Improved method for detection of glycosidases in bacterial colonies.

abstract：:Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...

journal_title：Applied and environmental microbiology

authors： Mallison SM 3rd,Cannon RE

更新日期：1984-05-01 00:00:00

abstract：:Single 10-bp primers were used to generate random amplified polymorphic DNA (RAPD) markers from commercial and wild strains of the cultivated mushroom Agaricus bisporus via the polymerase chain reaction. Of 20 primers tested, 19 amplified A. bisporus DNA, each producing 5 to 15 scorable markers ranging from 0.5 to 3.0...

journal_title：Applied and environmental microbiology

authors： Khush RS,Becker E,Wach M

更新日期：1992-09-01 00:00:00

abstract：:We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background bu...

journal_title：Applied and environmental microbiology

authors： Charles TC,Doty SL,Nester EW

更新日期：1994-11-01 00:00:00

abstract：:The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

journal_title：Applied and environmental microbiology

authors： Lee LW,Chiou CH,Linz JE

更新日期：2002-11-01 00:00:00

abstract：:A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of prot...

journal_title：Applied and environmental microbiology

authors： Jackman DM,Bartlett FM,Patel TR

更新日期：1983-07-01 00:00:00

abstract：:A separation procedure for the analysis of the enzyme components of the hyperthermophilic bacterium Thermotoga maritima involved in cellulose and xylan degradation was developed. Resolution of the enzymes was achieved by a combination of fast protein liquid chromatography anion exchange and hydrophobic interaction chr...

journal_title：Applied and environmental microbiology

authors： Bronnenmeier K,Kern A,Liebl W,Staudenbauer WL

更新日期：1995-04-01 00:00:00

abstract：:Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...

journal_title：Applied and environmental microbiology

authors： Jian H,Xiao X,Wang F

更新日期：2013-11-01 00:00:00

abstract：:Peanut kernels (Tainan 9, a Spanish cultivar) inoculated with Aspergillus parasiticus, A. flavus, A. niger, or A. ochraceus as well as noninoculated kernels were incubated in a humidified environment (relative humidity, 100%) at 25(deg)C for 7 weeks. Internal fungal populations and changes in moisture and sucrose cont...

journal_title：Applied and environmental microbiology

authors： Chiou RY

更新日期：1997-03-01 00:00:00

abstract：:Sixty-three strains of lactic streptococci isolated from commercial lactic streptococcal starter cultures were examined for lysogeny by treatment with ultraviolet light or mitomycin C. After treatment with the inducing agent, all strains, whether or not they lysed, were examined for evidence of phage release by electr...

journal_title：Applied and environmental microbiology

authors： Huggins AR,Sandine WE

更新日期：1977-01-01 00:00:00

abstract：:Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains of Fusarium graminearum isolated from maize in Korea. One of these strains, DK21, was examined in more detail. This str...

journal_title：Applied and environmental microbiology

authors： Chu YM,Jeon JJ,Yea SJ,Kim YH,Yun SH,Lee YW,Kim KH

更新日期：2002-05-01 00:00:00

abstract：:Seven different sources of inocula that included sediments, contaminated soils, groundwater, process effluent, and sludge were used to establish enrichment cultures of denitrifying bacteria on benzene, toluene, and xylenes in the absence of molecular oxygen. All of the enrichment cultures demonstrated complete depleti...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Mang DT,Young LY

更新日期：1991-02-01 00:00:00

abstract：:Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization. ...

journal_title：Applied and environmental microbiology

authors： Francis DW,Spaulding PL,Lovett J

更新日期：1980-07-01 00:00:00

abstract：:The anoxygenic phototrophic bacterium Rhodopseudomonas palustris produces methane (CH4) from carbon dioxide (CO2) and hydrogen (H2) from protons (H+) when it expresses a variant form of molybdenum (Mo) nitrogenase that has two amino acid substitutions near its active site. We examined the influence of light energy and...

journal_title：Applied and environmental microbiology

authors： Zheng Y,Harwood CS

更新日期：2019-04-18 00:00:00

abstract：:The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

journal_title：Applied and environmental microbiology

authors： Desomer J,Dhaese P,Montagu MV

更新日期：1990-09-01 00:00:00

abstract：:Genome sequencing of the thermophilic archaeon Pyrococcus horikoshii OT3 revealed a gene which had high sequence similarity to the gene encoding the carboxypeptidase of Sulfolobus solfataricus and also to that encoding the aminoacylase from Bacillus stearothermophilus. The gene from P. horikoshii comprises an open rea...

journal_title：Applied and environmental microbiology

authors： Ishikawa K,Ishida H,Matsui I,Kawarabayasi Y,Kikuchi H

更新日期：2001-02-01 00:00:00

abstract：:A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as the substrates. Fift...

journal_title：Applied and environmental microbiology

authors： Terrón-González L,Martín-Cabello G,Ferrer M,Santero E

更新日期：2016-04-04 00:00:00

abstract：:A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...

journal_title：Applied and environmental microbiology

authors： Chung CC,Hwang SP,Chang J

更新日期：2005-12-01 00:00:00

abstract：:Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, tran...

