Abstract:
:The goal of this study was to determine how enteric viruses persist within shellfish tissues. Several lines of novel evidence show that phagocytic blood cells (hemocytes) of Eastern oysters (Crassostrea virginica) play an important role in the retention of virus particles. Our results demonstrated an association of virus contamination with hemocytes but not with hemolymph. Live oysters contaminated overnight with hepatitis A virus (HAV) and murine norovirus (MNV) had 56% and 80% of extractable virus associated with hemocytes, respectively. Transfer of HAV-contaminated hemocytes to naïve (virus-free) oysters resulted in naïve oyster meat testing HAV positive for up to 3 weeks. Acid tolerance of HAV, MNV, poliovirus (PV), and feline calicivirus (FCV) correlated with the ability of each virus to persist within oysters. Using reverse transcription-PCR (RT-PCR) to evaluate persistence of these viruses in oysters, we showed that HAV persisted the longest (>21 days) and was most acid resistant, MNV and PV were less tolerant of acidic pH, persisting for up to 12 days and 1 day, respectively, and FCV did not persist (<1 day) within oysters and was not acid tolerant. This suggests that the ability of a virus to tolerate the acidic conditions typical of phagolysosomal vesicles within hemocytes plays a role in determining virus persistence in shellfish. Evaluating oyster and hemocyte homogenates and live contaminated oysters as a prelude to developing improved viral RNA extraction methods, we found that viruses were extracted more expediently from hemocytes than from whole shellfish tissues and gave similar RT-PCR detection sensitivities.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Provost K,Dancho BA,Ozbay G,Anderson RS,Richards GP,Kingsley DHdoi
10.1128/AEM.06887-11subject
Has Abstractpub_date
2011-12-01 00:00:00pages
8360-9issue
23eissn
0099-2240issn
1098-5336pii
AEM.06887-11journal_volume
77pub_type
杂志文章abstract::Nonribosomal peptides (NRPs) are a class of secondary metabolites usually produced by microorganisms. They are of paramount importance in different applications, including biocontrol and pharmacy. Brevibacillus spp. are a rich source of NRPs yet have received little attention. In this study, we characterize four novel...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01981-20
更新日期:2020-11-24 00:00:00
abstract::Microbiological, genomic and transcriptomic analyses were used to examine three species from the bacterial genus Caldicellulosiruptor with respect to their capacity to convert the carbohydrate content of lignocellulosic biomass at 70°C to simple sugars, acetate, lactate, CO2, and H2. Caldicellulosiruptor bescii, C. kr...
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pub_type: 杂志文章
doi:10.1128/AEM.01622-15
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01198-16
更新日期:2016-08-15 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.2.365-370.1987
更新日期:1987-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02728-12
更新日期:2013-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.2.796-799.1997
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.2.297-305.1978
更新日期:1978-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.1.390-398.2003
更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.01415-06
更新日期:2007-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.05815-11
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.6.1374-1377.1981
更新日期:1981-06-01 00:00:00
abstract::Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.7.2302-2307.1992
更新日期:1992-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.34.2.185-188.1977
更新日期:1977-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.3.889-891.1991
更新日期:1991-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00102-20
更新日期:2020-04-17 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.6.2011-2015.1992
更新日期:1992-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.2.371-374.1981
更新日期:1981-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.7.2262-2264.1990
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.9.3486-3489.1995
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abstract::Nervous necrosis virus (NNV) is a member of the Betanodavirus genus that causes fatal diseases in over 40 species of fish worldwide. Mortality among NNV-infected fish larvae is almost 100%. In order to elucidate the mechanisms responsible for the susceptibility of fish larvae to NNV, we exposed zebrafish larvae to NNV...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-05-15 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.1.58-64.2005
更新日期:2005-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.4.864-871.1980
更新日期:1980-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.2.242-243.1977
更新日期:1977-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.1.544-550.2006
更新日期:2006-01-01 00:00:00
abstract::Volume 61, no. 4, p. 1211, column 2, line 40: this sentence should read as follows. "The viable-culture methods for Legionella sp. detection, however, often fail, and the Centers for Disease Control and Prevention has turned, when necessary, to PCR for epidemiological investigations of Legionnaires' disease and Pontia...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
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abstract:UNLABELLED:Microbes can produce molecular hydrogen (H2) via fermentation, dinitrogen fixation, or direct photolysis, yet the H2 dynamics in cyanobacterial communities has only been explored in a few natural systems and mostly in the laboratory. In this study, we investigated the diel in situ H2 dynamics in a hot spring...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2016-06-30 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00215-14
更新日期:2014-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.11.5030-5034.2000
更新日期:2000-11-01 00:00:00
abstract::The utilization by Alcaligenes faecalis of electrodes as the electron donor for denitrification was investigated in this study. The denitrification rate of A. faecalis with a poised potential was greatly enhanced compared with that of the controls without poised potentials. For nitrate reduction, although A. faecalis ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00683-15
更新日期:2015-08-15 00:00:00