Quantitative detection of the free-living amoeba Hartmannella vermiformis in surface water by using real-time PCR.

abstract：:In Europe, Borrelia burgdorferi genospecies causing Lyme borreliosis are mainly transmitted by the tick Ixodes ricinus. Since its discovery, B. burgdorferi has been the subject of many epidemiological studies to determine its prevalence and the distribution of the different genospecies in ticks. In the current study w...

journal_title：Applied and environmental microbiology

authors： Rauter C,Hartung T

更新日期：2005-11-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:Cholera is a severe diarrheal disease caused by specific serogroups of Vibrio cholerae that are pathogenic to humans. The disease does not persist in a chronic state in humans or animals. The pathogen is naturally present as a free-living organism in the environment. Recently, it was suggested that egg masses of the n...

journal_title：Applied and environmental microbiology

authors： Halpern M,Gancz H,Broza M,Kashi Y

更新日期：2003-07-01 00:00:00

abstract：:Extraintestinal growth of fecal bacteria can impair accurate assessment of watershed health. Anaerobic fecal bacteria belonging to the order Bacteroidales are attractive candidates for fecal source tracking because they have host-specific distributions and do not grow well in the presence of high oxygen concentrations...

journal_title：Applied and environmental microbiology

authors： Walters SP,Field KG

更新日期：2006-07-01 00:00:00

abstract：:Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

journal_title：Applied and environmental microbiology

authors： Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

更新日期：2012-02-01 00:00:00

abstract：:PCR-based methods have been developed to rapidly screen for Legionella pneumophila in water as an alternative to time-consuming culture techniques. However, these methods fail to discriminate between live and dead bacteria. Here, we report a viability assay (viability PCR [v-PCR]) for L. pneumophila that combines ethi...

journal_title：Applied and environmental microbiology

authors： Delgado-Viscogliosi P,Solignac L,Delattre JM

更新日期：2009-06-01 00:00:00

abstract：:The purpose of this investigation was to examine the capacity of the biphenyl catabolic enzymes of Comamonas testosteroni B-356 to metabolize dihydroxybiphenyls symmetrically substituted on both rings. Data show that 3,3'-dihydroxybiphenyl is by far the preferred substrate for strain B-356. However, the dihydrodiol me...

journal_title：Applied and environmental microbiology

authors： Sondossi M,Barriault D,Sylvestre M

更新日期：2004-01-01 00:00:00

abstract：:Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

journal_title：Applied and environmental microbiology

authors： Presley GN,Panisko E,Purvine SO,Schilling JS

更新日期：2018-08-01 00:00:00

abstract：:Antigens from the methicillin-resistant Staphylococcus aureus (MRSA) cell wall have been shown to be immunogenic in vivo and upregulated during biofilm growth. In this study, we created purified, recombinant forms of selected antigens and biofilm-upregulated, cell wall-associated proteins. These proteins were shown to...

journal_title：Applied and environmental microbiology

authors： Brady RA,Leid JG,Kofonow J,Costerton JW,Shirtliff ME

更新日期：2007-10-01 00:00:00

abstract：:In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...

journal_title：Applied and environmental microbiology

authors： Pontes MH,Dale C

更新日期：2011-03-01 00:00:00

abstract：:Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...

journal_title：Applied and environmental microbiology

authors： Voordouw G,Armstrong SM,Reimer MF,Fouts B,Telang AJ,Shen Y,Gevertz D

更新日期：1996-05-01 00:00:00

abstract：:Human noroviruses (HuNoVs) are the leading cause of food-borne illness, accounting for 58% of U.S. cases. Because HuNoVs are unculturable, surrogates are needed to investigate transmission routes and evaluate disinfection methods. However, the current surrogates, feline calicivirus (FCV) and murine NoV (MNV), are less...

journal_title：Applied and environmental microbiology

authors： Wang Q,Zhang Z,Saif LJ

更新日期：2012-06-01 00:00:00

abstract：:To ascertain its physiological similarity to other methanogenic bacteria, Methanospirillum hungatii, the type species of the genus, was characterized nutritionally and biochemically. Good growth occurred in a medium consisting of mineral salts, cysteine sulfide reducing buffer, and an H2-CO2 (80:20) atmosphere. Additi...

journal_title：Applied and environmental microbiology

authors： Ferry JG,Wolfe RS

更新日期：1977-10-01 00:00:00

abstract：:The MICRO-ID LISTERIA system, designed to identify Listeria isolates to species level within 24 h, was compared with conventional biochemical identification. MICRO-ID LISTERIA used in combination with the CAMP test correctly identified 409 (98.8%) of 414 strains isolated from human, animal, food, and environmental sou...

journal_title：Applied and environmental microbiology

authors： Bannerman E,Yersin MN,Bille J

更新日期：1992-06-01 00:00:00

abstract：:Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

journal_title：Applied and environmental microbiology

authors： Wyman M,Davies JT,Crawford DW,Purdie DA

更新日期：2000-06-01 00:00:00

abstract：:Thiolase (acetyl coenzyme A acetyltransferase; EC 2.3.1.9) from Clostridium acetobutylicum is a key enzyme in the production of acids and solvents in this organism. The purification and properties of the enzyme have already been described (D. P. Wiesenborn, F. B. Rudolph, and E.T. Papoutsakis, Appl. Environ. Microbiol...

journal_title：Applied and environmental microbiology

authors： Petersen DJ,Bennett GN

更新日期：1991-09-01 00:00:00

abstract：:With the submersible JAGO and by scuba diving we discovered three remarkable geothermal cones, rising 33, 25, and 45 m from the seafloor at a depth of 65 m in Eyjafjordur, northern Iceland. The greatest geothermal activity was on the highest cone, which discharged up to 50 liters of freshwater per s at 72 degrees C an...

