A model for the combined effects of temperature and salt concentration on growth rate of food spoilage molds.

abstract：:Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization. ...

journal_title：Applied and environmental microbiology

authors： Francis DW,Spaulding PL,Lovett J

更新日期：1980-07-01 00:00:00

abstract：:Influenza virus has been found to persist in the environment for hours to days, allowing for secondary transmission of influenza via inanimate objects known as fomites. We evaluated the efficacy of heat and moisture for the decontamination of surfaces for the purpose of preventing of the spread of influenza. Aqueous s...

journal_title：Applied and environmental microbiology

authors： McDevitt J,Rudnick S,First M,Spengler J

更新日期：2010-06-01 00:00:00

abstract：:Surfaces made of copper or its alloys have strong antimicrobial properties against a wide variety of microorganisms. However, the molecular mode of action responsible for the antimicrobial efficacy of metallic copper is not known. Here, we show that dry copper surfaces inactivate Candida albicans and Saccharomyces cer...

journal_title：Applied and environmental microbiology

authors： Quaranta D,Krans T,Espírito Santo C,Elowsky CG,Domaille DW,Chang CJ,Grass G

更新日期：2011-01-01 00:00:00

abstract：:The unusual compound beta-aminoglutaric acid (beta-glutamate) has been identified by 13C nuclear magnetic resonance spectroscopy in soluble extracts of marine methanogenic bacteria. We examined several methanogen species representing nine genera and found that beta-glutamate occurred in methanococci and two methanogen...

journal_title：Applied and environmental microbiology

authors： Robertson DE,Roberts MF,Belay N,Stetter KO,Boone DR

更新日期：1990-05-01 00:00:00

abstract：:Azospirillum spp. were shown to utilize both straw and xylan, a major component of straw, for growth with an adequate combined N supply and also under N-limiting conditions. For most strains examined, a semisolid agar medium was satisfactory, but several strains appeared to be capable of slow metabolism of the agar. S...

journal_title：Applied and environmental microbiology

authors： Halsall DM,Turner GL,Gibson AH

更新日期：1985-02-01 00:00:00

abstract：:The membrane proteins IIC and IID of the mannose phosphotransferase system (Man-PTS) together form a membrane-located complex that serves as a receptor for several different bacteriocins, including the pediocin-like class IIa bacteriocins and the class IIc bacteriocin lactococcin A. Bacterial strains sensitive to clas...

journal_title：Applied and environmental microbiology

authors： Kjos M,Nes IF,Diep DB

更新日期：2011-05-01 00:00:00

abstract：:Two novel gull-specific quantitative PCR (qPCR) assays were developed using 16S rRNA gene sequences from gull fecal clone libraries: a SYBR green assay targeting Streptococcus spp. (gull3) and a hydrolysis TaqMan assay targeting Catellicoccus marimammalium (gull4). The objectives of this study were to compare the host...

journal_title：Applied and environmental microbiology

authors： Ryu H,Griffith JF,Khan IU,Hill S,Edge TA,Toledo-Hernandez C,Gonzalez-Nieves J,Santo Domingo J

更新日期：2012-03-01 00:00:00

abstract：:Indoxyl-beta-D-glucuronide (indoxyl) was evaluated as a specific chromogen for detection of Escherichia coli by the membrane filter method. In all, 413 colonies were tested from the indoxyl-supplemented media, yielding 93.3% confirmation, as E. coli. Compared with the indoxyl medium, other media gave either much lower...

journal_title：Applied and environmental microbiology

authors： Haines JR,Covert TC,Rankin CC

更新日期：1993-08-01 00:00:00

abstract：:This is the first study to characterize the environmental conditions which contribute to the presence and proliferation of environmental mycobacteria in a major freshwater river. Over 20 different species of environmental mycobacteria were isolated, including the pathogenic M. avium and M. kansasii. Species of the rap...

