Abstract:
:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bulk spore production, several medium compositions, based on soy peptone, corn steep liquor, and meat peptone, were tested and yielded 10(sup7) spores per ml. A medium pH above 7.0, a low glucose concentration, and a sufficient concentration of protein favored the sporulation of C. bifermentans KMR-1.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Sembries S,Crawford RLdoi
10.1128/AEM.63.5.2100-2104.1997keywords:
subject
Has Abstractpub_date
1997-05-01 00:00:00pages
2100-4issue
5eissn
0099-2240issn
1098-5336journal_volume
63pub_type
杂志文章abstract::The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.10.5750-5755.2004
更新日期:2004-10-01 00:00:00
abstract::Ammonia oxidation is a major process in nitrogen cycling, and it plays a key role in nitrogen limited soil ecosystems such as those in the arctic. Although mm-scale spatial dependency of ammonia oxidizers has been investigated, little is known about the field-scale spatial dependency of aerobic ammonia oxidation proce...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06132-11
更新日期:2012-01-01 00:00:00
abstract::Gibberellins (GAs) are tetracyclic diterpenoid phytohormones that were first identified as secondary metabolites of the fungus Fusarium fujikuroi (teleomorph, Gibberella fujikuroi). GAs were also found in the cassava pathogen Sphaceloma manihoticola, but the spectrum of GAs differed from that in F. fujikuroi. In contr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00694-08
更新日期:2008-09-01 00:00:00
abstract::The human pathogen Vibrio vulnificus is the leading cause of seafood-related deaths in the United States. Strains are genotyped on the basis of alleles that correlate with isolation source, with clinical (C)-genotype strains being more often implicated in disease and environmental (E)-genotype strains being more frequ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00753-15
更新日期:2015-09-01 00:00:00
abstract::Actinomycete isolates from indoor air and dust in water-damaged schools and children's day care centers were tested for toxicity by using boar spermatozoa as an indicator. Toxicity was detected in extracts of four strains which caused a loss of sperm motility, and the 50% effective concentrations (EC50) were 10 to 63 ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.12.4767-4773.1998
更新日期:1998-12-01 00:00:00
abstract::Systematic environmental surveillance for poliovirus circulation has been conducted in Egypt since 2000. The surveillance has revealed three independent importations of wild-type poliovirus. In addition, several vaccine-derived polioviruses have been detected in various locations in Egypt. In addition to acute flaccid...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00491-12
更新日期:2012-08-01 00:00:00
abstract::Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of Na...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.3083-3087.2000
更新日期:2000-07-01 00:00:00
abstract::Listeria monocytogenes can change its cellular morphology from bacilli to cocci during the transition to the long-term-survival (LTS) phase. The LTS cells demonstrated increased baro- and thermotolerance compared to their vegetative counterparts. So far, the underlying mechanisms that trigger this morphological and ph...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00596-11
更新日期:2011-09-01 00:00:00
abstract::A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.5.3217-3227.2006
更新日期:2006-05-01 00:00:00
abstract::The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.10.6302-6305.2004
更新日期:2004-10-01 00:00:00
abstract::During a molecular phylogenetic survey of extremely acidic (pH < 1), metal-rich acid mine drainage habitats in the Richmond Mine at Iron Mountain, Calif., we detected 16S rRNA gene sequences of a novel bacterial group belonging to the order Rickettsiales in the Alphaproteobacteria. The closest known relatives of this ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.9.5512-5518.2003
更新日期:2003-09-01 00:00:00
abstract::Two methods were used to evaluate bacterial recovery from beef, pork, and lamb adipose tissue. Higher counts were obtained with a tissue removal and fluid agitation technique (shaking) than with surface swabbing, but only when bacterial levels were low. Bacterial recovery by both methods was unaffected by specie origi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.35.5.978-979.1978
更新日期:1978-05-01 00:00:00
abstract::Gordonia polyisoprenivorans strain VH2, a potent rubber-degrading actinomycete, harbors two latex clearing proteins (Lcps), which are known to be essential for the microbial degradation of rubber. However, biochemical information on the exact role of this protein in the degradation of polyisoprene was lacking. In this...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01502-14
更新日期:2014-09-01 00:00:00
abstract::Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combinati...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00824-18
更新日期:2018-08-17 00:00:00
abstract::Denitrification is an alternative type of anaerobic respiration in which nitrate is reduced to gaseous products via nitrite. The key step in this process is the reduction of nitrite to nitric oxide, which is catalyzed by two structurally different but functionally equivalent forms of nitrite reductase encoded by the n...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01992-08
更新日期:2009-02-01 00:00:00
