Mortality Caused by Bath Exposure of Zebrafish (Danio rerio) Larvae to Nervous Necrosis Virus Is Limited to the Fourth Day Postfertilization.

abstract：:The analysis of the virulence determinants of phytopathogenic Rhodococcus fascians has been hampered by the lack of a system for introducing exogenous DNA. We investigated the possibility of genetic transformation of R. fascians by high-voltage electroporation of intact bacterial cells in the presence of plasmid DNA. ...

journal_title：Applied and environmental microbiology

authors： Desomer J,Dhaese P,Montagu MV

更新日期：1990-09-01 00:00:00

abstract：:Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom Thalassiosira pseudonana, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. ...

journal_title：Applied and environmental microbiology

authors： Hunter JE,Brandsma J,Dymond MK,Koster G,Moore CM,Postle AD,Mills RA,Attard GS

更新日期：2018-03-01 00:00:00

abstract：:Two constructs derived from the alpha-amylase gene (amyA) of Lactobacillus amylovorus were expressed in Lactobacillus plantarum, and their expression products were purified, characterized, and compared. These products correspond to the complete (AmyA) and truncated (AmyADelta) forms of alpha-amylase; AmyADelta lacks t...

journal_title：Applied and environmental microbiology

authors： Rodriguez Sanoja R,Morlon-Guyot J,Jore J,Pintado J,Juge N,Guyot JP

更新日期：2000-08-01 00:00:00

abstract：:Excessive NH(3) production in the rumen is a major nutritional inefficiency in ruminant animals. Experiments were undertaken to compare the rates of NH(3) production from different substrates in ruminal fluid in vitro and to assess the role of asaccharolytic bacteria in NH(3) production. Ruminal fluid was taken from f...

journal_title：Applied and environmental microbiology

authors： Eschenlauer SC,McKain N,Walker ND,McEwan NR,Newbold CJ,Wallace RJ

更新日期：2002-10-01 00:00:00

abstract：:Pectin is a complex uronic acid-containing polysaccharide typically found in plant cell walls, though forms of pectin are also found in marine diatoms and seagrasses. Genetic loci that target pectin have recently been identified in two phyla of marine bacteria. These loci appear to encode a pectin saccharification pat...

journal_title：Applied and environmental microbiology

authors： Hobbs JK,Hettle AG,Vickers C,Boraston AB

更新日期：2018-12-13 00:00:00

abstract：:Campylobacter jejuni was isolated from raw milk by a method that can routinely detect less than or equal to 1 organism per ml. This procedure was used in a survey of 195 separate farms and showed a 1.5% incidence of C. jejuni in milk from bulk tanks. ...

journal_title：Applied and environmental microbiology

authors： Lovett J,Francis DW,Hunt JM

更新日期：1983-08-01 00:00:00

abstract：:We examined the effect of different sediment types on the staining effectiveness of the fluorochrome DAPI (4'-6-diamidino-2-phenylindole dihydrochloride) over a wide range of concentrations and on the masking effect of sediment particles on DAPI-stained bacteria. Sediment type greatly affects the staining efficiency o...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Kalff J,Rasmussen JB

更新日期：1989-05-01 00:00:00

abstract：:Seventeen Bradyrhizobium sp. strains and one Azorhizobium strain were compared on the basis of five genetic and phenetic features: (i) partial sequence analyses of the 16S rRNA gene (rDNA), (ii) randomly amplified DNA polymorphisms (RAPD) using three oligonucleotide primers, (iii) total cellular protein profiles, (iv)...

journal_title：Applied and environmental microbiology

authors： Van Rossum D,Schuurmans FP,Gillis M,Muyotcha A,Van Verseveld HW,Stouthamer AH,Boogerd FC

更新日期：1995-04-01 00:00:00

abstract：:To ascertain the health risks that may be posed by the land application of sewage sludges, a scheme was devised to determine the types and numbers of pathogenic and potentially pathogenic bacteria present in sludges. A processing treatment was adapted to sludge to give a homogenate which yielded the greatest numbers o...

