Abstract:
:Maximum yields of amylase were produced by the thermophilic actinomycete Thermomonospora viridis in a modified Simpson and McCoy medium containing 1.5% corn starch and 0.5% mycological peptone with an initial pH 7.0. Best yields of amylase were obtained after incubation for 48 h, when the pH of the medium had risen to 8.2. Amylase was purified 313-fold by precipitation with n-propyl alcohol, dialysis against tap water, adsorption on Ca(3)(PO(4))(2), and fractionation on Sephadex G-100. Protease was produced in nutrient broth containing 0.5% starch and 1.0% corn steep liquor and at an initial pH 7.0. Maximum yields of protease were produced after 42 h. The protease was purified 54-fold by precipitation with n-propyl alcohol, dialysis against tap water, adsorption on Ca(3)(PO(4))(2), and fractionation on Sephadex G-200.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Upton ME,Fogarty WMdoi
10.1128/AEM.33.1.59-64.1977keywords:
subject
Has Abstractpub_date
1977-01-01 00:00:00pages
59-64issue
1eissn
0099-2240issn
1098-5336journal_volume
33pub_type
杂志文章abstract::Anaerobic incubation of membrane filter cultures significantly enhanced detection of fecal coliforms in surface-water samples from recreational beaches. In contrast to standard aerobic incubation, anaerobic incubation suppressed overgrowth of masking, noncoliform bacteria but did not increase the frequency of fecal co...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.2.324-326.1984
更新日期:1984-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.2.827-833.2001
更新日期:2001-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.9.4085-4093.1999
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.12.5234-5241.1999
更新日期:1999-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.6.3672-3675.2003
更新日期:2003-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:2005-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.11.2737-2741.1988
更新日期:1988-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.4.1413-1419.1999
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.2.763-767.1998
更新日期:1998-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.7.2400-2407.1994
更新日期:1994-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章,随机对照试验
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更新日期:2014-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.1775-1784.2005
更新日期:2005-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.6.1761-1766.1983
更新日期:1983-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/aem.68.7.3614-3621.2002
更新日期:2002-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.46.2.480-483.1983
更新日期:1983-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.1.69-73.1988
更新日期:1988-01-01 00:00:00
abstract::The beta-agarase C gene (agaC) of a marine bacterium, Vibrio sp. strain PO-303, consisted of 1,437 bp encoding 478 amino acid residues. beta-Agarase C was identified as the first beta-agarase that cannot hydrolyze neoagarooctaose and smaller neoagarooligosaccharides and was assigned to a novel glycoside hydrolase fami...
journal_title:Applied and environmental microbiology
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更新日期:2006-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2019-10-16 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1995-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.8.4997-5000.2003
更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-03-01 00:00:00