abstract::SslI, a type II restriction endonuclease, was purified from Streptococcus salivarius subsp. thermophilus strain BSN 45. SslI is an isoschizomer of BstNI. SslI activity was maximum at pH 8.8, 0 to 50 mM NaCl, 2 to 8 mM Mg2+, and 42 degrees C. Activity against phage DNA in vitro was demonstrated. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.12.3677-3678.1991
更新日期:1991-12-01 00:00:00
abstract::Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infec...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01456-09
更新日期:2010-05-01 00:00:00
abstract::Microscopic techniques were used in conjunction with normal microbiological procedures to examine the development of the spoilage microflora on the skin of chicken carcasses held at 2 degrees C. Pigmented and nonpigmented psychrotrophic pseudomonads were the major spoilage bacteria isolated at all stages of storage ex...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.2.492-503.1981
更新日期:1981-02-01 00:00:00
abstract::Transfer of plasmid-borne antibiotic resistance genes in Escherichia coli wild-type strains is possible by transformation under naturally occurring conditions in oligotrophic, aquatic environments containing physiologic concentrations of calcium. In contrast, transformation is suppressed in nitrogen-rich body fluids l...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.1.440-443.2002
更新日期:2002-01-01 00:00:00
abstract::Glucose catabolite repression of tetralin catabolic genes in Rhodococcus sp. strain TFB was shown to be exerted by a protein homologous to transcriptional regulators of the cyclic AMP receptor (CRP)-FNR family. The protein was detected bound to putative CRP-like boxes localized at the promoters of the thnA1 and thnS g...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00700-12
更新日期:2012-08-01 00:00:00
abstract::Legionella pneumophila proliferates in freshwater environments at temperatures ranging from 25 to 45°C. To investigate the preference of different sequence types (ST) for a specific temperature range, growth of L. pneumophila serogroup 1 (SG1) ST1 (environmental strains), ST47, and ST62 (disease-associated strains) wa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01107-16
更新日期:2016-10-27 00:00:00
abstract::In the present work, we aimed at determining the relationship between the hepatitis A virus (HAV) numbers in imported frozen coquina clams involved in two hepatitis outbreaks, as well as the risk for human health. Due to HAV unculturability, a standardized TaqMan real-time reverse transcription-PCR controlling the vir...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01177-09
更新日期:2009-12-01 00:00:00
abstract::Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.3.597-603.1982
更新日期:1982-09-01 00:00:00
abstract::The effect of selenite on the growth rate and protein synthesis has been investigated in Rhodobacter sphaeroides. This photosynthetic bacterium efficiently reduces selenite with intracellular accumulation under both dark aerobic and anaerobic photosynthetic conditions. Addition of 1 mM selenite under these two growth ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.10.4440-4447.2001
更新日期:2001-10-01 00:00:00
abstract::Sakacin G is a 37-amino-acid-residue-long class IIa bacteriocin produced by Lactobacillus sake 2512, which is encoded by the duplicated structural genes skgA1 and skgA2. Sakacin G appears to be unique and seems to be an intermediate between pediocin-like bacteriocins, according to its double-disulfide bridges required...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.12.6416-6420.2002
更新日期:2002-12-01 00:00:00
abstract::[This corrects the article on p. 588 in vol. 60.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.6.2207-2207.1994
更新日期:1994-06-01 00:00:00
abstract::Both the wild type and an isogenic hydrogenase-negative mutant of Azorhizobium caulinodans growing ex planta on N(2) as the N source were studied in succinate-limited steady-state chemostat cultures under 0.2 to 3.0% dissolved O(2) tension. Production or consumption of O(2), H(2), and CO(2) was measured with an on-lin...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.6.1859-1866.1994
更新日期:1994-06-01 00:00:00
abstract::During microaerophilic growth of magnetic spirillum MS-1 on tartrate and nitrate, a maximal cell density was obtained at an initial oxygen partial pressure of 17 Pa. A transient accumulation of nitrous oxide and a 1:2 (mol/mol) stoichiometry between tartrate oxidation and nitrate reduction were observed, indicating th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.40.2.429-430.1980
更新日期:1980-08-01 00:00:00
abstract::The cry1-type genes of Bacillus thuringiensis represent the largest cry gene family, which contains 50 distinct holotypes. It is becoming more and more difficult to identify cry1-type genes using current methods because of the increasing number of cry1-type genes. In the present study, an improved PCR-restriction frag...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01983-13
更新日期:2013-11-01 00:00:00
abstract::Submicronic fungal fragments have been observed in in vitro aerosolization experiments. The occurrence of these particles has therefore been suggested to contribute to respiratory health problems observed in mold-contaminated indoor environments. However, the role of submicronic fragments in exacerbating adverse healt...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00929-15
更新日期:2015-09-01 00:00:00