journal_title：Applied and environmental microbiology

authors： Bland CS,Ireland JM,Lozano E,Alvarez ME,Primm TP

更新日期：2005-10-01 00:00:00

abstract：:The gene encoding serine alkaline protease (SapSh) of the psychrotrophic bacterium Shewanella strain Ac10 was cloned in Escherichia coli. The amino acid sequence deduced from the 2,442-bp nucleotide sequence revealed that the protein was 814 amino acids long and had an estimated molecular weight of 85,113. SapSh exhib...

journal_title：Applied and environmental microbiology

authors： Kulakova L,Galkin A,Kurihara T,Yoshimura T,Esaki N

更新日期：1999-02-01 00:00:00

abstract：:Isolates of Escherichia coli belonging to clonal group A (CGA), a recently described disseminated cause of drug-resistant urinary tract infections in humans, were present in four of seven sewage effluents collected from geographically dispersed areas of the United States. All 15 CGA isolates (1% of the 1,484 isolates ...

journal_title：Applied and environmental microbiology

authors： Boczek LA,Rice EW,Johnston B,Johnson JR

更新日期：2007-07-01 00:00:00

abstract：:Phormium yellow leaf (PYL) phytoplasma causes a lethal disease of the monocotyledon, New Zealand flax (Phormium tenax). The 16S rRNA genes of PYL phytoplasma were amplified from infected flax by PCR and cloned, and the nucleotide sequences were determined. DNA sequencing and Southern hybridization analysis of genomic ...

journal_title：Applied and environmental microbiology

authors： Liefting LW,Andersen MT,Beever RE,Gardner RC,Forster RL

更新日期：1996-09-01 00:00:00

abstract：:The variable-region genes of monoclonal antibody against Bacillus cereus spores were cloned from mouse hybridoma cells by reverse transcription-PCR. The heavy- and light-chain variable-region genes were connected by a 45-base linker DNA to allow folding of the fusion protein into a functional tertiary structure. For d...

journal_title：Applied and environmental microbiology

authors： Koo K,Foegeding PM,Swaisgood HE

更新日期：1998-07-01 00:00:00

abstract：:A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic DNA of the closely...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Valster RM,Wullings BA,Boonstra H,Smidt H,van der Kooij D

更新日期：2006-09-01 00:00:00

abstract：:The conjugal transfer of R-plasmids is known to occur at temperatures above 22 degrees C. We found that R1drd-19 is transferable below 22 degrees C, and we discuss this finding in the context of plasmid transfer in environmental waters. ...

journal_title：Applied and environmental microbiology

authors： Singleton P,Anson AE

更新日期：1981-11-01 00:00:00

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:Lipopolysaccharides (LPS) were isolated from four species of cyanobacteria (Anabaena flos-aquae UTEX 1444. A. cylindrica, Oscillatoria tenuis, and O. brevis) frequently occurring in drinking-water supplies. The cyanobacterial LPS contained glucose, xylose, mannose, and rhamnose, but differed from the LPS derived from ...

journal_title：Applied and environmental microbiology

authors： Keleti G,Sykora JL

更新日期：1982-01-01 00:00:00

abstract：:A defined substrate method was developed to simultaneously enumerate total coliforms and Escherichia coli from drinking waters without the need for confirmatory or completed tests. It is a new method based on technology that uses a hydrolyzable substrate as a specific indicator-nutrient for the target microbes. No equ...

journal_title：Applied and environmental microbiology

authors： Edberg SC,Allen MJ,Smith DB

更新日期：1988-06-01 00:00:00

abstract：:The presence and chain length of mycolic acids of bacteria of the genera Corynebacterium, Rhodococcus, Gordona, Mycobacterium, and Arthrobacter and of coryneform bacteria containing a type B peptidoglycan were related to the cell surface hydrophobicity of the bacteria, which in turn was related to adhesion of the cell...

