Bacterial sampling techniques on beef, pork, and lamb adipose tissue.

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage. Because of their efficiency and simplicity, serine integrases are also used for divers...

journal_title：Applied and environmental microbiology

authors： Fogg PCM,Haley JA,Stark WM,Smith MCM

更新日期：2017-02-15 00:00:00

abstract：:The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

journal_title：Applied and environmental microbiology

authors： Ma JF,Naranjo J,Gerba CP

更新日期：1994-06-01 00:00:00

abstract：:Phages play a key role in the marine environment by regulating the transfer of energy between trophic levels and influencing global carbon and nutrient cycles. The diversity of marine phage communities remains difficult to characterize because of the lack of a signature gene common to all phages. Recent studies have d...

journal_title：Applied and environmental microbiology

authors： Goldsmith DB,Crosti G,Dwivedi B,McDaniel LD,Varsani A,Suttle CA,Weinbauer MG,Sandaa RA,Breitbart M

更新日期：2011-11-01 00:00:00

abstract：:Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with dif...

journal_title：Applied and environmental microbiology

authors： Muniz Mde M,Morais E Silva Tavares P,Meyer W,Nosanchuk JD,Zancope-Oliveira RM

更新日期：2010-07-01 00:00:00

abstract：:Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...

journal_title：Applied and environmental microbiology

authors： Gingras SP,Howard LV

更新日期：1980-02-01 00:00:00

abstract：:The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus bre...

journal_title：Applied and environmental microbiology

authors： Macfarlane S,Woodmansey EJ,Macfarlane GT

更新日期：2005-11-01 00:00:00

abstract：:Many cyanobacteria are known to tolerate environmental extremes. Motivated by an interest in selecting cyanobacteria for applications in space, we launched rocks from a limestone cliff in Beer, Devon, United Kingdom, containing an epilithic and endolithic rock-dwelling community of cyanobacteria into low Earth orbit (...

journal_title：Applied and environmental microbiology

authors： Olsson-Francis K,de la Torre R,Cockell CS

更新日期：2010-04-01 00:00:00

abstract：:Laboratory-grown strains of chemoautotrophic Thiomicrospira sp. strain L-12 and Thiobacillus neapolitanus produced cell carbon that was 24.6 to 25.1 ppt (24.6 to 25.1 mg/g) lower in C isotope abundance than the ambient source of carbon dioxide and bicarbonate. This degree of C isotope depletion was comparable to that ...

journal_title：Applied and environmental microbiology

authors： Ruby EG,Jannasch HW,Deuser WG

更新日期：1987-08-01 00:00:00

abstract：:The presence of enteroviruses in oysters and oyster-harvesting waters of the Texas Gulf coast was monitored over a period of 10 months. Viruses were detected in water and oyster samples obtained from areas both open and closed to shellfish harvesting. Viruses were detected periodically in waters that met current bacte...

journal_title：Applied and environmental microbiology

authors： Goyal SM,Gerba CP,Melnick JL

更新日期：1979-03-01 00:00:00

abstract：:Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcrip...

journal_title：Applied and environmental microbiology

authors： Skraber S,Langlet J,Kremer JR,Mossong J,De Landtsheer S,Even J,Muller CP,Hoffmann L,Cauchie HM

更新日期：2011-08-01 00:00:00

abstract：:The effect of CO2 availability on cell size, shape, and aggregation in continuous cultures of Candida utilis was studied in minimal medium with glucose or ethanol as the sole carbon and energy source. Enrichment with CO2 was achieved (i) by using the substrate with more C atoms, (ii) by using pure oxygen and thus decr...

journal_title：Applied and environmental microbiology

authors： Straskrabová V,Páca J,Králícková E

更新日期：1980-11-01 00:00:00

abstract：:Due to the complex microecology and microenvironment of dental plaque, novel caries prevention strategies require modulating the microbial communities ecologically and reducing the cariogenic properties effectively. Antimicrobial peptide GH12 reduced the lactic acid production and exopolysaccharide (EPS) synthesis of ...

journal_title：Applied and environmental microbiology

authors： Jiang W,Wang Y,Luo J,Chen X,Zeng Y,Li X,Feng Z,Zhang L

更新日期：2020-07-02 00:00:00

abstract：:Sakacin K is an antilisterial bacteriocin produced by Lactobacillus sake CTC 494, a strain isolated from Spanish dry fermented sausages. The biokinetics of cell growth and bacteriocin production of L. sake CTC 494 in vitro during laboratory fermentations were investigated by making use of MRS broth. The data obtained ...

journal_title：Applied and environmental microbiology

authors： Leroy F,de Vuyst L

更新日期：1999-03-01 00:00:00

abstract：:Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the prese...

journal_title：Applied and environmental microbiology

authors： Brinig MM,Lepp PW,Ouverney CC,Armitage GC,Relman DA

更新日期：2003-03-01 00:00:00

abstract：:The arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. For example, as little as 2.9 mM arginine added to acidified suspensions of Streptococcus sanguis at a pH of 4.0 resulted in ammonia production and protection against killing. The argini...

journal_title：Applied and environmental microbiology

authors： Casiano-Colón A,Marquis RE

更新日期：1988-06-01 00:00:00

abstract：:We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...