journal_title：Applied and environmental microbiology

authors： Dudley DJ,Guentzel MN,Ibarra MJ,Moore BE,Sagik BP

更新日期：1980-01-01 00:00:00

abstract：:Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...

journal_title：Applied and environmental microbiology

authors： Hood MA,Ness GE,Rodrick GE

更新日期：1981-02-01 00:00:00

abstract：:Although Pelobacter species are closely related to Geobacter species, recent studies suggested that Pelobacter carbinolicus may reduce Fe(III) via a different mechanism because it lacks the outer-surface c-type cytochromes that are required for Fe(III) reduction by Geobacter sulfurreducens. Investigation into the mech...

journal_title：Applied and environmental microbiology

authors： Haveman SA,DiDonato RJ Jr,Villanueva L,Shelobolina ES,Postier BL,Xu B,Liu A,Lovley DR

更新日期：2008-07-01 00:00:00

abstract：:Anaerobic acetate degradation at 70 degrees C and at 55 degrees C (as a reference) was studied by running laboratory upflow anaerobic sludge blanket (UASB) reactors inoculated with mesophilic granular sludge. In UASB reactors fed with acetate-containing media (3 g of chemical oxygen demand [COD] per liter, correspondi...

journal_title：Applied and environmental microbiology

authors： Rintala J,Lepistö S,Ahring B

更新日期：1993-06-01 00:00:00

abstract：:The probability of recovering pathogenic Escherichia coli from food by the Bacteriological Analytical Manual method was determined by the effects of several factors: the number of strains per food, the ability of pathogenic strains to survive enrichment, and the frequency of plasmid loss during enrichment. Biochemical...

journal_title：Applied and environmental microbiology

authors： Hill WE,Ferreira JL,Payne WL,Jones VM

更新日期：1985-06-01 00:00:00

abstract：:A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilizatio...

journal_title：Applied and environmental microbiology

authors： Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

更新日期：1993-06-01 00:00:00

abstract：:Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only c...

journal_title：Applied and environmental microbiology

authors： Morales SE,Holben WE

更新日期：2009-05-01 00:00:00

abstract：:Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

journal_title：Applied and environmental microbiology

authors： Fierer N,Jackson JA,Vilgalys R,Jackson RB

更新日期：2005-07-01 00:00:00

abstract：:Juvenile oyster disease (JOD) causes significant annual mortalities of hatchery-produced Eastern oysters, Crassostrea virginica, cultured in the Northeast. We have reported that a novel species of the alpha-proteobacteria Roseobacter group (designated CVSP) was numerically dominant in JOD-affected animals sampled duri...

journal_title：Applied and environmental microbiology

authors： Boettcher KJ,Barber BJ,Singer JT

更新日期：2000-09-01 00:00:00

abstract：:Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to...

journal_title：Applied and environmental microbiology

authors： Upton ME,Fogarty WM

更新日期：1977-01-01 00:00:00

abstract：:The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...

journal_title：Applied and environmental microbiology

authors： He R,Zeng J,Zhao D,Huang R,Yu Z,Wu QL

更新日期：2020-07-02 00:00:00

abstract：:Understanding of functional diversity of microbial populations has lagged description of their molecular diversity. Differences in substrate specificity, kinetics, products, and regulation can dramatically influence phenotypic variation among closely related strains, features that are missed when the strains studied a...

journal_title：Applied and environmental microbiology

authors： Rhodes AN,Fulthorpe RR,Tiedje JM

更新日期：2013-11-01 00:00:00

abstract：UNLABELLED:In the plant-beneficial bacterium Pseudomonas putida KT2440, three genes have been identified that encode posttranscriptional regulators of the CsrA/RsmA family. Their regulatory roles in the motile and sessile lifestyles of P. putida have been investigated by generating single-, double-, and triple-null mut...

