Quantitative Determination of the Spatial Distribution of Nitrosomonas europaea and Nitrobacter agilis Cells Immobilized in kappa-Carrageenan Gel Beads by a Specific Fluorescent-Antibody Labelling Technique.

Abstract:

:A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilization and allowed to grow out to colonies. The gel beads were sliced in thin cross sections after fixation and embedding. A two-step labelling method resulted in green fluorescent colonies of either N. europaea or N. agilis in the respective cross sections. The positions and surface areas of the colonies of each species were determined, and from that a biomass volume distribution for N. europaea and N. agilis in kappa-carrageenan gel beads was estimated. This technique will be useful for the validation of biofilm models, which predict such biomass distributions.

journal_name

Appl Environ Microbiol

authors

Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

doi

10.1128/AEM.59.6.1951-1954.1993

keywords:

subject

Has Abstract

pub_date

1993-06-01 00:00:00

pages

1951-4

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

59

pub_type

杂志文章
  • Thermocrinis ruber gen. nov., sp. nov., A pink-filament-forming hyperthermophilic bacterium isolated from yellowstone national park

    abstract::A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occurring in the upper outflow channel (temperature, 82 to 88 degreesC) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89 degreesC in the neutral to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.3576-3583.1998

    authors: Huber R,Eder W,Heldwein S,Wanner G,Huber H,Rachel R,Stetter KO

    更新日期:1998-10-01 00:00:00

  • Suppressive effect on activation of macrophages by Lactobacillus casei strain Shirota genes determining the synthesis of cell wall-associated polysaccharides.

    abstract::Although many Lactobacillus strains used as probiotics are believed to modulate host immune responses, the molecular natures of the components of such probiotic microorganisms directly involved in immune modulation process are largely unknown. We aimed to assess the function of polysaccharide moiety of the cell wall o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00412-08

    authors: Yasuda E,Serata M,Sako T

    更新日期:2008-08-01 00:00:00

  • Genetic and symbiotic diversity of nitrogen-fixing bacteria isolated from agricultural soils in the western Amazon by using cowpea as the trap plant.

    abstract::Cowpea is a legume of great agronomic importance that establishes symbiotic relationships with nitrogen-fixing bacteria. However, little is known about the genetic and symbiotic diversity of these bacteria in distinct ecosystems. Our study evaluated the genetic diversity and symbiotic efficiencies of 119 bacterial str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01303-12

    authors: Azarias Guimarães A,Duque Jaramillo PM,Simão Abrahão Nóbrega R,Florentino LA,Barroso Silva K,de Souza Moreira FM

    更新日期:2012-09-01 00:00:00

  • Characterization of cadmium uptake in Lactobacillus plantarum and isolation of cadmium and manganese uptake mutants.

    abstract::Two different Cd(2+) uptake systems were identified in Lactobacillus plantarum. One is a high-affinity, high-velocity Mn(2+) uptake system which also takes up Cd(2+) and is induced by Mn(2+) starvation. The calculated K(m) and V(max) are 0.26 microM and 3.6 micromol g of dry cell(-1) min(-1), respectively. Unlike Mn(2...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.11.4741-4745.1999

    authors: Hao Z,Reiske HR,Wilson DB

    更新日期:1999-11-01 00:00:00

  • Genetic characterization of plasmid-associated benzalkonium chloride resistance determinants in a Listeria monocytogenes strain from the 1998-1999 outbreak.

    abstract::Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02056-10

    authors: Elhanafi D,Dutta V,Kathariou S

    更新日期:2010-12-01 00:00:00

  • Production and Characterization of Trametes versicolor Mutants Unable To Bleach Hardwood Kraft Pulp.

    abstract::Protoplasts of the monokaryotic strain 52J of Trametes versicolor were treated with UV light and screened for the inability to produce a colored precipitate on guaiacol-containing agar plates. Mutants unable to oxidize guaiacol had absent or very low secretion of laccase and manganese peroxidase (MnP) proteins. All is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.10.3687-3694.1995

    authors: Addleman K,Dumonceaux T,Paice MG,Bourbonnais R,Archibald FS

    更新日期:1995-10-01 00:00:00

  • Habitat-specific diversity of Borrelia burgdorferi sensu lato in Europe, exemplified by data from Latvia.

