Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes.

abstract：:Spiroplasma citri is transmitted from plant to plant by phloem-feeding leafhoppers. In an attempt to identify mechanisms involved in transmission, mutants of S. citri affected in their transmission must be available. For this purpose, transposon (Tn4001) mutagenesis was used to produce mutants which have been screened...

journal_title：Applied and environmental microbiology

authors： Boutareaud A,Danet JL,Garnier M,Saillard C

更新日期：2004-07-01 00:00:00

abstract：:We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...

journal_title：Applied and environmental microbiology

authors： Liu J,Xie Z,Merritt J,Qi F

更新日期：2012-05-01 00:00:00

abstract：:Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...

journal_title：Applied and environmental microbiology

authors： Saito A,Miyashita K,Biukovic G,Schrempf H

更新日期：2001-03-01 00:00:00

abstract：:The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB

更新日期：1997-05-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:Microbial mercury methylation and methylmercury decomposition were examined in Lake Clara, an oligotrophic northern Wisconsin seepage lake, using radioisotopic tracers. Methylation activity was near background in the water column, was greatest in the profundal surficial sediments, and decreased with depth in sediment ...

journal_title：Applied and environmental microbiology

authors： Korthals ET,Winfrey MR

更新日期：1987-10-01 00:00:00

abstract：:Protein S is a major spore coat protein of Myxococcus xanthus, consisting of two homologous domains, the N-terminal domain (NTD) and the C-terminal domain, both of which contain a Ca(2+)-binding site. Protein S tightly binds to myxospores in a Ca(2+)-dependent manner. Here, we constructed a novel expression vector, pC...

journal_title：Applied and environmental microbiology

authors： Kobayashi H,Yoshida T,Inouye M

更新日期：2009-08-01 00:00:00

abstract：:This work presents the characterization of an uptake hydrogenase from a marine filamentous nonheterocystous cyanobacterium, Lyngbya majuscula CCAP 1446/4. The structural genes encoding the uptake hydrogenase (hupSL) were isolated and characterized, and regulatory sequences were identified upstream of hupS. In silico a...

journal_title：Applied and environmental microbiology

authors： Leitão E,Oxelfelt F,Oliveira P,Moradas-Ferreira P,Tamagnini P

更新日期：2005-08-01 00:00:00

abstract：:A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...

journal_title：Applied and environmental microbiology

authors： Mooney A,O'Leary ND,Dobson AD

更新日期：2006-02-01 00:00:00

abstract：:We studied the physiological effect of the interconversion between the NAD(H) and NADP(H) coenzyme systems in recombinant Saccharomyces cerevisiae expressing the membrane-bound transhydrogenase from Escherichia coli. Our objective was to determine if the membrane-bound transhydrogenase could work in reoxidation of NAD...

journal_title：Applied and environmental microbiology

authors： Anderlund M,Nissen TL,Nielsen J,Villadsen J,Rydström J,Hahn-Hägerdal B,Kielland-Brandt MC

更新日期：1999-06-01 00:00:00

abstract：:Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

journal_title：Applied and environmental microbiology

authors： Bender J,Vatcharapijarn Y,Jeffries TW

更新日期：1985-03-01 00:00:00

abstract：:The actinomycete Streptomyces setonii 75Vi2 demethylates vanillic acid and guaiacol to protocatechuic acid and catechol, respectively, and then metabolizes the products by the beta-ketoadipate pathway. UV spectroscopy showed that this strain could also metabolize veratrole (1,2-dimethoxybenzene). When grown in veratro...

journal_title：Applied and environmental microbiology

authors： Sutherland JB

更新日期：1986-07-01 00:00:00

abstract：:Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. ...

journal_title：Applied and environmental microbiology

authors： Corbett LL,Parker DL

更新日期：1976-12-01 00:00:00

abstract：:In all purification stages of a biological sewage treatment plant, phototrophic bacteria were detected by the method of viable cell counts. The predominant species identified belonged to the genus Rhodopseudomonas of purple nonsulfur bacteria. The number of phototrophic bacteria was highest in wastewater containing sl...

journal_title：Applied and environmental microbiology

authors： Siefert E,Irgens RL,Pfennig N

更新日期：1978-01-01 00:00:00

abstract：:Escherichia coli O157:H7 is a leading cause of food-borne illness. This human pathogen produces Shiga toxins (Stx1 and Stx2) which inhibit protein synthesis by inactivating ribosome function. The present study describes a novel cell-based assay to detect Stx2 and inhibitors of toxin activity. A Vero cell line harborin...

journal_title：Applied and environmental microbiology

authors： Quiñones B,Massey S,Friedman M,Swimley MS,Teter K

更新日期：2009-03-01 00:00:00

abstract：:The subsurface of a tidal-flat sediment was analyzed down to 360 cm in depth by molecular and geochemical methods. A community structure analysis of all three domains of life was performed using domain-specific PCR followed by denaturing gradient gel electrophoresis analysis and sequencing of characteristic bands. The...