journal_title：Applied and environmental microbiology

authors： Johnsen AR,Bendixen K,Karlson U

更新日期：2002-06-01 00:00:00

abstract：:In all purification stages of a biological sewage treatment plant, phototrophic bacteria were detected by the method of viable cell counts. The predominant species identified belonged to the genus Rhodopseudomonas of purple nonsulfur bacteria. The number of phototrophic bacteria was highest in wastewater containing sl...

journal_title：Applied and environmental microbiology

authors： Siefert E,Irgens RL,Pfennig N

更新日期：1978-01-01 00:00:00

abstract：:To get insight into the limiting factors existing for the efficient production of fungal peroxidase in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase H8 (lipA) and manganese peroxidase (MnP) H4 (mnp1) genes in Aspergillus niger has been studied. For this purpose, a protease-defi...

journal_title：Applied and environmental microbiology

authors： Conesa A,van den Hondel CA,Punt PJ

更新日期：2000-07-01 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

journal_title：Applied and environmental microbiology

authors： Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

更新日期：1998-04-01 00:00:00

abstract：:Tannins are a diverse group of plant-produced, polyphenolic compounds with metal-chelating and antimicrobial properties that are prevalent in many soils. Using transcriptomics, we determined that tannic acid, a form of hydrolysable tannin, broadly affects the expression of genes involved in iron and zinc homeostases, ...

journal_title：Applied and environmental microbiology

authors： Lim CK,Penesyan A,Hassan KA,Loper JE,Paulsen IT

更新日期：2013-05-01 00:00:00

abstract：:The antifungal activity of cecropin A(2-8)-melittin(6-9) hybrid undecapeptides, previously reported as active against plant pathogenic bacteria, was studied. A set of 15 sequences was screened in vitro against Fusarium oxysporum, Penicillium expansum, Aspergillus niger, and Rhizopus stolonifer. Most compounds were hig...

journal_title：Applied and environmental microbiology

authors： Badosa E,Ferré R,Francés J,Bardají E,Feliu L,Planas M,Montesinos E

更新日期：2009-09-01 00:00:00

abstract：:Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method...

journal_title：Applied and environmental microbiology

authors： Krzeslak J,Gerritse G,van Merkerk R,Cool RH,Quax WJ

更新日期：2008-03-01 00:00:00

abstract：:The susceptibility of the Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection, and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IK...

journal_title：Applied and environmental microbiology

authors： Fernández-Aguilar X,Cabezón O,Granados JE,Frey J,Serrano E,Velarde R,Cano-Manuel FJ,Mentaberre G,Ráez-Bravo A,Fandos P,López-Olvera JR

更新日期：2017-07-17 00:00:00

abstract：:The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the major ribotypes. DGGE profiles of the...

journal_title：Applied and environmental microbiology

authors： Favier CF,Vaughan EE,De Vos WM,Akkermans AD

更新日期：2002-01-01 00:00:00

abstract：:The insertion sequence IS629, which is highly prevalent in Escherichia coli O157:H7 genomes, was found to be absent in O157:H- strains, which are on a divergent pathway in the emergence of O157:H7. Although O157:H- is deficient in IS629, it permits IS629 transposition, with an excision frequency higher than that of an...

journal_title：Applied and environmental microbiology

authors： Rump LV,Fischer M,González-Escalona N

更新日期：2011-07-01 00:00:00

abstract：:Cellulose is an abundant and renewable biopolymer that can be used for biofuel generation; however, structural entrapment with other cell wall components hinders enzyme-substrate interactions, a key bottleneck for ethanol production. Biomass is routinely subjected to treatments that facilitate cellulase-cellulose cont...

journal_title：Applied and environmental microbiology

authors： Wang H,Squina F,Segato F,Mort A,Lee D,Pappan K,Prade R

更新日期：2011-08-01 00:00:00

abstract：:Candida parapsilosis, C. tropicalis, and Torulopsis glabrata were the human-associated yeasts most frequently isolated from quahogs, oysters, and mussels collected from four estuarine areas along the northern shore of Long Island Sound. Some inconsistency and seasonal variation in the occurrence of these and other yea...

journal_title：Applied and environmental microbiology

authors： Buck JD,Bubucis PM,Combs TJ

更新日期：1977-02-01 00:00:00

abstract：:Vector competence refers to the ability of an arthropod to acquire, maintain, and successfully transmit a microbial pathogen. Tick-borne relapsing fever (TBRF) spirochetes are globally distributed pathogens, and most species are transmitted by argasid ticks of the genus Ornithodoros. A defining characteristic in vecto...

journal_title：Applied and environmental microbiology

authors： Krishnavajhala A,Armstrong BA,Lopez JE

更新日期：2018-10-17 00:00:00

abstract：:Flavonoids are 15-carbon plant secondary metabolites exuded in the rhizosphere that hosts several flavonoid-degrading bacteria. We studied flavonoid catabolism in a plant growth-promoting rhizobacterial strain of Pseudomonas by using a combination of biochemical and genetic approaches. Transposants carrying mini-Tn5gf...

journal_title：Applied and environmental microbiology

authors： Pillai BV,Swarup S

更新日期：2002-01-01 00:00:00