    abstract::The distribution of Borrelia burgdorferi sensu lato genospecies in questing Ixodes ricinus ticks from ecologically distinct habitats in Latvia was analyzed. A significant variation in the frequency of the genospecies across sites was observed, pointing to the importance of the host community in the ecology of Lyme bor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.5.3008-3010.2003

    authors: Etti S,Hails R,Schäfer SM,De Michelis S,Sewell HS,Bormane A,Donaghy M,Kurtenbach K

    更新日期:2003-05-01 00:00:00

  • Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida F1 toluene dioxygenase genes.

    abstract::Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroet...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.12.3162-3166.1989

    authors: Zylstra GJ,Wackett LP,Gibson DT

    更新日期:1989-12-01 00:00:00

  • Metabolic engineering of Escherichia coli for efficient conversion of glycerol to ethanol.

    abstract::Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00670-09

    authors: Trinh CT,Srienc F

    更新日期:2009-11-01 00:00:00

  • Development of monoclonal antibodies that identify Vibrio species commonly isolated from infections of humans, fish, and shellfish.

    abstract::Monoclonal antibodies (MAbs) against Vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. The pathogens included Vibrio alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus, and V. vul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.11.3694-3700.1992

    authors: Chen D,Hanna PJ,Altmann K,Smith A,Moon P,Hammond LS

    更新日期:1992-11-01 00:00:00

  • Competition for light between toxic and nontoxic strains of the harmful cyanobacterium Microcystis.

    abstract::The cyanobacterium Microcystis can produce microcystins, a family of toxins that are of major concern in water management. In several lakes, the average microcystin content per cell gradually declines from high levels at the onset of Microcystis blooms to low levels at the height of the bloom. Such seasonal dynamics m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02892-06

    authors: Kardinaal WE,Tonk L,Janse I,Hol S,Slot P,Huisman J,Visser PM

    更新日期:2007-05-01 00:00:00

  • Subsurface microbial methanotrophic mats in the Black Sea.

    abstract::A nodule-shaped microbial mat was found subsurface in sediments of a gas seep in the anoxic Black Sea. This mat was dominated by ANME-1 archaea and consumed methane and sulfate simultaneously. We propose that such subsurface mats represent the initial stage of previously investigated microbial reefs. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.10.6375-6378.2005

    authors: Treude T,Knittel K,Blumenberg M,Seifert R,Boetius A

    更新日期:2005-10-01 00:00:00

  • Effects of GPD1 overexpression in Saccharomyces cerevisiae commercial wine yeast strains lacking ALD6 genes.

    abstract::The utilization of Saccharomyces cerevisiae strains overproducing glycerol and with a reduced ethanol yield is a potentially valuable strategy for producing wine with decreased ethanol content. However, glycerol overproduction is accompanied by acetate accumulation. In this study, we evaluated the effects of the overe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02975-05

    authors: Cambon B,Monteil V,Remize F,Camarasa C,Dequin S

    更新日期:2006-07-01 00:00:00

  • Evaluation of indoxyl-beta-D-glucuronide as a chromogen in media specific for Escherichia coli.

    abstract::Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.8.2758-2759.1993

    authors: Haines JR,Covert TC,Rankin CC

    更新日期:1993-08-01 00:00:00

  • Microbial communities associated with geological horizons in coastal subseafloor sediments from the sea of okhotsk.

    abstract::Microbial communities from a subseafloor sediment core from the southwestern Sea of Okhotsk were evaluated by performing both cultivation-dependent and cultivation-independent (molecular) analyses. The core, which extended 58.1 m below the seafloor, was composed of pelagic clays with several volcanic ash layers contai...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.12.7224-7235.2003

    authors: Inagaki F,Suzuki M,Takai K,Oida H,Sakamoto T,Aoki K,Nealson KH,Horikoshi K