journal_title：Applied and environmental microbiology

authors： Wilms R,Sass H,Köpke B,Köster J,Cypionka H,Engelen B

更新日期：2006-04-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:The accurate description of a microbial community is an important first step in understanding the roles of its components in ecosystem function. A method for surveying microbial communities termed serial analysis of rRNA genes (SARD) is described here. Through a series of molecular cloning steps, short DNA sequence ta...

journal_title：Applied and environmental microbiology

authors： Ashby MN,Rine J,Mongodin EF,Nelson KE,Dimster-Denk D

更新日期：2007-07-01 00:00:00

abstract：:Studies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of...

journal_title：Applied and environmental microbiology

authors： Powell JE,Martinson VG,Urban-Mead K,Moran NA

更新日期：2014-12-01 00:00:00

abstract：:With a medium containing citrate as the carbon and energy source, 10 clostridial strains were isolated from various mud samples. Characterization of these strains revealed that they all belonged to the same species, Clostridium sphenoides. Strains of this organism obtained from culture collections were also able to gr...

journal_title：Applied and environmental microbiology

authors： Walther R,Hippe H,Gottschalk G

更新日期：1977-04-01 00:00:00

abstract：:A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occurring in the upper outflow channel (temperature, 82 to 88 degreesC) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89 degreesC in the neutral to...

journal_title：Applied and environmental microbiology

authors： Huber R,Eder W,Heldwein S,Wanner G,Huber H,Rachel R,Stetter KO

更新日期：1998-10-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) is a significant zoonotic pathogen causing severe disease associated with watery and bloody diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome (HUS) in humans. Infections are frequently associated with contact with EHEC-contaminated ruminant feces. Both natural a...

journal_title：Applied and environmental microbiology

authors： Joris MA,Vanrompay D,Verstraete K,De Reu K,De Zutter L,Cox E

更新日期：2013-06-01 00:00:00

abstract：:Mucoromycota representatives are known to harbour two types of endohyphal bacteria (EHB) - Burkholderia related endobacteria (BRE) and Mycoplasma related endobacteria (MRE). While both BRE and MRE occur in fungi representing all subphyla of Mucoromycota, their distribution is not well studied. Therefore, it is difficu...

journal_title：Applied and environmental microbiology

authors： Okrasińska A,Bokus A,Duk K,Gęsiorska A,Sokołowska B,Miłobędzka A,Wrzosek M,Pawłowska J

更新日期：2021-01-22 00:00:00

abstract：:[This corrects the article on p. 4430 in vol. 63.]. ...

journal_title：Applied and environmental microbiology

authors： Restrepo S,Verdier V

更新日期：1998-03-01 00:00:00

abstract：:Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and...

journal_title：Applied and environmental microbiology

authors： Wallis PM,Wallis HM

更新日期：1986-03-01 00:00:00

abstract：:Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chicken...

journal_title：Applied and environmental microbiology

authors： Nothaft H,Perez-Muñoz ME,Gouveia GJ,Duar RM,Wanford JJ,Lango-Scholey L,Panagos CG,Srithayakumar V,Plastow GS,Coros C,Bayliss CD,Edison AS,Walter J,Szymanski CM

更新日期：2017-11-16 00:00:00

abstract：:Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for divers...

journal_title：Applied and environmental microbiology

authors： Fogg PCM,Haley JA,Stark WM,Smith MCM

更新日期：2017-02-15 00:00:00

abstract：:Cadmium is an important heavy metal pollutant. For this study, we investigated the effects of cadmium exposure on the oxidative stress responses of Xanthomonas campestris, a soil and plant pathogenic bacterium. The exposure of X. campestris to low concentrations of cadmium induces cross-protection against subsequent k...

journal_title：Applied and environmental microbiology

authors： Banjerdkij P,Vattanaviboon P,Mongkolsuk S

更新日期：2005-04-01 00:00:00

abstract：:Spatial and temporal variations in sediment microbial community structure in a eutrophic lake polluted with inorganic mercury were identified using polar lipid fatty acid (PLFA) analysis. Microbial community structure was strongly related to mercury methylation potential, sediment organic carbon content, and lake loca...

journal_title：Applied and environmental microbiology

authors： Macalady JL,Mack EE,Nelson DC,Scow KM

更新日期：2000-04-01 00:00:00

abstract：:A simple, automated microplate system for biochemical characterization of water isolates can be used to obtain fingerprints of the bacterial flora from various water samples. Mathematical models for calculating the diversities and similarities between bacterial populations are described for such fingerprints. The dive...

journal_title：Applied and environmental microbiology

authors： Kühn I,Allestam G,Stenström TA,Möllby R

更新日期：1991-11-01 00:00:00