A novel real-time PCR for Listeria monocytogenes that monitors analytical performance via an internal amplification control.

abstract：:Trichoderma species isolated from water-damaged buildings were screened for toxicity by using boar sperm cells as indicator cells. The crude methanolic cell extract from Trichoderma harzianum strain ES39 inhibited the boar sperm cell motility at a low exposure concentration (50% effective concentration, 1 to 5 microg ...

journal_title：Applied and environmental microbiology

authors： Peltola J,Ritieni A,Mikkola R,Grigoriev PA,Pócsfalvi G,Andersson MA,Salkinoja-Salonen MS

更新日期：2004-08-01 00:00:00

abstract：:To overcome the limited availability of antibiotic resistance markers in filamentous fungi, we adapted the FLP/FRT recombination system from the yeast Saccharomyces cerevisiae for marker recycling. We tested this system in the penicillin producer Penicillium chrysogenum using different experimental approaches. In a tw...

journal_title：Applied and environmental microbiology

authors： Kopke K,Hoff B,Kück U

更新日期：2010-07-01 00:00:00

abstract：:Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

journal_title：Applied and environmental microbiology

authors： Gandhi KM,Mandrell RE,Tian P

更新日期：2010-12-01 00:00:00

abstract：:A combination of inhibitors and carbon substrates was used to determine the relative contribution of sulfate-reducing bacteria (SRB) and fermenting bacteria to nitrogen fixation in a salt marsh sediment and on the roots of Spartina alterniflora. Because a lag period precedes acetylene-reducing activity (ARA) in amende...

journal_title：Applied and environmental microbiology

authors： Gandy EL,Yoch DC

更新日期：1988-08-01 00:00:00

abstract：:A total of 85 isolates of mesophilic, aerobic, heterotrophic bacteria were isolated from the gut, peristomial membrane, and coelomic fluid from specimens of the sea urchin Echinus esculentus from the Clyde Sea area of Scotland. These isolates were compared with 26 isolates from sand and seawater in the same locality. ...

journal_title：Applied and environmental microbiology

authors： Unkles SE

更新日期：1977-10-01 00:00:00

abstract：:Soil samples were screened to select microorganisms with the capability to oxidize organic sulfides into the corresponding sulfoxides with differential enantioselectivities. Several bacterial strains that preferentially produced the S-configured sulfoxide enantiomer were isolated. Surprisingly, one bacterial strain, g...

journal_title：Applied and environmental microbiology

authors： Adam W,Heckel F,Saha-Möller CR,Taupp M,Meyer JM,Schreier P

更新日期：2005-04-01 00:00:00

abstract：:Seventeen bacteriophages, active against 19 Arthrobacter soil isolates, were isolated from concentrated samples of river water and sewage. Attempts to isolate Arthrobacter bacteriophages from filtrates of broth cultures of the soil isolates or from ultraviolet light-irradiated cultures were unsuccessful. Bacteriophage...

journal_title：Applied and environmental microbiology

authors： Brown DR,Holt JG,Pattee PA

更新日期：1978-01-01 00:00:00

abstract：:The ability of certain oral biofilm bacteria to moderate pH through arginine metabolism by the arginine deiminase system (ADS) is a deterrent to the development of dental caries. Here, we characterize a novel Streptococcus strain, designated strain A12, isolated from supragingival dental plaque of a caries-free indivi...

journal_title：Applied and environmental microbiology

authors： Huang X,Palmer SR,Ahn SJ,Richards VP,Williams ML,Nascimento MM,Burne RA

更新日期：2016-01-29 00:00:00

abstract：:The spore-forming bacterium Paenibacillus larvae causes a severe and highly infective bee disease, American foulbrood (AFB). Despite the large economic losses induced by AFB, the virulence factors produced by P. larvae are as yet unknown. To identify such virulence factors, we experimentally infected young, susceptibl...

journal_title：Applied and environmental microbiology

authors： Schild HA,Fuchs SW,Bode HB,Grünewald B

更新日期：2014-04-01 00:00:00

abstract：:Thirty-eight cultures of Legionella pneumophila isolated from surface waters were characterized by their morphological, tinctorial, biochemical, and serological properties and by their ability to produce disease in guinea pigs. Their susceptibility to antimicrobial agents also was tested. When they were compared with...

journal_title：Applied and environmental microbiology

authors： Orrison LH,Cherry WB,Fliermans CB,Dees SB,McDougal LK,Dodd DJ

更新日期：1981-07-01 00:00:00

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00

abstract：:Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reducti...

journal_title：Applied and environmental microbiology

authors： Woo IS,Rhee IK,Park HD

更新日期：2000-05-01 00:00:00

abstract：:Biotransformation of 2-chlorophenol by a methanogenic sediment community resulted in the transient accumulation of phenol and benzoate. 3-Chlorobenzoate was a more persistent product of 2-chlorophenol metabolism. The anaerobic biotransformation of phenol to benzoate presumably occurred via para-carboxylation and dehyd...

journal_title：Applied and environmental microbiology

authors： Becker JG,Stahl DA,Rittmann BE

更新日期：1999-11-01 00:00:00

abstract：:Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, tran...

journal_title：Applied and environmental microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-07-01 00:00:00

abstract：:Large-scale composting facilities are known to cause environmental problems, mainly through pungent air emitted by composting material. In air samples taken above stacks set up to prepare compost used as a substrate in mushroom cultivation, several volatile compounds were identified by means of the coupled techniques ...