    更新日期:2003-12-01 00:00:00

  • Isolation of Rickettsia parkeri and identification of a novel spotted fever group Rickettsia sp. from Gulf Coast ticks (Amblyomma maculatum) in the United States.

    abstract::Until recently, Amblyomma maculatum (the Gulf Coast tick) had garnered little attention compared to other species of human-biting ticks in the United States. A. maculatum is now recognized as the principal vector of Rickettsia parkeri, a pathogenic spotted fever group rickettsia (SFGR) that causes an eschar-associated...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02737-09

    authors: Paddock CD,Fournier PE,Sumner JW,Goddard J,Elshenawy Y,Metcalfe MG,Loftis AD,Varela-Stokes A

    更新日期:2010-05-01 00:00:00

  • Increased crystalline cellulose activity via combinations of amino acid changes in the family 9 catalytic domain and family 3c cellulose binding module of Thermobifida fusca Cel9A.

    abstract::Amino acid modifications of the Thermobifida fusca Cel9A-68 catalytic domain or carbohydrate binding module 3c (CBM3c) were combined to create enzymes with changed amino acids in both domains. Bacterial crystalline cellulose (BC) and swollen cellulose (SWC) assays of the expressed and purified enzymes showed that thre...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02735-09

    authors: Li Y,Irwin DC,Wilson DB

    更新日期:2010-04-01 00:00:00

  • Monitoring microbial populations and antibiotic resistance gene enrichment associated with Arctic waste stabilization ponds.

    abstract::Wastewater management in the Canadian Arctic is challenging due to climate extremes, small population sizes, and lack of conventional infrastructure for wastewater treatment. Although many Northern communities use waste stabilization ponds (WSPs) as their primary form of wastewater treatment, few studies have explored...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02914-20

    authors: Gromala M,Neufeld JD,McConkey BJ

    更新日期:2021-01-15 00:00:00

  • Evaluation of coliphage detection as a rapid indicator of water quality.

    abstract::A rapid coliphage analysis technique for enumerating coliphages in natural waters has been evaluated by water quality laboratories located throughout the United States. Correlations were established between coliphages and coliforms in natural water systems. These correlations were highly significant. This relationship...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.430-434.1982

    authors: Wentsel RS,O'Neill PE,Kitchens JF

    更新日期:1982-02-01 00:00:00

  • Utilization of ammonia nitrogen by intestinal bacteria isolated from pigs.

    abstract::In a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 Bacteroidaceae strains, 3 of Selenomonas strains, 1 of 7 curved rods, 3 of 7 Spirochaetaceae strains, 8 of 20 Eubacterium strains, 8 of 13 Peptococcaceae strains, 3 of 4 Clostridium strains, 19 of 20 Enterobacteriaceae strains...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.1.30-35.1980

    authors: Takahashi M,Benno Y,Mitsuoka T

    更新日期:1980-01-01 00:00:00

  • Produce isolates of the Escherichia coli Ont:H52 serotype that carry both Shiga toxin 1 and stable toxin genes.

    abstract::Produce isolates of the Escherichia coli Ont:H52 serotype carried Shiga toxin 1 and stable toxin genes but only expressed Stx1. These strains had pulsed-field gel electrophoresis profiles that were 90% homologous to clinical Ont:H52 strains that had identical phenotypes and genotypes. All Ont:H52 strains had identical...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.72.4.3062-3065.2006

    authors: Monday SR,Keys C,Hanson P,Shen Y,Whittam TS,Feng P

    更新日期:2006-04-01 00:00:00

  • Use of murine norovirus as a surrogate to evaluate resistance of human norovirus to disinfectants.

    abstract::Murine norovirus (MNV) was used as a surrogate to study resistance of human norovirus to disinfectants used in hospitals. MNV was sensitive to alcohol, alcohol hand rubs, bleach, and povidone iodine-based disinfectant. Real-time reverse transcription-PCR results indicated that the presence of viral RNA did not correla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02148-07

    authors: Belliot G,Lavaux A,Souihel D,Agnello D,Pothier P

    更新日期:2008-05-01 00:00:00

  • Evidence for coexistence of two distinct functional groups of sulfate-reducing bacteria in salt marsh sediment.

