Genetic improvement of Escherichia coli for enhanced biological removal of phosphate from wastewater.

abstract：:Genetic manipulation of the category B select agents Burkholderia pseudomallei and Burkholderia mallei has been stifled due to the lack of compliant selectable markers. Hence, there is a need for additional select-agent-compliant selectable markers. We engineered a selectable marker based on the gat gene (encoding gly...

journal_title：Applied and environmental microbiology

authors： Norris MH,Kang Y,Lu D,Wilcox BA,Hoang TT

更新日期：2009-10-01 00:00:00

abstract：:Listeria app, isolated from 13 of 129 (10%) corn silage samples, 21 of 76 (28%) hay silage samples, and 3 of 5 (60%) grass silage samples during a previous Vermont survey were subjected to automated ribotype (RT) analysis. The 13 positive corn silage samples contained 3 Listeria monocytogenes isolated (three RTs, incl...

journal_title：Applied and environmental microbiology

authors： Ryser ET,Arimi SM,Donnelly CW

更新日期：1997-09-01 00:00:00

abstract：:Acinetobacter sp. strain P6 has previously been reported to utilize biphenyl (BP) and chlorinated BPs, with accumulation of corresponding chlorobenzoic acids. Arthrobacter sp. strain M5 was isolated as a contaminant in the culture of Acinetobacter sp. strain P6 growing on 4-chlorobiphenyl and showed properties similar...

journal_title：Applied and environmental microbiology

authors： Furukawa K,Chakrabarty AM

更新日期：1982-09-01 00:00:00

abstract：:Twenty-four Escherichia coli strains producing standard colicins were evaluated for inhibitory activity against 27 diarrheagenic E. coli strains of serotypes O15:H-, O26:(H11, H-), and O111:(H8, H11, H-), including O157:H7, representing diarrheagenic E. coli clones, 3, 4, 8, 9, and 10. Overlay techniques were used to ...

journal_title：Applied and environmental microbiology

authors： Murinda SE,Roberts RF,Wilson RA

更新日期：1996-09-01 00:00:00

abstract：:A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...

journal_title：Applied and environmental microbiology

authors： Heuer H,Krsek M,Baker P,Smalla K,Wellington EM

更新日期：1997-08-01 00:00:00

abstract：:The temporal and spatial distribution of Salmonella contamination in the coastal waters of Galicia (northwestern Spain) relative to contamination events with different environmental factors (temperature, wind, hours of sunlight, rainfall, and river flow) were investigated over a 4-year period. Salmonellae were isolate...

journal_title：Applied and environmental microbiology

authors： Martinez-Urtaza J,Saco M,de Novoa J,Perez-Piñeiro P,Peiteado J,Lozano-Leon A,Garcia-Martin O

更新日期：2004-04-01 00:00:00

abstract：:Using scanning electron microscopy, we observed sucker-like structures on amoebae of 13 human isolates of Naegleria fowleri. The number of suckers per amoeba seemed to vary according to the virulence of the strain. We propose the term amoebastome to describe this unique sucker-like structure of N. fowleri. ...

journal_title：Applied and environmental microbiology

authors： John DT,Cole TB Jr,Marciano-Cabral FM

更新日期：1984-01-01 00:00:00

abstract：:Peat bogs dominated by Sphagnum spp. have relatively high areal rates of dimethyl sulfide (DMS) emission to the atmosphere. DMS was produced in anoxic slurries of Sphagnum peat with a linear time course and with an average rate of 40.4 (range, 22.0 to 68.6) nmol per liter of slurry (middot) day(sup-1) observed in nine...

journal_title：Applied and environmental microbiology

authors： Kiene RP,Hines ME

更新日期：1995-07-01 00:00:00

abstract：:Thermoanaerobacter sp. strain X514 has great potential in biotechnology due to its capacity to ferment a range of C(5) and C(6) sugars to ethanol and other metabolites under thermophilic conditions. This study investigated the central metabolism of strain X514 via (13)C-labeled tracer experiments using either glucose ...