journal_title：Applied and environmental microbiology

authors： Derikx PJ,Op Den Camp HJ,van der Drift C,van Griensven LJ,Vogels GD

更新日期：1990-01-01 00:00:00

abstract：:Decomposition of natural populations of Lake Mendota phytoplankton dominated by blue-green algae (cyanobacteria) was monitored by using oxygen uptake and disappearance of chlorophyll, algal volume (fluorescence microscopy), particulate protein, particulate organic carbon, and photosynthetic ability (CO(2) up-take). In...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Brock TD

更新日期：1979-05-01 00:00:00

abstract：:Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Sa...

journal_title：Applied and environmental microbiology

authors： Kim HJ,Park SH,Kim HY

更新日期：2006-09-01 00:00:00

abstract：:The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...

journal_title：Applied and environmental microbiology

authors： Halos L,Jamal T,Maillard R,Girard B,Guillot J,Chomel B,Vayssier-Taussat M,Boulouis HJ

更新日期：2004-10-01 00:00:00

abstract：:Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PC...

journal_title：Applied and environmental microbiology

authors： Weyman PD,Pratte B,Thiel T

更新日期：2008-04-01 00:00:00

abstract：:A method was developed to deposit Bacillus subtilis spores via aerosolization onto various surface materials for biological agent decontamination and detection studies. This new method uses an apparatus coupled with a metered dose inhaler to reproducibly deposit spores onto various surfaces. A metered dose inhaler was...

journal_title：Applied and environmental microbiology

authors： Lee SD,Ryan SP,Snyder EG

更新日期：2011-03-01 00:00:00

abstract：:Endotoxin exposure occurs in homes and occupational environments and is known to cause adverse health effects. In order to compare results from different studies and establish standards, airborne endotoxin exposures should be assessed using standardized methods. Although the European Committee for Standardization (CEN...

journal_title：Applied and environmental microbiology

authors： Spaan S,Heederik DJ,Thorne PS,Wouters IM

更新日期：2007-10-01 00:00:00

abstract：:Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...

journal_title：Applied and environmental microbiology

authors： Ganeshnarayan K,Shah SM,Libera MR,Santostefano A,Kaplan JB

更新日期：2009-03-01 00:00:00

abstract：:Samples of a sandy loam soil taken from a long-term liming experiment in southeast England were amended with solutions of metal sulfate salts. Soils with a range of pHs were amended to contain Cu, Cd, or Zn at concentrations around the maximum permissible values for these metals in agricultural land receiving sewage s...

journal_title：Applied and environmental microbiology

authors： Knight BP,McGrath SP,Chaudri AM

更新日期：1997-01-01 00:00:00

abstract：:Alfalfa sprouts and other seed sprouts have been implicated in numerous outbreaks of salmonellosis. The source of these epidemics appears to have been low-level contamination of seeds by Salmonella bacteria that developed into clinically significant populations during the seed germination process. To test the possibil...

journal_title：Applied and environmental microbiology

authors： Howard MB,Hutcheson SW

更新日期：2003-01-01 00:00:00

abstract：:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) ...

journal_title：Applied and environmental microbiology

authors： Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

更新日期：2008-09-01 00:00:00

abstract：:Water samples from rivers, streams, ponds, and activated sewage were tested for the presence of bacteria which utilize 2,4-dichlorophenoxyacetic acid (2,4-D) as a sole source of carbon. Seventy percent of the attempted enrichments yielded pure cultures of 2,4-D-metabolizing bacteria. All but 1 of the 30 isolates were ...

journal_title：Applied and environmental microbiology

authors： Amy PS,Schulke JW,Frazier LM,Seidler RJ

更新日期：1985-05-01 00:00:00

abstract：:The dairy starter bacterium Lactococcus lactis is able to synthesize folate and accumulates large amounts of folate, predominantly in the polyglutamyl form. Only small amounts of the produced folate are released in the extracellular medium. Five genes involved in folate biosynthesis were identified in a folate gene cl...

journal_title：Applied and environmental microbiology

authors： Sybesma W,Starrenburg M,Kleerebezem M,Mierau I,de Vos WM,Hugenholtz J

更新日期：2003-06-01 00:00:00

abstract：:Divercin V41 (DV41) is a class IIa bacteriocin produced by Carnobacterium divergens V41. This antilisterial peptide is homologous to pediocin PA-1 and contains two disulfide bonds. To establish the structure-activity relationships of this specific family of bacteriocin, chemical modifications and enzymatic hydrolysis ...

journal_title：Applied and environmental microbiology

authors： Bhugaloo-Vial P,Douliez JP,Moll D,Dousset X,Boyaval P,Marion D

更新日期：1999-07-01 00:00:00

abstract：:We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of ap...

journal_title：Applied and environmental microbiology

authors： Stepkowski T,Moulin L,Krzyzańska A,McInnes A,Law IJ,Howieson J

更新日期：2005-11-01 00:00:00

abstract：:Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. Newly isolated Enterobacter aerogenes strain AJ110637, a rapid carbon source assimilator under weakly acidic (pH 5.0) conditions, was selected as a platform for succinate production. Our previo...

journal_title：Applied and environmental microbiology

authors： Tajima Y,Yamamoto Y,Fukui K,Nishio Y,Hashiguchi K,Usuda Y,Sode K

更新日期：2015-02-01 00:00:00