Production and properties of cyanobacterial endotoxins.

abstract：:Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

journal_title：Applied and environmental microbiology

authors： Wyman M,Davies JT,Crawford DW,Purdie DA

更新日期：2000-06-01 00:00:00

abstract：:Citrate and pyruvate metabolism by nongrowing cells of Leuconostoc oenos was investigated. (sup13)C nuclear magnetic resonance (NMR) spectroscopy was used to elucidate the pathway of citrate breakdown and to probe citrate or pyruvate utilization, noninvasively, in living cell suspensions. The utilization of isotopical...

journal_title：Applied and environmental microbiology

authors： Ramos A,Lolkema JS,Konings WN,Santos H

更新日期：1995-04-01 00:00:00

abstract：:Aminopolycarboxylic acids are ubiquitous in natural waters and wastewaters. They have the ability to form very stable water-soluble complexes with many metallic di- or trivalent ions. The iron complex nitrilotriacetic acid-Fe(III) (FeNTA) has been previously shown to increase drastically the rate of photo- and biodegr...

journal_title：Applied and environmental microbiology

authors： Bunescu A,Besse-Hoggan P,Sancelme M,Mailhot G,Delort AM

更新日期：2008-10-01 00:00:00

abstract：:The spirochete Borrelia burgdorferi is the causative agent of Lyme disease, the leading vector-borne illness in the United States. Many of the genetic factors affecting spirochete morphology and physiology are unknown due to the limited genetic tools available and the large number of open reading frames with unknown f...

journal_title：Applied and environmental microbiology

authors： Stewart PE,Hoff J,Fischer E,Krum JG,Rosa PA

更新日期：2004-10-01 00:00:00

abstract：:Four soil temperature and moisture treatment regimens were imposed on Florunner peanuts 94 days after planting in experimental plots in 1980. At harvest (145 days after planting), the incidence of the Aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. Extensive colonization of s...

journal_title：Applied and environmental microbiology

authors： Hill RA,Blankenship PD,Cole RJ,Sanders TH

更新日期：1983-02-01 00:00:00

abstract：:The neutral red (NR) cell viability assay was used with various cell types of human origin to quantitate the potency of T-2 mycotoxin and its metabolites. The human melanoma SK-Mel/27 cell line was the most sensitive, with a midpoint cytotoxicity value of 2.8 ng of T-2 per ml. With the human hepatoma cell line, HepG2,...

journal_title：Applied and environmental microbiology

authors： Babich H,Borenfreund E

更新日期：1991-07-01 00:00:00

abstract：:Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. Conventional farm sa...

journal_title：Applied and environmental microbiology

authors： Jindal A,Kocherginskaya S,Mehboob A,Robert M,Mackie RI,Raskin L,Zilles JL

更新日期：2006-12-01 00:00:00

abstract：:A procedure was developed to isolate and determine ergot alkaloid production by Acremonium coenophialum, the endophytic fungus of tall fescue. The procedure established that macerated leaf sheath or pith from inflorescence stem placed either in a liquid medium or on a corn meal-malt extract agar medium produced isolat...

journal_title：Applied and environmental microbiology

authors： Bacon CW

更新日期：1988-11-01 00:00:00

abstract：:Through genome-wide transcript analysis of a reference strain and two recombinant Saccharomyces cerevisiae strains with different rates of galactose uptake, we obtained information about the global transcriptional response to metabolic engineering of the GAL gene regulatory network. One of the recombinant strains over...

journal_title：Applied and environmental microbiology

authors： Bro C,Knudsen S,Regenberg B,Olsson L,Nielsen J

更新日期：2005-11-01 00:00:00

abstract：:A survey was made to detect the sensitivity of 12 yeast genera to T-2 toxin. Seventy-five yeasts isolated from various sources were tested for their susceptibility to T-2 toxin. The MIC of T-2 for these yeasts varied from 1.0 to greater than 8.0 micrograms/ml. Of the yeasts studied, Kluyveromyces fragilis showed the g...