journal_title：Applied and environmental microbiology

authors： Feng X,Mouttaki H,Lin L,Huang R,Wu B,Hemme CL,He Z,Zhang B,Hicks LM,Xu J,Zhou J,Tang YJ

更新日期：2009-08-01 00:00:00

abstract：:Copper resistance mechanisms provide an important adaptive advantage to plant pathogenic bacteria under exposure to copper treatments. Copper resistance determinants have been described in Pseudomonas syringae pv. syringae (Pss) strains isolated from mango intimately associated with 62 kb plasmids belonging to the pPT...

journal_title：Applied and environmental microbiology

authors： Aprile F,Heredia-Ponce Z,Cazorla FM,de Vicente A,Gutiérrez-Barranquero JA

更新日期：2020-12-23 00:00:00

abstract：:A sublethal dose of ethanol (5%, vol/vol), acid (HCl, pH 4.5 to 5.0), H2O2 (500 ppm), or NaCl (7%, wt/vol) was added to a Listeria monocytogenes culture at the exponential phase, and the cells were allowed to grow for 1 h. Exponential-phase cells also were heat shocked at 45 degrees C for 1 h. The stress-adapted cells...

journal_title：Applied and environmental microbiology

authors： Lou Y,Yousef AE

更新日期：1997-04-01 00:00:00

abstract：:Recent outbreaks linked to Salmonella-contaminated produce heightened the need to develop simple, rapid, and accurate detection methods, particularly those capable of determining cell viability. In this study, we examined a novel strategy for the rapid detection and quantification of viable salmonellae in produce by c...

journal_title：Applied and environmental microbiology

authors： Chen S,Wang F,Beaulieu JC,Stein RE,Ge B

更新日期：2011-06-01 00:00:00

abstract：:The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...

journal_title：Applied and environmental microbiology

authors： Aranda-Olmedo I,Marín P,Ramos JL,Marqués S

更新日期：2006-11-01 00:00:00

abstract：:Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with...

journal_title：Applied and environmental microbiology

authors： Orrison LH,Cherry WB,Fliermans CB,Dees SB,McDougal LK,Dodd DJ

更新日期：1981-07-01 00:00:00

abstract：:The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli Recombinant PcAxy43A consisting of a glycoside hydrolase family 43 and a family 6 carbohydrate-binding module exhibited endo-xylanase, β-xylosidase, and arabin...

journal_title：Applied and environmental microbiology

authors： Teeravivattanakit T,Baramee S,Phitsuwan P,Waeonukul R,Pason P,Tachaapaikoon C,Sakka K,Ratanakhanokchai K

更新日期：2016-12-01 00:00:00

abstract：:Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in...

journal_title：Applied and environmental microbiology

authors： Wegkamp A,Starrenburg M,de Vos WM,Hugenholtz J,Sybesma W

更新日期：2004-05-01 00:00:00

abstract：:A total of 765 Escherichia coli isolates from point and nonpoint sources were collected from the Apalachicola National Estuarine Research Reserve, and their multiple-antibiotic-resistance (MAR) profiles were determined with 10 antibiotics. E. coli isolates from point sources showed significantly greater resistance (P ...

journal_title：Applied and environmental microbiology

authors： Parveen S,Murphree RL,Edmiston L,Kaspar CW,Portier KM,Tamplin ML

更新日期：1997-07-01 00:00:00

abstract：:Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

journal_title：Applied and environmental microbiology

authors： Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

更新日期：2013-03-01 00:00:00

abstract：:The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granule-associated protein that protects recombinant Escherichia coli against several kinds of...

journal_title：Applied and environmental microbiology

authors： Mezzina MP,Álvarez DS,Egoburo DE,Díaz Peña R,Nikel PI,Pettinari MJ

更新日期：2017-06-30 00:00:00

abstract：:Three bacteria capable of utilizing bis(4-hydroxyphenyl)methane (bisphenol F [BPF]) as the sole carbon source were isolated from river water, and they all belonged to the family Sphingomonadaceae. One of the isolates, designated Sphingobium yanoikuyae strain FM-2, at an initial cell density of 0.01 (optical density at...