    abstract::Oxidation of acetate in salt marsh sediment was inhibited by the addition of fluoroacetate, and also by the addition of molybdate, an inhibitor of sulfate-reducing bacteria. Molybdate had no effect upon the metabolism of acetate in a freshwater sediment in the absence of sulfate. The inhibitory effect of molybdate on ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.42.6.985-992.1981

    authors: Banat IM,Lindström EB,Nedwell DB,Balba MT

    更新日期:1981-12-01 00:00:00

  • Distribution and Genetic Diversity of Bacteriocin Gene Clusters in Rumen Microbial Genomes.

    abstract::Some species of ruminal bacteria are known to produce antimicrobial peptides, but the screening procedures have mostly been based on in vitro assays using standardized methods. Recent sequencing efforts have made available the genome sequences of hundreds of ruminal microorganisms. In this work, we performed genome mi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01223-15

    authors: Azevedo AC,Bento CB,Ruiz JC,Queiroz MV,Mantovani HC

    更新日期:2015-10-01 00:00:00

  • Stereospecific production of the herbicide phosphinothricin (glufosinate) by transamination: isolation and characterization of a phosphinothricin-specific transaminase from Escherichia coli.

    abstract::An aminotransferase capable of transaminating 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid to L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG), was purified to apparent homogeneity from Escherichia coli K-12. The enzyme catalyzes the trans...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.1.1-6.1990

    authors: Schulz A,Taggeselle P,Tripier D,Bartsch K

    更新日期:1990-01-01 00:00:00

  • Two-step freezing procedure for cryopreservation of rumen ciliates, an effective tool for creation of a frozen rumen protozoa bank.

    abstract::The present study aimed at the long-term storage of rumen protozoa as living cells in liquid nitrogen. The two-step or interrupted slow freezing procedure was used to cryopreserve six of the dominant species of rumen ciliates isolated from monofaunated animals, Dasytricha ruminantium, Entodinium caudatum, Epidinium ec...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.3826-3832.2003

    authors: Nsabimana E,Kisidayová S,Macheboeuf D,Newbold CJ,Jouany JP

    更新日期:2003-07-01 00:00:00

  • Discovery of Two Native Baeyer-Villiger Monooxygenases for Asymmetric Synthesis of Bulky Chiral Sulfoxides.

    abstract::Two Baeyer-Villiger monooxygenases (BVMOs), designated BoBVMO and AmBVMO, were discovered from Bradyrhizobium oligotrophicum and Aeromicrobium marinum, respectively. Both monooxygenases displayed novel features for catalyzing the asymmetric sulfoxidation of bulky and pharmaceutically relevant thioethers. Evolutionary ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00638-18

    authors: Zhang Y,Liu F,Xu N,Wu YQ,Zheng YC,Zhao Q,Lin G,Yu HL,Xu JH

    更新日期:2018-07-02 00:00:00

  • Characteristics and Adaptability of Some New Isolates of Clostridium thermocellum.

    abstract::Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.3.475-477.1985

    authors: Bender J,Vatcharapijarn Y,Jeffries TW

    更新日期:1985-03-01 00:00:00

  • Inhibitor Evaluation with Immobilized Nitrobacter agilis Cells.

    abstract::Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.6.1231-1235.1986

    authors: Tsai YL,Schlasner SM,Tuovinen OH

    更新日期:1986-12-01 00:00:00

  • Tn5 Insertion Mutants of Pseudomonas fluorescens Defective in Adhesion to Soil and Seeds.

    abstract::Tn5 insertion mutants of a soil isolate, Pseudomonas fluorescens Pf0-1, were selected for decreased ability to adhere to quartz sand in a column assay. Three adhesion-deficient mutants that differed in the location of the Tn5 insertion in the chromosome were isolated and compared with the wild-type strain. One mutant,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.7.2637-2642.1994

    authors: Deflaun MF,Marshall BM,Kulle EP,Levy SB

    更新日期:1994-07-01 00:00:00