Production of Clostridium bifermentans Spores as Inoculum for Bioremediation of Nitroaromatic Contaminants.

Abstract:

:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bulk spore production, several medium compositions, based on soy peptone, corn steep liquor, and meat peptone, were tested and yielded 10(sup7) spores per ml. A medium pH above 7.0, a low glucose concentration, and a sufficient concentration of protein favored the sporulation of C. bifermentans KMR-1.

journal_name

Appl Environ Microbiol

authors

Sembries S,Crawford RL

doi

10.1128/AEM.63.5.2100-2104.1997

keywords:

subject

Has Abstract

pub_date

1997-05-01 00:00:00

pages

2100-4

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

63

pub_type

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