journal_title：Applied and environmental microbiology

authors： Inoue D,Hara S,Kashihara M,Murai Y,Danzl E,Sei K,Tsunoi S,Fujita M,Ike M

更新日期：2008-01-01 00:00:00

abstract：:The utilization of gels, which are used for fluid drilling of seeds, as carriers of Bradyrhizobium japonicum for soybean (Glycine max (L.) Merr.) inoculation was studied. Gels of various chemical composition (magnesium silicate, potassium acrylate-acrylamide, grafted starch, and hydroxyethyl cellulose) were used, alth...

journal_title：Applied and environmental microbiology

authors： Jawson MD,Franzluebbers AJ,Berg RK

更新日期：1989-03-01 00:00:00

abstract：:Previous studies have shown that the biodegradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 proceeds by the reduction of nitrobenzene through nitrosobenzene and hydroxylaminobenzene, followed by rearrangement to 2-aminophenol, which then undergoes meta ring cleavage. We report here the isolation of a Com...

journal_title：Applied and environmental microbiology

authors： Nishino SF,Spain JC

更新日期：1995-06-01 00:00:00

abstract：:Degradation of cotton cellulose by Trichoderma reesei endoglucanase I (EGI) and cellobiohydrolase II (CBHII) was investigated by analyzing the insoluble cellulose fragments remaining after enzymatic hydrolysis. Changes in the molecular-size distribution of cellulose after attack by EGI, alone and in combination with C...

journal_title：Applied and environmental microbiology

authors： Kleman-Leyer KM,Siika-Aho M,Teeri TT,Kirk TK

更新日期：1996-08-01 00:00:00

abstract：:The development of resistance in foodborne pathogens to food preservation techniques is an issue of increasing concern, especially in minimally processed foods where safety relies on hurdle technology. In this context, mild heat can be used in combination with so-called nonthermal processes, such as high hydrostatic p...

journal_title：Applied and environmental microbiology

authors： Gayán E,Cambré A,Michiels CW,Aertsen A

更新日期：2016-10-27 00:00:00

abstract：:It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences...

journal_title：Applied and environmental microbiology

authors： Liu Z,Ma P,Holtsmark I,Skaugen M,Eijsink VG,Brurberg MB

更新日期：2013-09-01 00:00:00

abstract：:Shewanella oneidensis couples oxidation of lactate to respiration of many substrates. Here we report that llpR (L-lactate-positive regulator, SO_3460) encodes a positive regulator of L-lactate utilization distinct from previously studied regulators. We also demonstrate D-lactate inhibition of L-lactate utilization in ...

journal_title：Applied and environmental microbiology

authors： Brutinel ED,Gralnick JA

更新日期：2012-12-01 00:00:00

abstract：:Algal-virus-specific PCR primers were used to amplify DNA polymerase gene (pol) fragments (683 to 689 bp) from the virus-sized fraction (0.02 to 0.2 microns) concentrated from inshore and offshore water samples collected from the Gulf of Mexico. Algal-virus-like DNA pol genes were detected in five samples collected fr...

journal_title：Applied and environmental microbiology

authors： Chen F,Suttle CA,Short SM

更新日期：1996-08-01 00:00:00

abstract：:Geobacter sulfurreducens PCA completely oxidized lactate and reduced iron or an electrode, producing pyruvate and acetate intermediates. Compared to the current produced by Shewanella oneidensis MR-1, G. sulfurreducens PCA produced 10-times-higher current levels in lactate-fed microbial electrolysis cells. The kinetic...

journal_title：Applied and environmental microbiology

authors： Call DF,Logan BE

更新日期：2011-12-01 00:00:00

abstract：:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...

journal_title：Applied and environmental microbiology

authors： De Oliveira VC,Maeda I,Delessert S,Poirier Y

更新日期：2004-09-01 00:00:00

abstract：:A clinical isolate of Pseudomonas aeruginosa was found capable of utilizing salicylate by the salicylate hydroxylase and beta-ketoadipate pathway. ...

journal_title：Applied and environmental microbiology

authors： Ohta S,Matsumoto H,Terawaki Y

更新日期：1981-01-01 00:00:00