journal_title：Applied and environmental microbiology

authors： Sukroongreung S,Schappert KT,Khachatourians GG

更新日期：1984-08-01 00:00:00

abstract：:Isolates of Gaeumannomyces graminis var. tritici, the causal agent of take-all of wheat, varied in sensitivity in vitro to the antibiotics phenazine-1-carboxylic acid (PCA) and 2,4-diacetylphloroglucinol (Phl) produced by fluorescent Pseudomonas spp. shown previously to have potential for biological control of this pa...

journal_title：Applied and environmental microbiology

authors： Mazzola M,Fujimoto DK,Thomashow LS,Cook RJ

更新日期：1995-07-01 00:00:00

abstract：:Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pse...

journal_title：Applied and environmental microbiology

authors： Wutipraditkul N,Waditee R,Incharoensakdi A,Hibino T,Tanaka Y,Nakamura T,Shikata M,Takabe T,Takabe T

更新日期：2005-08-01 00:00:00

abstract：:Under anoxic conditions, most methoxylated mononuclear aromatic compounds are degraded by bacteria, with catechol being formed as an important intermediate. On the basis of our experiments with the sulfate-reducing bacterium Desulfobacterium sp. strain Cat2, we describe for the first time the enzymatic activities invo...

journal_title：Applied and environmental microbiology

authors： Gorny N,Schink B

更新日期：1994-09-01 00:00:00

abstract：:Sulfidogenic consortia enriched from an estuarine sediment were maintained on either 2-, 3-, or 4-chlorophenol as the only source of carbon and energy for over 5 years. The enrichment culture on 4-chlorophenol was the most active and this consortium was selected for further characterization. Utilization of chloropheno...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Young LY

更新日期：1995-04-01 00:00:00

abstract：:During the fermentation of sake, cells of Saccharomyces cerevisiae are exposed to high concentrations of ethanol, thereby damaging the cell membrane and functional proteins. L-proline protects yeast cells from damage caused by freezing or oxidative stress. In this study, we evaluated the role of intracellular L-prolin...

journal_title：Applied and environmental microbiology

authors： Takagi H,Takaoka M,Kawaguchi A,Kubo Y

更新日期：2005-12-01 00:00:00

abstract：:Environmental contamination with compounds containing oxyanions of chlorine, such as perchlorate or chlorate [(per)chlorate] or chlorine dioxide, has been a constantly growing problem over the last 100 years. Although the fact that microbes reduce these compounds has been recognized for more than 50 years, only six or...

journal_title：Applied and environmental microbiology

authors： Coates JD,Michaelidou U,Bruce RA,O'Connor SM,Crespi JN,Achenbach LA

更新日期：1999-12-01 00:00:00

abstract：:Fluorescent antibodies (FAs) prepared against 16 ammonia-oxidizing nitrifying bacteria were examined as to cross-reactivity in heterologous FA staining tests. Virtually all cross-reactions to the seven Nitrosomonas FAs were confined to the Nitrosomonas isolates. The five Nitrosospira, three Nitrosolobus, and one Nitro...

journal_title：Applied and environmental microbiology

authors： Belser LW,Schmidt EL

更新日期：1978-10-01 00:00:00

abstract：:The subunit composition of the extracellular complex from Clostridium thermocellum was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Twenty-six bands, representing proteins with apparent molecular sizes ranging from 37,500 to 185,000 Da, could be detected by silver staining. Cultiva...

journal_title：Applied and environmental microbiology

authors： Kohring S,Wiegel J,Mayer F

更新日期：1990-12-01 00:00:00

abstract：:Bacteriovorous protozoa harboring symbiotic algae are abundant in aquatic ecosystems, yet despite a recent interest in protozoan bacterivory, the influence of light on their ingestion rates has not been investigated. In this study, Paramecium bursaria containing endosymbiotic Chlorella was tested for the effect of lig...