journal_title：Applied and environmental microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-07-01 00:00:00

abstract：:The environmental transport of Cryptosporidium spp. through combined sewer overflow (CSO) and the occurrence of several emerging human-pathogenic Cryptosporidium species in developing countries remain unclear. In this study, we collected 40 CSO samples and 40 raw wastewater samples from Shanghai, China, and examined t...

journal_title：Applied and environmental microbiology

authors： Huang C,Hu Y,Wang L,Wang Y,Li N,Guo Y,Feng Y,Xiao L

更新日期：2017-08-01 00:00:00

abstract：:A high growth rate in bacterial cultures is usually achieved by optimizing growth conditions, but metabolism of the bacterium limits the maximal growth rate attainable on the carbon source used. This limitation can be circumvented by engineering the metabolism of the bacterium. Acinetobacter baylyi has become a model ...

journal_title：Applied and environmental microbiology

authors： Kannisto M,Aho T,Karp M,Santala V

更新日期：2014-11-01 00:00:00

abstract：:A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae. Two genes, abf1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside. The genes abf1 and bxl...

journal_title：Applied and environmental microbiology

authors： Margolles-Clark E,Tenkanen M,Nakari-Setälä T,Penttilä M

更新日期：1996-10-01 00:00:00

abstract：:Washed and air-oxidized proteins from Sporomusa ovata cleaved the C-O bond of methanol or methoxyaromatics and transferred the methyl to dl-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of dl-tetrahydrofolate. Methylcorrinoid-co...

journal_title：Applied and environmental microbiology

authors： Stupperich E,Konle R

更新日期：1993-09-01 00:00:00

abstract：:The cyanophycin (CGP) synthetase gene (cphANE1) of the transposon-induced argL mutant NE1 of the cyanobacterium Nostoc ellipsosporum, which exhibits a CGP-leaky phenotype during diazotrophical growth, was cloned and expressed in Escherichia coli strain TOP10. Its amino acid sequence exhibited high similarities to CphA...

journal_title：Applied and environmental microbiology

authors： Hai T,Frey KM,Steinbüchel A

更新日期：2006-12-01 00:00:00

abstract：:Rhizomucor miehei lipase (RML), as a kind of eukaryotic protein catalyst, plays an important role in the food, organic chemical, and biofuel industries. However, RML retains its catalytic activity below 50°C, which limits its industrial applications at higher temperatures. Soluble expression of this eukaryotic protein...

journal_title：Applied and environmental microbiology

authors： Li G,Fang X,Su F,Chen Y,Xu L,Yan Y

更新日期：2018-01-02 00:00:00

abstract：:A defined substrate method was developed to simultaneously enumerate total coliforms and Escherichia coli from drinking waters without the need for confirmatory or completed tests. It is a new method based on technology that uses a hydrolyzable substrate as a specific indicator-nutrient for the target microbes. No equ...

journal_title：Applied and environmental microbiology

authors： Edberg SC,Allen MJ,Smith DB

更新日期：1988-06-01 00:00:00

abstract：:The goal of this study was to determine how enteric viruses persist within shellfish tissues. Several lines of novel evidence show that phagocytic blood cells (hemocytes) of Eastern oysters (Crassostrea virginica) play an important role in the retention of virus particles. Our results demonstrated an association of vi...

journal_title：Applied and environmental microbiology

authors： Provost K,Dancho BA,Ozbay G,Anderson RS,Richards GP,Kingsley DH

更新日期：2011-12-01 00:00:00

abstract：:In a field experiment, Pseudomonas cepacia J82rif and J51rif increased sunflower emergence in the presence of the fungus Sclerotinia sclerotiorum. Pyrrolnitrin, aminopyrrolnitrin, and monochloroaminopyrrolnitrin were isolated from J82 and identified by using thin-layer chromatography, high-performance liquid chromatog...

journal_title：Applied and environmental microbiology

authors： McLoughlin TJ,Quinn JP,Bettermann A,Bookland R

更新日期：1992-05-01 00:00:00

abstract：:Size changes during starvation of 17 marine bacterial isolates at a solid-water interface and in the liquid phase were examined. Twelve rod-shaped, hydrophilic bacteria decreased in size more rapidly at the solid surface than in the liquid phase, a result parallel to that observed previously for one of the strains at ...

journal_title：Applied and environmental microbiology

authors： Humphrey B,Kjelleberg S,Marshall KC

更新日期：1983-01-01 00:00:00

abstract：:Large surface-to-volume ratios provide optimal nutrient uptake conditions for small microorganisms in oligotrophic habitats. The surface area can be increased with appendages. Here, we describe chains of interconnecting vesicles protruding from cells of strain Hel3_A1_48, affiliating with Formosa spp. within the Flavo...

journal_title：Applied and environmental microbiology

authors： Fischer T,Schorb M,Reintjes G,Kolovou A,Santarella-Mellwig R,Markert S,Rhiel E,Littmann S,Becher D,Schweder T,Harder J

更新日期：2019-09-17 00:00:00

abstract：:The growth of Vibrio parahaemolyticus in a liquid medium was compared with that of human fecal flora and estuarine flora. No marked differences were noted between growth at 25 and 37 degrees C for V. parahaemolyticus. However, the marine organisms were strongly inhibited when incubated at 37 degrees C. Incubation for ...

journal_title：Applied and environmental microbiology

authors： Dupray E,Cormier M

更新日期：1983-11-01 00:00:00