Ice Nucleation Activity in Fusarium acuminatum and Fusarium avenaceum.

abstract：:Clostridium difficile causes a potentially fatal diarrheal disease through the production of its principal virulence factors, toxin A and toxin B. The tcdC gene is thought to encode a negative regulator of toxin production. Therefore, increased toxin production, and hence increased virulence, is often inferred in stra...

journal_title：Applied and environmental microbiology

authors： Cartman ST,Kelly ML,Heeg D,Heap JT,Minton NP

更新日期：2012-07-01 00:00:00

abstract：:An assay has been developed to detect bacterial glycosidases in colonies grown on the surface of agar plates. Advantages of this technique over previously described methods include elimination of the need for replica plating, better visualization of chromagenic reaction products, and a simple permeabilization step to ...

journal_title：Applied and environmental microbiology

authors： Paoni NF,Arroyo RL

更新日期：1984-01-01 00:00:00

abstract：:To study the impact of solar UV radiation (UVR) (280 to 400 nm) on the filamentous cyanobacterium Arthrospira (Spirulina) platensis, we examined the morphological changes and photosynthetic performance using an indoor-grown strain (which had not been exposed to sunlight for decades) and an outdoor-grown strain (which ...

journal_title：Applied and environmental microbiology

authors： Wu H,Gao K,Villafañe VE,Watanabe T,Helbling EW

更新日期：2005-09-01 00:00:00

abstract：:Insecticidal crystal proteins from Bacillus thuringiensis in sprays and transgenic crops are extremely useful for environmentally sound pest management, but their long-term efficacy is threatened by evolution of resistance by target pests. The diamondback moth (Plutella xylostella) is the first insect to evolve resist...

journal_title：Applied and environmental microbiology

authors： Ballester V,Granero F,Tabashnik BE,Malvar T,Ferré J

更新日期：1999-04-01 00:00:00

abstract：:Fusarium fungal contaminants and related mycotoxins were investigated in eight maize feed samples submitted to the Iowa State University Veterinary Diagnostic Laboratory. Fusarium moniliforme, F. proliferatum, and F. subglutinans were isolated from seven, eight, and five samples, respectively. These strains belonged t...

journal_title：Applied and environmental microbiology

authors： Munkvold G,Stahr HM,Logrieco A,Moretti A,Ritieni A

更新日期：1998-10-01 00:00:00

abstract：:A sedimentary bacterial isolate has been shown to contain trans-monounsaturated fatty acids (6% of the total fatty acids). The ratio of trans- to cis-acids in this isolate was in the range 3.2 to 7.6. The identification of trans-monounsaturated acids in a marine bacterium implied that the trans-acids which have been r...

journal_title：Applied and environmental microbiology

authors： Gillan FT,Johns RB,Verheyen TV,Volkman JK,Bavor HJ

更新日期：1981-04-01 00:00:00

abstract：:Sakacin A is an antilisterial bacteriocin produced by Lactobacillus sake Lb706. In order to identify genes involved in sakacin A production and immunity, the plasmid fraction of L. sake Lb706 was shotgun cloned directly into a sakacin A-nonproducing and -sensitive variant, L. sake Lb706-B, by using the broad-host-rang...

journal_title：Applied and environmental microbiology

authors： Axelsson L,Holck A,Birkeland SE,Aukrust T,Blom H

更新日期：1993-09-01 00:00:00

abstract：:Two mouse immunoglobulin G1 monoclonal antibodies that bind to the trichothecene mycotoxin T-2 were prepared. These antibodies, designated 12C12 and 15H6, had affinities for T-2 of 3.5 X 10(6) and 5.8 X 10(7) liters/mol, respectively. A competitive inhibition enzyme immunoassay that employed these antibodies had a sen...

journal_title：Applied and environmental microbiology

authors： Hunter KW Jr,Brimfield AA,Miller M,Finkelman FD,Chu SF

更新日期：1985-01-01 00:00:00

abstract：:Legionella pneumophila was detected and identified by an immunoblot assay using a monoclonal antibody specific to serogroups 1 to 8. Samples containing L. pneumophila were plated on buffered charcoal yeast extract agar supplemented with glycine, vancomycin, and polymyxin B. After incubation at 35 degrees C for 3 days,...