journal_title：Applied and environmental microbiology

authors： Huertas-Rosales Ó,Ramos-González MI,Espinosa-Urgel M

更新日期：2016-08-30 00:00:00

abstract：:To understand how microbial communities and functional genes respond to arsenic contamination in the rhizosphere of Pteris vittata, five soil samples with different arsenic contamination levels were collected from the rhizosphere of P. vittata and nonrhizosphere areas and investigated by Biolog, geochemical, and funct...

journal_title：Applied and environmental microbiology

authors： Xiong J,Wu L,Tu S,Van Nostrand JD,He Z,Zhou J,Wang G

更新日期：2010-11-01 00:00:00

abstract：:Perchlorate is a known health hazard for humans, fish, and other species. Therefore, it is important to assess the response of an ecosystem exposed to perchlorate contamination. The data reported here show that a liquid chromatography-mass spectrometry-based proteomics approach for the detection of perchlorate-reducin...

journal_title：Applied and environmental microbiology

authors： Bansal R,Crawford RL,Paszczynski AJ

更新日期：2011-02-01 00:00:00

abstract：:Two alpha-amylase genes from the thermophilic alkaliphile Anaerobranca gottschalkii were cloned, and the corresponding enzymes, AmyA and AmyB, were investigated after purification of the recombinant proteins. Based on their amino acid sequences, AmyA is proposed to be a lipoprotein with extracellular localization and ...

journal_title：Applied and environmental microbiology

authors： Ballschmiter M,Armbrecht M,Ivanova K,Antranikian G,Liebl W

更新日期：2005-07-01 00:00:00

abstract：:Novel strains of obligately chemolithoautotrophic, sulfur-oxidizing bacteria have been isolated from various depths of Lake Fryxell, Antarctica. Physiological, morphological, and phylogenetic analyses showed these strains to be related to mesophilic Thiobacillus species, such as T. thioparus. However, the psychrotoler...

journal_title：Applied and environmental microbiology

authors： Sattley WM,Madigan MT

更新日期：2006-08-01 00:00:00

abstract：:The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

更新日期：2004-08-01 00:00:00

abstract：:DNA and peptide nucleic acid (PNA) molecular beacons were successfully used to detect rRNA in solution. In addition, PNA molecular beacon hybridizations were found to be useful for the quantification of rRNA: hybridization signals increased in a linear fashion with the 16S rRNA concentrations used in this experiment (...

journal_title：Applied and environmental microbiology

authors： Xi C,Balberg M,Boppart SA,Raskin L

更新日期：2003-09-01 00:00:00

abstract：:"Viili," a fermented milk product, has a firm but viscous consistency. It is produced with traditional mesophilic mixed-strain starters, which have various stabilities in dairy practice. Thirteen morphologically different types of phages were found in 90 viili samples studied by electron microscopy. Ten of the phage t...

journal_title：Applied and environmental microbiology

authors： Saxelin ML,Nurmiaho-Lassila EL,Meriläinen VT,Forsén RI

更新日期：1986-10-01 00:00:00

abstract：:Diet is a major factor in maintaining a healthy human gastrointestinal tract, and this has triggered the development of functional foods containing a probiotic and/or prebiotic component intended to improve the host's health via modulation of the intestinal microbiota. In this study, a long-term placebo-controlled cro...

journal_title：Applied and environmental microbiology

authors： Vanhoutte T,De Preter V,De Brandt E,Verbeke K,Swings J,Huys G

更新日期：2006-09-01 00:00:00

abstract：:Copper resistance mechanisms provide an important adaptive advantage to plant pathogenic bacteria under exposure to copper treatments. Copper resistance determinants have been described in Pseudomonas syringae pv. syringae (Pss) strains isolated from mango intimately associated with 62 kb plasmids belonging to the pPT...

journal_title：Applied and environmental microbiology

authors： Aprile F,Heredia-Ponce Z,Cazorla FM,de Vicente A,Gutiérrez-Barranquero JA

更新日期：2020-12-23 00:00:00