New type of antimicrobial protein produced by the plant pathogen Clavibacter michiganensis subsp. michiganensis.

Abstract:

:It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease.

journal_name

Appl Environ Microbiol

authors

Liu Z,Ma P,Holtsmark I,Skaugen M,Eijsink VG,Brurberg MB

doi

10.1128/AEM.01065-13

subject

Has Abstract

pub_date

2013-09-01 00:00:00

pages

5721-7

issue

18

eissn

0099-2240

issn

1098-5336

pii

AEM.01065-13

journal_volume

79

pub_type

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