journal_title：Applied and environmental microbiology

authors： Bérubé A,Trudel M,Payment P

更新日期：1989-06-01 00:00:00

abstract：:The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, a...

journal_title：Applied and environmental microbiology

authors： Kurz M,Burch AY,Seip B,Lindow SE,Gross H

更新日期：2010-08-01 00:00:00

abstract：:The heat resistance of susceptible and multiantimicrobial-resistant Salmonella strains grown to stationary phase in glucose-free tryptic soy broth supplemented with 0.6% yeast extract (TSBYE-G; nonadapted), in regular (0.25% glucose) TSBYE, or in TSBYE-G with 1.00% added glucose (TSBYE+G; acid adapted) was determined ...

journal_title：Applied and environmental microbiology

authors： Bacon RT,Ransom JR,Sofos JN,Kendall PA,Belk KE,Smith GC

更新日期：2003-07-01 00:00:00

abstract：:We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subcult...

journal_title：Applied and environmental microbiology

authors： Jin YS,Alper H,Yang YT,Stephanopoulos G

更新日期：2005-12-01 00:00:00

abstract：:Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance of the plasmid. One is a minimum replicon including one gene, repT, coding a replication initiator, and the other encodes subunits of class I ribonucleotide redu...

journal_title：Applied and environmental microbiology

authors： Ohtani N,Tomita M,Itaya M

更新日期：2015-12-28 00:00:00

abstract：:A novel display system is described that allows highly efficient immobilization of heterologous proteins on bacterial surfaces in applications for which the use of genetically modified bacteria is less desirable. This system is based on nonliving and non-genetically modified gram-positive bacterial cells, designated g...

journal_title：Applied and environmental microbiology

authors： Bosma T,Kanninga R,Neef J,Audouy SA,van Roosmalen ML,Steen A,Buist G,Kok J,Kuipers OP,Robillard G,Leenhouts K

更新日期：2006-01-01 00:00:00

abstract：:Interactions of soybean with Bradyrhizobium japonicum 123 (serogroup 123) and 138 (serogroup c1) were used to examine the relationship between early infection rates, competition for nodulation, and patterns of nodule occupancy. Both strains formed more infections in autoclaved soil (sterile soil) than in untreated soi...

journal_title：Applied and environmental microbiology

authors： Zdor RE,Pueppke SG

更新日期：1988-08-01 00:00:00

abstract：:An aminotransferase capable of transaminating 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid to L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG), was purified to apparent homogeneity from Escherichia coli K-12. The enzyme catalyzes the trans...

journal_title：Applied and environmental microbiology

authors： Schulz A,Taggeselle P,Tripier D,Bartsch K

更新日期：1990-01-01 00:00:00

abstract：:From Bradyrhizobium japonicum highly reiterated sequence-possessing (HRS) strains indigenous to Niigata and Tokachi in Japan with high copy numbers of the repeated sequences RSalpha and RSbeta (K. Minamisawa, T. Isawa, Y. Nakatsuka, and N. Ichikawa, Appl. Environ. Microbiol. 64:1845-1851, 1998), several insertion sequ...

journal_title：Applied and environmental microbiology

authors： Isawa T,Sameshima R,Mitsui H,Minamisawa K

更新日期：1999-08-01 00:00:00

abstract：:Fifteen Bacillus thuringiensis strains representing 13 serotypes were screened with five oligodeoxyribonucleotide probes specific for certain regions of two published sequences and one unpublished sequence of B. thuringiensis delta-endotoxin genes. Of the 15 cultures, 14 hybridized with at least one probe; the B. thur...

