Cloning of the novel gene encoding beta-agarase C from a marine bacterium, Vibrio sp. strain PO-303, and characterization of the gene product.

abstract：:Based on elementary mode analysis, an Escherichia coli strain was designed for efficient conversion of glycerol to ethanol. By using nine gene knockout mutations, the functional space of the central metabolism of E. coli was reduced from over 15,000 possible pathways to a total of 28 glycerol-utilizing pathways that s...

journal_title：Applied and environmental microbiology

authors： Trinh CT,Srienc F

更新日期：2009-11-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...

journal_title：Applied and environmental microbiology

authors： Ghafoor A,Hay ID,Rehm BH

更新日期：2011-08-01 00:00:00

abstract：:Primary cell cultures, a continuous cell line, and a diploid cell line were grown on an artificial capillary system. The cells were subsequently infected with Venezuelan equine encephalitis virus, and viral replication was studied. Extracellular fluids harvested from this system contained high titers of virus and were...

journal_title：Applied and environmental microbiology

authors： Johnson AD,Eddy GA,Gangemi JD,Ramsburg HH,Metzger JF

更新日期：1978-02-01 00:00:00

abstract：:Environmental studies of the human-pathogenic bacterium Campylobacter jejuni have focused on linking distributions with potential sources. However, in aquatic ecosystems, the abundance of C. jejuni may also be regulated by predation. We examine the potential for grazing by the freshwater planktonic crustacean Daphnia ...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Bremer PJ,Henkel S,Launhardt A,Burns CW

更新日期：2005-09-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) infections are a critical public health concern because they can cause severe clinical outcomes, such as hemolytic uremic syndrome, in humans. Determining the presence or absence of virulence genes is essential in assessing the potential pathogenicity of STEC strains. Curr...

journal_title：Applied and environmental microbiology

authors： Tseng M,Fratamico PM,Bagi L,Delannoy S,Fach P,Manning SD,Funk JA

更新日期：2014-10-01 00:00:00

abstract：:Vibrio tubiashii is a recently reemerging pathogen of larval bivalve mollusks, causing both toxigenic and invasive disease. Marine Vibrio spp. produce an array of extracellular products as potential pathogenicity factors. Culture supernatants of V. tubiashii have been shown to be toxic to oyster larvae and were report...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Lind EJ,Boin MA,Häse CC

更新日期：2008-07-01 00:00:00

abstract：:Microbacterium thermosphactum requires cysteine, alpha-lipoate, nicotinate, pantothenate, p-aminobenzoate, biotin, and thiamin for aerobic growth in glucose-mineral salts medium. Glucose cannot be replaced by Casamino Acids or acids of the tricarboxylic acid cycle as sole carbon and energy sources. The organism can al...

journal_title：Applied and environmental microbiology

authors： Grau FH

更新日期：1979-11-01 00:00:00

abstract：:Most associations between animals and their gut microbiota are dynamic, involving sustained transfer of food-associated microbial cells into the gut and shedding of microorganisms into the external environment with feces, but the interacting effects of host and microbial factors on the composition of the internal and ...

journal_title：Applied and environmental microbiology

authors： Wong AC,Luo Y,Jing X,Franzenburg S,Bost A,Douglas AE

更新日期：2015-09-01 00:00:00

abstract：UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

journal_title：Applied and environmental microbiology

authors： Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

更新日期：2016-07-15 00:00:00

abstract：:Research on the microbial communities that colonize stone monuments may provide a new understanding of stone biodeterioration and microbe-induced carbonate precipitation. This work investigated the seasonal variation of microbial communities in 2016 and 2017, as well as its effects on stone monuments. We determined th...

journal_title：Applied and environmental microbiology

authors： Li Q,Zhang B,Yang X,Ge Q

更新日期：2018-03-19 00:00:00

abstract：:Several selective media were evaluated for the primary isolation and enumeration of Staphylococcus aureus from halogenated indoor swimming pool waters. Standard plate counts of the viable population and total coliform densities were also determined to ascertain their value as indicator systems. All studies were done w...

journal_title：Applied and environmental microbiology

authors： Alico RK,Dragonjac MF

更新日期：1986-04-01 00:00:00

abstract：:To survive in various environments, from host tissue to soil, opportunistic bacterial pathogens must be metabolically flexible and able to use a variety of nutrient sources. We are interested in Pseudomonas aeruginosa's catabolism of quaternary amine compounds that are prevalent in association with eukaryotes. Carniti...

journal_title：Applied and environmental microbiology

authors： Meadows JA,Wargo MJ

更新日期：2013-06-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00

abstract：:Leuconostoc mesenteroides strains that are resistant to high levels of nisin (up to 25,000 IU/ml in broth) were isolated. These nisin-resistant mutants were evaluated to determine their potential use as starter culture strains for cabbage fermentations. We found that some L. mesenteroides strains could be adapted to h...

journal_title：Applied and environmental microbiology

authors： Breidt F,Crowley KA,Fleming HP

更新日期：1993-11-01 00:00:00

abstract：:Bacillus cereus endospores germinate in response to particular nutrients. Spores are able to sense these nutrients in the environment by receptors encoded by the gerA family of operons. Analysis of the Bacillus cereus ATCC 14579 genome revealed seven gerA family homologues. Using a transposon Tn917-based insertional m...

