00

abstract：:Pseudomonas oleovorans is capable of producing poly(3-hydroxyalkanoates) (PHAs) as intracellular storage material. To analyze the possible involvement of phaD in medium-chain-length (MCL) PHA biosynthesis, we generated a phaD knockout mutant by homologous recombination. Upon disruption of the phaD gene, MCL PHA polyme...

journal_title：Applied and environmental microbiology

authors： Klinke S,de Roo G,Witholt B,Kessler B

更新日期：2000-09-01 00:00:00

abstract：:To determine rates of microbial transformation of polycyclic aromatic hydrocarbons (PAH) in freshwater sediments, C-labeled PAH were incubated with samples from both pristine and petroleum-contaminated streams. Evolved CO(2) was trapped in KOH, unaltered PAH and polar metabolic intermediate fractions were quantitated ...

journal_title：Applied and environmental microbiology

authors： Herbes SE,Schwall LR

更新日期：1978-02-01 00:00:00

abstract：:Enzymes with high specific activities at low temperatures have potential uses for chemical conversions when low temperatures are required, as in the food industry. Psychrotrophic microorganisms which grow at low temperatures may be a valuable source of cold-active enzymes that have higher activities at low temperature...

journal_title：Applied and environmental microbiology

authors： Trimbur DE,Gutshall KR,Prema P,Brenchley JE

更新日期：1994-12-01 00:00:00

abstract：:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

journal_title：Applied and environmental microbiology

authors： Jenneman GE,Montgomery AD,McInerney MJ

更新日期：1986-04-01 00:00:00

abstract：:We examined the harmful side effects on indigenous soil microorganisms of two organic solvents, acetone and dichloromethane, that are normally used for spiking of soil with polycyclic aromatic hydrocarbons for experimental purposes. The solvents were applied in two contamination protocols to either the whole soil samp...

journal_title：Applied and environmental microbiology

authors： Brinch UC,Ekelund F,Jacobsen CS

更新日期：2002-04-01 00:00:00

abstract：:A gram-negative bacterium isolated from activated sludge was able to utilize up to 25 mM phosphonoacetate as the sole carbon and phosphorus source, with simultaneous excretion of virtually equimolar levels of phosphate. 2-Aminoethylphosphonate was similarly utilized with equivalent growth rates and cellular yields, wh...

journal_title：Applied and environmental microbiology

authors： McMullan G,Harrington F,Quinn JP

更新日期：1992-04-01 00:00:00

abstract：:Studies of small noncoding RNAs (sRNAs) have been conducted predominantly using culturable organisms, and the acquisition of further information about sRNAs from global environments containing uncultured organisms now is very important. In this study, hot spring water (57°C, pH 8.1) was collected directly from the und...

journal_title：Applied and environmental microbiology

authors： Murakami S,Fujishima K,Tomita M,Kanai A

更新日期：2012-02-01 00:00:00

abstract：:Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...

journal_title：Applied and environmental microbiology

authors： Saito A,Miyashita K,Biukovic G,Schrempf H

更新日期：2001-03-01 00:00:00

abstract：:A pathway was metabolically engineered to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), a biodegradable thermoplastic with proven commercial applications, from a single, unrelated carbon source. An expression system was developed in which a prpC strain of Salmonella enterica serovar Typhimurium, with a ...

journal_title：Applied and environmental microbiology

authors： Aldor IS,Kim SW,Prather KL,Keasling JD

更新日期：2002-08-01 00:00:00

abstract：:Contamination of fresh produce with pathogenic Escherichia coli, including Shiga-toxigenic E. coli (STEC), represents a serious risk to human health. Colonization is governed by multiple bacterial and plant factors that can impact the probability and suitability of bacterial growth. Thus, we aimed to determine whether...

journal_title：Applied and environmental microbiology

authors： Merget B,Forbes KJ,Brennan F,McAteer S,Shepherd T,Strachan NJC,Holden NJ

更新日期：2019-05-16 00:00:00

abstract：:Listeria monocytogenes secretes two chitinases and one chitin binding protein. Mutants lacking chiA, chiB, or lmo2467 exhibited normal growth in cultured cells but were defective for growth in the livers and spleens of mice. Mammals lack chitin; thus, L. monocytogenes may have adapted chitinases to recognize alternati...

journal_title：Applied and environmental microbiology

authors： Chaudhuri S,Bruno JC,Alonzo F 3rd,Xayarath B,Cianciotto NP,Freitag NE

更新日期：2010-11-01 00:00:00

abstract：:Alfalfa sprouts and other seed sprouts have been implicated in numerous outbreaks of salmonellosis. The source of these epidemics appears to have been low-level contamination of seeds by Salmonella bacteria that developed into clinically significant populations during the seed germination process. To test the possibil...

journal_title：Applied and environmental microbiology

authors： Howard MB,Hutcheson SW

更新日期：2003-01-01 00:00:00

abstract：:[This corrects the article on p. 507 in vol. 30.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-12-01 00:00:00

abstract：:Resting cells of Micrococcus luteus have been shown to remove strontium (Sr) from dilute aqueous solutions of SrCl(2) at pH 7. Loadings of 25 mg of Sr per g of cell dry weight were achieved by cells exposed to a solution containing 50 ppm (mg/liter) of Sr. Sr binding occurred in the absence of nutrients and did not re...

journal_title：Applied and environmental microbiology

authors： Faison BD,Cancel CA,Lewis SN,Adler HI

更新日期：1990-12-01 00:00:00

abstract：:The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange. ...

journal_title：Applied and environmental microbiology

authors： Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RM

更新日期：2011-06-01 00:00:00

abstract：:A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from...

journal_title：Applied and environmental microbiology

authors： Reynolds D,Tranter HS,Sage R,Hambleton P

更新日期：1988-07-01 00:00:00

abstract：:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

journal_title：Applied and environmental microbiology

authors： Zhang W,Hughes J,Chen Y

更新日期：2012-06-01 00:00:00

abstract：:In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fos...

journal_title：Applied and environmental microbiology

authors： Jogler C,Lin W,Meyerdierks A,Kube M,Katzmann E,Flies C,Pan Y,Amann R,Reinhardt R,Schüler D

更新日期：2009-06-01 00:00:00

abstract：:Efficient enrichment of staphylococcal cells displaying specific heterologous affinity ligands on their cell surfaces was demonstrated by using fluorescence-activated cell sorting. Using bacterial surface display of peptide or protein libraries for the purpose of combinatorial protein engineering has previously been i...

journal_title：Applied and environmental microbiology

authors： Wernérus H,Samuelson P,Ståhl S

更新日期：2003-09-01 00:00:00

abstract：:The kinetics of the enzymatic formation of 7,8-epoxy-1-octene, 1,2-7,8-diepoxyoctane, and 1,2-epoxyoctane by growing and resting cell suspensions of Pseudomonas oleovorans are described. Formation of 1,2-epoxyoctane occurs concurrently with exponential growth on 1-octene, providing that 1-octene is in excess. Conversi...

journal_title：Applied and environmental microbiology

authors： Schwartz RD,McCoy CJ

更新日期：1976-01-01 00:00:00