journal_title：Applied and environmental microbiology

authors： Prefontaine G,Fast P,Lau PC,Hefford MA,Hanna Z,Brousseau R

更新日期：1987-12-01 00:00:00

abstract：:The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

journal_title：Applied and environmental microbiology

authors： Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

更新日期：2010-01-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:Crenarchaeol, a membrane-spanning glycerol dialkyl glycerol tetraether (GDGT) containing a cyclohexane moiety in addition to four cyclopentane moieties, was originally hypothesized to be synthesized exclusively by the mesophilic Crenarchaeota. Recent studies reporting the occurrence of crenarchaeol in hot springs and ...

journal_title：Applied and environmental microbiology

authors： Pitcher A,Schouten S,Sinninghe Damsté JS

更新日期：2009-07-01 00:00:00

abstract：UNLABELLED:In the summer of 2010, Vibrio parahaemolyticus caused an outbreak in Maryland linked to the consumption of oysters. Strains isolated from both stool and oyster samples were indistinguishable by pulsed-field gel electrophoresis (PFGE). However, the oysters contained other potentially pathogenic V. parahaemoly...

journal_title：Applied and environmental microbiology

authors： Haendiges J,Jones J,Myers RA,Mitchell CS,Butler E,Toro M,Gonzalez-Escalona N

更新日期：2016-05-16 00:00:00

abstract：:Anti-Helicobacter pylori activities were determined by agar dilution, confocal laser scanning microscopy, and cell proliferation assays following treatment with various grape extracts. Muscadine grape skin possessed the strongest activity, followed by grape synergy (skin and seed) and seed, suggesting that higher phen...

journal_title：Applied and environmental microbiology

authors： Brown JC,Huang G,Haley-Zitlin V,Jiang X

更新日期：2009-02-01 00:00:00

abstract：:Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic r...

journal_title：Applied and environmental microbiology

authors： Scholz BK,Jakobek JL,Lindgren PB

更新日期：1994-04-01 00:00:00

abstract：:A homolog of the clpC ATPase gene was identified in the genome of Bifidobacterium breve UCC 2003. Since this gene is very well conserved among eubacteria, we employed a PCR-based approach using primers based on highly conserved regions of ClpC proteins in order to identify homologous genes in other bifidobacterial spe...

journal_title：Applied and environmental microbiology

authors： Ventura M,Fitzgerald GF,van Sinderen D

更新日期：2005-10-01 00:00:00

abstract：:Carnobacteriocin B2 is a well-characterized class II bacteriocin produced by a 61-kb plasmid from Carnobacterium piscicola LV17. Export of this bacteriocin is dependent on specific ABC (ATP-binding cassette) secretion proteins. Divergicin A is a strongly hydrophobic narrow-spectrum bacteriocin produced by a 3.4-kb pla...

journal_title：Applied and environmental microbiology

authors： McCormick JK,Worobo RW,Stiles ME

更新日期：1996-11-01 00:00:00

abstract：:The effect of the washing aid T-128 (generally recognized as safe [GRAS] formulation, composed mainly of phosphoric acid and propylene glycol) on inactivation of Salmonella and Pseudomonas populations in biofilms on stainless steel was evaluated under conditions of increasing organic matter loads in chlorinated wash s...

journal_title：Applied and environmental microbiology

authors： Shen C,Luo Y,Nou X,Bauchan G,Zhou B,Wang Q,Millner P

更新日期：2012-10-01 00:00:00

abstract：:A homokaryotic, clavine alkaloid-producing strain of ergot, Claviceps sp. strain SD 58, was used in an attempt to demonstrate parasexuality. Genetically marked auxotrophic strains were produced by mutation with N-methyl-N'-nitro-N-nitrosoguanidine. Protoplast fusion of pairs of unlike doubly auxotrophic strains and is...

journal_title：Applied and environmental microbiology

authors： Brauer KL,Robbers JE

更新日期：1987-01-01 00:00:00

abstract：:Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fo...

journal_title：Applied and environmental microbiology

authors： Hemelt DM,Mares B,Upadhyay JM

更新日期：1979-09-01 00:00:00

abstract：:A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions O...

journal_title：Applied and environmental microbiology

authors： Miyoshi T,Iwatsuki T,Naganuma T

更新日期：2005-02-01 00:00:00