Abstract:
:The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen. Little is known of the composition and metabolic activities of these biofilms, although they are likely to play an important role in mucus breakdown. The aims of this study were to determine how intestinal bacteria colonize mucus and to study physiologic and enzymatic factors involved in the destruction of this glycoprotein. Colonization of mucin gels by fecal bacteria was studied in vitro, using a two-stage continuous culture system, simulating conditions of nutrient availability and limitation characteristic of the proximal (vessel 1) and distal (vessel 2) colon. The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes. Gel samples were also taken for analysis of mucin-degrading enzymes and measurements of residual mucin sugars. Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia. Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Macfarlane S,Woodmansey EJ,Macfarlane GTdoi
10.1128/AEM.71.11.7483-7492.2005keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
7483-92issue
11eissn
0099-2240issn
1098-5336pii
71/11/7483journal_volume
71pub_type
杂志文章abstract::Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.41.2.559-560.1981
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doi:10.1128/AEM.37.2.187-193.1979
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doi:10.1128/AEM.59.8.2404-2410.1993
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.47.4.768-774.1984
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journal_title:Applied and environmental microbiology
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更新日期:1986-08-01 00:00:00
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doi:10.1128/AEM.35.4.631-635.1978
更新日期:1978-04-01 00:00:00
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更新日期:2009-12-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.53.2.416-421.1987
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pub_type: 杂志文章
doi:10.1128/AEM.58.2.461-470.1992
更新日期:1992-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2017-12-15 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.8.2095-2097.1989
更新日期:1989-08-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1997-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.7.2600-2606.1997
更新日期:1997-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
abstract::Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing env...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00