Abstract:
:The membrane proteins IIC and IID of the mannose phosphotransferase system (Man-PTS) together form a membrane-located complex that serves as a receptor for several different bacteriocins, including the pediocin-like class IIa bacteriocins and the class IIc bacteriocin lactococcin A. Bacterial strains sensitive to class IIa bacteriocins readily give rise to resistant mutants upon bacteriocin exposure. In the present study, we have therefore investigated lactococcin A-resistant mutants of Lactococcus lactis as well as natural food isolates of Listeria monocytogenes with different susceptibilities to class IIa bacteriocins. We found two major mechanisms of resistance. The first involves downregulation of Man-PTS gene expression, which takes place both in spontaneous resistant mutants and in natural resistant isolates. The second involves normal expression of the Man-PTS system, but the underlying mechanism of resistance for these cells is unknown. In some cases, the resistant phenotype was linked to a shift in the metabolism; i.e., reduced growth on glucose due to reduction in Man-PTS expression was accompanied by enhanced growth on another sugar, such as galactose. The implications of these findings in terms of metabolic heterogeneity are discussed.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kjos M,Nes IF,Diep DBdoi
10.1128/AEM.02602-10subject
Has Abstractpub_date
2011-05-01 00:00:00pages
3335-42issue
10eissn
0099-2240issn
1098-5336pii
AEM.02602-10journal_volume
77pub_type
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.64.8.2780-2787.1998
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.47.4.886-887.1984
更新日期:1984-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/AEM.31.1.11-20.1976
更新日期:1976-01-01 00:00:00
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更新日期:1993-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.6.1938-1942.1993
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.57.12.3558-3564.1991
更新日期:1991-12-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.35.1.185-191.1978
更新日期:1978-01-01 00:00:00
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更新日期:1999-03-01 00:00:00
abstract::Searles Lake occupies a closed basin harboring salt-saturated, alkaline brines that have exceptionally high concentrations of arsenic oxyanions. Strain SLAS-1(T) was previously isolated from Searles Lake (R. S. Oremland, T. R. Kulp, J. Switzer Blum, S. E. Hoeft, S. Baesman, L. G. Miller, and J. F. Stolz, Science 308:1...
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.2.297-305.1978
更新日期:1978-08-01 00:00:00