Abstract:
:The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of <5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH >8.3 for flame orange.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Johansson HE,Johansson MK,Wong AC,Armstrong ES,Peterson EJ,Grant RE,Roy MA,Reddington MV,Cook RMdoi
10.1128/AEM.02289-10subject
Has Abstractpub_date
2011-06-01 00:00:00pages
4223-5issue
12eissn
0099-2240issn
1098-5336pii
AEM.02289-10journal_volume
77pub_type
杂志文章abstract::Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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