Modeling the dynamic regulation of nitrogen fixation in the cyanobacterium Trichodesmium sp.

abstract：:The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the major ribotypes. DGGE profiles of the...

journal_title：Applied and environmental microbiology

authors： Favier CF,Vaughan EE,De Vos WM,Akkermans AD

更新日期：2002-01-01 00:00:00

abstract：:Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis strains were isolated from 503 soil samples collected from the five macroregions of the country. The characterization of the strain coll...

journal_title：Applied and environmental microbiology

authors： Bravo A,Sarabia S,Lopez L,Ontiveros H,Abarca C,Ortiz A,Ortiz M,Lina L,Villalobos FJ,Peña G,Nuñez-Valdez ME,Soberón M,Quintero R

更新日期：1998-12-01 00:00:00

abstract：:An amylolytic Lactobacillus plantarum silage strain with the starch-degrading ability displayed by Lactobacillus amylovorus was developed. An active fragment of the gene coding for alpha-amylase production in L. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain L. plantarum L...

journal_title：Applied and environmental microbiology

authors： Fitzsimons A,Hols P,Jore J,Leer RJ,O'Connell M,Delcour J

更新日期：1994-10-01 00:00:00

abstract：:The association of Vibrio cholerae with zooplankton has been suggested as an important factor in transmission of human epidemic cholera, and the ability to colonize zooplankton surfaces may play a role in the temporal variation and predominance of the two different serogroups (V. cholerae O1 El Tor and O139) in the aq...

journal_title：Applied and environmental microbiology

authors： Rawlings TK,Ruiz GM,Colwell RR

更新日期：2007-12-01 00:00:00

abstract：:We developed a method to purify Clostridium perfringens phospholipase C from a culture of recombinant Bacillus subtilis cells. This method consists of three purification steps, and it allowed us to obtain 6.2 mg of pure phospholipase C from 800 ml of culture. ...

journal_title：Applied and environmental microbiology

authors： Hirata Y,Minami J,Koyama M,Matsushita O,Katayama S,Jin F,Maeta H,Okabe A

更新日期：1995-11-01 00:00:00

abstract：:Twelve strains of different Bacteroides species were tested for their efficiency of detection of bacteriophages from sewage. The host range of several isolated phages was investigated. The results indicated that there was a high degree of strain specificity. Then, by using Bacteroides fragilis HSP 40 as the host, whic...

journal_title：Applied and environmental microbiology

authors： Tartera C,Jofre J

更新日期：1987-07-01 00:00:00

abstract：:Campylobacter infections are increasing and pose a serious public health problem in Denmark. Infections in humans and broiler flocks show similar seasonality, suggesting that climate may play a role in infection. We examined the effects of temperature, precipitation, relative humidity, and hours of sunlight on Campylo...

journal_title：Applied and environmental microbiology

authors： Patrick ME,Christiansen LE,Wainø M,Ethelberg S,Madsen H,Wegener HC

更新日期：2004-12-01 00:00:00

abstract：:Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of...

journal_title：Applied and environmental microbiology

authors： Klein PG,Juneja VK

更新日期：1997-11-01 00:00:00

abstract：:Toluene dioxygenase from Pseudomonas putida F1 has been implicated as an enzyme capable of degrading trichloroethylene. This has now been confirmed with Escherichia coli JM109(pDTG601) that contains the structural genes (todC1C2BA) of toluene dioxygenase under the control of the tac promoter. The extent of trichloroet...

journal_title：Applied and environmental microbiology

authors： Zylstra GJ,Wackett LP,Gibson DT

更新日期：1989-12-01 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00

abstract：:Photorhabdus luminescens is a bacterium which is mutualistic with entomophagous nematodes and which secretes high-molecular-weight toxin complexes following its release into the insect hemocoel upon nematode invasion. Thus, unlike other protein toxins from Bacillus thuringiensis (delta-endotoxins and Vip's), P. lumine...

journal_title：Applied and environmental microbiology

authors： Blackburn M,Golubeva E,Bowen D,Ffrench-Constant RH

更新日期：1998-08-01 00:00:00

abstract：:Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic microbes that are found in a broad range of aquatic environments. Although potentially significant to the microbial ecology and biogeochemistry of marine ecosystems, their abundance and genetic diversity and the environmental variables that regulate...

journal_title：Applied and environmental microbiology

authors： Ritchie AE,Johnson ZI

更新日期：2012-04-01 00:00:00

abstract：:We have shown that the plant pathogen Alternaria brassicicola exhibited very high susceptibility to ambruticin VS4 and to a lesser extent to the phenylpyrrole fungicide fludioxonil. These compounds are both derived from natural bacterial metabolites with antifungal properties and are thought to exert their toxicity by...

journal_title：Applied and environmental microbiology

authors： Dongo A,Bataillé-Simoneau N,Campion C,Guillemette T,Hamon B,Iacomi-Vasilescu B,Katz L,Simoneau P

更新日期：2009-01-01 00:00:00

abstract：:The blacklegged tick, Ixodes scapularis, is of significant public health importance as a vector of Borrelia burgdorferi, the agent of Lyme borreliosis. The timing of seasonal activity of each immature I. scapularis life stage relative to the next is critical for the maintenance of B. burgdorferi because larvae must fe...

