Abstract:
:It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Liu Z,Ma P,Holtsmark I,Skaugen M,Eijsink VG,Brurberg MBdoi
10.1128/AEM.01065-13subject
Has Abstractpub_date
2013-09-01 00:00:00pages
5721-7issue
18eissn
0099-2240issn
1098-5336pii
AEM.01065-13journal_volume
79pub_type
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