journal_title：Applied and environmental microbiology

authors： Marteinsson VT,Kristjánsson JK,Kristmannsdóttir H,Dahlkvist M,Saemundsson K,Hannington M,Pétursdóttir SK,Geptner A,Stoffers P

更新日期：2001-02-01 00:00:00

abstract：:NAD-linked alcohol dehydrogenase activity was detected in cell-free crude extracts from various propane-grown bacteria. Two NAD-linked alcohol dehydrogenases, one which preferred primary alcohols (alcohol dehydrogenase I) and another which preferred secondary alcohols (alcohol dehydrogenase II), were found in propane-...

journal_title：Applied and environmental microbiology

authors： Hou CT,Patel RN,Laskin AI,Barist I,Barnabe N

更新日期：1983-07-01 00:00:00

abstract：:Water delivered by dental unit water systems (DUWS) in general dental practices can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWS provide a reservoir for microorganisms and should be controlled. This study compared disinfection products for their ability to meet the...

journal_title：Applied and environmental microbiology

authors： Schel AJ,Marsh PD,Bradshaw DJ,Finney M,Fulford MR,Frandsen E,Østergaard E,ten Cate JM,Moorer WR,Mavridou A,Kamma JJ,Mandilara G,Stösser L,Kneist S,Araujo R,Contreras N,Goroncy-Bermes P,O'Mullane D,Burke F,O'Reilly P

更新日期：2006-02-01 00:00:00

abstract：:The occurrence and distribution of magnetotactic bacteria (MB) were studied as a function of the physical and chemical conditions in meromictic Salt Pond, Falmouth, Mass., throughout summer 2002. Three dominant MB morphotypes were observed to occur within the chemocline. Small microaerophilic magnetite-producing cocci...

journal_title：Applied and environmental microbiology

authors： Simmons SL,Sievert SM,Frankel RB,Bazylinski DA,Edwards KJ

更新日期：2004-10-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:Kraft pulps, prepared from softwoods, and small chips of birch wood were treated with heme and tert-butyl hydroperoxide in aqueous solutions at reflux temperature. Analyses of treated pulps showed decreases in kappa number (a measure of lignin content) from about 36 to less than 2, with concomitant increases in bright...

journal_title：Applied and environmental microbiology

authors： Paszczyński A,Crawford RL,Blanchette RA

更新日期：1988-01-01 00:00:00

abstract：:Rhodococcus erythropolis HL 24-1 isolated as a 2,4-dinitrophenol-degrading organism can utilize 2-chloro-4,6-dinitrophenol as the sole nitrogen, carbon, and energy source under aerobic conditions. This compound is metabolized with liberation of stoichiometric amounts of chloride and nitrite. Under anaerobic conditions...

journal_title：Applied and environmental microbiology

authors： Lenke H,Knackmuss H

更新日期：1996-03-01 00:00:00

abstract：:Giardia muris cysts were separated from fecal contaminants in primary isolates by unit gravity velocity sedimentation. Crude isolates obtained by centrifugation over 1.0 M sucrose were overlaid onto a Percoll density gradient, 1.01 to 1.03 g/ml. G. muris cysts were well separated from faster-sedimenting fecal debris a...

journal_title：Applied and environmental microbiology

authors： Sauch JF

更新日期：1984-08-01 00:00:00

abstract：:The fate and impact of Pseudomonas aureofaciens TX-1 following application as a biocontrol agent for fungi in turfgrass were studied. The organism was applied with a modified irrigation system by using a preparation containing 1 x 10(6) P. aureofaciens TX-1 CFU ml(-1) about 100 times between May and August. We examine...

journal_title：Applied and environmental microbiology

authors： Sigler WV,Nakatsu CH,Reicher ZJ,Turco RF

更新日期：2001-08-01 00:00:00

abstract：:Cytochrome P450 BM-3, a self-sufficient P450 enzyme from Bacillus megaterium that catalyzes the subterminal hydroxylation of long-chain fatty acids, has been engineered into a catalyst for the oxidation of polycyclic aromatic hydrocarbons. The activities of a triplet mutant (A74G/F87V/L188Q) towards naphthalene, fluor...

journal_title：Applied and environmental microbiology

authors： Li QS,Ogawa J,Schmid RD,Shimizu S

更新日期：2001-12-01 00:00:00

abstract：:A light cycler-based real-time PCR (LC-PCR) assay that amplifies the F57 sequence of Mycobacterium avium subsp. paratuberculosis was developed. This assay also includes an internal amplification control template to monitor the amplification conditions in each reaction. The targeted F57 sequence element is unique for M...

journal_title：Applied and environmental microbiology

authors： Tasara T,Stephan R

更新日期：2005-10-01 00:00:00

abstract：:Of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. The isolates were characterized as Aeromonas sp., Chromobacterium cf. lividum, Vibrio sp., and two Pseudomonas spp. Specific antibodies were produced by immunizatio...

journal_title：Applied and environmental microbiology

authors： Dahle AB,Laake M

更新日期：1982-01-01 00:00:00

abstract：:Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substr...

journal_title：Applied and environmental microbiology

authors： Tsuchiya K,Kimura T

更新日期：1978-04-01 00:00:00

abstract：:Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a repo...

journal_title：Applied and environmental microbiology

authors： Mach RL,Peterbauer CK,Payer K,Jaksits S,Woo SL,Zeilinger S,Kullnig CM,Lorito M,Kubicek CP

更新日期：1999-05-01 00:00:00