journal_title：Applied and environmental microbiology

authors： Gatewood AG,Liebman KA,Vourc'h G,Bunikis J,Hamer SA,Cortinas R,Melton F,Cislo P,Kitron U,Tsao J,Barbour AG,Fish D,Diuk-Wasser MA

更新日期：2009-04-01 00:00:00

abstract：:The Pseudomonas genus contains a metabolically versatile group of organisms that are known to occupy numerous ecological niches, including the rhizosphere and endosphere of many plants. Their diversity influences the phylogenetic diversity and heterogeneity of these communities. On the basis of average amino acid iden...

journal_title：Applied and environmental microbiology

authors： Jun SR,Wassenaar TM,Nookaew I,Hauser L,Wanchai V,Land M,Timm CM,Lu TY,Schadt CW,Doktycz MJ,Pelletier DA,Ussery DW

更新日期：2015-10-30 00:00:00

abstract：:Methane (CH(4)) flux to the atmosphere is mitigated via microbial CH(4) oxidation in sediments and water. As arctic temperatures increase, understanding the effects of temperature on the activity and identity of methanotrophs in arctic lake sediments is important to predicting future CH(4) emissions. We used DNA-based...

journal_title：Applied and environmental microbiology

authors： He R,Wooller MJ,Pohlman JW,Quensen J,Tiedje JM,Leigh MB

更新日期：2012-07-01 00:00:00

abstract：:Understanding how species interaction and assembly processes structure the abundant and rare bacterial biospheres in soils is crucial for predicting how biodiversity influences ecosystem functioning. Here, we profiled the bacterial communities across a salt marsh ecosystem gradient to investigate the co-occurrence pat...

journal_title：Applied and environmental microbiology

authors： Du S,Dini-Andreote F,Zhang N,Liang C,Yao Z,Zhang H,Zhang D

更新日期：2020-06-17 00:00:00

abstract：:Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in...

journal_title：Applied and environmental microbiology

authors： Wegkamp A,Starrenburg M,de Vos WM,Hugenholtz J,Sybesma W

更新日期：2004-05-01 00:00:00

abstract：:Protease-negative variants were shown to outcompete the wild-type strains of Streptococcus cremoris E(8), HP, and Wg(2) at pH values higher than 6.0 in milk. For S. cremoris E(8) this process was studied in more detail. At lower pH values the wild type had a selective advantage. This pH-dependent selection was not fou...

journal_title：Applied and environmental microbiology

authors： Hugenholtz J,Splint R,Konings WN,Veldkamp H

更新日期：1987-02-01 00:00:00

abstract：:Coumarin concentrations that inhibited growth of Pythium sp. rapidly decreased the rate of incorporation of [14C]glucose into the mycelium. Coumarin also reduced drastically the carbohydrate and protein content of the cytoplasm and, to a lesser extent, the amino acid content and cell wall fraction. Enzymes related to ...

journal_title：Applied and environmental microbiology

authors： Neto FC,Dietrich SM

更新日期：1977-09-01 00:00:00

abstract：:We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

更新日期：2005-04-01 00:00:00

abstract：:Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

journal_title：Applied and environmental microbiology

authors： Zhou Z,Raskin L,Zilles JL

更新日期：2010-04-01 00:00:00

abstract：:ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants w...

journal_title：Applied and environmental microbiology

authors： Suzuki E,Ohkawa H,Moriya K,Matsubara T,Nagaike Y,Iwasaki I,Fujiwara S,Tsuzuki M,Nakamura Y

更新日期：2010-05-01 00:00:00

abstract：:The deoxyribonucleic acid (DNA) yield and deoxyribonuclease (DNase) activity of several yeasts were correlated. Debaryomyces castellii and Debaryomyces franciscae were found to contain active DNases which carry out DNA hydrolysis, whereas the amounts of DNA as determined by extraction with Sarkosyl buffer (pH 7.8) wer...

journal_title：Applied and environmental microbiology

authors： El-Nakhal H,Phaff HJ

更新日期：1981-06-01 00:00:00

abstract：:In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

更新日期：2015-10-01 00:00:00

abstract：:The proteolytic activity of Butyrivibrio fibrisolvens, a ubiquitously distributed bacterial species in the gastrointestinal tracts of ruminants and other mammals, was characterized. The relative proteolytic activity (micrograms of azocasein degraded per hour per milligram of protein) varied greatly with the strain: 0 ...

journal_title：Applied and environmental microbiology

authors： Cotta MA,Hespell RB

更新日期：1986-07-01 00:00:00

abstract：:A study of six child care centers was conducted to assess the microbiological quality of three food contact surfaces (one food serving surface and two food preparation surfaces) and one non-food contact surface (diaper changing surface) to determine the effectiveness of cleaning and sanitization procedures within the ...

journal_title：Applied and environmental microbiology

authors： Cosby CM,Costello CA,Morris WC,Haughton B,Devereaux MJ,Harte F,Davidson PM

更新日期：2008-11-01 00:00:00

abstract：:Consumption of poultry contaminated with Campylobacter jejuni is a risk factor for human gastrointestinal disease. The rational development of control strategies for Campylobacter within chickens requires an understanding of the colonization process at the molecular and population levels, both within and between hosts...

journal_title：Applied and environmental microbiology

authors： Coward C,van Diemen PM,Conlan AJ,Gog JR,Stevens MP,Jones MA,Maskell DJ

更新日期：2008-06-01 00:00:00

abstract：:Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

更新日期：1999-09-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00