journal_title：Applied and environmental microbiology

authors： Berk SG,Parks LH,Ting RS

更新日期：1991-08-01 00:00:00

abstract：:Mass spectrometric measurement of carbon dioxide production was used to study malolactic fermentation (MLF) in Lactobacillus collinoides isolated from cider. The kinetics and stereospecificity of the malolactic enzyme (MLE) were studied, and the stoichiometry of the reaction sequence was investigated. The optimum pH f...

journal_title：Applied and environmental microbiology

authors： Arthurs CE,Lloyd D

更新日期：1999-08-01 00:00:00

abstract：:A gene, pfa1, encoding an autotransporter was cloned from a pathogenic Pseudomonas fluorescens strain, TSS, isolated from diseased fish. The expression of pfa1 is enhanced during infection and is regulated by growth phase and growth conditions. Mutation of pfa1 significantly attenuates the overall bacterial virulence ...

journal_title：Applied and environmental microbiology

authors： Hu YH,Liu CS,Hou JH,Sun L

更新日期：2009-07-01 00:00:00

abstract：:The growth of marine bacteria under iron-limited conditions was investigated. Neither siderophore production nor bacterial growth was detected for Pelagiobacter sp. strain V0110 when Fe(III) was present in the culture medium at a concentration of <1.0 microM. However, the growth of V0110 was strongly stimulated by the...

journal_title：Applied and environmental microbiology

authors： Guan LL,Onuki H,Kamino K

更新日期：2000-07-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00

abstract：:Enterocytozoon bieneusi is an emerging and clinically significant enteric parasite infecting humans and animals and can cause life-threatening diarrhea in immunocompromised people. Pigs are considered to be one of the main reservoir hosts of E. bieneusi based on their high prevalence rates and zoonotic genotypes in pi...

journal_title：Applied and environmental microbiology

authors： Zhao W,Zhang W,Yang F,Cao J,Liu H,Yang D,Shen Y,Liu A

更新日期：2014-06-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:Nitrogen-fixing cyanobacteria such as Anabaena variabilis ATCC 29413 use an uptake hydrogenase, encoded by hupSL, to recycle hydrogen gas that is produced as an obligate by-product of nitrogen fixation. The regulation of hupSL in A. variabilis is likely to differ from that of the closely related Anabaena sp. strain PC...

journal_title：Applied and environmental microbiology

authors： Weyman PD,Pratte B,Thiel T

更新日期：2008-04-01 00:00:00

abstract：:l-Phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) from Rhodotorula rubra has been used in the commercial manufacture of l-phenylalanine from trans-cinnamic acid. In this study, R. graminis PAL was investigated. Mutant strain GX6000 was isolated after ethyl methanesulfonate mutagenesis of wild-type R. graminis GX5007 by ...

journal_title：Applied and environmental microbiology

authors： Orndorff SA,Costantino N,Stewart D,Durham DR

更新日期：1988-04-01 00:00:00

abstract：:Carnobacteriocin B2 is a well-characterized class II bacteriocin produced by a 61-kb plasmid from Carnobacterium piscicola LV17. Export of this bacteriocin is dependent on specific ABC (ATP-binding cassette) secretion proteins. Divergicin A is a strongly hydrophobic narrow-spectrum bacteriocin produced by a 3.4-kb pla...

journal_title：Applied and environmental microbiology

authors： McCormick JK,Worobo RW,Stiles ME

更新日期：1996-11-01 00:00:00

abstract：:Twenty-seven Shiga toxin-producing Escherichia coli (STEC) strains were isolated from 207 stx-positive French environmental samples. Ten of these strains were positive for stx(1), and 24 were positive for stx(2) (10 were positive for stx(2vh-a) or stx(2vh-b), 19 were positive for stx(2d), and 15 were positive for stx(...

journal_title：Applied and environmental microbiology

authors： Vernozy-Rozand C,Montet MP,Bertin Y,Trably F,Girardeau JP,Martin C,Livrelli V,Beutin L

更新日期：2004-04-01 00:00:00

abstract：:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

journal_title：Applied and environmental microbiology

authors： Jenneman GE,Montgomery AD,McInerney MJ

更新日期：1986-04-01 00:00:00