journal_title：Applied and environmental microbiology

authors： Bendinger B,Rijnaarts HH,Altendorf K,Zehnder AJ

更新日期：1993-11-01 00:00:00

abstract：:In the presence of added sulfate, 2,4-dichlorophenol and 4-chlorophenol were transformed stoichiometrically to 4-chlorophenol and phenol, respectively, in anaerobic freshwater lake sediments between 18 and 40 degrees C. The concomitantly occurring sulfate reduction reduced the initial sulfate concentration from 25 mM ...

journal_title：Applied and environmental microbiology

authors： Kohring GW,Zhang XM,Wiegel J

更新日期：1989-10-01 00:00:00

abstract：:A norleucine-resistant mutant was derived from an isoleucine-valine auxotroph of a leucine accumulator of Serratia marcescens. The norleucine-resistant mutant could accumulate norleucine from norvaline in the medium without the addition of methionine, which antagonized norleucine. This mutant constitutively formed hom...

journal_title：Applied and environmental microbiology

authors： Kisumi M,Sugiura M,Chibata I

更新日期：1977-08-01 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00

abstract：:Clostridium thermohydrosulfuricum 39E produced a cell-bound cyclodextrin (CD)-degrading enzyme (cyclodextrinase). It was partially purified 205-fold (specific activity, 14.5 U/mg of protein) by solubilizing with Triton X-100, ammonium sulfate treatment, and DEAE-Sepharose CL-6B column chromatography. The enzyme activi...

journal_title：Applied and environmental microbiology

authors： Saha BC,Zeikus JG

更新日期：1990-09-01 00:00:00

abstract：:Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

journal_title：Applied and environmental microbiology

authors： Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

更新日期：2007-10-01 00:00:00

abstract：:The gray mold fungus Botrytis cinerea is a major threat to fruit and vegetable production. Strawberry fields usually receive several fungicide treatments against Botrytis per season. Gray mold isolates from several German strawberry-growing regions were analyzed to determine their sensitivity against botryticides. Fun...

journal_title：Applied and environmental microbiology

authors： Leroch M,Plesken C,Weber RW,Kauff F,Scalliet G,Hahn M

更新日期：2013-01-01 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:Hepatitis A virus disinfection was assessed on contaminated stainless-steel disks. Ten microliters of fecally suspended hepatitis A virus was deposited on the center of each disk, dried for 20 min, and then covered with 20 microliters of the test product for 1 min. Of the 20 formulations tested, only 2% glutaraldehyde...

journal_title：Applied and environmental microbiology

authors： Mbithi JN,Springthorpe VS,Sattar SA

更新日期：1990-11-01 00:00:00

abstract：:The killing of Listeria monocytogenes following exposure to low pH, organic acids, and osmotic stress was enhanced by the addition of 5% (vol/vol) ethanol. At pH 3, for example, the presence of this agent stimulated killing by more than 3 log units in 40 min of exposure. The rate of cell death at pH 3.0 was dependent ...

journal_title：Applied and environmental microbiology

authors： Barker C,Park SF

更新日期：2001-04-01 00:00:00

abstract：:The aerobic intestinal microflora of 2-week-old herring (Clupea harengus) larvae was characterized by using conventional microbiological methods and electron microscopy. Larvae were hatched and kept in filtered seawater or in seawater with penicillin and streptomycin. The gastrointestinal tract of herring larvae is es...

journal_title：Applied and environmental microbiology

authors： Hansen GH,Strøm E,Olafsen JA

更新日期：1992-02-01 00:00:00

abstract：:Because Bacteroides spp. are obligate anaerobes that dominate the human fecal flora, and because some species may live only in the human intestine, these bacteria might be useful to distinguish human from nonhuman sources of fecal pollution. To test this hypothesis, PCR primers specific for 16S rRNA gene sequences of ...

journal_title：Applied and environmental microbiology

authors： Kreader CA

更新日期：1995-04-01 00:00:00