journal_title：Applied and environmental microbiology

authors： Hornstra LM,de Vries YP,de Vos WM,Abee T,Wells-Bennik MH

更新日期：2005-02-01 00:00:00

abstract：:Curli are adhesive fimbriae of Enterobacteriaceae and are involved in surface attachment, cell aggregation, and biofilm formation. Here, we report that both inter- and intrastrain variations in curli production are widespread in enterohemorrhagic Escherichia coli O157:H7. The relative proportions of curli-producing va...

journal_title：Applied and environmental microbiology

authors： Carter MQ,Brandl MT,Louie JW,Kyle JL,Carychao DK,Cooley MB,Parker CT,Bates AH,Mandrell RE

更新日期：2011-06-01 00:00:00

abstract：:Solid media on a base of B-12 or CB medium with agarose or agarose of low melting temperature were developed for the cultivation of Microcystis species. The media with 0.4% gel showed the highest number of CFU, and increasing the gel concentration resulted in a reduction of the number of CFU. There was no difference i...

journal_title：Applied and environmental microbiology

authors： Shirai M,Matumaru K,Ohotake A,Takamura Y,Aida T,Nakano M

更新日期：1989-10-01 00:00:00

abstract：:Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence...

journal_title：Applied and environmental microbiology

authors： Tardy F,Mick V,Dordet-Frisoni E,Marenda MS,Sirand-Pugnet P,Blanchard A,Citti C

更新日期：2015-03-01 00:00:00

abstract：:We modeled mold growth on a solid culture medium at various temperatures and NaCl concentrations by using five common food spoilage molds (Penicillium roqueforti, Trichoderma harzianum, Paecilomyces variotii, Aspergillus niger, and Emericella nidulans). For the description of the growth rate (expressed as the increase...

journal_title：Applied and environmental microbiology

authors： Cuppers HG,Oomes S,Brul S

更新日期：1997-10-01 00:00:00

abstract：:Auxotrophic microorganisms are often used for genetic engineering, because their biosynthetic deficiency can be complemented by the transforming DNA and allows selection for transformants that have become prototrophic. However, when complementation is obtained by ectopic expression this may lead to unpredictable side ...

journal_title：Applied and environmental microbiology

authors： Nielsen ML,de Jongh WA,Meijer SL,Nielsen J,Mortensen UH

更新日期：2007-11-01 00:00:00

abstract：:Two PCR primer pairs, based on the virD2 and ipt genes, detected a wide variety of pathogenic Agrobacterium strains. The endonuclease domain of VirD2 protein, which cleaves transferred DNA (T-DNA) border sequences, is highly conserved; primer oligonucleotides specific for the endonuclease portion of virD2 detected all...

journal_title：Applied and environmental microbiology

authors： Haas JH,Moore LW,Ream W,Manulis S

更新日期：1995-08-01 00:00:00

abstract：:Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

更新日期：2002-02-01 00:00:00

abstract：:Sporulation of Bacillus larvae NRRL B-3650 occurred only at aeration rates lower than those used for cultivation of most Bacillus species. One possible explanation for the requirement for a low level of aeration in B. larvae is that toxic forms of oxygen such as H(2)O(2) and superoxide are involved. The superoxide dis...

journal_title：Applied and environmental microbiology

authors： Dingman DW,Stahly DP

更新日期：1984-06-01 00:00:00

abstract：:The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP wit...

journal_title：Applied and environmental microbiology

authors： Jain RK,Dreisbach JH,Spain JC

更新日期：1994-08-01 00:00:00

abstract：:Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypotheti...

journal_title：Applied and environmental microbiology

authors： Simmons K,Rempel H,Block G,Forgetta V,Vaillancourt R Jr,Malouin F,Topp E,Delaquis P,Diarra MS

更新日期：2014-03-01 00:00:00

abstract：:Rapid population growth and urban development along waterways and coastal areas have led to decreasing water quality. To examine the effects of upstream anthropogenic activities on microbiological water quality, methods for source-specific testing are required. In this study, molecular assays targeting human enterovir...

journal_title：Applied and environmental microbiology

authors： Fong TT,Griffin DW,Lipp EK

更新日期：2005-04-01 00:00:00

abstract：:Biofouling of reverse-osmosis membranes was investigated at an advanced wastewater treatment facility. Cellulose diacetate membranes operated for approximately 4,000 h became uniformly coated with a mucilaginous fouling layer. The fouling material was approximately 93% water by weight, and nearly 90% of the dehydrated...

journal_title：Applied and environmental microbiology

authors： Ridgway HF,Kelly A,Justice C,Olson BH

更新日期：1983-03-01 00:00:00

abstract：:Biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. Accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotro...

journal_title：Applied and environmental microbiology

authors： Londry KL,Des Marais DJ

更新日期：2003-05-01 00:00:00

abstract：:Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

journal_title：Applied and environmental microbiology

authors： Wang CR,Chu FS

更新日期：1991-04-01 00:00:00

abstract：:Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...

journal_title：Applied and environmental microbiology

authors： Hall MJ,Ratledge C

更新日期：1977-03-01 00:00:00